C W Frevert

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Mechanical ventilation interacts with endotoxemia to induce extrapulmonary organ dysfunction
    D Shane O'Mahony
    Division of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Crit Care 10:R136. 2006
  2. ncbi Binding of interleukin-8 to heparan sulfate and chondroitin sulfate in lung tissue
    Charles W Frevert
    Medical Research Service, VA Puget Sound Medical Center, Seattle and Department of Medicine, University of Washington, Seattle, USA
    Am J Respir Cell Mol Biol 28:464-72. 2003
  3. ncbi Tissue-specific mechanisms control the retention of IL-8 in lungs and skin
    Charles W Frevert
    Medical Research Service and Division of Pulmonary Critical Care Medicine, Department of Medicine, Seattle Department of Veterans Affairs Medical Center, Seattle, WA 98108, USA
    J Immunol 168:3550-6. 2002
  4. ncbi Effect of CD14 blockade in rabbits with Escherichia coli pneumonia and sepsis
    C W Frevert
    Medical Research Service of the Seattle Department of Veterans Affairs Medical Center, Division of Pulmonary Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98101, USA
    J Immunol 164:5439-45. 2000
  5. ncbi The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis
    C W Frevert
    Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA
    Exp Lung Res 24:745-58. 1998
  6. ncbi Rapid fluorescence-based measurement of neutrophil migration in vitro
    C W Frevert
    Medical Research Service of the Seattle VA Medical Center, Department of Medicine, University of Washington School of Medicine, 98108, USA
    J Immunol Methods 213:41-52. 1998
  7. ncbi Recombinant human Fas ligand induces alveolar epithelial cell apoptosis and lung injury in rabbits
    G Matute-Bello
    Medical Research Service, Seattle Veterans Affairs Medical Center, Seattle 98108-1597, USA
    Am J Physiol Lung Cell Mol Physiol 281:L328-35. 2001
  8. doi Effect of Toll-like receptor 4 blockade on pulmonary inflammation caused by mechanical ventilation and bacterial endotoxin
    Lincoln S Smith
    Division of Pediatric Critical Care, Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA
    Exp Lung Res 34:225-43. 2008
  9. ncbi Duffy antigen facilitates movement of chemokine across the endothelium in vitro and promotes neutrophil transmigration in vitro and in vivo
    Janet S Lee
    Medical Research Service, Veterans Affairs Puget Sound Health Care System and Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98108, USA
    J Immunol 170:5244-51. 2003
  10. ncbi Defective pulmonary recruitment of neutrophils in a rat model of endotoxemia
    C W Frevert
    Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115
    Am J Respir Cell Mol Biol 11:716-23. 1994

Collaborators

Detail Information

Publications40

  1. pmc Mechanical ventilation interacts with endotoxemia to induce extrapulmonary organ dysfunction
    D Shane O'Mahony
    Division of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Crit Care 10:R136. 2006
    ....
  2. ncbi Binding of interleukin-8 to heparan sulfate and chondroitin sulfate in lung tissue
    Charles W Frevert
    Medical Research Service, VA Puget Sound Medical Center, Seattle and Department of Medicine, University of Washington, Seattle, USA
    Am J Respir Cell Mol Biol 28:464-72. 2003
    ..These findings suggest that IL-8-glycosaminoglycan interactions determine the location where IL-8 binds in lung tissue and provides a site for the dimerization of IL-8, which increases the local concentration of IL-8 in the lungs...
  3. ncbi Tissue-specific mechanisms control the retention of IL-8 in lungs and skin
    Charles W Frevert
    Medical Research Service and Division of Pulmonary Critical Care Medicine, Department of Medicine, Seattle Department of Veterans Affairs Medical Center, Seattle, WA 98108, USA
    J Immunol 168:3550-6. 2002
    ..These observations show that the mechanisms that control the retention of IL-8 in tissue include neutrophil migration and dimerization, and that the importance of these varies in different tissues...
