Brian Fox

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination
    Sandy Thao
    Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 5:267-76. 2004
  2. ncbi request reprint Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies
    Robert C Tyler
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Wisconsin 53706, USA
    Proteins 59:633-43. 2005
  3. pmc Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed β-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E
    Taichi E Takasuka
    Great Lakes Bioenergy Research Center, University of Wisconsin Madison, Madison, Wisconsin, United States of America Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e94166. 2014
  4. pmc Small-scale, semi-automated purification of eukaryotic proteins for structure determination
    Ronnie O Frederick
    The University of Wisconsin Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Room 141B, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 8:153-66. 2007
  5. ncbi request reprint Structural genomics: from genes to structures with valuable materials and many questions in between
    Brian G Fox
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Methods 5:129-32. 2008
  6. ncbi request reprint X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1
    Brian G Fox
    University of Wisconsin Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 53706, USA
    Proteins 61:206-8. 2005
  7. pmc Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase
    Lucas J Bailey
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Protein Expr Purif 57:9-16. 2008
  8. pmc The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana
    Eduard Bitto
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:469-72. 2005
  9. ncbi request reprint Characterization of the nitrosyl adduct of substrate-bound mouse cysteine dioxygenase by electron paramagnetic resonance: electronic structure of the active site and mechanistic implications
    Brad S Pierce
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 46:8569-78. 2007
  10. ncbi request reprint Structures of proteins of biomedical interest from the Center for Eukaryotic Structural Genomics
    George N Phillips
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Struct Funct Genomics 8:73-84. 2007

Collaborators

Detail Information

Publications70

  1. ncbi request reprint Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination
    Sandy Thao
    Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 5:267-76. 2004
    ..Sequence verified entry clones were then used to create expression vectors again via the Gateway system...
  2. ncbi request reprint Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies
    Robert C Tyler
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Wisconsin 53706, USA
    Proteins 59:633-43. 2005
    ..coli platform when successful yields more protein, as currently needed for crystallization trials for X-ray structure determination...
  3. pmc Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed β-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E
    Taichi E Takasuka
    Great Lakes Bioenergy Research Center, University of Wisconsin Madison, Madison, Wisconsin, United States of America Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e94166. 2014
    ....
  4. pmc Small-scale, semi-automated purification of eukaryotic proteins for structure determination
    Ronnie O Frederick
    The University of Wisconsin Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Room 141B, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 8:153-66. 2007
    ....
  5. ncbi request reprint Structural genomics: from genes to structures with valuable materials and many questions in between
    Brian G Fox
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Methods 5:129-32. 2008
    ....
  6. ncbi request reprint X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1
    Brian G Fox
    University of Wisconsin Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 53706, USA
    Proteins 61:206-8. 2005
  7. pmc Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase
    Lucas J Bailey
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Protein Expr Purif 57:9-16. 2008
    ..15 microM and 3.0 s(-1), respectively. This expression system and purification allows for the recovery of the soluble oxidoreductase in yields that facilitate further biochemical and structural characterizations...
  8. pmc The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana
    Eduard Bitto
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:469-72. 2005
    ..The metal-binding site was defined and is similar to the iron(II) binding sites found in other members of the superfamily...
  9. ncbi request reprint Characterization of the nitrosyl adduct of substrate-bound mouse cysteine dioxygenase by electron paramagnetic resonance: electronic structure of the active site and mechanistic implications
    Brad S Pierce
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 46:8569-78. 2007
    ..Moreover, the computed EPR parameters (g and A values) are in excellent agreement with experimental results for this CDO species and those obtained from comparable synthetic {FeNO}7 (S = 1/2) iron-nitrosyl complexes...
  10. ncbi request reprint Structures of proteins of biomedical interest from the Center for Eukaryotic Structural Genomics
    George N Phillips
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Struct Funct Genomics 8:73-84. 2007
    ..Many times proteins qualify in more than one of these categories. Here we review some of the structures that have connections to human health and disease...
  11. pmc X-ray structure of ILL2, an auxin-conjugate amidohydrolase from Arabidopsis thaliana
    Eduard Bitto
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Proteins 74:61-71. 2009
    ..Overall, the structure of ILL2 suggests that this enzyme likely uses a catalytic mechanism that follows the paradigm established for the other enzymes of the M20 peptidase family...
  12. ncbi request reprint Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
    Hassan K Sreenath
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 40:256-67. 2005
    ..These protein preparations have been utilized to solve X-ray crystal structures by multiwavelength anomalous diffraction phasing...
