ANTHONY FINK

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Protein aggregation: folding aggregates, inclusion bodies and amyloid
    A L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Fold Des 3:R9-23. 1998
  2. ncbi Discrete intermediates versus molten globule models for protein folding: characterization of partially folded intermediates of apomyoglobin
    A L Fink
    Department of Chemistry and Biochemistry, The University of California, Santa Cruz 95064, USA
    Fold Des 3:19-25. 1998
  3. ncbi The aggregation and fibrillation of alpha-synuclein
    Anthony L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Acc Chem Res 39:628-34. 2006
  4. ncbi Biophysical properties of the synucleins and their propensities to fibrillate: inhibition of alpha-synuclein assembly by beta- and gamma-synucleins
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 277:11970-8. 2002
  5. ncbi Natively unfolded proteins
    Anthony L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Curr Opin Struct Biol 15:35-41. 2005
  6. ncbi Stimulation of insulin fibrillation by urea-induced intermediates
    Atta Ahmad
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 279:14999-5013. 2004
  7. ncbi Partially folded intermediates in insulin fibrillation
    Atta Ahmad
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Biochemistry 42:11404-16. 2003
  8. doi Impact of Tyr to Ala mutations on alpha-synuclein fibrillation and structural properties
    Natasa Poklar Ulrih
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95964, USA
    Biochim Biophys Acta 1782:581-5. 2008
  9. ncbi Conformational behavior of human alpha-synuclein is modulated by familial Parkinson's disease point mutations A30P and A53T
    Jie Li
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Neurotoxicology 23:553-67. 2002
  10. ncbi Role of individual methionines in the fibrillation of methionine-oxidized alpha-synuclein
    Mark J Hokenson
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 43:4621-33. 2004

Collaborators

Detail Information

Publications71

  1. ncbi Protein aggregation: folding aggregates, inclusion bodies and amyloid
    A L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Fold Des 3:R9-23. 1998
    ..Substantial data support the hypothesis that partially folded intermediates are key precursors to aggregates, that aggregation involves specific intermolecular interactions and that most aggregates involve beta sheets...
  2. ncbi Discrete intermediates versus molten globule models for protein folding: characterization of partially folded intermediates of apomyoglobin
    A L Fink
    Department of Chemistry and Biochemistry, The University of California, Santa Cruz 95064, USA
    Fold Des 3:19-25. 1998
    ..The 'molten globule' has been proposed to be a general intermediate in protein folding. Relatively little is known about the structure of partially folded intermediates, however...
  3. ncbi The aggregation and fibrillation of alpha-synuclein
    Anthony L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Acc Chem Res 39:628-34. 2006
    ..It is apparent that neuronal cells must have a very fine balance of factors that control the levels and potential aggregation of alpha-synuclein...
  4. ncbi Biophysical properties of the synucleins and their propensities to fibrillate: inhibition of alpha-synuclein assembly by beta- and gamma-synucleins
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 277:11970-8. 2002
    ..The lack of fibrils formed by beta-synuclein is most readily explained by the absence of a stretch of hydrophobic residues from the middle region of the protein. A model for the inhibition is proposed...
  5. ncbi Natively unfolded proteins
    Anthony L Fink
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Curr Opin Struct Biol 15:35-41. 2005
    ..New algorithms have been developed to identify disordered regions of proteins and have demonstrated their presence in cancer-associated proteins and proteins regulated by phosphorylation...
  6. ncbi Stimulation of insulin fibrillation by urea-induced intermediates
    Atta Ahmad
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 279:14999-5013. 2004
    ..The correlation between fibrillation and the partially unfolded monomer indicates that the latter is a critical amyloidogenic intermediate in insulin fibrillation...
  7. ncbi Partially folded intermediates in insulin fibrillation
    Atta Ahmad
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Biochemistry 42:11404-16. 2003
    ....
  8. doi Impact of Tyr to Ala mutations on alpha-synuclein fibrillation and structural properties
    Natasa Poklar Ulrih
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95964, USA
    Biochim Biophys Acta 1782:581-5. 2008
    ..These observations suggest that the long-range intramolecular interactions between the N- and C-termini of alpha-synuclein are likely to be crucial to the fibrillation process...
