David I Friedman

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Prophage induction is enhanced and required for renal disease and lethality in an EHEC mouse model
    Jessica S Tyler
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Pathog 9:e1003236. 2013
  2. doi request reprint Activation of a prophage-encoded tyrosine kinase by a heterologous infecting phage results in a self-inflicted abortive infection
    David I Friedman
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Microbiol 82:567-77. 2011
  3. ncbi request reprint Transcription antitermination: the lambda paradigm updated
    D I Friedman
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 620, USA
    Mol Microbiol 18:191-200. 1995
  4. ncbi request reprint Bacteriophage lambda: alive and well and still doing its thing
    D I Friedman
    Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor Michigan, Ann Arbor, Michigan 48109 0620, USA
    Curr Opin Microbiol 4:201-7. 2001
  5. pmc Evidence that the promoter can influence assembly of antitermination complexes at downstream RNA sites
    Ying Zhou
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
    J Bacteriol 188:2222-32. 2006
  6. pmc The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression
    Jessica S Tyler
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48103, USA
    J Bacteriol 186:7670-9. 2004
  7. pmc Pathogenesis of renal disease due to enterohemorrhagic Escherichia coli in germ-free mice
    Kathryn A Eaton
    Unit for Laboratory Animal Medicine, 018 Animal Research Facility, University of Michigan, Ann Arbor, MI 48109 0614, USA
    Infect Immun 76:3054-63. 2008
  8. ncbi request reprint Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein
    Ying Zhou
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
    J Mol Biol 318:1175-88. 2002
  9. ncbi request reprint Characterizing spontaneous induction of Stx encoding phages using a selectable reporter system
    Jonathan Livny
    Program in Cell and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Microbiol 51:1691-704. 2004
  10. ncbi request reprint Analyzing transcription antitermination in lambdoid phages encoding toxin genes
    Melody N Neely
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Methods Enzymol 371:418-38. 2003