  13. ncbi request reprint Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination
    Robert C Tyler
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 40:268-78. 2005
    ..1H-15N heteronuclear single quantum correlation spectroscopy as well as 13C; 15N-edited spectroscopy showed that the purified [U-15N]- and [U-13C, U-15N]-labeled proteins were suitable for structure analysis...
  14. ncbi request reprint High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics
    Won Bae Jeon
    The Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Bobcock Drive, 53706 1549, USA
    J Struct Funct Genomics 6:143-7. 2005
    ..The protocols have been used successfully to provide high quality proteins that are suitable for structural studies by X-ray crystallography and NMR...
  15. pmc The Center for Eukaryotic Structural Genomics
    John L Markley
    Center for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin Madison, Madison, WI 53706, USA
    J Struct Funct Genomics 10:165-79. 2009
    ....
  16. ncbi request reprint Sulfate requirement for heterotrophic growth of "Ferroplasma acidarmanus" strain fer1
    David J Baumler
    Cellular and Molecular Biology Program, Kaspar Laboratory, University of Wisconsin Madison, 1925 Willow Dr, Madison, WI 53706, USA
    Res Microbiol 156:492-8. 2005
    ..Sulfide was absent in the precipitate but zinc and sulfur were detected. These data demonstrate that SO4 is required for heterotrophic growth of strain fer1 and may have a role in the global sulfur cycle...
  17. pmc The length of the bound fatty acid influences the dynamics of the acyl carrier protein and the stability of the thioester bond
    Gregory A Zornetzer
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin53706, USA
    Biochemistry 49:470-7. 2010
    ..Thus, acyl-ACP offers an interesting system for future investigations of complex protein motions on the micro- and millisecond time scales...
  18. pmc Structure and mechanism of mouse cysteine dioxygenase
    Jason G McCoy
    Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Madison, 53706 1544, USA
    Proc Natl Acad Sci U S A 103:3084-9. 2006
    ..Details of the predicted active site are used to present and discuss a plausible mechanism of action for the enzyme...
  19. doi request reprint Autoinduction of protein expression
    Brian G Fox
    Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin, USA
    Curr Protoc Protein Sci . 2009
    ..Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included...
  20. pmc Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria
    Jason G McCoy
    Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proteins 74:368-77. 2009
    ..1 degrees C. Modeling of cofactor binding in the active site based on the structure of methoxy mycolic acid synthase 2 revealed a number of conserved interactions within the active site...
  21. ncbi request reprint A graphical approach to tracking and reporting target status in structural genomics
    Xiaokang Pan
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 8:209-16. 2007
    ..This software package has facilitated inter-group communication, improved the quality and accuracy of information in our LIMS, and increased the efficiency and accuracy of our reports to PepcDB...
  22. ncbi request reprint X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3
    Thomas E Malone
    Molecular and Environmental Toxicology Program, University of Wisconsin Madison, 53706, USA
    Proteins 58:243-5. 2005
  23. pmc Structure of human J-type co-chaperone HscB reveals a tetracysteine metal-binding domain
    Eduard Bitto
    Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    J Biol Chem 283:30184-92. 2008
    ..The normal mode analysis of HscB revealed that this L-shaped protein preferentially undergoes a scissors-like motion that correlates well with the conformational changes of human HscB observed in the crystals...
  24. ncbi request reprint Combined participation of hydroxylase active site residues and effector protein binding in a para to ortho modulation of toluene 4-monooxygenase regiospecificity
    Kevin H Mitchell
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    Biochemistry 41:3176-88. 2002
    ..Evolutionary and mechanistic implications of these findings are considered...
  25. ncbi request reprint Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N
    Qin Zhao
    Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Struct Funct Genomics 5:87-93. 2004
    ....
  26. pmc Identification of the binding region of the [2Fe-2S] ferredoxin in stearoyl-acyl carrier protein desaturase: insight into the catalytic complex and mechanism of action
    Pablo Sobrado
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 45:4848-58. 2006
    ..These results are considered in the light of the presently available models for Fd-mediated electron transfer in Delta9D and other protein-protein complexes...
  27. ncbi request reprint Structural and functional characterization of a novel phosphatase from the Arabidopsis thaliana gene locus At1g05000
    David J Aceti
    Department of Biochemistry, The Center for Eukaryotic Structural Genomics, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA
    Proteins 73:241-53. 2008
    ..Divalent metal cations were not required for activity and had little effect on the reaction...
  28. pmc Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins
    Pil Seok Chae
    Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
    Nat Methods 7:1003-8. 2010
    ..MNG amphiphiles are promising tools for membrane protein science because of the ease with which they may be prepared and the facility with which their structures may be varied...