  9. ncbi Conformational behavior of human alpha-synuclein is modulated by familial Parkinson's disease point mutations A30P and A53T
    Jie Li
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Neurotoxicology 23:553-67. 2002
    ....
  10. ncbi Role of individual methionines in the fibrillation of methionine-oxidized alpha-synuclein
    Mark J Hokenson
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 43:4621-33. 2004
    ..The presence of zinc was shown to overcome the Met oxidation-induced inhibition. Interestingly, substitution of Met by Leu led to increased propensity for aggregation (soluble oligomers) but slower formation of fibrils...
  11. ncbi Effect of association state and conformational stability on the kinetics of immunoglobulin light chain amyloid fibril formation at physiological pH
    Pierre O Souillac
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Biol Chem 277:12657-65. 2002
    ..Thus, increasing concentrations of urea, by triggering dissociation of dimeric LEN, lead to increased rates of fibrillation...
  12. ncbi Early events in the fibrillation of monomeric insulin
    Atta Ahmad
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 280:42669-75. 2005
    ..Both have significantly non-native conformations, and indicate that fibrillation occurs from a beta-rich structure significantly distinct from the native fold...
  13. ncbi Conformational behavior and aggregation of alpha-synuclein in organic solvents: modeling the effects of membranes
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 42:2720-30. 2003
    ..At least to some extent, these conformational effects mimic those observed in the presence of phospholipid vesicles, and can explain some of the observed effects of membranes on alpha-synuclein fibrillation...
  14. ncbi The association of alpha-synuclein with membranes affects bilayer structure, stability, and fibril formation
    Min Zhu
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 278:40186-97. 2003
    ..Protofibrillar or fibrillar alpha-synuclein caused a much more rapid destruction of the membrane than soluble monomeric alpha-synuclein, indicating that protofibrils (oligomers) or fibrils are likely to be significantly neurotoxic...
  15. ncbi Certain metals trigger fibrillation of methionine-oxidized alpha-synuclein
    Ghiam Yamin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 278:27630-5. 2003
    ..These findings indicate that a combination of oxidative stress and environmental metal pollution could play an important role in triggering the fibrillation of alpha-synuclein and thus possibly Parkinson's disease...
  16. pmc A general model for amyloid fibril assembly based on morphological studies using atomic force microscopy
    Ritu Khurana
    Department of Chemistry, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Biophys J 85:1135-44. 2003
    ..In all cases, protofilaments intertwine to form protofibrils, and protofibrils intertwine to form fibrils. We propose that the hierarchical assembly model describes a general mechanism of assembly for all amyloid fibrils...
  17. ncbi The flavonoid baicalein inhibits fibrillation of alpha-synuclein and disaggregates existing fibrils
    Min Zhu
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 279:26846-57. 2004
    ..These observations suggest that baicalein and similar compounds may have potential as therapeutic leads in combating Parkinson's disease and that diets rich in flavonoids may be effective in preventing the disorder...
  18. ncbi Conformational prerequisites for alpha-lactalbumin fibrillation
    John Goers
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 41:12546-51. 2002
    ....
  19. ncbi A model for amyloid fibril formation in immunoglobulin light chains based on comparison of amyloidogenic and benign proteins and specific antibody binding
    Ritu Khurana
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Amyloid 10:97-109. 2003
    ..The antibody result indicates that each protofilament is asymmetric with different ends. A model for the formation of fibrils by SMA is proposed...
  20. ncbi Heparin and other glycosaminoglycans stimulate the formation of amyloid fibrils from alpha-synuclein in vitro
    Jeffrey A Cohlberg
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 41:1502-11. 2002
    ..Since there is some evidence that Lewy bodies may contain GAGs, these observations may be very relevant in the context of the etiology of Parkinson's disease...
  21. ncbi Elucidation of the molecular mechanism during the early events in immunoglobulin light chain amyloid fibrillation. Evidence for an off-pathway oligomer at acidic pH
    Pierre O Souillac
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Biol Chem 277:12666-79. 2002
    ....
