Research Topics
Species | RENNY THEODORE FRANCESCHISummaryAffiliation: University of Michigan Country: USA Publications
Research Grants
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Detail Information
Publications
Gene therapy for bone formation: in vitro and in vivo osteogenic activity of an adenovirus expressing BMP7R T Franceschi
Departments of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
J Cell Biochem 78:476-86. 2000....
Transcriptional regulation of osteoblastsRenny T Franceschi
School of Dentistry, University of Michigan, Ann Arbor, Mich 48109 1078, USA
Cells Tissues Organs 189:144-52. 2009..These studies allow us to begin understanding the complex mechanisms necessary to fine-tune bone formation as mesenchymal progenitors progress down the osteoblast lineage...- Transcriptional regulation of osteoblastsRenny T Franceschi
Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109 1078, USA
Ann N Y Acad Sci 1116:196-207. 2007..These studies allow us to begin understanding the complex mechanisms necessary to fine-tune bone formation in response to extracellular stimuli including ECM interactions, mechanical loads, and hormonal stimulation... - Biological approaches to bone regeneration by gene therapyR T Franceschi
University of Michigan School of Dentistry, 1011 N University Ave, Ann Arbor, MI 48109 1078, USA
J Dent Res 84:1093-103. 2005.... - Gene therapy approaches for bone regenerationRenny T Franceschi
The Center for Craniofacial Regeneration, University of Michigan School of Dentistry, Ann Arbor, Mich, USA
Cells Tissues Organs 176:95-108. 2004..Using these types of approaches, it should be possible to mimic natural processes of bone development and fracture repair and, in so doing, be able to precisely control both the amount and type of bone regenerated... - Functional cooperativity between osteoblast transcription factors: evidence for the importance of subnuclear macromolecular complexes?R T Franceschi
Department of Periodontics Prevention Geriatrics, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109 1078, USA
Calcif Tissue Int 72:638-42. 2003..The possible relationship between these complexes and the subnuclear localization of the osteoblast transcriptional apparatus will also be discussed... - Multiple signaling pathways converge on the Cbfa1/Runx2 transcription factor to regulate osteoblast differentiationRenny T Franceschi
Department of Periodontics Prevention Geriatrics, School of Medicine, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
Connect Tissue Res 44:109-16. 2003..These findings suggest that Runx2 plays a central role in coordinating multiple signals involved in osteoblast differentiation... - Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathwaysRenny T Franceschi
Department of Periodontics, School of Dentistry, Ann Arbor, Michigan 48109 1078, USA
J Cell Biochem 88:446-54. 2003..These findings suggest that Runx2 plays a central role in coordinating multiple signals involved in osteoblast differentiation... - Differentiation-dependent association of phosphorylated extracellular signal-regulated kinase with the chromatin of osteoblast-related genesYan Li
Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
J Bone Miner Res 25:154-63. 2010.... - Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2Guozhi Xiao
Department of Periodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Biol Chem 277:36181-7. 2002..This study is the first demonstration that Runx2 is phosphorylated and activated by FGF-2 via the MAPK pathway and suggests that FGF-2 plays an important role in regulation of Runx2 function and bone formation... - Parathyroid hormone induction of the osteocalcin gene. Requirement for an osteoblast-specific element 1 sequence in the promoter and involvement of multiple-signaling pathwaysDi Jiang
Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Biol Chem 279:5329-37. 2004..Collectively, these studies establish that actions of PTH on the osteocalcin gene are mediated by multiple signaling pathways and require OSE1 and associated nuclear proteins... 
Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal developmentChunxi Ge
Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA
J Cell Biol 176:709-18. 2007..This work establishes an important in vivo function for the ERK-MAPK pathway in bone that involves stimulation of RUNX2 phosphorylation and transcriptional activity...- Impact of the mitogen-activated protein kinase pathway on parathyroid hormone-related protein actions in osteoblastsChen Chen
Department of Periodontics Prevention Geriatrics, University of Michigan, Ann Arbor, Michigan 48109, USA
J Biol Chem 279:29121-9. 2004..These novel findings indicate that the MAPK pathway plays a selective but important role in the actions of PTHrP... - Cooperative interactions between RUNX2 and homeodomain protein-binding sites are critical for the osteoblast-specific expression of the bone sialoprotein geneHernan Roca
Department of Periodontics, Prevention, and Geriatrics and Center for Craniofacial Regeneration, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
J Biol Chem 280:30845-55. 2005..Taken together, our data show that RUNX2 is a direct regulator of Bsp in osteoblasts and that it functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression... - In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiationShuying Yang
Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Bone Miner Res 18:705-15. 2003..These results suggest that Runx2 and BMP2 have distinct, but complementary, roles in osteogenesis and that their combined actions may be necessary for optimal bone formation... - Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cellsGuozhi Xiao
Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109-1078, USA
J Bone Miner Res 17:101-10. 2002..This work establishes that autocrine BMP production as well as integrin-mediated cell-collagen interactions are both required for osteoblast differentiation, and both these pathways require MAP kinase activity... - Bone morphogenetic protein 2 induces dental follicle cells to differentiate toward a cementoblast/osteoblast phenotypeMing Zhao
Department of Periodontics Prevention Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109 1078, USA
J Bone Miner Res 17:1441-51. 2002..These results suggest that BMP-2 triggers follicle cells to differentiate toward a cementoblast/osteoblast phenotype and that the MAPK pathway is involved... - A homeodomain protein binding element in the bone sialoprotein promoter is critical for tissue-specific expression in boneRajaram Gopalakrishnan
Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, USA
Connect Tissue Res 44:154-60. 2003..We therefore hypothesize that the OSHE1 site is involved in both the expression of Bsp in mineralizing tissues and the suppression of transcription in nonmineralizing tissues... - Bone morphogenetic protein-transduced human fibroblasts convert to osteoblasts and form bone in vivoR Bruce Rutherford
School of Dentistry, Center for Biorestoration of Oral Health, University of Michigan, 1011 N. University, Ann Arbor, MI 48109-1078, USA
Tissue Eng 8:441-52. 2002..Only the BMP-transduced fibroblasts formed bone within the diffusion chambers in vivo, revealing that BMP transduction induces osteoblastic conversion of these cells... - Gene transfer of the Runx2 transcription factor enhances osteogenic activity of bone marrow stromal cells in vitro and in vivoZhuoran Zhao
Program in Oral Health Sciences, University of Michigan, 1011 North University Avenue, Ann Arbor, MI 48109, USA
Mol Ther 12:247-53. 2005..Taken together, these studies indicate that Runx2 gene transfer may be an effective route to enhance the osteogenic potential of MSCs... - Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitationHernan Roca
Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
Nucleic Acids Res 36:1723-30. 2008.... - Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell nicheYounghun Jung
Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, 1011 North University Avenue, Ann Arbor, Michigan 48109 1078, USA
Stem Cells 26:2042-51. 2008..Instead, they directly participate in bone formation and niche activities. Disclosure of potential conflicts of interest is found at the end of this article... - Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7Ming Zhao
Department of Periodontics, Prevention, and Geriatrics, School of Dentistry and Center for Craniofacial Regeneration, University of Michigan, Ann Arbor, Michigan 48109-1078, USA
J Cell Biochem 95:1-16. 2005..These studies show that dramatic enhancement of osteogenesis can be achieved using gene therapy to express specific combinations of interacting regenerative molecules... - FGF2 stimulation of the pyrophosphate-generating enzyme, PC-1, in pre-osteoblast cells is mediated by RUNX2Nan E Hatch
Department of Orthodontics and Pediatric Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Bone Miner Res 24:652-62. 2009..Taken together, our results establish that FGF2 is a specific inducer of PC-1 in pre-osteoblast cells and that FGF2 induces PC-1 expression through a mechanism involving Runx2... - BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotypeMattabhorn Phimphilai
Department of Biological Chemistry, University of Michigan School of Medicine, Ann Arbor, Michigan 48109 1078, USA
J Bone Miner Res 21:637-46. 2006..BMP blocking agents were used to show that RUNX2-dependent osteoblast differentiation and transactivation activity both require BMP signaling and, further, that RUNX2 enhances the responsiveness of cells to BMPs... - Identification and functional characterization of ERK/MAPK phosphorylation sites in the Runx2 transcription factorChunxi Ge
Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Biol Chem 284:32533-43. 2009.... 