  29. ncbi request reprint Fluorescence anisotropy studies of enzyme-substrate complex formation in stearoyl-ACP desaturase
    Jeffrey A Haas
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 41:14472-81. 2002
    ..These results indicate that acyl chain length selectivity derives in major part from partition of the enzyme-substrate complex between substrate release and subsequent steps in catalysis...
  30. pmc Spectroscopic and computational characterization of substrate-bound mouse cysteine dioxygenase: nature of the ferrous and ferric cysteine adducts and mechanistic implications
    Jessica D Gardner
    Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Biochemistry 49:6033-41. 2010
    ....
  31. doi request reprint Crystallographic and catalytic studies of the peroxide-shunt reaction in a diiron hydroxylase
    Lucas J Bailey
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    Biochemistry 48:8932-9. 2009
    ..These results provide a new structural benchmark for how activated intermediates may be formed and dispatched during diiron hydroxylase catalysis...
  32. doi request reprint Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenase
    Nathaniel L Elsen
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    Biochemistry 48:3838-46. 2009
    ..This combination of catalytic and structural studies offers important new insight into the role of the role of conserved Thr-201, and its contributions to the catalytic reaction cycle...
  33. pmc X-ray structure of a soluble Rieske-type ferredoxin from Mus musculus
    Elena J Levin
    Department of Biochemistry, University of Wisconsin, Madison, USA
    Acta Crystallogr D Biol Crystallogr 64:933-40. 2008
    ..Based on its high sequence identity, the structure of the human homolog is likely to be modeled well by the mouse Rieske-type ferredoxin structure...
  34. ncbi request reprint Remarkable aliphatic hydroxylation by the diiron enzyme toluene 4-monooxygenase in reactions with radical or cation diagnostic probes norcarane, 1,1-dimethylcyclopropane, and 1,1-diethylcyclopropane
    Luke A Moe
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    Biochemistry 43:15688-701. 2004
    ..The results show a divergence of radical and cation pathways for T4moH-mediated hydroxylation that can be dissected by diagnostic substrate probe rearrangements and by changes in the source of oxygen used for substrate oxygenation...
  35. pmc X-ray structure of Danio rerio secretagogin: A hexa-EF-hand calcium sensor
    Eduard Bitto
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 53706 1544, USA
    Proteins 76:477-83. 2009
    ....
  36. pmc Solution structures of spinach acyl carrier protein with decanoate and stearate
    Gregory A Zornetzer
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706 1549, USA
    Biochemistry 45:5217-27. 2006
    ..These structures demonstrate the conformational flexibility of spinach ACP and suggest how the protein changes to accommodate its myriad binding partners...
  37. pmc Structural consequences of effector protein complex formation in a diiron hydroxylase
    Lucas J Bailey
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 105:19194-8. 2008
    ..This description provides insight into the functional role of effector protein binding in all carboxylate-bridged diiron hydroxylases...
  38. pmc A combined approach to improving large-scale production of tobacco etch virus protease
    Paul G Blommel
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Protein Expr Purif 55:53-68. 2007
    ..coli cell paste). Methods for producing glutathione-S-transferase-tagged TEV with similar yields (approximately 12 mg pure protease fusion per gram of E. coli cell paste) are also reported...
  39. pmc Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition
    Paul G Blommel
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biotechnol Prog 23:585-98. 2007
    ..Upon the basis of the analysis of over 500 independent expression results, combinations of optimized expression media and expression plasmids that gave protein yields of greater than 1000 mug/mL of expression culture were identified...
  40. pmc Insight into the mechanism of aromatic hydroxylation by toluene 4-monooxygenase by use of specifically deuterated toluene and p-xylene
    Kevin H Mitchell
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 100:3784-9. 2003
    ....
  41. ncbi request reprint Rapid-mix and chemical quench studies of ferredoxin-reduced stearoyl-acyl carrier protein desaturase
    Karen S Lyle
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 42:5857-66. 2003
    ..4), indicating C-H bond cleavage does not contribute significantly to the rate-limiting steps of pre-steady-state catalysis. These results were used to assemble and evaluate a minimal kinetic model for Delta9D catalysis...
  42. ncbi request reprint Reactions of the diiron enzyme stearoyl-acyl carrier protein desaturase
    Brian G Fox
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
    Acc Chem Res 37:421-9. 2004
    ..For soluble Delta9D, the natural reaction may initiate at the C-10 position, in contrast to the well-established initial reactivity of the membrane enzyme homologue stearoyl-coenzyme A (CoA) Delta(9) desaturase at the C-9 position...