  22. ncbi Nuclear localization of alpha-synuclein and its interaction with histones
    John Goers
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 42:8465-71. 2003
    ..e., injections of the herbicide paraquat). These observations indicate that translocation into the nucleus and binding with histones represent potential mechanisms underlying alpha-synuclein pathophysiology...
  23. ncbi Structural transformations of oligomeric intermediates in the fibrillation of the immunoglobulin light chain LEN
    Pierre O Souillac
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 42:8094-104. 2003
    ....
  24. ncbi Forcing nonamyloidogenic beta-synuclein to fibrillate
    Ghiam Yamin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 44:9096-107. 2005
    ..These new findings demonstrate the potential effect of environmental pollutants in generating an amyloidogenic, and potentially neurotoxic, conformation, in an otherwise benign protein...
  25. ncbi Conformational prerequisites for formation of amyloid fibrils from histones
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Mol Biol 342:1305-24. 2004
    ..HCl increased the rate of fibrillation, although much less than NaCl. Different ions also differentially affected the rate of nucleation and the rate of fibril elongation...
  26. pmc Structural characteristics of alpha-synuclein oligomers stabilized by the flavonoid baicalein
    Dong Pyo Hong
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 383:214-23. 2008
    ..This effect was rather similar to that of the monomeric protein, suggesting that targeted stabilization of certain alpha-synuclein oligomers might offer a potential strategy for the development of novel Parkinson's disease therapies...
  27. pmc Guiding protein aggregation with macromolecular crowding
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Biochemistry 47:8993-9006. 2008
    ....
  28. ncbi Role of protein-water interactions and electrostatics in alpha-synuclein fibril formation
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 43:3289-300. 2004
    ..Moderate concentrations of anions affected both the rates of nucleation and the elongation of alpha-synuclein fibrillation, primarily via their effect on the interaction of the protein with water...
  29. ncbi Independent heterologous fibrillation of insulin and its B-chain peptide
    Dong Pyo Hong
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 44:16701-9. 2005
    ..A model for the fibrillation of the heterogeneous system of insulin and B-chain insulin is proposed...
  30. ncbi Annular oligomeric amyloid intermediates observed by in situ atomic force microscopy
    Min Zhu
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    J Biol Chem 279:24452-9. 2004
    ..The data indicate that protein aggregation can be very complex, involving a variety of different oligomeric intermediates whose population will be determined by the kinetic and thermodynamic competition between them...
  31. ncbi Lipid binding inhibits alpha-synuclein fibril formation
    Min Zhu
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 278:16873-7. 2003
    ..Given that a significant fraction of alpha-synuclein is membrane-bound in dopaminergic neurons, this observation has significant physiological significance...
  32. pmc The effect of membranes on the in vitro fibrillation of an amyloidogenic light-chain variable-domain SMA
    Xiaoyun Meng
    Department of Chemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 381:989-99. 2008
    ..Thus, membranes may have significant effects on light-chain fibrillation and may contribute to the site selectivity observed in AL amyloidosis...
  33. ncbi Effects of nitration on the structure and aggregation of alpha-synuclein
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Brain Res Mol Brain Res 134:84-102. 2005
    ..The addition of nitrated alpha-synuclein inhibited fibrillation of non-modified alpha-synuclein at neutral pH. Potential implications of these findings to the etiology of Parkinson's disease are discussed...
  34. doi Molecular mechanisms underlying the flavonoid-induced inhibition of alpha-synuclein fibrillation
    Xiaoyun Meng
    Department of Chemistry, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Biochemistry 48:8206-24. 2009
    ..The flavonoids inhibiting alpha-synuclein fibrillation and stabilizing the protein monomeric conformation can serve as a model for the development of therapeutic drugs in combating Parkinson's disease...
  35. ncbi Characterization of oligomeric intermediates in alpha-synuclein fibrillation: FRET studies of Y125W/Y133F/Y136F alpha-synuclein
    Joanna Kaylor
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Mol Biol 353:357-72. 2005
    ..No FRET was observed in the fibrils, due to quenching of Tyr39 fluorescence in the fibril core. Thus, aggregation of alpha-synuclein involves multiple oligomeric intermediates and competing pathways...
