RENNY THEODORE FRANCESCHI

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Differentiation-dependent association of phosphorylated extracellular signal-regulated kinase with the chromatin of osteoblast-related genes
    Yan Li
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
    J Bone Miner Res 25:154-63. 2010
  2. pmc Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation
    Hernan Roca
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
    Nucleic Acids Res 36:1723-30. 2008
  3. pmc Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development
    Chunxi Ge
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
    J Cell Biol 176:709-18. 2007
  4. ncbi request reprint Gene therapy for bone formation: in vitro and in vivo osteogenic activity of an adenovirus expressing BMP7
    R T Franceschi
    Departments of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Cell Biochem 78:476-86. 2000
  5. pmc Transcriptional regulation of osteoblasts
    Renny T Franceschi
    School of Dentistry, University of Michigan, Ann Arbor, Mich 48109 1078, USA
    Cells Tissues Organs 189:144-52. 2009
  6. ncbi request reprint Transcriptional regulation of osteoblasts
    Renny T Franceschi
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109 1078, USA
    Ann N Y Acad Sci 1116:196-207. 2007
  7. ncbi request reprint Biological approaches to bone regeneration by gene therapy
    R T Franceschi
    University of Michigan School of Dentistry, 1011 N University Ave, Ann Arbor, MI 48109 1078, USA
    J Dent Res 84:1093-103. 2005
  8. pmc Gene therapy approaches for bone regeneration
    Renny T Franceschi
    The Center for Craniofacial Regeneration, University of Michigan School of Dentistry, Ann Arbor, Mich, USA
    Cells Tissues Organs 176:95-108. 2004
  9. ncbi request reprint Functional cooperativity between osteoblast transcription factors: evidence for the importance of subnuclear macromolecular complexes?
    R T Franceschi
    Department of Periodontics Prevention Geriatrics, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109 1078, USA
    Calcif Tissue Int 72:638-42. 2003
  10. pmc Multiple signaling pathways converge on the Cbfa1/Runx2 transcription factor to regulate osteoblast differentiation
    Renny T Franceschi
    Department of Periodontics Prevention Geriatrics, School of Medicine, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    Connect Tissue Res 44:109-16. 2003

Research Grants

  1. COMBINATORIAL GENE THERAPY FOR BONE REGENERATION
    Renny Franceschi; Fiscal Year: 2007
  2. ECM REGULATION OF OSTEOBLAST GENE EXPRESSION
    Renny Franceschi; Fiscal Year: 2005
  3. BONE SIALOPROTEIN--EXPRESSION AND ROLE IN MINERALIZATION
    Renny Franceschi; Fiscal Year: 2000
  4. BONE SIALOPROTEIN: EXPRESSION & ROLE IN MINERALIZATION
    Renny Franceschi; Fiscal Year: 2004
  5. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010
  6. GENE THERAPY APPROACH FOR ENGINEERING CRANIOFACIAL BONE
    Renny Franceschi; Fiscal Year: 2004
  7. COMBINATORIAL GENE THERAPY FOR BONE REGENERATION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010
  8. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    Renny Franceschi; Fiscal Year: 2007
  9. ROLES OF ASCORBIC ACID IN OSTEOBLAST DIFFERENTIATION
    Renny Franceschi; Fiscal Year: 1999
  10. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010

