R V Farese

Summary

Affiliation: University of South Florida
Country: USA

Publications

  1. ncbi request reprint Function and dysfunction of aPKC isoforms for glucose transport in insulin-sensitive and insulin-resistant states
    Robert V Farese
    Department of Internal Medicine, University of South Florida College of Medicine and James A Haley Veterans Administration Medical Center, Tampa, Florida 33612, USA
    Am J Physiol Endocrinol Metab 283:E1-11. 2002
  2. ncbi request reprint Atypical protein kinase C in insulin action and insulin resistance
    R V Farese
    Research Service, James A Haley Veterans Administration Medical Center, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Biochem Soc Trans 33:350-3. 2005
  3. ncbi request reprint Insulin-sensitive protein kinases (atypical protein kinase C and protein kinase B/Akt): actions and defects in obesity and type II diabetes
    Robert V Farese
    James A Haley Veterans Administration Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Exp Biol Med (Maywood) 230:593-605. 2005
  4. ncbi request reprint Protein kinase C-zeta and phosphoinositide-dependent protein kinase-1 are required for insulin-induced activation of ERK in rat adipocytes
    M P Sajan
    J. A. Haley Veterans Hospital Research Service, Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:30495-500. 1999
  5. ncbi request reprint Evidence for involvement of protein kinase C (PKC)-zeta and noninvolvement of diacylglycerol-sensitive PKCs in insulin-stimulated glucose transport in L6 myotubes
    G Bandyopadhyay
    J. A. Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Endocrinology 138:4721-31. 1997
  6. ncbi request reprint Protein kinase C-zeta as a downstream effector of phosphatidylinositol 3-kinase during insulin stimulation in rat adipocytes. Potential role in glucose transport
    M L Standaert
    J A Haley Veterans Hospital Research Service and Departments of Internal Medicine and Biochemistry Molecular Biology, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 272:30075-82. 1997
  7. doi request reprint Role of atypical protein kinase C in activation of sterol regulatory element binding protein-1c and nuclear factor kappa B (NFkappaB) in liver of rodents used as a model of diabetes, and relationships to hyperlipidaemia and insulin resistance
    M P Sajan
    Research Service, James A Haley Veterans Hospital, Tampa, FL 33612, USA
    Diabetologia 52:1197-207. 2009
  8. ncbi request reprint Okadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:14074-8. 1999
  9. ncbi request reprint Chronic activation of protein kinase C in soleus muscles and other tissues of insulin-resistant type II diabetic Goto-Kakizaki (GK), obese/aged, and obese/Zucker rats. A mechanism for inhibiting glycogen synthesis
    A Avignon
    J A Haley Veterans Hospital, Tampa, Florida 33612, USA
    Diabetes 45:1396-404. 1996
  10. ncbi request reprint Rosiglitazone, insulin treatment, and fasting correct defective activation of protein kinase C-zeta/lambda by insulin in vastus lateralis muscles and adipocytes of diabetic rats
    Y Kanoh
    J. A. Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine Tampa, Florida 33612, USA
    Endocrinology 142:1595-605. 2001

Collaborators

Detail Information

Publications73

  1. ncbi request reprint Function and dysfunction of aPKC isoforms for glucose transport in insulin-sensitive and insulin-resistant states
    Robert V Farese
    Department of Internal Medicine, University of South Florida College of Medicine and James A Haley Veterans Administration Medical Center, Tampa, Florida 33612, USA
    Am J Physiol Endocrinol Metab 283:E1-11. 2002
    ..This postulation is of critical importance, as it now appears that insulin-stimulated aPKC activation is compromised in various states of insulin resistance...
  2. ncbi request reprint Atypical protein kinase C in insulin action and insulin resistance
    R V Farese
    Research Service, James A Haley Veterans Administration Medical Center, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Biochem Soc Trans 33:350-3. 2005
    ..Thus increased activation of liver aPKC in hyperinsulinaemic states may contribute significantly to the development of hyperlipidaemia in insulin-resistant states...
  3. ncbi request reprint Insulin-sensitive protein kinases (atypical protein kinase C and protein kinase B/Akt): actions and defects in obesity and type II diabetes
    Robert V Farese
    James A Haley Veterans Administration Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Exp Biol Med (Maywood) 230:593-605. 2005
    ..The present review will attempt to summarize what we believe are some of the more important advances...
