P J Fay

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc Acidic residues C-terminal to the A2 domain facilitate thrombin-catalyzed activation of factor VIII
    Jennifer L Newell
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 47:8786-95. 2008
  2. ncbi request reprint Mutating factor VIII: lessons from structure to function
    Philip J Fay
    Department of Biochemistry, School of Medicine, University of Rochester, P O Box 712, 601 Elmwood Ave, Rochester, NY 14642, USA
    Blood Rev 19:15-27. 2005
  3. pmc Contribution of factor VIII light-chain residues 2007-2016 to an activated protein C-interactive site
    Masahiro Takeyama
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Thromb Haemost 109:187-98. 2013
  4. ncbi request reprint The A1 and A2 subunits of factor VIIIa synergistically stimulate factor IXa catalytic activity
    P J Fay
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 274:15401-6. 1999
  5. ncbi request reprint Cleavage of factor VIII heavy chain is required for the functional interaction of a2 subunit with factor IXA
    P J Fay
    Department of Biochemistry and Biophysics and the Department of Medicine, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 276:12434-9. 2001
  6. ncbi request reprint Human inhibitor antibodies specific for the factor VIII A2 domain disrupt the interaction between the subunit and factor IXa
    P J Fay
    Department of Biochemistry, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 274:29826-30. 1999
  7. ncbi request reprint Factor VIIIa cofactor activity shows enhanced ionic strength sensitivity in the absence of phospholipid
    P J Fay
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, NY 14642, USA
    Biochim Biophys Acta 1548:159-68. 2001
  8. ncbi request reprint Activation of factor VIII and mechanisms of cofactor action
    Philip J Fay
    Departments of Biochemistry and Biophysics and Medicine, PO Box 712, University of Rochester School of Medicine, Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Blood Rev 18:1-15. 2004
  9. ncbi request reprint The A2 subunit of factor VIIIa modulates the active site of factor IXa
    P J Fay
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 273:19049-54. 1998
  10. ncbi request reprint Factor VIII structure and function
    Philip J Fay
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, New York 14642, USA
    Int J Hematol 83:103-8. 2006

Collaborators

  • Q Zhou
  • Yan Chen
  • ROBERT BAMBARA
  • W Wang
  • S P Bajaj
  • D Scandella
  • PETER NEWTON WALSH
  • T Myles
  • Alireza R Rezaie
  • Hironao Wakabayashi
  • Keiji Nogami
  • Jennifer L Newell
  • Fatbardha Varfaj
  • H Wakabayashi
  • Charles Ansong
  • P Vincent Jenkins
  • A E Griffiths
  • Jan Freas
  • Masahiro Takeyama
  • Chandrashekhara Manithody
  • Indu Jagannathan
  • Kyla M Schmidt
  • C Ansong
  • P V Jenkins
  • Kyla Schmidt
  • Michele Wisniewski
  • Mary E Koszelak Rosenblum
  • M Balakrishnan
  • F K Hagen
  • Amy E Griffiths
  • H Travis Ichikawa
  • Tricia Kruger
  • Jennifer Deangelis
  • F Varfaj
  • K A Lapan
  • Stephen M Miles
  • S M Miles
  • Stephen Miles
  • Julie Neuberg
  • Lawrence L K Leung
  • Syed S Ahmad
  • Ya Chi Su
  • Julie L Dill
  • J L Dill
  • Kirsty A Lapan
  • Zhu Zhen
  • Mini Balakrishnan
  • Chockalingam Palaniappan
  • Maria Mastri
  • Bernard P Roques
  • G M Fuentes
  • M E Koszelak
  • M Mastri
  • J J DeStefano
  • R G Buiser
  • L M Mallaber
  • T W Myers

Detail Information

Publications51

  1. pmc Acidic residues C-terminal to the A2 domain facilitate thrombin-catalyzed activation of factor VIII
    Jennifer L Newell
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 47:8786-95. 2008
    ..These results suggest that residues Glu720, Asp721, Glu724, and Asp725 likely constitute an exosite-interactive region in factor VIII facilitating cleavages for procofactor activation...
  2. ncbi request reprint Mutating factor VIII: lessons from structure to function
    Philip J Fay
    Department of Biochemistry, School of Medicine, University of Rochester, P O Box 712, 601 Elmwood Ave, Rochester, NY 14642, USA
    Blood Rev 19:15-27. 2005
    ..In this review, we document important and novel contributions following this line of investigation...
