T Formosa

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi request reprint Suppressors of the temperature sensitivity of DNA polymerase alpha mutations in Saccharomyces cerevisiae
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Mol Gen Genet 257:461-8. 1998
  2. pmc Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, 50 N Medical Drive Rm 211, Salt Lake City, UT 84132, USA
    EMBO J 20:3506-17. 2001
  3. ncbi request reprint Changing the DNA landscape: putting a SPN on chromatin
    T Formosa
    University of Utah, Biochemistry, 20 N 1900 E RM 211, Salt Lake City, UT 84132 3201, USA
    Curr Top Microbiol Immunol 274:171-201. 2003
  4. pmc The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein
    J Wittmeyer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Mol Cell Biol 17:4178-90. 1997
  5. pmc POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae
    M B Schlesinger
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 155:1593-606. 2000
  6. pmc Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 151:1459-70. 1999
  7. ncbi request reprint Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha
    J Wittmeyer
    Department of Biochemistry, University of Utah, Salt Lake City 84132, USA
    Biochemistry 38:8961-71. 1999
  8. pmc Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo
    J Miles
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132
    Mol Cell Biol 12:5724-35. 1992
  9. pmc Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism
    J D Singer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell Biol 16:1356-66. 1996

Collaborators

  • P Eriksson
  • Y Yu
  • J Wittmeyer
  • M B Schlesinger
  • J D Singer
  • L Joss
  • B M Manning
  • J Miles

Detail Information

Publications9

  1. ncbi request reprint Suppressors of the temperature sensitivity of DNA polymerase alpha mutations in Saccharomyces cerevisiae
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Mol Gen Genet 257:461-8. 1998
    ..We also describe a transposon-mediated strategy that should be generally effective for rapid characterization of multicopy suppressors...
  2. pmc Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, 50 N Medical Drive Rm 211, Salt Lake City, UT 84132, USA
    EMBO J 20:3506-17. 2001
    ..These complexes have altered electrophoretic mobility and a distinct pattern of enhanced sensitivity to DNase I. These results suggest that Spt16-Pob3 and Nhp6 cooperate to function as a novel nucleosome reorganizing factor...
  3. ncbi request reprint Changing the DNA landscape: putting a SPN on chromatin
    T Formosa
    University of Utah, Biochemistry, 20 N 1900 E RM 211, Salt Lake City, UT 84132 3201, USA
    Curr Top Microbiol Immunol 274:171-201. 2003
    ....
  4. pmc The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein
    J Wittmeyer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Mol Cell Biol 17:4178-90. 1997
    ..Based on these results and because Cdc68 has been implicated as a regulator of chromatin structure, we postulate that polymerase alpha may interact with these proteins to gain access to its template or to origins of replication in vivo...
  5. pmc POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae
    M B Schlesinger
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 155:1593-606. 2000
    ..Direct examination of DNA contents by flow cytometry showed that S phase onset and progression were delayed when POB3 was mutated. We conclude that Pob3 is required for normal replication as well as for transcription...
  6. pmc Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth
    T Formosa
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Genetics 151:1459-70. 1999
    ..Dna2 therefore appears to act in repair or lagging strand synthesis together with Pol alpha and Ctf4, in a role that is optimal with, but does not require, full helicase activity...
  7. ncbi request reprint Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha
    J Wittmeyer
    Department of Biochemistry, University of Utah, Salt Lake City 84132, USA
    Biochemistry 38:8961-71. 1999
    ..Some of the Spt16-Pob3 complex was found to copurify with the yeast DNA polymerase alpha/primase complex, further supporting a connection between Spt16-Pob3 and DNA replication...
  8. pmc Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo
    J Miles
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132
    Mol Cell Biol 12:5724-35. 1992
    ..These phenotypes are consistent with defective DNA metabolism in cells lacking POB1 and strongly suggest that this DNA polymerase alpha-binding protein plays a role in accurately duplicating the genome in vivo...
  9. pmc Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism
    J D Singer
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell Biol 16:1356-66. 1996
    ..We conclude that a ubiquitin-mediated signaling event directly involving the ubiquitin hydrolase encoded by DOA4 is needed in S. cerevisiae to prevent uncoordinated DNA replication...