  4. ncbi request reprint Protein kinase C-zeta and phosphoinositide-dependent protein kinase-1 are required for insulin-induced activation of ERK in rat adipocytes
    M P Sajan
    J. A. Haley Veterans Hospital Research Service, Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:30495-500. 1999
    ..Our findings therefore suggested that PDK-1 and PKC-zeta serve as a downstream effectors of PI3K, and act in conjunction with GRB2, SOS, RAS, and RAF, to activate MEK and ERK during insulin action in rat adipocytes...
  5. ncbi request reprint Evidence for involvement of protein kinase C (PKC)-zeta and noninvolvement of diacylglycerol-sensitive PKCs in insulin-stimulated glucose transport in L6 myotubes
    G Bandyopadhyay
    J. A. Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Endocrinology 138:4721-31. 1997
    ..Collectively, our findings suggest that, whereas PKC-zeta is a reasonable candidate to participate in insulin stimulation of glucose transport, DAG-sensitive PKCs are unlikely participants...
  6. ncbi request reprint Protein kinase C-zeta as a downstream effector of phosphatidylinositol 3-kinase during insulin stimulation in rat adipocytes. Potential role in glucose transport
    M L Standaert
    J A Haley Veterans Hospital Research Service and Departments of Internal Medicine and Biochemistry Molecular Biology, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 272:30075-82. 1997
    ..Our findings suggest that insulin activates PKC-zeta through PI 3-kinase, and PKC-zeta may act as a downstream effector of PI 3-kinase and contribute to the activation of GLUT4 translocation...
  7. doi request reprint Role of atypical protein kinase C in activation of sterol regulatory element binding protein-1c and nuclear factor kappa B (NFkappaB) in liver of rodents used as a model of diabetes, and relationships to hyperlipidaemia and insulin resistance
    M P Sajan
    Research Service, James A Haley Veterans Hospital, Tampa, FL 33612, USA
    Diabetologia 52:1197-207. 2009
    ....
  8. ncbi request reprint Okadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:14074-8. 1999
    ..Our findings suggest that, as with insulin, PI 3-kinase-dependent atypical PKCs, zeta and lambda, are required for okadaic acid-induced increases in GLUT4 translocation and glucose transport in rat adipocytes and 3T3/L1 adipocytes...
  9. ncbi request reprint Chronic activation of protein kinase C in soleus muscles and other tissues of insulin-resistant type II diabetic Goto-Kakizaki (GK), obese/aged, and obese/Zucker rats. A mechanism for inhibiting glycogen synthesis
    A Avignon
    J A Haley Veterans Hospital, Tampa, Florida 33612, USA
    Diabetes 45:1396-404. 1996
    ....
  10. ncbi request reprint Rosiglitazone, insulin treatment, and fasting correct defective activation of protein kinase C-zeta/lambda by insulin in vastus lateralis muscles and adipocytes of diabetic rats
    Y Kanoh
    J. A. Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine Tampa, Florida 33612, USA
    Endocrinology 142:1595-605. 2001
    ..thiazolidinediones, chronic insulin treatment, and short-term fasting, similarly improve defects in insulin-stimulated glucose transport at least partly by correcting defects in insulin-induced activation of PKC-zeta/lambda...
  11. ncbi request reprint Metformin improves atypical protein kinase C activation by insulin and phosphatidylinositol-3,4,5-(PO4)3 in muscle of diabetic subjects
    V Luna
    ACOS 151, James A Haley Veterans Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Diabetologia 49:375-82. 2006
    ..g., aminoimidazole-4-carboxamide-1-beta-D: -riboside (AICAR), simultaneously activate atypical protein kinase C (aPKC), which appears to be required for the glucose transport effects of AICAR and insulin...
  12. doi request reprint Combined thiazolidinedione-metformin treatment synergistically improves insulin signalling to insulin receptor substrate-1-dependent phosphatidylinositol 3-kinase, atypical protein kinase C and protein kinase B/Akt in human diabetic muscle
    N Temofonte
    Research Service, James A Haley Veterans Administration Medical Center, Tampa, FL 33612, USA
    Diabetologia 52:60-4. 2009
    ..However, effects of combined thiazolidinedione-metformin treatment on aPKC or PKB activation by sub-maximal and maximal insulin are unknown...
  13. ncbi request reprint Insulin activates protein kinases C-zeta and C-lambda by an autophosphorylation-dependent mechanism and stimulates their translocation to GLUT4 vesicles and other membrane fractions in rat adipocytes
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:25308-16. 1999
    ....
  14. ncbi request reprint Insulin and PIP3 activate PKC-zeta by mechanisms that are both dependent and independent of phosphorylation of activation loop (T410) and autophosphorylation (T560) sites
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Biochemistry 40:249-55. 2001
    ....