  43. ncbi request reprint Crystal structure of gene locus At3g16990 from Arabidopsis thaliana
    Paul G Blommel
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Proteins 57:221-2. 2004
  44. ncbi request reprint Desaturation, chain scission, and register-shift of oxygen-substituted fatty acids during reaction with stearoyl-ACP desaturase
    Corina E Rogge
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Biochemistry 41:10141-8. 2002
    ..Possible mechanisms of the acyl chain scission and implications of these results for the desaturation mechanism are considered...
  45. ncbi request reprint Preparation of isotopically labeled spinach acyl-acyl carrier protein for NMR structural studies
    Gregory A Zornetzer
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 46:446-55. 2006
    ....
  46. pmc Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex
    Michael A Goren
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI 53706, USA
    Protein Expr Purif 62:171-8. 2008
    ..The applicability of cell-free translation to the assembly and purification of other integral membrane protein complexes is discussed...
  47. ncbi request reprint Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase
    Luke A Moe
    Department of Biochemistry, University of Wisconsin Madison, USA
    Acta Crystallogr D Biol Crystallogr 62:476-82. 2006
    ..These structural differences are identified as possible contributors to the evolutionary specialization of soluble Rieske-type ferredoxins between the diiron monooxygenases and cis-dihydrodiol-forming dioxygenases...
  48. pmc Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose
    Allison Riederer
    DOE Great Lakes Bioenergy Research Center, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Appl Environ Microbiol 77:1243-53. 2011
    ..The pattern of expression of transcriptional regulators, sigma factors, and signal transducers indicates that response to growth rate is the dominant global mechanism used for control of gene expression in C. thermocellum...
  49. ncbi request reprint Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana
    David W Smith
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Proteins 57:854-7. 2004
  50. ncbi request reprint Component interactions and implications for complex formation in the multicomponent toluene 4-monooxygenase
    Luke A Moe
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    Biochemistry 45:5478-85. 2006
    ..The results indicate that specialization of effector protein function may include specific protein-protein interactions with [2Fe-2S] domains as well as the hydroxylase component...
  51. ncbi request reprint Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins
    Paul G Blommel
    The University of Wisconsin Center for Eukaryotic Structural Genomics, Biophysics Graduate Degree Program and Biochemistry Department, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Anal Biochem 336:75-86. 2005
    ..The assay was also demonstrated as a general tool for monitoring proteolysis of multidomain fusion proteins containing an internal protease site such as are being created in structural genomics studies worldwide...
  52. doi request reprint Cell-free translation of integral membrane proteins into unilamelar liposomes
    Michael A Goren
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
    Methods Enzymol 463:647-73. 2009
    ....
  53. doi request reprint Cell-free protein synthesis technology in NMR high-throughput structure determination
    Shin ichi Makino
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI, USA
    Methods Mol Biol 607:127-47. 2010
    ....
  54. pmc Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa
    Elitza S Sevova
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA
    J Biol Chem 285:20580-7. 2010
    ..These results represent the first application of cell-free synthesis for the rapid preparation and functional annotation of integral membrane proteins and thus illustrate its utility in studying otherwise intractable enzyme systems...
  55. ncbi request reprint Crystal structure of the protein from gene At3g17210 of Arabidopsis thaliana
    Craig A Bingman
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Proteins 57:218-20. 2004
  56. doi request reprint Flexi vector cloning
    Paul G Blommel
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
    Methods Mol Biol 498:55-73. 2009
    ..The protocol also includes transfer of the sequence-verified clones into another Flexi Vector plasmid backbone. Smaller numbers of cloning reactions can be undertaken by appropriate scaling of the indicated reaction volumes...
  57. ncbi request reprint High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
    Paul G Blommel
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin, Madison, 53706 1549, USA
    Protein Expr Purif 47:562-70. 2006
    ..With the proper construction in the flanking sequence of the vector, gene transfer efficiencies of 95-98% were demonstrated...
  58. pmc A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles
    Pablo Sobrado
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Room 141B, 433 Babcock Drive, Madison, WI 53706, USA
    Protein Expr Purif 58:229-41. 2008
    ..The utility of this approach as a delivery method for production and incorporation of monotopic (peripheral) membrane proteins is discussed...
  59. pmc X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv
    David H Dyer
    Department of Biochemistry, 433 Babcock Dr, University of Wisconsin, Madison, Madison, WI 53706, USA
    Protein Sci 14:1508-17. 2005
    ..Instead, the amino group of Lys-76 occupied this position. The relationships between DesA2 and known diiron enzymes are discussed...
  60. pmc One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
    Yong Chang
    Biophysics Graduate Degree Program, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706 1549, USA
    Protein Sci 18:2316-25. 2009
    ..Furthermore, the tunable coexpression strategy used herein should also be applicable in other species with minor modifications...