Collaborators

Detail Information

Publications36

  1. pmc Differentiation-dependent association of phosphorylated extracellular signal-regulated kinase with the chromatin of osteoblast-related genes
    Yan Li
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
    J Bone Miner Res 25:154-63. 2010
    ....
  2. pmc Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation
    Hernan Roca
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
    Nucleic Acids Res 36:1723-30. 2008
    ....
  3. pmc Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development
    Chunxi Ge
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
    J Cell Biol 176:709-18. 2007
    ..This work establishes an important in vivo function for the ERK-MAPK pathway in bone that involves stimulation of RUNX2 phosphorylation and transcriptional activity...
  4. ncbi request reprint Gene therapy for bone formation: in vitro and in vivo osteogenic activity of an adenovirus expressing BMP7
    R T Franceschi
    Departments of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Cell Biochem 78:476-86. 2000
    ....
  5. pmc Transcriptional regulation of osteoblasts
    Renny T Franceschi
    School of Dentistry, University of Michigan, Ann Arbor, Mich 48109 1078, USA
    Cells Tissues Organs 189:144-52. 2009
    ..These studies allow us to begin understanding the complex mechanisms necessary to fine-tune bone formation as mesenchymal progenitors progress down the osteoblast lineage...
  6. ncbi request reprint Transcriptional regulation of osteoblasts
    Renny T Franceschi
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109 1078, USA
    Ann N Y Acad Sci 1116:196-207. 2007
    ..These studies allow us to begin understanding the complex mechanisms necessary to fine-tune bone formation in response to extracellular stimuli including ECM interactions, mechanical loads, and hormonal stimulation...
  7. ncbi request reprint Biological approaches to bone regeneration by gene therapy
    R T Franceschi
    University of Michigan School of Dentistry, 1011 N University Ave, Ann Arbor, MI 48109 1078, USA
    J Dent Res 84:1093-103. 2005
    ....
  8. pmc Gene therapy approaches for bone regeneration
    Renny T Franceschi
    The Center for Craniofacial Regeneration, University of Michigan School of Dentistry, Ann Arbor, Mich, USA
    Cells Tissues Organs 176:95-108. 2004
    ..Using these types of approaches, it should be possible to mimic natural processes of bone development and fracture repair and, in so doing, be able to precisely control both the amount and type of bone regenerated...
  9. ncbi request reprint Functional cooperativity between osteoblast transcription factors: evidence for the importance of subnuclear macromolecular complexes?
    R T Franceschi
    Department of Periodontics Prevention Geriatrics, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109 1078, USA
    Calcif Tissue Int 72:638-42. 2003
    ..The possible relationship between these complexes and the subnuclear localization of the osteoblast transcriptional apparatus will also be discussed...
  10. pmc Multiple signaling pathways converge on the Cbfa1/Runx2 transcription factor to regulate osteoblast differentiation
    Renny T Franceschi
    Department of Periodontics Prevention Geriatrics, School of Medicine, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    Connect Tissue Res 44:109-16. 2003
    ..These findings suggest that Runx2 plays a central role in coordinating multiple signals involved in osteoblast differentiation...
  11. ncbi request reprint Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways
    Renny T Franceschi
    Department of Periodontics, School of Dentistry, Ann Arbor, Michigan 48109 1078, USA
    J Cell Biochem 88:446-54. 2003
    ..These findings suggest that Runx2 plays a central role in coordinating multiple signals involved in osteoblast differentiation...
  12. ncbi request reprint Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2
    Guozhi Xiao
    Department of Periodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 277:36181-7. 2002
    ..This study is the first demonstration that Runx2 is phosphorylated and activated by FGF-2 via the MAPK pathway and suggests that FGF-2 plays an important role in regulation of Runx2 function and bone formation...
  13. ncbi request reprint Parathyroid hormone induction of the osteocalcin gene. Requirement for an osteoblast-specific element 1 sequence in the promoter and involvement of multiple-signaling pathways
    Di Jiang
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 279:5329-37. 2004
    ..Collectively, these studies establish that actions of PTH on the osteocalcin gene are mediated by multiple signaling pathways and require OSE1 and associated nuclear proteins...
  14. ncbi request reprint Impact of the mitogen-activated protein kinase pathway on parathyroid hormone-related protein actions in osteoblasts
    Chen Chen
    Department of Periodontics Prevention Geriatrics, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 279:29121-9. 2004
    ..These novel findings indicate that the MAPK pathway plays a selective but important role in the actions of PTHrP...
  15. ncbi request reprint Cooperative interactions between RUNX2 and homeodomain protein-binding sites are critical for the osteoblast-specific expression of the bone sialoprotein gene
    Hernan Roca
    Department of Periodontics, Prevention, and Geriatrics and Center for Craniofacial Regeneration, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Biol Chem 280:30845-55. 2005
    ..Taken together, our data show that RUNX2 is a direct regulator of Bsp in osteoblasts and that it functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression...
  16. ncbi request reprint Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells
    Guozhi Xiao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Bone Miner Res 17:101-10. 2002
    ..This work establishes that autocrine BMP production as well as integrin-mediated cell-collagen interactions are both required for osteoblast differentiation, and both these pathways require MAP kinase activity...
  17. pmc In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation
    Shuying Yang
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Bone Miner Res 18:705-15. 2003
    ..These results suggest that Runx2 and BMP2 have distinct, but complementary, roles in osteogenesis and that their combined actions may be necessary for optimal bone formation...
  18. ncbi request reprint Bone morphogenetic protein-transduced human fibroblasts convert to osteoblasts and form bone in vivo
    R Bruce Rutherford
    School of Dentistry, Center for Biorestoration of Oral Health, University of Michigan, 1011 N University, Ann Arbor, MI 48109 1078, USA
    Tissue Eng 8:441-52. 2002
    ..Only the BMP-transduced fibroblasts formed bone within the diffusion chambers in vivo, revealing that BMP transduction induces osteoblastic conversion of these cells...
  19. ncbi request reprint Bone morphogenetic protein 2 induces dental follicle cells to differentiate toward a cementoblast/osteoblast phenotype
    Ming Zhao
    Department of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