  15. pmc AICAR and metformin, but not exercise, increase muscle glucose transport through AMPK-, ERK-, and PDK1-dependent activation of atypical PKC
    M P Sajan
    Research Service, James A Haley Veterans Hospital and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Am J Physiol Endocrinol Metab 298:E179-92. 2010
    ..On the other hand, although aPKC is activated by treadmill exercise, this activation is not required for exercise-induced increases in glucose transport, and therefore may be a redundant mechanism...
  16. ncbi request reprint Activation of protein kinase C (alpha, beta, and zeta) by insulin in 3T3/L1 cells. Transfection studies suggest a role for PKC-zeta in glucose transport
    G Bandyopadhyay
    J. A. Haley Veterans Hospital Research Service, and Departments of Internal Medicine and Biochemistry, University of South Florida, Tampa, Florida 33612, USA
    J Biol Chem 272:2551-8. 1997
    ..These findings suggested that: (a) insulin activates PKC-zeta, as well as PKC-alpha and beta; and (b) PKC-zeta is required for, and may contribute to, insulin effects on glucose transport in 3T3/L1 cells...
  17. ncbi request reprint Insulin-sensitive phospholipid signaling systems and glucose transport. Update II
    R V Farese
    JA Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Exp Biol Med (Maywood) 226:283-95. 2001
    ..PKB also appears to be the major regulator of glycogen synthase. Together, atypical PKCs and PKB serve as a potent, integrated PI3K/PDK-1-directed signaling system that is used by insulin to regulate glucose metabolism...
  18. ncbi request reprint Contrasting insulin dose-dependent defects in activation of atypical protein kinase C and protein kinase B/Akt in muscles of obese diabetic humans
    L Casaubon
    Research Service, James A Haley Veterans Administration Medical Center and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL, USA
    Diabetologia 49:3000-8. 2006
    ..The aim of this study was to test the hypothesis that dose-related effects of insulin may account for the reported differences in insulin signalling to PKB in diabetic muscle...
  19. ncbi request reprint Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation
    G Bandyopadhyay
    J A Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 276:35537-45. 2001
    ..Our findings suggest the operation of a novel pathway for activating PKC-zeta/lambda and glucose transport...
  20. ncbi request reprint Impaired activation of protein kinase C-zeta by insulin and phosphatidylinositol-3,4,5-(PO4)3 in cultured preadipocyte-derived adipocytes and myotubes of obese subjects
    M P Sajan
    Research Service, James A Haley Hospital Veterans Hospital, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Clin Endocrinol Metab 89:3994-8. 2004
    ..These findings suggest the presence of defects in PI3K and aPKC activation that persist in cultured cells and limit insulin-stimulated glucose transport in adipocytes and myocytes of obese subjects...
  21. ncbi request reprint Effects of knockout of the protein kinase C beta gene on glucose transport and glucose homeostasis
    M L Standaert
    J A Haley Veterans Hospital Research Service, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Endocrinology 140:4470-7. 1999
    ....
  22. pmc Insulin signalling in hepatocytes of humans with type 2 diabetes: excessive production and activity of protein kinase C-ι (PKC-ι) and dependent processes and reversal by PKC-ι inhibitors
    M P Sajan
    Medical and Research Services, James A Haley Veterans Medical Center, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Diabetologia 55:1446-57. 2012
    ....
  23. pmc Insulin increases mRNA levels of protein kinase C-alpha and -beta in rat adipocytes and protein kinase C-alpha, -beta and -theta in rat skeletal muscle
    A Avignon
    Department of Internal Medicine and Biochemistry, J A Haley Veterans Hospital, University of South Florida College of Medicine, Tampa 33612, USA
    Biochem J 308:181-7. 1995
    ..This mechanism of increasing mRNA may be important in maintaining PKC levels during the continued action of insulin...
  24. ncbi request reprint PKC-zeta mediates insulin effects on glucose transport in cultured preadipocyte-derived human adipocytes
    Gautam Bandyopadhyay
    J A Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Clin Endocrinol Metab 87:716-23. 2002
    ..Our findings provide convincing evidence that aPKCs and upstream activators, PI 3-kinase and PDK-1, play important roles in insulin-stimulated glucose transport in preadipocyte-derived human adipocytes...
  25. ncbi request reprint Tissue-specific differences in activation of atypical protein kinase C and protein kinase B in muscle, liver, and adipocytes of insulin receptor substrate-1 knockout mice
    Mini P Sajan
    Research Service, James A Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Mol Endocrinol 18:2513-21. 2004
    ..These complexities in aPKC and PKB activation may be relevant to metabolic abnormalities seen in tissues in which IRS-1 or IRS-2 is specifically or predominantly down-regulated...
