STANLEY H FIELDS

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi Wide variation in antibiotic resistance proteins identified by functional metagenomic screening of a soil DNA library
    Kelly M McGarvey
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Appl Environ Microbiol 78:1708-14. 2012
  2. ncbi Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins
    Marissa Vignali
    Department of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195, USA
    Malar J 7:211. 2008
  3. ncbi Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins
    Jay R Hesselberth
    Department of Genome Sciences, University of Washington, Box 357730, Seattle, WA 98195, USA
    Genome Biol 7:R30. 2006
  4. ncbi Whole-genome sequencing of a laboratory-evolved yeast strain
    Carlos L Araya
    Department of Genome Sciences, University of Washington, Box 355065, Seattle, Washington 98195, USA
    BMC Genomics 11:88. 2010
  5. ncbi High-throughput two-hybrid analysis. The promise and the peril
    Stanley Fields
    Howard Hughes Medical Institute, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    FEBS J 272:5391-9. 2005
  6. ncbi Functional genomics
    S Fields
    Howard Hughes Medical Institute, Departments of Genetics and Medicine, University of Washington, Box 357360, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 96:8825-6. 1999
  7. ncbi Positioning a medical school for modern biomedical research: the department of genome sciences at the University of Washington School of Medicine
    Stanley Fields
    Genome Sciences and Medicine, University of Washington School of Medicine, Seattle, Washington 98195 7730, USA
    Acad Med 81:882-5. 2006
  8. ncbi Molecular biology. Site-seeing by sequencing
    Stanley Fields
    Departments of Genome Sciences and Medicine, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195-5065, USA
    Science 316:1441-2. 2007
  9. ncbi Interactive learning: lessons from two hybrids over two decades
    Stanley Fields
    Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Proteomics 9:5209-13. 2009
  10. ncbi Cell biology. Whither model organism research?
    Stanley Fields
    Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Science 307:1885-6. 2005

Research Grants

  1. INTERDISCIPLINARY TRAINING IN GENOMIC SCIENCES
    Stanley Fields; Fiscal Year: 2007
  2. INTERDISCIPLINARY TRAINING IN GENOMIC SCIENCES
    Stanley Fields; Fiscal Year: 2007

