TIMOTHY G FORMOSA

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure
    Tim Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 162:1557-71. 2002
  2. ncbi FACT and the reorganized nucleosome
    Tim Formosa
    University of Utah School of Medicine, Department of Biochemistry, 15 N Medical Drive East RM 4100, Salt Lake City, UT 84112 5650, USA
    Mol Biosyst 4:1085-93. 2008
  3. ncbi Avoiding a fatal attraction: properties of nucleosomes and a histone chaperone revealed under physiological conditions
    Tim Formosa
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112, USA
    Mol Cell 37:747-8. 2010
  4. ncbi The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell 22:363-74. 2006
  5. ncbi Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes
    Susan Ruone
    University of Utah School of Medicine, Department of Biochemistry, Salt Lake City, Utah 84132, USA
    J Biol Chem 278:45288-95. 2003
  6. ncbi Opposing roles for Set2 and yFACT in regulating TBP binding at promoters
    Debabrata Biswas
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, 84112, USA
    EMBO J 25:4479-89. 2006
  7. ncbi Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    J Biol Chem 283:5058-68. 2008
  8. ncbi The yeast FACT complex has a role in transcriptional initiation
    Debabrata Biswas
    Department of Pathology, University of Utah Health Sciences Center, 30 North 1900 East, Salt Lake City, Utah 84132 2501, USA
    Mol Cell Biol 25:5812-22. 2005
  9. ncbi Structural features of nucleosomes reorganized by yeast FACT and its HMG box component, Nhp6
    Alison R Rhoades
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Mol Cell Biol 24:3907-17. 2004
  10. ncbi yFACT induces global accessibility of nucleosomal DNA without H2A-H2B displacement
    Hua Xin
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84112, USA
    Mol Cell 35:365-76. 2009

Research Grants

  1. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    Timothy Formosa; Fiscal Year: 2009
  2. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    TIMOTHY G FORMOSA; Fiscal Year: 2010
  3. Biochemical & Genetic Analysis of Yeast SPN
    Timothy Formosa; Fiscal Year: 2005
  4. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    Timothy Formosa; Fiscal Year: 2006

Collaborators

  • David Stillman
  • Heidi L Schubert
  • Debabrata Biswas
  • Hua Xin
  • Andrew P VanDemark
  • Christopher P Hill
  • Laura McCullough
  • Alison R Rhoades
  • Kianoush Sadre-Bazzaz
  • Susan Ruone
  • Shinya Takahata
  • Mary Blanksma
  • Annie Heroux
  • Rinku Dutta-Biswas
  • Yaxin Yu
  • Shannon McCullock
  • Frank G Whitby
  • Howard Robinson
  • Shayla Bentley
  • Robert Rawlins
  • Todd Kinard
  • Elliott Ferris
  • Yoichiro Shibata
  • Brian D Strahl
  • Doyel Mitra
  • Matthew Prall

Detail Information

Publications13

  1. ncbi Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure
    Tim Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 162:1557-71. 2002
    ..Mutations that impair the reassembly activity cause chromatin to accumulate in an abnormally disrupted state, imposing a requirement for a nucleosome reassembly function that we propose is provided by Hir/Hpc proteins...
  2. ncbi FACT and the reorganized nucleosome
    Tim Formosa
    University of Utah School of Medicine, Department of Biochemistry, 15 N Medical Drive East RM 4100, Salt Lake City, UT 84112 5650, USA
    Mol Biosyst 4:1085-93. 2008
    ..quot; The structures of two domains of FACT have been determined and they reveal multiple potential interaction sites. Roles for these binding sites in FACT function and regulation are discussed...
  3. ncbi Avoiding a fatal attraction: properties of nucleosomes and a histone chaperone revealed under physiological conditions
    Tim Formosa
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112, USA
    Mol Cell 37:747-8. 2010
    ....
  4. ncbi The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell 22:363-74. 2006
    ..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process...
  5. ncbi Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes
    Susan Ruone
    University of Utah School of Medicine, Department of Biochemistry, Salt Lake City, Utah 84132, USA
    J Biol Chem 278:45288-95. 2003
    ..These results suggest that Nhp6 and the related high mobility group B proteins may have a general role in promoting rearrangements of chromatin by initiating the destabilization of core nucleosomal structure...
  6. ncbi Opposing roles for Set2 and yFACT in regulating TBP binding at promoters
    Debabrata Biswas
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, 84112, USA
    EMBO J 25:4479-89. 2006
    ..These synthetic defects are suppressed by set2, demonstrating that yFACT and Set2 oppose one another during transcriptional initiation at a step involving DNA binding by TBP and TFIIA...
  7. ncbi Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    J Biol Chem 283:5058-68. 2008
    ..The docking domain of H2A is identified as an important participant in maintaining stability during yFACT-mediated nucleosome reorganization, suggesting new models for the mechanism of this activity...
  8. ncbi The yeast FACT complex has a role in transcriptional initiation
    Debabrata Biswas
    Department of Pathology, University of Utah Health Sciences Center, 30 North 1900 East, Salt Lake City, Utah 84132 2501, USA
    Mol Cell Biol 25:5812-22. 2005
    ..Thus, yFACT functions in establishing transcription initiation complexes in addition to the previously described role in elongation...
  9. ncbi Structural features of nucleosomes reorganized by yeast FACT and its HMG box component, Nhp6
    Alison R Rhoades
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Mol Cell Biol 24:3907-17. 2004
    ....
  10. ncbi yFACT induces global accessibility of nucleosomal DNA without H2A-H2B displacement
    Hua Xin
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84112, USA
    Mol Cell 35:365-76. 2009
    ..We propose that yFACT promotes a reversible transition between two nucleosomal forms, and that this activity contributes to the establishment and maintenance of the chromatin barrier as well as to overcoming it...
  11. ncbi A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae
    Debabrata Biswas
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA
    Genetics 178:649-59. 2008
    ..The pob3 defect in S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication...
  12. ncbi blm3-1 is an allele of UBP3, a ubiquitin protease that appears to act during transcription of damaged DNA
    Shannon McCullock
    University of Utah School of Medicine, Department of Biochemistry, 15 N Medical Drive East RM 4100, Salt Lake City, UT 84112 5640, USA
    J Mol Biol 363:660-72. 2006
    ....
  13. ncbi Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening
    Kianoush Sadre-Bazzaz
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Mol Cell 37:728-35. 2010
    ..We also demonstrate that Blm10 acts to maintain mitochondrial function. Consistent with the structural data, the C-terminal residues of Blm10 are needed for this activity...

Research Grants9

  1. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    Timothy Formosa; Fiscal Year: 2009
    ..yFACT is a vital and novel component of both processes, so studying it provides insight into several distinct central pathways simultaneously. ..
  2. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    TIMOTHY G FORMOSA; Fiscal Year: 2010
    ..yFACT is a vital and novel component of both processes, so studying it provides insight into several distinct central pathways simultaneously. ..
  3. Biochemical & Genetic Analysis of Yeast SPN
    Timothy Formosa; Fiscal Year: 2005
    ....
  4. Biochemical and Genetic Analysis of yFACT, A Novel Nucleosome Reorganizing Factor
    Timothy Formosa; Fiscal Year: 2006
    ..yFACT is a vital and novel component of both processes, so studying it provides insight into several distinct central pathways simultaneously. ..