  3. pmc Contribution of factor VIII light-chain residues 2007-2016 to an activated protein C-interactive site
    Masahiro Takeyama
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Thromb Haemost 109:187-98. 2013
    ..These results show a contribution of a number of residues within the 2007-2016 sequence, and in particular residues Met2010, Ser2011, and Leu2013 to an APC-interactive site...
  4. ncbi request reprint The A1 and A2 subunits of factor VIIIa synergistically stimulate factor IXa catalytic activity
    P J Fay
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 274:15401-6. 1999
    ....
  5. ncbi request reprint Cleavage of factor VIII heavy chain is required for the functional interaction of a2 subunit with factor IXA
    P J Fay
    Department of Biochemistry and Biophysics and the Department of Medicine, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 276:12434-9. 2001
    ..These results indicate that HC cleavage by either thrombin or factor Xa is essential to expose the factor IXa-interactive site(s) in the A2 subunit required to modulate protease activity...
  6. ncbi request reprint Human inhibitor antibodies specific for the factor VIII A2 domain disrupt the interaction between the subunit and factor IXa
    P J Fay
    Department of Biochemistry, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 274:29826-30. 1999
    ..Furthermore, these results also suggest that factor VIII residues 484-509 contribute to a factor IXa-interactive site...
  7. ncbi request reprint Factor VIIIa cofactor activity shows enhanced ionic strength sensitivity in the absence of phospholipid
    P J Fay
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, NY 14642, USA
    Biochim Biophys Acta 1548:159-68. 2001
    ....
  8. ncbi request reprint Activation of factor VIII and mechanisms of cofactor action
    Philip J Fay
    Departments of Biochemistry and Biophysics and Medicine, PO Box 712, University of Rochester School of Medicine, Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Blood Rev 18:1-15. 2004
    ..This effect is manifested in a dramatic increase in the catalytic rate constant, k(cat), by mechanisms that remain poorly understood...
  9. ncbi request reprint The A2 subunit of factor VIIIa modulates the active site of factor IXa
    P J Fay
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 273:19049-54. 1998
    ..These results indicate that the isolated A2 subunit modulates the active site of factor IXa and identifies a functional role for this subunit in factor VIIIa...
  10. ncbi request reprint Factor VIII structure and function
    Philip J Fay
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, New York 14642, USA
    Int J Hematol 83:103-8. 2006
    ..Mechanisms for the down-regulation of factor Xase focus upon inactivation of the cofactor and include dissociation of the A2 subunit as well as activated protein C-catalyzed proteolysis...
  11. ncbi request reprint Metal ion-independent association of factor VIII subunits and the roles of calcium and copper ions for cofactor activity and inter-subunit affinity
    H Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Biochemistry 40:10293-300. 2001
    ..Ca(2+) appears essential to promote the active conformation of factor VIII while Cu(2+) primarily enhances the intersubunit affinity...
  12. ncbi request reprint Polymerization and RNase H activities of the reverse transcriptases from avian myeloblastosis, human immunodeficiency, and Moloney murine leukemia viruses are functionally uncoupled
    J J DeStefano
    Department of Biochemistry, University of Rochester, New York 14642
    J Biol Chem 266:7423-31. 1991
    ..Results demonstrate that the RNase H function is much less active than the polymerization function during processive DNA synthesis and that the activities are not strictly coupled...
  13. ncbi request reprint Mutations associated with hemophilia A in the 558-565 loop of the factor VIIIa A2 subunit alter the catalytic activity of the factor Xase complex
    P Vincent Jenkins
    Department of Biochemistry and Biophysics and the Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
    Blood 100:501-8. 2002
    ..These results show that residues within the 558-565 loop are critical in modulating FIXa enzymatic activity but do not contribute significantly to the affinity of FVIIIa for FIXa...
  14. pmc Use of affinity-directed liquid chromatography-mass spectrometry to map the epitopes of a factor VIII inhibitor antibody fraction
    A E Griffiths
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY, USA
    J Thromb Haemost 9:1534-40. 2011
    ..Neutralizing factor (F) VIII antibodies develop in approximately 30% of individuals with hemophilia A and show specificity to multiple sites in the FVIII protein...
  15. ncbi request reprint Requirements for the catalysis of strand transfer synthesis by retroviral DNA polymerases
    R G Buiser
    Department of Biochemistry, University of Rochester, New York 14642
    J Biol Chem 266:13103-9. 1991
    ..These results suggest that strand transfer synthesis relies on an unidentified functional activity present in RTs...