  61. ncbi request reprint Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein
    George T Lountos
    School of Chemistry and Biochemistry, Parker H Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 44:7131-42. 2005
    ..The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the approximately 33 residue N-terminal region for catalytic activity...
  62. ncbi request reprint Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex
    Lars Skjeldal
    IKB, Agricultural University of Norway, Box 5040, 1432 NLH, Norway
    J Biol Inorg Chem 9:945-53. 2004
    ..Comparisons are made between the present NMR structure, previous paramagnetic NMR studies of T4moC, and the X-ray structures of other members of the Rieske protein family...
  63. ncbi request reprint Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase
    Natasha M Nesbitt
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Protein Expr Purif 39:269-82. 2005
    ..The specific activity of the purified protein is 0.541 U mg(-1), indicating a turnover number of approximately 0.2 s(-1), and the apparent Km values for octanoyl-ACP and apo-H protein are 10.2+/-4.4 and 13.2+/-2.9 microM, respectively...
  64. ncbi request reprint Crystallization and preliminary analysis of native and N-terminal truncated isoforms of toluene-4-monooxygenase catalytic effector protein
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 59:572-5. 2003
    ..These crystallographic results are the first available for the evolutionarily related but functionally diversified catalytic effector proteins from the multicomponent diiron monooxygenase family...
  65. ncbi request reprint Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:957-61. 2004
    ..Thus, the local symmetry produces approximate systematic absences along the (00l) principal axis and complicates the space-group determination...
  66. ncbi request reprint Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:1289-91. 2004
    ..6 A. The asymmetric unit is likely to contain at least two XenB molecules (V(M) = 3.1 A(3) Da(-1), 60% solvent) and a molecular-replacement solution has been determined in order to solve the structure...
  67. ncbi request reprint Reaction mechanisms of non-heme diiron hydroxylases characterized in whole cells
    Erin Bertrand
    Department of Chemistry, Bates College, 5 Andrews Rd Lewiston, ME 04240, United States
    J Inorg Biochem 99:1998-2006. 2005
    ..No radical lifetime dependence on substrate concentration was seen. Results from experiments with diethylcyclopropane, spirooctane, dimethylcyclopropane, and diethylcyclopropane are consistent with a restricted active site for AlkB...
  68. ncbi request reprint N-isotope effects on the Raman spectra of Fe(2)S(2) ferredoxin and Rieske ferredoxin: evidence for structural rigidity of metal sites
    Frederik J Rotsaert
    Department of Biochemistry and Molecular Biology, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006 8921, USA
    J Biol Inorg Chem 8:318-26. 2003
    ..In both cases, the structural rigidity is likely to play a role in minimizing the reorganization energy for electron transfer...
  69. doi request reprint Geometric and electronic structure studies of the binuclear nonheme ferrous active site of toluene-4-monooxygenase: parallels with methane monooxygenase and insight into the role of the effector proteins in O2 activation
    Jennifer K Schwartz
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 130:7098-109. 2008
    ....
  70. pmc Identification of transcribed sequences in Arabidopsis thaliana by using high-resolution genome tiling arrays
    Viktor Stolc
    Genome Research Facility, National Aeronautics and Space Administration Ames Research Center, Moffett Field, CA 94035, USA
    Proc Natl Acad Sci U S A 102:4453-8. 2005
    ..Transcription activities were also detected for many of the intergenic regions and the small RNAs, including tRNA, small nuclear RNA, small nucleolar RNA, and microRNA. Expression of tRNAs correlates with genome-wide amino acid usage...

Research Grants14

  1. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2007
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  2. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2006
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  3. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2005
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  4. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2004
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  5. ROLE OF IRON OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2003
    ..The proposed studies will also reveal the molecular details of reaction with non-natural trans fatty acids, which are increasingly found in human diet from the consumption of partially hydrogenated plant oils. ..
  6. ROLE OF IRON OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2002
    ..The proposed studies will also reveal the molecular details of reaction with non-natural trans fatty acids, which are increasingly found in human diet from the consumption of partially hydrogenated plant oils. ..
  7. ROLE OF IRON OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2001
    ..The proposed studies will also reveal the molecular details of reaction with non-natural trans fatty acids, which are increasingly found in human diet from the consumption of partially hydrogenated plant oils. ..
  8. ROLE OF IRON OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2000
    ..The proposed studies will also reveal the molecular details of reaction with non-natural trans fatty acids, which are increasingly found in human diet from the consumption of partially hydrogenated plant oils. ..
  9. IRON OXO CLUSTERS AND FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 1999
    ..Both of these outcomes will help to further define the principles which govern O2-activation by the proteins containing iron-oxo clusters. ..