  26. pmc Differential effect of bicycling exercise intensity on activity and phosphorylation of atypical protein kinase C and extracellular signal-regulated protein kinase in skeletal muscle
    Erik A Richter
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sports Sciences, University of Copenhagen, 13 Universitetsparken, DK 2100 Copenhagen, Denmark
    J Physiol 560:909-18. 2004
    ..In addition, increase in muscle activity of aPKC or ERK1/2 during exercise is not closely related to energy demands of the muscle but may serve other regulatory or permissive functions in muscle...
  27. ncbi request reprint Requirements for pYXXM motifs in Cbl for binding to the p85 subunit of phosphatidylinositol 3-kinase and Crk, and activation of atypical protein kinase C and glucose transport during insulin action in 3T3/L1 adipocytes
    Mary L Standaert
    Research Service, James A Haley Veterans Medical Center, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Biochemistry 43:15494-502. 2004
    ....
  28. ncbi request reprint Insulin substrates 1 and 2 are corequired for activation of atypical protein kinase C and Cbl-dependent phosphatidylinositol 3-kinase during insulin action in immortalized brown adipocytes
    Atsushi Miura
    Research Service, James A Haley Veterans Hospital, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Biochemistry 43:15503-9. 2004
    ....
  29. pmc Increased insulin and leptin sensitivity in mice lacking acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, PO Box 419100, San Francisco, California 94141 9100, USA
    J Clin Invest 109:1049-55. 2002
    ..Our results suggest that inhibition of DGAT1 may be useful in treating insulin resistance and leptin resistance in human obesity...
  30. ncbi request reprint Cbl, IRS-1, and IRS-2 mediate effects of rosiglitazone on PI3K, PKC-lambda, and glucose transport in 3T3/L1 adipocytes
    Mary L Standaert
    Research Service, J A Haley Veterans Hospital, 13000 Bruce B Downs Boulevard, Tampa, Florida 33612, USA
    Endocrinology 143:1705-16. 2002
    ....
  31. ncbi request reprint Knockout of PKC alpha enhances insulin signaling through PI3K
    Michael Leitges
    Max Planck Institute for Experimental Endocrinology, 30625 Hannover, Germany
    Mol Endocrinol 16:847-58. 2002
    ....
  32. ncbi request reprint FoxO1 regulates multiple metabolic pathways in the liver: effects on gluconeogenic, glycolytic, and lipogenic gene expression
    Wenwei Zhang
    Departments of Medicine and Physiology and Biophysics, University of Illinois College of Medicine, Chicago, IL 60612, USA
    J Biol Chem 281:10105-17. 2006
    ....
  33. ncbi request reprint Repletion of atypical protein kinase C following RNA interference-mediated depletion restores insulin-stimulated glucose transport
    Mini P Sajan
    James A Haley Veterans Medical Center and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 281:17466-73. 2006
    ..These findings in knockdown and, more convincingly, rescue studies, strongly support the hypothesis that aPKCs are required for insulin-stimulated glucose transport in myocytes and adipocytes...
  34. pmc Sorbitol activates atypical protein kinase C and GLUT4 glucose transporter translocation/glucose transport through proline-rich tyrosine kinase-2, the extracellular signal-regulated kinase pathway and phospholipase D
    Mini P Sajan
    J A Haley Veterans Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, 13000 Bruce B Downs Blvd, Tampa, FL 33612, U S A
    Biochem J 362:665-74. 2002
    ..This mechanism contrasts with that of insulin, which primarily uses PI 3-kinase, D3-PO(4) polyphosphoinositides and PDK-1 to activate aPKCs...
  35. pmc Myostatin modulates adipogenesis to generate adipocytes with favorable metabolic effects
    Brian J Feldman
    Department of Pediatrics, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 103:15675-80. 2006
    ..In mice, these effects produced elevated systemic insulin sensitivity and resistance to diet-induced obesity. Modulation of the final stages of adipogenesis may provide a novel approach to understanding and treating metabolic disease...
  36. ncbi request reprint Cbl PYXXM motifs activate the P85 subunit of phosphatidylinositol 3-kinase, Crk, atypical protein kinase C, and glucose transport during thiazolidinedione action in 3T3/L1 and human adipocytes
    Atsushi Miura
    Research Service, James A Haley Veterans Administration Medical Center, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Biochemistry 42:14335-41. 2003
    ..We conclude that pYXXM motifs in Cbl serve to activate PI 3-kinase-dependent and possibly PI 3-kinase-independent pathways that are required for TZD-dependent glucose transport in adipocytes...