  4. ncbi Effect of CD14 blockade in rabbits with Escherichia coli pneumonia and sepsis
    C W Frevert
    Medical Research Service of the Seattle Department of Veterans Affairs Medical Center, Division of Pulmonary Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98101, USA
    J Immunol 164:5439-45. 2000
    ..Blockade of CD14 prevents the deleterious systemic responses that occur in sepsis; however, other measures are necessary to control bacterial proliferation at the primary site of infection...
  5. ncbi The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis
    C W Frevert
    Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA
    Exp Lung Res 24:745-58. 1998
    ..06). Thus, we found that the administration of endotoxin to rats did not impair in vivo AM phagocytic function. In fact, our findings suggest that the i.v. administration of LPS may increase AM phagocytosis of 198Au...
  6. ncbi Rapid fluorescence-based measurement of neutrophil migration in vitro
    C W Frevert
    Medical Research Service of the Seattle VA Medical Center, Department of Medicine, University of Washington School of Medicine, 98108, USA
    J Immunol Methods 213:41-52. 1998
    ..This new assay is a significant advance in the measurement of leukocyte migration in vitro...
  7. ncbi Recombinant human Fas ligand induces alveolar epithelial cell apoptosis and lung injury in rabbits
    G Matute-Bello
    Medical Research Service, Seattle Veterans Affairs Medical Center, Seattle 98108-1597, USA
    Am J Physiol Lung Cell Mol Physiol 281:L328-35. 2001
    ..We conclude that the alveolar epithelium of normal rabbits expresses Fas and that sFasL induces lung injury and inflammation in rabbits...
  8. doi Effect of Toll-like receptor 4 blockade on pulmonary inflammation caused by mechanical ventilation and bacterial endotoxin
    Lincoln S Smith
    Division of Pediatric Critical Care, Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA
    Exp Lung Res 34:225-43. 2008
    ..In contrast, blockade of CD14 did not significantly decrease the number of neutrophils or the amount of CXCL8. These data show that TLR4 blockade reduces pulmonary inflammation caused by the combination of LPS and Mechanical ventilation...
  9. ncbi Duffy antigen facilitates movement of chemokine across the endothelium in vitro and promotes neutrophil transmigration in vitro and in vivo
    Janet S Lee
    Medical Research Service, Veterans Affairs Puget Sound Health Care System and Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98108, USA
    J Immunol 170:5244-51. 2003
    ..We conclude that Duffy Ag has a role in enhancing leukocyte recruitment to sites of inflammation by facilitating movement of chemokines across the endothelium...
  10. ncbi Defective pulmonary recruitment of neutrophils in a rat model of endotoxemia
    C W Frevert
    Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115
    Am J Respir Cell Mol Biol 11:716-23. 1994
    ..60% of control group, n = 3), and no change in LFA-1 when normal PMNs were incubated with plasma from rats pretreated with IV LPS (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)..
  11. pmc Fas/Fas ligand system mediates epithelial injury, but not pulmonary host defenses, in response to inhaled bacteria
    G Matute-Bello
    Medical Research Service of the VA Puget Sound Health Care System, Department of Medicine, University of Washington School of Medicine, Seattle, Washington, USA
    Infect Immun 69:5768-76. 2001
    ..The Fas/FasL system did not have a major role in the clearance of aerosolized bacteria from the lungs at the bacterial doses tested...
  12. ncbi Septic shock and acute lung injury in rabbits with peritonitis: failure of the neutrophil response to localized infection
    G Matute-Bello
    Medical Research Service of the Veterans Affairs/Puget Sound Health Care System, and Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98108-1597, USA
    Am J Respir Crit Care Med 163:234-43. 2001
    ..Bacteremia alone is not sufficient to cause lung injury. Lung injury occurs only in the setting of a severe systemic inflammatory response and an inadequate leukocyte response at the primary site of infection...
