Genomes and Genes
Alfred L Fisher
Affiliation: University of Pittsburgh
- The nuclear hormone receptor DAF-12 has opposing effects on Caenorhabditis elegans lifespan and regulates genes repressed in multiple long-lived wormsAlfred L Fisher
Division of Geriatrics, Department of Medicine, University of California, San Francisco, 94121, USA
Aging Cell 5:127-38. 2006..Our results point to daf-12 modulating aging and stress responses in part through the repression of specific genes, and emphasize the role that the repression of genes that curtail maximal lifespan plays in lifespan determination...
- The Caenorhabditis elegans K10C2.4 gene encodes a member of the fumarylacetoacetate hydrolase family: a Caenorhabditis elegans model of type I tyrosinemiaAlfred L Fisher
Department of Medicine, Division of Geriatric Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA
J Biol Chem 283:9127-35. 2008..We also use our model to identify genes that suppress the damage produced by K10C2.4 RNAi in a pilot genetic screen. Our results establish worms as a model for the study of type I tyrosinemia...
- skn-1-Dependent and -independent regulation of aip-1 expression following metabolic stress in Caenorhabditis elegansAnnabel A Ferguson
University of Pittsburgh, Department of Medicine, Division of Geriatric Medicine, 3471 5th Ave, Suite 500, Pittsburgh, PA 15260, USA
Mol Cell Biol 30:2651-67. 2010..These may be triggered by proteosome dysfunction, as we find that this event links the multiple inducers of aip-1. Together, our results show that cell stress triggers aip-1 expression by both skn-1-dependent and -independent pathways...
- Improved vectors for selection of transgenic Caenorhabditis elegansAnnabel A Ferguson
Division of Geriatric Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
Methods Mol Biol 940:87-102. 2013..Here we describe methods to circumvent these limitations and use either homologous recombination or Cre-LoxP mediated recombination in Escherichia coli to insert the unc-119 marker on to a variety of vector backbones...
- Identification of novel genes involved in sarcopenia through RNAi screening in Caenorhabditis elegansLuv Kashyap
Division of Geriatric Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USA
J Gerontol A Biol Sci Med Sci 67:56-65. 2012..The nematode Caenorhabditis elegans might be a novel animal model for sarcopenia as worms experience sarcopenia during aging and mutations affecting the daf-2/insulin-like signaling pathway are able to delay this process...
- Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genesLiquan Cai
Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA
J Biol Chem 286:35915-21. 2011..Together, these results reveal important roles for the eaf-1 and ell-1 genes in the regulation of extracellular matrix components...
- The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activationAnna A Powolny
Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA
Exp Gerontol 46:441-52. 2011..Together our data suggest that DATS is able to increase worm lifespan by enhancing the function of the pro-longevity transcription factor skn-1...
- A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineeringYue Zhang
University of Pittsburgh, Department of Medicine, Division of Geriatric Medicine and Pittsburgh Institute for Neurodegenerative Diseases, Pittsburgh, PA 15260, USA
BMC Dev Biol 8:119. 2008..Additionally, some groups have encountered problems with employing these techniques. Hence, we sought identify ways to improve the simplicity and reliability of the procedure...
- DAF-12 regulates a connected network of genes to ensure robust developmental decisionsDaniel Hochbaum
Division of Geriatric Medicine, Department of Medicine, University of Pittsburgh, Pennsylvania, United States of America
PLoS Genet 7:e1002179. 2011..Together these data are consistent with daf-12 acting to ensure developmental robustness by committing the animal to adult or dauer developmental programs despite variable internal or external conditions...
- TATN-1 mutations reveal a novel role for tyrosine as a metabolic signal that influences developmental decisions and longevity in Caenorhabditis elegansAnnabel A Ferguson
Division of Geriatric Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
PLoS Genet 9:e1004020. 2013..Our findings point to a novel role for tyrosine as a developmental regulator and modulator of longevity, and support a model where elevated tyrosine levels play a causal role in the development of diabetes and cancer in people. ..
- Of worms and women: sarcopenia and its role in disability and mortalityAlfred L Fisher
Department of Medicine, Division of Geriatrics, University of California at San Francisco, San Francisco, California, USA
J Am Geriatr Soc 52:1185-90. 2004..These findings also suggest that C. elegans will develop into an important model system in which to study the biochemical and genetics events responsible for sarcopenia and to test therapeutics designed to prevent or reverse sarcopenia...
- DAF-12-dependent rescue of dauer formation in Caenorhabditis elegans by (25S)-cholestenoic acidJason M Held
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA
Aging Cell 5:283-91. 2006..These data suggest that carboxylated sterols may be key determinants of life history...
- Lipophilic regulator of a developmental switch in Caenorhabditis elegansMatthew S Gill
Buck Institute, 8001 Redwood Boulevard, Novato, CA 94945, USA
Aging Cell 3:413-21. 2004..We propose that the dauer rescuing activity is a hormone synthesized by DAF-9 that acts through DAF-12...
- daf-12 Target Genes during C. elegans agingAlfred Fisher; Fiscal Year: 2005..abstract_text> ..