Osteoblast differentiation stage-specific expression of the pyrophosphate-generating enzyme PC-1Nan E Hatch
Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, Mich 48109 1078, USA
Cells Tissues Organs 189:65-9. 2009..These results also indicate that PC-1 may play multiple and distinct roles in the development of mineralized tissues...- Cooperative interactions between activating transcription factor 4 and Runx2/Cbfa1 stimulate osteoblast-specific osteocalcin gene expressionGuozhi Xiao
Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
J Biol Chem 280:30689-96. 2005.... - Gene delivery by adenovirusesRenny T Franceschi
Department of Periodontics and Oral Medicine, School of Dentistry, and Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI, USA
Methods Mol Biol 455:137-47. 2008..In this chapter, procedures will be described for adenovirus construction and virus delivery to in vivo sites for measurement of osteogenic activity... - BMP gene delivery for alveolar bone engineering at dental implant defectsCourtney A Dunn
Department of Orthodontics and Pediatric Dentistry, University of Michigan, Ann Arbor, MI 48109, USA
Mol Ther 11:294-9. 2005..In vivo gene therapy of BMP-7 offers potential for alveolar bone engineering applications... - Use of a stringent dimerizer-regulated gene expression system for controlled BMP2 deliveryJeong-Tae Koh
Department of Periodontics and Oral Medicine, School of Dentistry, and Center for Craniofacial Regeneration, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
Mol Ther 14:684-91. 2006..This regulated BMP2 expression system will be extremely useful for examining the role of timing and sequence of BMP delivery on bone regeneration... - The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitroYing Zhang
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109 1078, USA
Biomaterials 31:3231-6. 2010..These results demonstrate that adenovirus vectors driving the expression of transcription factors can be delivered directly from biomaterials to direct cell differentiation... - Parathyroid hormone increases activating transcription factor 4 expression and activity in osteoblasts: requirement for osteocalcin gene expressionShibing Yu
Division of Hematology Oncology, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
Endocrinology 149:1960-8. 2008..Collectively, these studies for the first time demonstrate that PTH increases ATF4 expression and activity and that ATF4 is required for PTH induction of Ocn expression in osteoblasts... - Regulation of matrix Gla protein by parathyroid hormone in MC3T3-E1 osteoblast-like cells involves protein kinase A and extracellular signal-regulated kinase pathwaysSupaporn Suttamanatwong
Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, Minnesota 55455, USA
J Cell Biochem 102:496-505. 2007..Taken together, these results indicate that PTH regulates MGP via both PKA- and ERK-dependent pathways... - Bone sialoprotein gene transfer to periodontal ligament cells may not be sufficient to promote mineralization in vitro or in vivoSema S Hakki
Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey
J Periodontol 77:167-73. 2006..In this study, we examined whether gene transfer of BSP into periodontal ligament (PDL) cells would result in an increased ability of PDL cells to promote mineralization in vitro and in vivo... - Role of matrix Gla protein in parathyroid hormone inhibition of osteoblast mineralizationRajaram Gopalakrishnan
Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, Minn 55455, USA
Cells Tissues Organs 181:166-75. 2005..In addition to MGP, the regulation and possible role of osteopontin, another known regulator of osteoblast mineralization, in PTH-mediated regulation of bone and vascular mineralization is discussed...
Research Grants
- BONE SIALOPROTEIN: EXPRESSION & ROLE IN MINERALIZATIONRenny Franceschi; Fiscal Year: 2004..Such knowledge is essential for designing new strategies for bone regeneration to treat trauma and pathological conditions such as osteoporosis and periodontal disease. ..
- GENE THERAPY APPROACH FOR ENGINEERING CRANIOFACIAL BONERenny Franceschi; Fiscal Year: 2004..These studies will greatly advance our understanding of BMP-dependent bone regeneration and develop a viable gene therapy approach for regenerating bone in the craniofacial and appendicular skeleton. ..
- ECM REGULATION OF OSTEOBLAST GENE EXPRESSIONRenny Franceschi; Fiscal Year: 2005..They will elucidate a novel mechanism to explain how ECM signals control the activity of a critical osteoblast transcription factor, and show how matrix signals interact with signals generated by BMPs to regulate bone formation. ..
- COMBINATORIAL GENE THERAPY FOR BONE REGENERATIONRenny Franceschi; Fiscal Year: 2007..Approaches developed will provide proof of principle of the eventual development of new therapies for bone regeneration that mimic natural biological processes by providing temporal control over regenerative events. ..
- MAP KINASE REGULATION OF OSTEOBLAST FUNCTIONRenny Franceschi; Fiscal Year: 2007....
- MAP KINASE REGULATION OF OSTEOBLAST FUNCTIONRENNY THEODORE FRANCESCHI; Fiscal Year: 2010..7. ..
- COMBINATORIAL GENE THERAPY FOR BONE REGENERATIONRENNY THEODORE FRANCESCHI; Fiscal Year: 2010..Approaches developed will provide proof of principle of the eventual development of new therapies for bone regeneration that mimic natural biological processes by providing temporal control over regenerative events. ..
- ROLES OF ASCORBIC ACID IN OSTEOBLAST DIFFERENTIATIONRenny Franceschi; Fiscal Year: 1999..These studies will enable Dr. Franceschi and his colleagues to gain a better understanding of the mechanisms by which ascorbate affects the programming of bone cell differentiation at the molecular level. ..
- BONE SIALOPROTEIN--EXPRESSION AND ROLE IN MINERALIZATIONRenny Franceschi; Fiscal Year: 2000....
- MAP KINASE REGULATION OF OSTEOBLAST FUNCTIONRENNY THEODORE FRANCESCHI; Fiscal Year: 2010..Therapeutic manipulation of the MAPK pathway has the potential to augment bone anabolic therapies such as weight-bearing exercise to prevent or reverse the deleterious effects of osteoporosis. ..