  13. pmc Effects of age on the synergistic interactions between lipopolysaccharide and mechanical ventilation in mice
    Lincoln S Smith
    Division of Pediatric Critical Care Medicine, Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA
    Am J Respir Cell Mol Biol 43:475-86. 2010
    ..These data suggest that the synergistic inflammatory and injury responses to inhaled LPS combined with MV are acquired with age as a result of coordinated changes in gene expression of inflammatory, apoptotic, and TGF-β pathways...
  14. ncbi High and low inflammatory response phenotypes in 101 normal human subjects
    William Y Park
    Medical Research of the Seattle VA Medical Center and the Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA
    Chest 121:87S-88S. 2002
  15. pmc Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice
    R A Bem
    Research Service of the Veterans Affairs Puget Sound Health Care System, University of Washington, Seattle, Washington, USA
    Am J Physiol Lung Cell Mol Physiol 295:L314-25. 2008
    ..These results suggest that the lung inflammatory response to Fas activation is not primarily dependent on resident alveolar macrophages and may instead depend on cytokine release by alveolar epithelial cells...
  16. pmc Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice
    Kyoung Hee Kim
    Section of Pulmonary and Critical Care Medicine, Fred Hutchinson Cancer Research Center, University of Washington School of Medicine, 1100 Fairview Avenue N, Seattle, WA 98109 1024, USA
    Am J Respir Cell Mol Biol 33:271-9. 2005
    ..Our results provide direct evidence that TIMP-1 contributes significantly to the regulation of acute lung injury, functioning to limit inflammation and lung permeability...
  17. ncbi TLR-4 pathway mediates the inflammatory response but not bacterial elimination in E. coli pneumonia
    Janet S Lee
    Veterans Affairs Puget Sound Health Care System and the Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle, Washington 98108, USA
    Am J Physiol Lung Cell Mol Physiol 289:L731-8. 2005
    ..Our findings indicate that the inflammatory response to inhaled E. coli is TLR-4 dependent, but bacterial elimination depends on other factors in addition to TLR-4...
  18. ncbi Fas-mediated acute lung injury requires fas expression on nonmyeloid cells of the lung
    Gustavo Matute-Bello
    Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 175:4069-75. 2005
    ..These findings suggest that the alveolar epithelium is the primary target of Fas-mediated acute lung injury, and demonstrate that apoptotic processes may be associated with neutrophilic inflammation...
  19. ncbi Rabbit models of pneumonia, peritoneal sepsis, and lung injury
    C W Frevert
    Seattle Department of Veterans Affairs Medical Center, WA, USA
    Methods Mol Biol 138:319-30. 2000
    ..g., recombinant proteins and MAbs). A disadvantage of the rabbit model is that there are fewer reagents (e.g., recombinant cytokines and MAbs) available for the study of inflammation in rabbits when compared to mice...
  20. ncbi Gene expression of Toll-like receptor-2, Toll-like receptor-4, and MD2 is differentially regulated in rabbits with Escherichia coli pneumonia
    Osamu Kajikawa
    Pulmonary Research Laboratories at the Seattle Department of Veterans Affairs Medical Center, University of Washington School of Medicine, 151L 1660 South Columbian Way, Seattle, WA 98108, USA
    Gene 344:193-202. 2005
    ..In contrast, the increased gene expression of TLR2, TLR4, and MD2 in organs distant to the primary site of infection may contribute to the deleterious systemic inflammatory response observed in patients with sepsis...
  21. pmc The Duffy antigen modifies systemic and local tissue chemokine responses following lipopolysaccharide stimulation
    Janet S Lee
    Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA
    J Immunol 177:8086-94. 2006
    ..These data indicate that dfy alters soluble chemokine concentrations in blood and local tissue compartments and enhances systemic bioavailability of chemokines produced during local tissue inflammation...