  16. ncbi request reprint Factor IXa:factor VIIIa interaction. helix 330-338 of factor ixa interacts with residues 558-565 and spatially adjacent regions of the a2 subunit of factor VIIIa
    S P Bajaj
    Department of Medicine, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 276:16302-9. 2001
    ..These data support a conclusion that the helix 330 of factor IXa interacts with the A2 558-565 sequence. This information was used to model the interface between the IXa protease domain and the A2 subunit, which is also provided herein...
  17. ncbi request reprint Residues 110-126 in the A1 domain of factor VIII contain a Ca2+ binding site required for cofactor activity
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Biol Chem 279:12677-84. 2004
    ....
  18. ncbi request reprint Clustered basic residues within segment 484-510 of the factor VIIIa A2 subunit contribute to the catalytic efficiency for factor Xa generation
    P V Jenkins
    Department of Biochemistry, University of Rochester Medical Center, Rochester, NY, USA
    J Thromb Haemost 2:452-8. 2004
    ....
  19. pmc Combining mutations of charged residues at the A2 domain interface enhances factor VIII stability over single point mutations
    H Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA
    J Thromb Haemost 7:438-44. 2009
    ..Blood, 112, 2761, 2008)...
  20. pmc Cleavage at Arg-1689 influences heavy chain cleavages during thrombin-catalyzed activation of factor VIII
    Jennifer L Newell
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 284:11080-9. 2009
    ..Overall, these results are consistent with a competition between heavy and light chains for thrombin exosite binding and subsequent proteolysis with binding of the former chain preferred...
  21. ncbi request reprint Contribution of factor VIIIa A2 and A3-C1-C2 subunits to the affinity for factor IXa in factor Xase
    P Vincent Jenkins
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 43:5094-101. 2004
    ..Thus both A2 and A3-C1-C2 subunits contribute to the affinity of FVIIIa for FIXa in the membrane-dependent FXase...
  22. ncbi request reprint Cofactor activities of factor VIIIa and A2 subunit following cleavage of A1 subunit at Arg336
    Mary E Koszelak Rosenblum
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 277:11664-9. 2002
    ....
  23. ncbi request reprint Identification of a factor Xa-interactive site within residues 337-372 of the factor VIII heavy chain
    Keiji Nogami
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 279:15763-71. 2004
    ....
  24. pmc A Glu113Ala mutation within a factor VIII Ca2+-binding site enhances cofactor interactions in factor Xase
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Biochemistry 44:10298-304. 2005
    ....
  25. ncbi request reprint Epitope mapping factor VIII A2 domain by affinity-directed mass spectrometry: residues 497-510 and 584-593 comprise a discontinuous epitope for the monoclonal antibody R8B12
    C Ansong
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine, Rochester, NY 14642, USA
    J Thromb Haemost 4:842-7. 2006
    ..Furthermore, based upon blotting specificity, we speculate that residues 584-593 make a substantially greater contribution to the binding energy for this interaction...
  26. pmc Thrombin-catalyzed activation of factor VIII with His substituted for Arg372 at the P1 site
    Keiji Nogami
    Department of Biochemistry and Biophysics, PO Box 712, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Blood 105:4362-8. 2005
    ..These results indicate a markedly reduced rate of cleavage following substitution at the P(1)Arg, and this property likely reflects the severity of the hemophilia A phenotype...
  27. ncbi request reprint Activated protein C-catalyzed inactivation of human factor VIII and factor VIIIa. Identification of cleavage sites and correlation of proteolysis with cofactor activity
    P J Fay
    Department of Medicine, University of Rochester School of Medicine and Dentistry, New York 14642
    J Biol Chem 266:20139-45. 1991
    ..These results suggest a mechanism for activated protein C-catalyzed inactivation of factor VIII(alpha) involving both covalent alteration and fragment dissociation...
  28. ncbi request reprint Interaction of the A1 subunit of factor VIIIa and the serine protease domain of factor X identified by zero-length cross-linking
    K A Lapan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry Rochester, New York, USA
    Thromb Haemost 80:418-22. 1998
    ....
  29. ncbi request reprint A3 domain residue Glu1829 contributes to A2 subunit retention in factor VIIIa
    H Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA
    J Thromb Haemost 5:996-1001. 2007
    ..A primary reason for the instability of FVIIIa is the tendency for the A2 subunit to dissociate from FVIIIa leading to an inactive cofactor and consequent loss of FXase activity...
  30. ncbi request reprint The kissing hairpin sequence promotes recombination within the HIV-I 5' leader region
    M Balakrishnan
    Department of Biochemistry and Biophysics and Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 276:36482-92. 2001
    ..Overall, these results suggest that template dimerization facilitated by the unique geometry of the DIS-promoted kissing interactions effectively promotes recombination within the HIV-I 5'-UTR...