Collaborators

Detail Information

Publications39

  1. ncbi Wide variation in antibiotic resistance proteins identified by functional metagenomic screening of a soil DNA library
    Kelly M McGarvey
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Appl Environ Microbiol 78:1708-14. 2012
    ..Functional metagenomic screening provides insight into the large variability in antibiotic resistance protein sequences, revealing divergent variants that preserve protein function...
  2. ncbi Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins
    Marissa Vignali
    Department of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195, USA
    Malar J 7:211. 2008
    ..To better understand this aspect of malaria pathogenesis, a study was conducted with the goal of identifying interactions between proteins of the parasite and those of its human host...
  3. ncbi Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins
    Jay R Hesselberth
    Department of Genome Sciences, University of Washington, Box 357730, Seattle, WA 98195, USA
    Genome Biol 7:R30. 2006
    ..Here we built a network composed of WW domain-protein interactions that illuminates novel features of WW domain-containing proteins and their protein interaction partners...
  4. ncbi Whole-genome sequencing of a laboratory-evolved yeast strain
    Carlos L Araya
    Department of Genome Sciences, University of Washington, Box 355065, Seattle, Washington 98195, USA
    BMC Genomics 11:88. 2010
    ..New DNA sequencing technologies now allow the genome of parental and evolved strains of microorganisms to be rapidly determined...
  5. ncbi High-throughput two-hybrid analysis. The promise and the peril
    Stanley Fields
    Howard Hughes Medical Institute, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    FEBS J 272:5391-9. 2005
    ..Two examples are provided of this combination of high-throughput experimentation and computational analysis, focused on the interaction of Plasmodium falciparum proteins and of Saccharomyces cerevisiae membrane proteins...
  6. ncbi Functional genomics
    S Fields
    Howard Hughes Medical Institute, Departments of Genetics and Medicine, University of Washington, Box 357360, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 96:8825-6. 1999
    ..The field of functional genomics seeks to devise and apply technologies that take advantage of the growing body of sequence information to analyze the full complement of genes and proteins encoded by an organism...
  7. ncbi Positioning a medical school for modern biomedical research: the department of genome sciences at the University of Washington School of Medicine
    Stanley Fields
    Genome Sciences and Medicine, University of Washington School of Medicine, Seattle, Washington 98195 7730, USA
    Acad Med 81:882-5. 2006
    ..Lessons learned in crafting this department are also discussed, as well as how these might apply to other medical schools...
  8. ncbi Molecular biology. Site-seeing by sequencing
    Stanley Fields
    Departments of Genome Sciences and Medicine, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195-5065, USA
    Science 316:1441-2. 2007
  9. ncbi Interactive learning: lessons from two hybrids over two decades
    Stanley Fields
    Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Proteomics 9:5209-13. 2009
    ....
  10. ncbi Cell biology. Whither model organism research?
    Stanley Fields
    Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Science 307:1885-6. 2005
  11. ncbi The interplay of biology and technology
    S Fields
    Howard Hughes Medical Institute, Department of Genetics, University of Washington, Box 357360, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 98:10051-4. 2001
    ..Consideration of how methodologies emerge has implications for the planning of interdisciplinary centers and the training of the next generation of scientists...
  12. ncbi Genomics. A crisis in postgenomic nomenclature
    Stanley Fields
    Department of Genome Sciences and Department of Medicine, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Science 296:671-2. 2002
  13. ncbi Sir2-independent life span extension by calorie restriction in yeast
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    PLoS Biol 2:E296. 2004
    ..These findings indicate that Sir2 and calorie restriction act in parallel pathways to promote longevity in yeast and, perhaps, higher eukaryotes...
  14. ncbi Extension of chronological life span in yeast by decreased TOR pathway signaling
    R Wilson Powers
    Department of Genome Sciences and Medicine, The Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Genes Dev 20:174-84. 2006
    ..We propose that up-regulation of a highly conserved response to starvation-induced stress is important for life span extension by decreased TOR signaling in yeast and higher eukaryotes...
  15. ncbi Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4
    Kristan K Steffen
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Cell 133:292-302. 2008
    ..Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms...
  16. ncbi Genome-wide identification of spliced introns using a tiling microarray
    Zhihong Zhang
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Genome Res 17:503-9. 2007
    ..Because the debranching enzyme is conserved among eukaryotes, this approach could be generally applicable for the annotation of eukaryotic genes and the detection of novel and alternative splice forms...
  17. ncbi Forcing interactions as a genetic screen to identify proteins that exert a defined activity
    Michael Devit
    Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Seattle, Washington 98195, USA
    Genome Res 15:560-5. 2005
    ....
  18. ncbi Substrate-specific activation of sirtuins by resveratrol
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 280:17038-45. 2005
    ..In light of these findings, the mechanism accounting for putative longevity effects of resveratrol should be reexamined...
  19. ncbi Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients
    Matt Kaeberlein
    Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA
    Science 310:1193-6. 2005
    ..We propose that the TOR and Sch9 kinases define a primary conduit through which excess nutrient intake limits longevity in yeast...
  20. ncbi Assigning function to yeast proteins by integration of technologies
    Tony R Hazbun
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Mol Cell 12:1353-65. 2003
    ..This combination of technologies, coupled with annotation using GO, is a powerful approach to classifying genes...
  21. ncbi Comment on "HST2 mediates SIR2-independent life-span extension by calorie restriction"