    J Bone Miner Res 17:1441-51. 2002
    ..These results suggest that BMP-2 triggers follicle cells to differentiate toward a cementoblast/osteoblast phenotype and that the MAPK pathway is involved...
  20. ncbi request reprint A homeodomain protein binding element in the bone sialoprotein promoter is critical for tissue-specific expression in bone
    Rajaram Gopalakrishnan
    Department of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    Connect Tissue Res 44:154-60. 2003
    ..We therefore hypothesize that the OSHE1 site is involved in both the expression of Bsp in mineralizing tissues and the suppression of transcription in nonmineralizing tissues...
  21. ncbi request reprint Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7
    Ming Zhao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry and Center for Craniofacial Regeneration, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Cell Biochem 95:1-16. 2005
    ..These studies show that dramatic enhancement of osteogenesis can be achieved using gene therapy to express specific combinations of interacting regenerative molecules...
  22. pmc Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell niche
    Younghun Jung
    Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, 1011 North University Avenue, Ann Arbor, Michigan 48109 1078, USA
    Stem Cells 26:2042-51. 2008
    ..Instead, they directly participate in bone formation and niche activities. Disclosure of potential conflicts of interest is found at the end of this article...
  23. ncbi request reprint Gene transfer of the Runx2 transcription factor enhances osteogenic activity of bone marrow stromal cells in vitro and in vivo
    Zhuoran Zhao
    Program in Oral Health Sciences, University of Michigan, 1011 North University Avenue, Ann Arbor, MI 48109, USA
    Mol Ther 12:247-53. 2005
    ..Taken together, these studies indicate that Runx2 gene transfer may be an effective route to enhance the osteogenic potential of MSCs...
  24. doi request reprint Gene delivery by adenoviruses
    Renny T Franceschi
    Department of Periodontics and Oral Medicine, School of Dentistry, and Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI, USA
    Methods Mol Biol 455:137-47. 2008
    ..In this chapter, procedures will be described for adenovirus construction and virus delivery to in vivo sites for measurement of osteogenic activity...
  25. pmc BMP gene delivery for alveolar bone engineering at dental implant defects
    Courtney A Dunn
    Department of Orthodontics and Pediatric Dentistry, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Ther 11:294-9. 2005
    ..In vivo gene therapy of BMP-7 offers potential for alveolar bone engineering applications...
  26. pmc Osteoblast differentiation stage-specific expression of the pyrophosphate-generating enzyme PC-1
    Nan E Hatch
    Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, Mich 48109 1078, USA
    Cells Tissues Organs 189:65-9. 2009
    ..These results also indicate that PC-1 may play multiple and distinct roles in the development of mineralized tissues...
  27. pmc BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype
    Mattabhorn Phimphilai
    Department of Biological Chemistry, University of Michigan School of Medicine, Ann Arbor, Michigan 48109 1078, USA
    J Bone Miner Res 21:637-46. 2006
    ..BMP blocking agents were used to show that RUNX2-dependent osteoblast differentiation and transactivation activity both require BMP signaling and, further, that RUNX2 enhances the responsiveness of cells to BMPs...
  28. ncbi request reprint Cooperative interactions between activating transcription factor 4 and Runx2/Cbfa1 stimulate osteoblast-specific osteocalcin gene expression
    Guozhi Xiao
    Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 280:30689-96. 2005
    ....
  29. pmc FGF2 stimulation of the pyrophosphate-generating enzyme, PC-1, in pre-osteoblast cells is mediated by RUNX2
    Nan E Hatch
    Department of Orthodontics and Pediatric Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Bone Miner Res 24:652-62. 2009
    ..Taken together, our results establish that FGF2 is a specific inducer of PC-1 in pre-osteoblast cells and that FGF2 induces PC-1 expression through a mechanism involving Runx2...
  30. pmc Identification and functional characterization of ERK/MAPK phosphorylation sites in the Runx2 transcription factor
    Chunxi Ge
    Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 284:32533-43. 2009
    ....
  31. ncbi request reprint Use of a stringent dimerizer-regulated gene expression system for controlled BMP2 delivery
    Jeong Tae Koh
    Department of Periodontics and Oral Medicine, School of Dentistry, and Center for Craniofacial Regeneration, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Ther 14:684-91. 2006
    ..This regulated BMP2 expression system will be extremely useful for examining the role of timing and sequence of BMP delivery on bone regeneration...
  32. pmc The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro
    Ying Zhang
    Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109 1078, USA
    Biomaterials 31:3231-6. 2010
    ..These results demonstrate that adenovirus vectors driving the expression of transcription factors can be delivered directly from biomaterials to direct cell differentiation...
  33. pmc Parathyroid hormone increases activating transcription factor 4 expression and activity in osteoblasts: requirement for osteocalcin gene expression
    Shibing Yu
    Division of Hematology Oncology, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
    Endocrinology 149:1960-8. 2008
    ..Collectively, these studies for the first time demonstrate that PTH increases ATF4 expression and activity and that ATF4 is required for PTH induction of Ocn expression in osteoblasts...
  34. ncbi request reprint Regulation of matrix Gla protein by parathyroid hormone in MC3T3-E1 osteoblast-like cells involves protein kinase A and extracellular signal-regulated kinase pathways
    Supaporn Suttamanatwong
    Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, Minnesota 55455, USA
    J Cell Biochem 102:496-505. 2007
    ..Taken together, these results indicate that PTH regulates MGP via both PKA- and ERK-dependent pathways...
  35. ncbi request reprint Role of matrix Gla protein in parathyroid hormone inhibition of osteoblast mineralization
    Rajaram Gopalakrishnan
    Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, Minn 55455, USA
    Cells Tissues Organs 181:166-75. 2005
    ..In addition to MGP, the regulation and possible role of osteopontin, another known regulator of osteoblast mineralization, in PTH-mediated regulation of bone and vascular mineralization is discussed...
  36. ncbi request reprint Bone sialoprotein gene transfer to periodontal ligament cells may not be sufficient to promote mineralization in vitro or in vivo
    Sema S Hakki
    Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey
    J Periodontol 77:167-73. 2006
    ..In this study, we examined whether gene transfer of BSP into periodontal ligament (PDL) cells would result in an increased ability of PDL cells to promote mineralization in vitro and in vivo...