  36. ncbi Structural characterization of the partially folded intermediates of an immunoglobulin light chain leading to amyloid fibrillation and amorphous aggregation
    Zhijie Qin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 46:3521-31. 2007
    ..In the case of light chain aggregation, it appears that more native-like intermediate conformations are more prone to form amorphous deposits...
  37. ncbi Effect of 4-hydroxy-2-nonenal modification on alpha-synuclein aggregation
    Zhijie Qin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 282:5862-70. 2007
    ..Our observations indicate that HNE modification of alpha-synuclein prevents fibrillation but may result in toxic oligomers, which could therefore contribute to the demise of neurons subjected to oxidative damage...
  38. ncbi Fibrillation of human insulin A and B chains
    Dong Pyo Hong
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 45:9342-53. 2006
    ..Thus, both A and B chain peptides can form amyloid fibrils, and both are likely to be involved in the interactions leading to the fibrillation of intact insulin...
  39. ncbi Characterization of oligomers during alpha-synuclein aggregation using intrinsic tryptophan fluorescence
    Alexandra Dusa
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 45:2752-60. 2006
    ..These observations demonstrate the utility of Trp fluorescence labeled alpha-synuclein and demonstrate the existence of an oligomeric intermediate that exists as a transient reservoir of alpha-synuclein for fibrillation...
  40. ncbi The oxidation state of DJ-1 regulates its chaperone activity toward alpha-synuclein
    Wenbo Zhou
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Mol Biol 356:1036-48. 2006
    ....
  41. ncbi Conformational constraints for amyloid fibrillation: the importance of being unfolded
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1698:131-53. 2004
    ..The inherent flexibility of such an intermediate is essential in allowing the conformational rearrangements necessary to form the core cross-beta structure of the amyloid fibril...
  42. ncbi The chicken-egg scenario of protein folding revisited
    Vladimir N Uversky
    Institute for Biological Instrumentation, Russian Academy of Sciences, 142292, Moscow Region, Pushchino, Russia
    FEBS Lett 515:79-83. 2002
    ..This correlation provides experimental support for the hypothesis that hydrophobic collapse occurs simultaneously with formation of secondary structure in the early stages of the protein folding...
  43. ncbi Methionine oxidation inhibits fibrillation of human alpha-synuclein in vitro
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    FEBS Lett 517:239-44. 2002
    ..This inhibitory effect was eliminated at low pH. The addition of oxidized alpha-synuclein to the unoxidized form led to a substantial inhibition of alpha-synuclein fibrillation...
  44. ncbi Amino acid determinants of alpha-synuclein aggregation: putting together pieces of the puzzle
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    FEBS Lett 522:9-13. 2002
    ....
  45. ncbi Synergistic effects of pesticides and metals on the fibrillation of alpha-synuclein: implications for Parkinson's disease
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    Neurotoxicology 23:527-36. 2002
    ..We propose a model in which environmentalfactors in conjunction with genetic susceptibility may form the underlying molecular basis for idiopathic PD...
  46. ncbi Accelerated alpha-synuclein fibrillation in crowded milieu
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA
    FEBS Lett 515:99-103. 2002
    ..Our results suggest that the major factor responsible for the accelerated fibrillation under crowded conditions is the excluded volume...
  47. ncbi Effect of zinc and temperature on the conformation of the gamma subunit of retinal phosphodiesterase: a natively unfolded protein
    Vladimir N Uversky
    Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Russia
    J Proteome Res 1:149-59. 2002
    ..Zinc-loaded protein remains effectively unfolded. Possible implications of these findings to the functioning of PDEgamma are discussed...
  48. ncbi Prediction of the association state of insulin using spectral parameters
    Vladimir N Uversky
    Institute for Biological Instrumentation, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia
    J Pharm Sci 92:847-58. 2003
    ..The results show that the spectral properties of the insulins reflect their state of association, and can be used to predict their oligomeric state...