    Matt Kaeberlein
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Science 312:1312; author reply 1312. 2006
    ..Contradictory to this, we find that CR greatly increases life span in cells lacking Sir2, Hst1, and Hst2, which suggests that CR is not mediated by Sir2, Hst2, or Hst1...
  22. ncbi Global mapping of protein-DNA interactions in vivo by digital genomic footprinting
    Jay R Hesselberth
    Department of Genome Sciences, University of Washington, Seattle, USA
    Nat Methods 6:283-9. 2009
    ..Digital genomic footprinting should be a powerful approach to delineate the cis-regulatory framework of any organism with an available genome sequence...
  23. ncbi Increased life span due to calorie restriction in respiratory-deficient yeast
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America
    PLoS Genet 1:e69. 2005
    ....
  24. ncbi A genome-wide screen for site-specific DNA-binding proteins
    Tony R Hazbun
    Department of Genome Sciences, Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 1:538-43. 2002
    ..This report demonstrates the feasibility of identifying DNA binding activities by rapidly assaying a large fraction of the predicted open reading frames of an organism for binding to a regulatory DNA motif...
  25. ncbi High-throughput profiling of amino acids in strains of the Saccharomyces cerevisiae deletion collection
    Sara J Cooper
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Genome Res 20:1288-96. 2010
    ..This approach establishes metabolite profiling as capable of characterizing genes in a large collection of genetic variants...
  26. ncbi Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase
    Christopher L Brett
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 182:1141-51. 2008
    ..We propose that signal propagation through the Ypt7 pathway is controlled by integrated feedback and feed-forward loops. In this model, Yck3 enforces a requirement for the activated Rab in docking and fusion...
  27. ncbi Genes determining yeast replicative life span in a long-lived genetic background
    Matt Kaeberlein
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Mech Ageing Dev 126:491-504. 2005
    ..These results define a limited number of genes likely to regulate replicative life span in a strain-independent manner, and create a basis for future epistasis analysis to determine genetic pathways of aging...
  28. ncbi A protein interaction network of the malaria parasite Plasmodium falciparum
    Douglas J LaCount
    Howard Hughes Medical Institute, University of Washington, Box 357730, Seattle, Washington 98195, USA
    Nature 438:103-7. 2005
    ..These data constitute the first extensive description of the protein interaction network for this important human pathogen...
  29. ncbi Lifespan extension in Caenorhabditis elegans by complete removal of food
    Tammi L Kaeberlein
    Department of Microbiology, University of Washington, Seattle, WA, USA
    Aging Cell 5:487-94. 2006
    ..Removal of bacterial food also increases lifespan when initiated in postreproductive adults, suggesting that dietary restriction started during middle age can result in a substantial longevity benefit that is independent of reproduction...
  30. ncbi A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity
    Anne Claude Gingras
    Institute for Systems Biology, Seattle, Washington 98103, USA
    Mol Cell Proteomics 4:1725-40. 2005
    ..Finally we found that PP4R3 may target PP4cs to the DNA damage repair machinery at least in part via an interaction with Rad53 (CHK2)...
  31. ncbi High-resolution mapping of protein sequence-function relationships
    Douglas M Fowler
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Nat Methods 7:741-6. 2010
    ..Our approach could be applied to many in vitro or in vivo protein assays, providing a general means for understanding how protein function relates to sequence...
  32. ncbi Identification of putative Plasmodium falciparum mefloquine resistance genes
    Mara Jeffress
    Molecular and Cellular Biology Graduate Program, Box 357730, Seattle, WA 98195, USA
    Mol Biochem Parasitol 139:133-9. 2005
    ....
  33. ncbi Selection of yeast strains with enhanced expression of Plasmodium falciparum proteins
    Douglas J LaCount
    Howard Hughes Medical Institute, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Mol Biochem Parasitol 163:119-22. 2009
    ..falciparum genes. These yeast strains may be useful for the analysis and purification of P. falciparum proteins...
  34. ncbi Large-scale identification of yeast integral membrane protein interactions
    John P Miller
    Department of Genome Sciences, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:12123-8. 2005
    ..The results identify potential previously undescribed components of established biological processes and roles for integral membrane proteins of ascribed functions...
  35. ncbi A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010
    ..Finally, we constructed a three-dimensional model of the yeast genome. Our findings provide a glimpse of the interface between the form and function of a eukaryotic genome...
  36. ncbi Suppression of statin effectiveness by copper and zinc in yeast and human cells
    Douglas M Fowler
    Department of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195, USA
    Mol Biosyst 7:533-44. 2011
    ..Because copper and zinc impair the ability of statin to reduce sterol biosynthesis, dietary intake of these metals could have clinical relevance for statin treatment in humans...
  37. ncbi Requirement of an intact microtubule cytoskeleton for aggregation and inclusion body formation by a mutant huntingtin fragment
    Paul J Muchowski
    Department of Genome Sciences University of Washington, Box 357730, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 99:727-32. 2002
    ....
  38. ncbi Structural genomics of pathogenic protozoa: an overview
    Erkang Fan
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Methods Mol Biol 426:497-513. 2008
    ..quot;Fragment cocktail crystallography" for medical structural genomics is also described...
  39. ncbi Binding specificity of Toll-like receptor cytoplasmic domains
    Victoria Brown
    Department of Genome Sciences, University of Washington, Seattle 98195, USA
    Eur J Immunol 36:742-53. 2006
    ..In summary, interactions between the TLR, MyD88, and novel associated proteins have been characterized...

Research Grants2

  1. INTERDISCIPLINARY TRAINING IN GENOMIC SCIENCES
    Stanley Fields; Fiscal Year: 2007
    ....
  2. INTERDISCIPLINARY TRAINING IN GENOMIC SCIENCES
    Stanley Fields; Fiscal Year: 2007
    ..abstract_text> ..