  31. pmc Identification of residues contributing to A2 domain-dependent structural stability in factor VIII and factor VIIIa
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642
    J Biol Chem 283:11645-51. 2008
    ..This observation is consistent with an altered conformation involving new inter-subunit interactions involving A2 domain following procofactor activation...
  32. pmc Factor VIII lacking the C2 domain retains cofactor activity in vitro
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 285:25176-84. 2010
    ..Together, these results indicate that FVIII domains other than C2, likely C1, make significant contributions to membrane-binding and membrane-dependent function...
  33. ncbi request reprint Exosite-interactive regions in the A1 and A2 domains of factor VIII facilitate thrombin-catalyzed cleavage of heavy chain
    Keiji Nogami
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 280:18476-87. 2005
    ..These data suggest the acidic region comprising residues 389-394 in factor VIII A2 domain interacts with thrombin via its heparin-binding exosite and facilitates cleavage at Arg(740) during procofactor activation...
  34. pmc Relationship between plus strand DNA synthesis removal of downstream segments of RNA by human immunodeficiency virus, murine leukemia virus and avian myeloblastoma virus reverse transcriptases
    G M Fuentes
    Department of Microbiology, University of Rochester, School of Medicine and Dentistry, NY 14642, USA
    Nucleic Acids Res 24:1719-26. 1996
    ..This suggests that degradation of downstream pieces of RNA is not absolutely required before synthesis of the plus strand DNA. The implications of these findings for viral replication are discussed...
  35. ncbi request reprint Proteolysis at Arg740 facilitates subsequent bond cleavages during thrombin-catalyzed activation of factor VIII
    Jennifer L Newell
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 282:25367-75. 2007
    ..Overall, these results support a model whereby cleavage of factor VIII by thrombin is an ordered pathway with cleavage at Arg740 facilitating cleavages at Arg372 and Arg1689, which result in procofactor activation...
  36. ncbi request reprint Altered interactions between the A1 and A2 subunits of factor VIIIa following cleavage of A1 subunit by factor Xa
    Keiji Nogami
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 278:1634-41. 2003
    ..Furthermore, the C terminus of A1 contributes to the K(m) for factor X binding to factor Xase, and this parameter is critical for activity assessed in plasma-based assays...
  37. ncbi request reprint Ca(2+) binding to both the heavy and light chains of factor VIII is required for cofactor activity
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 41:8485-92. 2002
    ..These results suggest that factor VIII contains two Ca(2+) binding sites with different affinities and that active factor VIII can be reconstituted from HC and LC only when both chains are preactivated by Ca(2+)...
  38. pmc pH-dependent association of factor VIII chains: enhancement of affinity at physiological pH by Cu2+
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Ave, Rochester, NY 14642, USA
    Biochim Biophys Acta 1764:1094-101. 2006
    ..Furthermore, copper ion contributes to the maintenance of the heterodimer at physiologic pH by a mechanism consistent with bridging the two chains...
  39. pmc Identification of residues in the 558-loop of factor VIIIa A2 subunit that interact with factor IXa
    Indu Jagannathan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 284:32248-55. 2009
    ..Furthermore, variants Y555A, V559A, D560A, G563A, I566A, and D569A showed >80% reduction in k(cat) for factor Xa generation. These results identify residues in the 558-loop critical to interaction with factor IXa in Xase...
  40. pmc Role of P1 residues Arg336 and Arg562 in the activated-Protein-C-catalysed inactivation of Factor VIIIa
    Fatbardha Varfaj
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochem J 396:355-62. 2006
    ..Thus, while P1 residues contribute to catalytic efficiency, residues removed from these sites make a primary contribution to the overall binding of APC to Factor VIIIa...
  41. ncbi request reprint Mn2+ binding to factor VIII subunits and its effect on cofactor activity
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York, 14642, USA
    Biochemistry 42:145-53. 2003
    ..Thus, Mn2+appears to generate factor VIII cofactor activity by a similar mechanism as observed for Ca2+following its association at nonidentical sites on the protein...
  42. pmc Generation of enhanced stability factor VIII variants by replacement of charged residues at the A2 domain interface
    Hironao Wakabayashi
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, NY, USA
    Blood 112:2761-9. 2008
    ..These results indicate that replacement of buried charged residues is an effective alternative to covalent modification in increasing factor VIII (VIIIa) stability...