  22. pmc Differential response of human lung epithelial cells to fas-induced apoptosis
    Morio Nakamura
    Department of Medicine, University of Washington School of Medicine, Seattle, Washington, USA
    Am J Pathol 164:1949-58. 2004
    ..These findings are relevant for understanding the role of the Fas/FasL system in acute lung injury, in which epithelial damage occurs primarily in distal airway and alveolar epithelium, whereas sFasL is present throughout the airspaces...
  23. pmc Animal models of acute lung injury
    Gustavo Matute-Bello
    Medical Research Service of the Veterans Affairs Puget Sound Health Care System, 815 Mercer St, Seattle, WA 98109, USA
    Am J Physiol Lung Cell Mol Physiol 295:L379-99. 2008
    ..The present review will help guide investigators in the design and interpretation of animal studies of acute lung injury...
  24. doi Kinetics of chemokine-glycosaminoglycan interactions control neutrophil migration into the airspaces of the lungs
    Yoshi Tanino
    Veterans Affairs Puget Sound Medical Center, Seattle, WA 98108, USA
    J Immunol 184:2677-85. 2010
    ..Thus, different types of chemokine gradients may form during an inflammatory response. This suggests a new model, whereby GAGs control the spatiotemporal formation of chemokine gradients and neutrophil migration in tissue...
  25. pmc Essential role of MMP-12 in Fas-induced lung fibrosis
    Gustavo Matute-Bello
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington, South Lake Union Campus, 815 Mercer Street, Seattle, WA 98109, USA
    Am J Respir Cell Mol Biol 37:210-21. 2007
    ..We speculate that MMP-12 activity is required for activation of the profibrotic genes egr1 and cyr61...
  26. doi Inhalation of Burkholderia thailandensis results in lethal necrotizing pneumonia in mice: a surrogate model for pneumonic melioidosis
    T Eoin West
    Department of Medicine, University of Washington, Box 359640, 325 9th Avenue, Seattle, WA 98104 2499, USA
    Trans R Soc Trop Med Hyg 102:S119-26. 2008
    ..These findings demonstrate that airborne B. thailandensis infection in mice provides an accessible surrogate model of melioidosis...
  27. pmc Innate immunity in the lungs
    Thomas R Martin
    Pulmonary Research Laboratories, VA Puget Sound Health Care System, 151L, 1660 South Columbian Way, Seattle, WA 98108, USA
    Proc Am Thorac Soc 2:403-11. 2005
    ..New strategies to modify innate immune responses could be useful in limiting the adverse consequences of some inflammatory reactions in the lungs...
  28. ncbi Blockade of the Fas/FasL system improves pneumococcal clearance from the lungs without preventing dissemination of bacteria to the spleen
    Gustavo Matute-Bello
    Medical Research Service of the Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108 1597, USA
    J Infect Dis 191:596-606. 2005
    ..FasL is inhibited by decoy receptor-3 (DcR3), a naturally occurring decoy receptor. We determined the effects of systemic blockade of the Fas/FasL system by a DcR3 analog (DcR3-a) in mice with pneumococcal pneumonia...
  29. ncbi Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages
    David R Park
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98104, USA
    J Immunol 170:6209-16. 2003
    ..Rather than contributing to the resolution of inflammation, Fas ligation on circulating monocytes and tissue macrophages may induce proinflammatory cytokine responses that can initiate acute inflammatory responses and tissue injury...
  30. ncbi Expression of the integrin subunit alpha8 in murine lung development
    Teresa E Wagner
    University of Washington, Division of Pulmonary and Critical Care Medicine, Harborview Medical Center, Seattle, Washington, USA
    J Histochem Cytochem 51:1307-15. 2003
    ..Co-localization of alpha 8 with fibronectin suggests a role in branching morphogenesis. Furthermore, alpha 8 may participate in secondary septation by modulating signals from the extracellular matrix to alveolar myofibroblasts...