Research Grants36

  1. COMBINATORIAL GENE THERAPY FOR BONE REGENERATION
    Renny Franceschi; Fiscal Year: 2007
    ..Approaches developed will provide proof of principle of the eventual development of new therapies for bone regeneration that mimic natural biological processes by providing temporal control over regenerative events. ..
  2. ECM REGULATION OF OSTEOBLAST GENE EXPRESSION
    Renny Franceschi; Fiscal Year: 2005
    ..They will elucidate a novel mechanism to explain how ECM signals control the activity of a critical osteoblast transcription factor, and show how matrix signals interact with signals generated by BMPs to regulate bone formation. ..
  3. BONE SIALOPROTEIN--EXPRESSION AND ROLE IN MINERALIZATION
    Renny Franceschi; Fiscal Year: 2000
    ....
  4. BONE SIALOPROTEIN: EXPRESSION & ROLE IN MINERALIZATION
    Renny Franceschi; Fiscal Year: 2004
    ..Such knowledge is essential for designing new strategies for bone regeneration to treat trauma and pathological conditions such as osteoporosis and periodontal disease. ..
  5. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010
    ..7. ..
  6. GENE THERAPY APPROACH FOR ENGINEERING CRANIOFACIAL BONE
    Renny Franceschi; Fiscal Year: 2004
    ..These studies will greatly advance our understanding of BMP-dependent bone regeneration and develop a viable gene therapy approach for regenerating bone in the craniofacial and appendicular skeleton. ..
  7. COMBINATORIAL GENE THERAPY FOR BONE REGENERATION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010
    ..Approaches developed will provide proof of principle of the eventual development of new therapies for bone regeneration that mimic natural biological processes by providing temporal control over regenerative events. ..
  8. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    Renny Franceschi; Fiscal Year: 2007
    ....
  9. ROLES OF ASCORBIC ACID IN OSTEOBLAST DIFFERENTIATION
    Renny Franceschi; Fiscal Year: 1999
    ..These studies will enable Dr. Franceschi and his colleagues to gain a better understanding of the mechanisms by which ascorbate affects the programming of bone cell differentiation at the molecular level. ..
  10. MAP KINASE REGULATION OF OSTEOBLAST FUNCTION
    RENNY THEODORE FRANCESCHI; Fiscal Year: 2010
    ..Therapeutic manipulation of the MAPK pathway has the potential to augment bone anabolic therapies such as weight-bearing exercise to prevent or reverse the deleterious effects of osteoporosis. ..