Affiliation: University of Colorado Health Sciences Center
- Will embryonic stem cells be a useful source of dopamine neurons for transplant into patients with Parkinson's disease?Curt R Freed
Division of Clinical Pharmacology and Toxicology, University of Colorado School of Medicine, C237, 4200 East Ninth Avenue, Denver, CO 80220, USA
Proc Natl Acad Sci U S A 99:1755-7. 2002
- Do patients with Parkinson's disease benefit from embryonic dopamine cell transplantation?Curt R Freed
Departments of Medicine, Neurology, Psychiatry, and Neurosurgery Box, University of Colorado School of Medicine, 4200 East Ninth Avenue, C237, Denver, CO 80220, USA
J Neurol 250:III44-6. 2003..Neurotransplantation should be tried earlier in the clinical course of Parkinson's to see if earlier intervention can prevent progression of the disease, particularly the dyskinetic responses seen after longterm L-dopa treatment...
- Does personality change as a result of fetal tissue transplantation in the brain?Cynthia McRae
College of Education, University of Denver, Denver, CO 80208, USA
J Neurol 250:282-6. 2003..Results indicate that personality, as measured by the NEO-FFI, basically remained stable during the two-year follow-up period of this study. In this case, no change is regarded as a positive outcome...
- Overexpression of human alpha-synuclein causes dopamine neuron death in primary human mesencephalic cultureWenbo Zhou
Division of Clinical Pharmacology, Department of Medicine, and the Neuroscience Program, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
Brain Res 926:42-50. 2002..These results show that overexpression of human alpha-synuclein, particularly the mutant form, can cause human DA neuron death, suggesting that alpha-synuclein may have a primary role in the pathogenesis of PD...
- DJ-1 up-regulates glutathione synthesis during oxidative stress and inhibits A53T alpha-synuclein toxicityWenbo Zhou
Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Biol Chem 280:43150-8. 2005..We conclude that DJ-1 has multiple specific mechanisms for protecting dopamine neurons from cell death...
- Characterization of the Bex gene family in humans, mice, and ratsEnrique Alvarez
Medical Scientist Training Program, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Gene 357:18-28. 2005..We conclude that the Bex gene family members are highly homologous but differ in their expression patterns, subcellular localization, and degradation by the proteasome...
- Generation of tyrosine hydroxylase positive neurons from human embryonic stem cells after coculture with cellular substrates and exposure to GDNFKimberley A Buytaert-Hoefen
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262, USA
Stem Cells 22:669-74. 2004..05). We conclude that substrates of striatal astrocytes and PA6 cells can promote differentiation of human embryonic stem cells to a TH-positive phenotype and that GDNF can increase the number of cells expressing that phenotype...
- Reaction time and movement time after embryonic cell implantation in Parkinson diseasePaul H Gordon
Department of Neurology and Clinical Motor Physiology Laboratory, Columbia Presbyterian Medical Center, New York, NY 10032, USA
Arch Neurol 61:858-61. 2004..Reaction time (RT) and movement time (MT) analysis, validated quantitative measures of premovement neural processing and motor execution, can be used as objective physiological markers of motor performance in PD...
- Preoperative response to levodopa is the best predictor of transplant outcomeCurt R Freed
Ann Neurol 55:896; author reply 896-7. 2004
- Effects of perceived treatment on quality of life and medical outcomes in a double-blind placebo surgery trialCynthia McRae
College of Education, University of Denver, Denver, CO 80208, USA
Arch Gen Psychiatry 61:412-20. 2004..This portion of the study investigated the quality of life (QOL) of participants during the 1 year of double-blind follow-up...
- Neural transplantation for the treatment of Parkinson's diseaseAnders Bjorklund
Wallenberg Neuroscience Center, Section of Neurobiology, Lund University, Sweden
Lancet Neurol 2:437-45. 2003
- Distinct patterns of gene expression induced by viral oncogenes in human embryonic brain cellsPiruz Nahreini
Department of Radiology, Center for Vitamins and Cancer Research, School of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Cell Mol Neurobiol 23:27-42. 2003..7. This suggests that the mechanisms for inducing neuronal differentiation are still intact in HEB-E6E7 and HEB-LTA cells. More importantly, differentiation signals can override the effects of viral oncogenes in HEB cells...
- Xenografts of MHC-deficient mouse embryonic mesencephalon improve behavioral recovery in hemiparkinsonian ratsLone M Veng
Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA
Cell Transplant 11:5-16. 2002..Our results show that MHC class II-deficient xenografts survive transplantation for at least 6 weeks in the absence of immunosuppression, reduce rotational asymmetry, and provoke lesser immune reaction than wt grafts...
- Transgenic mice overexpressing tyrosine-to-cysteine mutant human alpha-synuclein: a progressive neurodegenerative model of diffuse Lewy body diseaseWenbo Zhou
Division of Clinical Pharmacology and Toxicology, Department of Medicine, School of Medicine, University of Colorado Denver, 4200 E 9th Avenue, Denver, CO 80262, USA
J Biol Chem 283:9863-70. 2008....
- Tyrosine-to-cysteine modification of human alpha-synuclein enhances protein aggregation and cellular toxicityWenbo Zhou
Division of Clinical Pharmacology, Department of Medicine, and the Neuroscience Program, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Biol Chem 279:10128-35. 2004..We conclude that cysteine substitution at critical positions in the alpha-synuclein molecule can increase dimer formation and accelerate protein aggregation and cellular toxicity of alpha-synuclein...
- The pesticide rotenone induces caspase-3-mediated apoptosis in ventral mesencephalic dopaminergic neuronsFerogh A Ahmadi
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Neurochem 87:914-21. 2003..Understanding the mechanism of rotenone-induced apoptosis in dopamine neurons may contribute to the development of new neuroprotective strategies against Parkinson's disease...
- Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell lineBirgit Schilling
Buck Institute for Age Research, Novato, CA 94945, USA
Mol Cell Proteomics 4:84-96. 2005....
- Placebo response in Parkinson's disease: comparisons among 11 trials covering medical and surgical interventionsChristopher G Goetz
Department of Neurological Sciences, Rush University Medical Center, Chicago, Illinois 60612, USA
Mov Disord 23:690-9. 2008..Recognition of factors that impact placebo response rates should be incorporated into individual study designs for PD clinical trials...
- EMBRYONIC DOPAMINE CELL IMPLANTS FOR PARKINSONISMCurt Freed; Fiscal Year: 2005..Brains will be stained with H+E and immunostained for tyrosine hydroxylase and B and T cell markers. Stereologically corrected counts of TH-positive neurons will be determined. ..
- EMBRYONIC DOPAMINE CELL IMPLANTS FOR PARKINSONISMCurt Freed; Fiscal Year: 1999..In the long term, we predict that implant patients will show progressive improvement for several years after transplant. Our patient cohort is uniquely able to define the early and long term outcome of neurotransplantation. ..