  31. ncbi Mechanical ventilation with moderate tidal volumes synergistically increases lung cytokine response to systemic endotoxin
    William A Altemeier
    Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle 98195 6522, USA
    Am J Physiol Lung Cell Mol Physiol 287:L533-42. 2004
    ..We conclude that moderate volume ventilation strategies augment the innate immune response to bacterial products in the lung and may play a role in the development of acute lung injury in patients with sepsis...
  32. ncbi Measurement of cell migration in response to an evolving radial chemokine gradient triggered by a microvalve
    Charles W Frevert
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Lab Chip 6:849-56. 2006
    ..The microVCD can be easily adapted to study the migratory behavior of cultured cells other than neutrophils...
  33. ncbi Enhanced expression of Duffy antigen in the lungs during suppurative pneumonia
    Janet S Lee
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle, USA
    J Histochem Cytochem 51:159-66. 2003
    ....
  34. ncbi Differential regulation of membrane CD14 expression and endotoxin-tolerance in alveolar macrophages
    Shu Min Lin
    Pulmonary Research Laboratories, VA Puget Sound Medical Center, 1660 S Columbian Way, 151L, Seattle, WA 98108, USA
    Am J Respir Cell Mol Biol 31:162-70. 2004
    ..coli but not LPS. These studies show that distinct mechanisms regulate the expression of mCD14 and the induction of endotoxin tolerance in AM, and suggest that AM function is impaired at sites of bacterial infection...
  35. doi Proteoglycans: key regulators of pulmonary inflammation and the innate immune response to lung infection
    Sean Gill
    Center for Lung Biology, University of Washington School of Medicine at South Lake Union, Seattle, Washington, USA
    Anat Rec (Hoboken) 293:968-81. 2010
    ..Clarification of the mechanisms whereby proteoglycans modulate the pulmonary inflammatory response will most likely lead to new therapeutic approaches to inflammatory lung disease and lung infection...
  36. doi Systemic inflammatory response syndrome in nonhuman primates culminating in multiple organ failure, acute lung injury, and disseminated intravascular coagulation
    Renee R Hukkanen
    University of Washington, Department of Comparative Medicine, Seattle, Washington 98195 7190, USA
    Toxicol Pathol 37:799-804. 2009
    ..Experimental manipulation of NHP models may be complicated by SIRS and accompanying rapid clinical decompensation. Such adverse events may compromise toxicological studies and should be avoided when possible...
  37. ncbi Optimal timing to repopulation of resident alveolar macrophages with donor cells following total body irradiation and bone marrow transplantation in mice
    Gustavo Matute-Bello
    Pulmonary and Critical Care Division, Department of Medicine, University of Washington, Seattle, WA 98108, USA
    J Immunol Methods 292:25-34. 2004
    ..This study provides useful information regarding the time of reconstitution with donor alveolar macrophages in the pulmonary airspaces of recipient mice following marrow transplantation...
  38. ncbi Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits
    Kiyoshi Moriyama
    Department of Anesthesiology, School of Medicine, Keio University, Tokyo, Japan
    Am J Physiol Lung Cell Mol Physiol 286:L1114-21. 2004
    ..These results suggest that mechanical stress caused by a large V(t) sensitizes the lungs to endotoxin, a phenomenon that may occur partially via the upregulation of CD14...
  39. pmc Spatial and temporal heterogeneity of ventilator-associated lung injury after surfactant depletion
    Cynthia M Otto
    Departmrnt of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA, USA
    J Appl Physiol 104:1485-94. 2008
    ..Temporal and regional heterogeneity of lung injury were substantial. In this surfactant depletion model of acute lung injury, cyclical recruitment produced more injury than stretch...
  40. pmc Anti-KC autoantibody:KC complexes cause severe lung inflammation in mice via IgG receptors
    Agnieszka Krupa
    Department of Biochemistry, University of Texas Health Center, Tyler, Texas 75708 3154, USA
    Am J Respir Cell Mol Biol 37:532-43. 2007
    ..Our results suggest that immune complexes containing autoantibodies contribute to development of lung inflammation in LPS-treated mice...