Research Grants

Collaborators

Detail Information

Publications16

  1. pmc Prophage induction is enhanced and required for renal disease and lethality in an EHEC mouse model
    Jessica S Tyler
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Pathog 9:e1003236. 2013
    ..Since the induced population produces and releases Stx2, this result indicates that an activity in the intestine increases Stx2 production...
  2. doi request reprint Activation of a prophage-encoded tyrosine kinase by a heterologous infecting phage results in a self-inflicted abortive infection
    David I Friedman
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Microbiol 82:567-77. 2011
    ..Exclusion of HK97 appears to be specific since other phages tested, λ, φ80, H-19B, λ-P22dis and T4rII, were not excluded. Infection of the 933W lysogen with a non-excluded phage fails to induce Stk-determined phosphorylation...
  3. ncbi request reprint Transcription antitermination: the lambda paradigm updated
    D I Friedman
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 620, USA
    Mol Microbiol 18:191-200. 1995
    ..iv) A host-encoded inhibitor of N antitermination appears to act through the NUT site as well as with the alpha subunit of RNA polymerase, and is antagonized by NusB protein...
  4. ncbi request reprint Bacteriophage lambda: alive and well and still doing its thing
    D I Friedman
    Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor Michigan, Ann Arbor, Michigan 48109 0620, USA
    Curr Opin Microbiol 4:201-7. 2001
    ..Recent work suggests that the phage regulatory network may be a significant contributor to toxin production and release by these pathogenic E. coli...
  5. pmc Evidence that the promoter can influence assembly of antitermination complexes at downstream RNA sites
    Ying Zhou
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
    J Bacteriol 188:2222-32. 2006
    ..By blocking antitermination and thus expression of late functions, the effect of increased transcription through nut sites could be physiologically important in maintaining proper regulation of gene expression early in phage development...
  6. pmc The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression
    Jessica S Tyler
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48103, USA
    J Bacteriol 186:7670-9. 2004
    ..This finding provides direct evidence that measurable expression of the stx genes encoded by a 933W prophage requires induction of that prophage with the concomitant initiation of phage gene expression...
  7. pmc Pathogenesis of renal disease due to enterohemorrhagic Escherichia coli in germ-free mice
    Kathryn A Eaton
    Unit for Laboratory Animal Medicine, 018 Animal Research Facility, University of Michigan, Ann Arbor, MI 48109 0614, USA
    Infect Immun 76:3054-63. 2008
    ..The disease in mice was Stx2 and bacterial strain dependent. This animal model should be a useful tool for studying the pathogenesis of renal disease secondary to EHEC infection...
  8. ncbi request reprint Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein
    Ying Zhou
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
    J Mol Biol 318:1175-88. 2002
    ..These studies provide direct evidence that the both KH as well as the S1 RNA binding domains are important for NusA action in support of bacterial viability as well as transcription antitermination mediated by the lambda N protein...
  9. ncbi request reprint Characterizing spontaneous induction of Stx encoding phages using a selectable reporter system
    Jonathan Livny
    Program in Cell and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Microbiol 51:1691-704. 2004
    ..iv) Only a small fraction of the lysogens in a culture spontaneously induce and when the lysogen carries two lambdoid prophages with different repressor/operators, 933W and H-19B, usually both prophages in the same cell are induced...
  10. ncbi request reprint Analyzing transcription antitermination in lambdoid phages encoding toxin genes
    Melody N Neely
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Methods Enzymol 371:418-38. 2003
  11. ncbi request reprint The biological roles of trans-translation
    Jeffrey H Withey
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Curr Opin Microbiol 5:154-9. 2002
    ..However, tmRNA-targeted proteolysis may be important for fine-tuning expression of certain genes by altering the concentration of regulatory proteins. Here, we review recent literature that addresses the biological functions of tmRNA...
  12. pmc Characterization of a eukaryotic-like tyrosine protein kinase expressed by the Shiga toxin-encoding bacteriophage 933W
    Jessica S Tyler
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109 0620, USA
    J Bacteriol 186:3472-9. 2004
    ..The stk gene was identified in prophages obtained from independently isolated Stx-producing Escherichia coli clinical isolates, suggesting that selective pressure has maintained the stk gene in these pathogenic bacteria...
  13. ncbi request reprint A salvage pathway for protein structures: tmRNA and trans-translation
    Jeffrey H Withey
    Unit for Lab Animal Medicine, University of Michigan Medical School, 104 ARF, Ann Arbor, Michigan 48109 0614, USA
    Annu Rev Microbiol 57:101-23. 2003
    ..Both the release of stalled ribosomes and the subsequent degradation of tagged proteins are important consequences of trans-translation...
  14. pmc Requirement for NusG for transcription antitermination in vivo by the lambda N protein
    Ying Zhou
    Department of Microbiology and Immunology, The University of Michigan, Medical School, Ann Arbor, Michigan 48109 0620, USA
    J Bacteriol 184:3416-8. 2002
    ..Earlier work suggested that NusG was not required for N activity in vivo. Here we present evidence that NusG also stimulates N-mediated transcription antitermination in intact cells...
  15. ncbi request reprint Phage regulatory circuits and virulence gene expression
    Matthew K Waldor
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, MA 02111, USA
    Curr Opin Microbiol 8:459-65. 2005
    ..In the CTXphi prophage, binding of LexA, the bacterial regulator of SOS, in addition to binding of the repressor is involved in repression; the repressor has only limited control over CTX production and has no influence on its release...
  16. ncbi request reprint Bacteriophage control of Shiga toxin 1 production and release by Escherichia coli
    Patrick L Wagner
    Howard Hughes Medical Institute and Division of Geographic Medicine and Infectious Diseases, New England Medical Center and Tufts University School of Medicine, Boston, MA 02111, USA
    Mol Microbiol 44:957-70. 2002
    ..By amplifying stx(1)AB copy number, regulating stx(1)AB transcription and allowing for Stx1 release, the biology of the Stx-encoding phages contributes greatly to the production of Stx, the principal virulence factor of STEC...

Research Grants10

  1. Role of phage in expression & transmission of STX genes
    David Friedman; Fiscal Year: 2007
    ..Moreover, this information should also contribute in a general way to the understanding of how interactions of bacteria and their environment influence the activity of their prophages, many of which encode virulence factors. ..
  2. PHAGE AND EXPRESSION AND TRANSMISSION OF SLT GENES
    David Friedman; Fiscal Year: 2003
    ..Information obtained from the in vitro studies will eventually be used to facilitate studies on the role of these phage factors in the expression of Stx in vivo. ..