  49. pmc Methionine oxidation stabilizes non-toxic oligomers of alpha-synuclein through strengthening the auto-inhibitory intra-molecular long-range interactions
    Wenbo Zhou
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1802:322-30. 2010
    ..Finally, oxidation of methionines by H2O2 greatly inhibited alpha-Syn fibrillation in vitro, leading to the formation of relatively stable oligomers, which are not toxic to dopaminergic and GABAergic neurons...
  50. ncbi Dopamine and L-dopa disaggregate amyloid fibrils: implications for Parkinson's and Alzheimer's disease
    Jie Li
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    FASEB J 18:962-4. 2004
    ..g., the relationship between fibril formation and neurodegeneration) of protein aggregation. It is also likely to help in the development of strategies for the prevention and treatment of protein deposition diseases...
  51. ncbi Nitration inhibits fibrillation of human alpha-synuclein in vitro by formation of soluble oligomers
    Ghiam Yamin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    FEBS Lett 542:147-52. 2003
    ..These observations suggest that nitration of soluble alpha-synuclein may be a protective factor in PD, rather than a causative one...
  52. ncbi The effect of macromolecular crowding on protein aggregation and amyloid fibril formation
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Mol Recognit 17:456-64. 2004
    ..Pesticides and metals, which are linked to increased risk of Parkinson's disease by epidemiological studies, are shown to accelerate alpha-synuclein fibrillation under conditions of molecular crowding...
  53. ncbi Role of different regions of alpha-synuclein in the assembly of fibrils
    Zhijie Qin
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 46:13322-30. 2007
    ..In contrast, the two terminal regions show no evidence of participating in the assembly of the protofilament core but play a key role in the interactions between the protofilaments, which is necessary for the fibril maturation...
  54. pmc Smoking and Parkinson's disease: does nicotine affect alpha-synuclein fibrillation?
    Dong Pyo Hong
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Biochim Biophys Acta 1794:282-90. 2009
    ..This information can be used to understand the molecular mechanism of the nicotine and hydroquinone action to develop therapeutic solutions for PD...
  55. pmc At low concentrations, 3,4-dihydroxyphenylacetic acid (DOPAC) binds non-covalently to alpha-synuclein and prevents its fibrillation
    Wenbo Zhou
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 388:597-610. 2009
    ....
  56. ncbi Alpha-synuclein can function as an antioxidant preventing oxidation of unsaturated lipid in vesicles
    Min Zhu
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 45:8135-42. 2006
    ..These findings suggest that the inhibition of lipid oxidation by alpha-synuclein may be a physiological function of the protein...
  57. ncbi Surface-catalyzed amyloid fibril formation
    Min Zhu
    Department of Chemistry and Biochemistry and the Department of Physics, University of California, Santa Cruz, California 95064, USA
    J Biol Chem 277:50914-22. 2002
    ....
  58. pmc Polycation-induced oligomerization and accelerated fibrillation of human alpha-synuclein in vitro
    John Goers
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Protein Sci 12:702-7. 2003
    ..The results illustrate the potential critical role of electrostatic interactions in protein aggregation, and the potential role of naturally occurring polycations in modulating alpha-synuclein aggregation...
  59. pmc Effect of methionine oxidation on the structural properties, conformational stability, and aggregation of immunoglobulin light chain LEN
    Dongmei Hu
    Department of Chemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 47:8665-77. 2008
    ..The methionine-oxidized LEN preferred to form amorphous aggregates instead of fibrils. The results indicated that the LEN oxidation may play an important role in amorphous deposition of the protein, but not in its fibrillation...
  60. ncbi Rifampicin inhibits alpha-synuclein fibrillation and disaggregates fibrils
    Jie Li
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Chem Biol 11:1513-21. 2004
    ....
  61. ncbi Fluorescence as a method to reveal structures and membrane-interactions of amyloidogenic proteins
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1768:1862-85. 2007
    ....
  62. ncbi GM1 specifically interacts with alpha-synuclein and inhibits fibrillation
    Zak Martinez
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 46:1868-77. 2007
    ....