  43. ncbi request reprint Factor VIII A3 domain residues 1954-1961 represent an A1 domain-interactive site
    Charles Ansong
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 44:8850-7. 2005
    ..We propose that residues 1954-1961 of the A3 domain contribute to interactions with the A1 domain, facilitating their association in factor VIII...
  44. ncbi request reprint Residues surrounding Arg336 and Arg562 contribute to the disparate rates of proteolysis of factor VIIIa catalyzed by activated protein C
    Fatbardha Varfaj
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Biol Chem 282:20264-72. 2007
    ..Efficient cleavage at Arg(336) by APC is attributed to favorable P4-P3' residues at this site, whereas cleavage at Arg(562) can be accelerated following replacement with more optimal P4-P3' residues...
  45. ncbi request reprint Mechanisms of factor Xa-catalyzed cleavage of the factor VIIIa A1 subunit resulting in cofactor inactivation
    Keiji Nogami
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 278:16502-9. 2003
    ..Furthermore, cleavage at Lys(36) appears to be selectively modulated by the C-terminal acidic region of A1, a region that may interact with factor Xa via its heparin-binding exosite...
  46. ncbi request reprint Mechanisms of interactions of factor X and factor Xa with the acidic region in the factor VIII A1 domain
    Keiji Nogami
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 279:33104-13. 2004
    ..Furthermore, factor Xa but not factor X interacts with high affinity at this site via residues contained within the heparin-binding exosite of the proteinase...
  47. ncbi request reprint Localization of a pH-dependent, A2 subunit-interactive surface within the factor VIIIa A1 subunit
    Keiji Nogami
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, P O Box 712, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochim Biophys Acta 1701:25-35. 2004
    ..This interactive surface appears conformationally labile in the truncated A1 as judged by its apparent stabilization following association with A3-C1-C2...
  48. ncbi request reprint Substrate requirements for secondary cleavage by HIV-1 reverse transcriptase RNase H
    Michele Wisniewski
    Department of Biochemistry and Biophysics and the Cancer Center, University of Rochester, Rochester, New York 14642, USA
    J Biol Chem 277:28400-10. 2002
    ..However, NC protein greatly facilitated the secondary cut on the blunt-ended substrate, suggesting that NC compensates for the unfavorable substrate structure...
  49. ncbi request reprint Mechanism of minus strand strong stop transfer in HIV-1 reverse transcription
    Yan Chen
    Department of Biochemistry and Biophysics, University of Rochester, New York 14642, USA
    J Biol Chem 278:8006-17. 2003
    ..The acceptor invades at gaps created by reverse transcriptase-RNase H in the donor-cDNA hybrid. The fragmented donor is eventually strand-displaced by the acceptor, completing the transfer...
  50. ncbi request reprint Factor VIII A1 domain residues 97-105 represent a light chain-interactive site
    Charles Ansong
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, New York 14642, USA
    Biochemistry 45:13140-9. 2006
    ..These results indicate that residues within segment 97-105 of the A1 domain interact with residues within the A3C1C2 domains of the light chain and contribute to the interface in the factor VIII heterodimer...
  51. ncbi request reprint Exosite-dependent regulation of factor VIIIa by activated protein C
    Chandrashekhara Manithody
    Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, MO 63104, USA
    Blood 101:4802-7. 2003
    ....

Research Grants29

  1. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 2005
    ..Definition of these issues will yield valuable insights into the biochemistry of the native as well as dysfunctional factor VIII molecules, and provide information for the design of superior therapeutics. ..
  2. Factor Vllla interactions in the intrinsic factor Xase
    Philip Fay; Fiscal Year: 2007
    ..Definition of these issues will yield valuable and fundamental insights into the biochemistry of the native as well as dysfunctional factor VIII molecules, and provide information for the design of superior therapeutics ..
  3. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 2007
    ....
  4. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 2009
    ....
  5. Factor VIIIa interactions in the intrinsic factor Xase
    Philip J Fay; Fiscal Year: 2010
    ..In this application we will elucidate fine point structural details of inter-protein interactions that will define mechanisms for factor VIIIa cofactor function and the regulation of its activity in factor Xase. ..
  6. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 1993
    ..protection and the role of protein S in this reaction. Overall, these studies will offer insights into a coagulation protein central to hemostasis...
  7. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 1992
    ....
  8. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip Fay; Fiscal Year: 2001
    ..Definition of these issues will yield valuable insights into the biochemistry of the native as well as dysfunctional factor VIII molecules, and provide information for the design of superior therapeutics. ..
  9. STRUCTURE AND REGULATION OF HUMAN FACTOR VIII
    Philip J Fay; Fiscal Year: 2010
    ....