  63. pmc Concerted action of metals and macromolecular crowding on the fibrillation of alpha-synuclein
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Protein Pept Lett 15:1079-85. 2008
    ..The faster fibrillation in crowded environments in the presence of heavy metals suggests a simple molecular basis for the observed elevated risk of PD due to exposure to metals...
  64. pmc Tau filaments from human brain and from in vitro assembly of recombinant protein show cross-beta structure
    John Berriman
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 100:9034-8. 2003
    ..Using both these approaches, we show here that native filaments from brain and filaments assembled in vitro from expressed tau protein have a clear cross-beta structure...
  65. ncbi Methionine oxidation, alpha-synuclein and Parkinson's disease
    Charles B Glaser
    Biochim Biophys Acta 1703:157-69. 2005
    ..In addition, if the level of Met-oxidized alpha-synuclein was under the control of methionine sulfoxide reductase (Msr), then this could also be factor in the disease...
  66. ncbi Unraveling multistate unfolding of rabbit muscle creatine kinase
    Irina M Kuznetsova
    Institute of Cytology, Russian Academy of Sciences, 194064 St Petersburg, Russia
    Biochim Biophys Acta 1596:138-55. 2002
    ..A model of CK unfolding, which takes into account both structural perturbations and association of partially folded intermediates has been elaborated...
  67. ncbi The Saccharomyces cerevisiae nucleoporin Nup2p is a natively unfolded protein
    Daniel P Denning
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    J Biol Chem 277:33447-55. 2002
    ..We address the possible significance of natively unfolded nucleoporins in the mechanics of nucleocytoplasmic trafficking across NPCs...
  68. pmc Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded
    Daniel P Denning
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:2450-5. 2003
    ..The conclusion that FG Nups constitute a family of natively unfolded proteins supports the hypothesis that the FG repeat regions of Nups form a meshwork of random coils at the NPC through which nuclear transport proceeds...
  69. ncbi Structural and functional properties of Yersinia pestis Caf1 capsular antigen and their possible role in fulminant development of primary pneumonic plague
    Vyacheslav M Abramov
    Institute of Immunological Engineering, 142380 Lyubuchany, Moscow Region, Russia
    J Proteome Res 1:307-15. 2002
    ..The specificity of such interaction is confirmed by the inhibition of IL-1alpha binding by Caf1. The Caf1 role in pneumonic plague pathogenesis is discussed...
  70. ncbi Agrin binds alpha-synuclein and modulates alpha-synuclein fibrillation
    I Hsuan Liu
    Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    Glycobiology 15:1320-31. 2005
    ..These findings may indicate shared molecular mechanisms leading to the pathophysiology in these two neurodegenerative disorders...
  71. ncbi The herbicide paraquat causes up-regulation and aggregation of alpha-synuclein in mice: paraquat and alpha-synuclein
    Amy B Manning-Bog
    Parkinson s Institute, Sunnyvale, California 94089, USA
    J Biol Chem 277:1641-4. 2002
    ....

Research Grants16

  1. Molecular Basis of Light Chain Amyloidosis
    ANTHONY FINK; Fiscal Year: 2006
    ..We will screen both a combinatorial peptide library and peptide fragments from light chains as well as some low MW compounds for potential inhibitors of VL fibrillation. ..
  2. The Role of Dopamine and its Analogs in the inhibition*
    ANTHONY FINK; Fiscal Year: 2003
    ..In the long-run this research could provide new strategies for the treatment of Parkinson's disease. ..
  3. THE MOLECULAR BASIS OF ALPHA-SYNUCLEIN AGGREGATION
    ANTHONY FINK; Fiscal Year: 2002
    ....
  4. MECHANISMS OF PROTEIN AGGREGATION AND AMYLOID FORMATION
    ANTHONY FINK; Fiscal Year: 2002
    ..Techniques to be used include mutagenesis, chromatography, kinetics, ATR FTIR spectroscopy, atomic force and electron microscopy, and other biophysical/biochemical methods. ..
  5. Catechol-induced Inhibition of Alpha-synuclein Fibrils
    ANTHONY FINK; Fiscal Year: 2007
    ..In the long-run this research could provide new strategies for the treatment of Parkinson's disease. ..