  37. ncbi request reprint Dissociation of hepatic steatosis and insulin resistance in mice overexpressing DGAT in the liver
    Mara Monetti
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell Metab 6:69-78. 2007
    ..Our results indicate that DGAT-mediated lipid accumulation in the liver is insufficient to cause insulin resistance and show that hepatic steatosis can occur independently of insulin resistance...
  38. pmc Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes
    Robert V Farese
    James A Haley Veterans Medical Center, Tampa, Florida 33612, USA
    J Clin Invest 117:2289-301. 2007
    ..These findings are particularly relevant because humans who have obesity, impaired glucose tolerance, and T2DM reportedly have defective activation and/or diminished levels of muscle aPKC...
  39. ncbi request reprint Increased lipid accumulation and insulin resistance in transgenic mice expressing DGAT2 in glycolytic (type II) muscle
    Malin C Levin
    Gladstone Institute of Cardiovascular Disease, 1650 Owens St, San Francisco, CA 94158, USA
    Am J Physiol Endocrinol Metab 293:E1772-81. 2007
    ..We conclude that DGAT2-mediated lipid deposition specifically in glycolytic muscle promotes insulin resistance in this tissue and may contribute to the development of diabetes...
  40. pmc Blocking VLDL secretion causes hepatic steatosis but does not affect peripheral lipid stores or insulin sensitivity in mice
    Kaori Minehira
    Gladstone Institute of Cardiovascular Disease, University of California San Francisco, CA 94158, USA
    J Lipid Res 49:2038-44. 2008
    ..Thus, blocking VLDL secretion causes hepatic steatosis without insulin resistance, and there is little effect on muscle triglyceride stores or adiposity...
  41. ncbi request reprint Role of adipocyte-derived factors in enhancing insulin signaling in skeletal muscle and white adipose tissue of mice lacking Acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    James A Haley Veterans Hospital, ACOS 151, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Diabetes 53:1445-51. 2004
    ..Our findings demonstrate that DGAT1 deficiency enhances insulin signaling in the skeletal muscle and WAT, in part through altered expression of adipocyte-derived factors that modulate insulin signaling in peripheral tissues...
  42. ncbi request reprint Insulin-induced activation of atypical protein kinase C, but not protein kinase B, is maintained in diabetic (ob/ob and Goto-Kakazaki) liver. Contrasting insulin signaling patterns in liver versus muscle define phenotypes of type 2 diabetic and high fat-i
    Mary L Standaert
    Research Service, James A Haley Veterans Medical Center and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 279:24929-34. 2004
    ....
  43. ncbi request reprint Activation of protein kinase C-zeta by insulin and phosphatidylinositol-3,4,5-(PO4)3 is defective in muscle in type 2 diabetes and impaired glucose tolerance: amelioration by rosiglitazone and exercise
    Mary Beeson
    Department of Internal Medicine, Division of Endocrinology and Metabolism, University of South Florida College of Medicine, Tampa, Florida, USA
    Diabetes 52:1926-34. 2003
    ..Defective aPKC activation contributes to skeletal muscle insulin resistance in IGT and type 2 diabetes, rosiglitazone improves insulin-stimulated aPKC activation, and exercise directly activates aPKCs in diabetic muscle...
  44. pmc Obesity resistance and enhanced glucose metabolism in mice transplanted with white adipose tissue lacking acyl CoA:diacylglycerol acyltransferase 1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Clin Invest 111:1715-22. 2003
    ..Our results highlight the importance of the endocrine function of WAT and suggest that an alteration in this function contributes to the increased energy expenditure and insulin sensitivity in DGAT1-deficient mice...
  45. ncbi request reprint Defective activation of atypical protein kinase C zeta and lambda by insulin and phosphatidylinositol-3,4,5-(PO4)(3) in skeletal muscle of rats following high-fat feeding and streptozotocin-induced diabetes
    Yoshinori Kanoh
    Research Service, James A Haley Veterans Administration Medical Center and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Endocrinology 144:947-54. 2003
    ....
  46. ncbi request reprint Essential role of protein kinase C zeta in the impairment of insulin-induced glucose transport in IRS-2-deficient brown adipocytes
    Monica Arribas
    Departamento de Bioquímica y Biología Molecular Instituto de Bioquímica, Centro Mixto CSIC UCM, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    FEBS Lett 536:161-6. 2003
    ..Our results support the essential role played by PKC zeta in the insulin resistance and impaired glucose uptake observed in IRS-2-deficient brown adipocytes...
  47. pmc Exercise improves phosphatidylinositol-3,4,5-trisphosphate responsiveness of atypical protein kinase C and interacts with insulin signalling to peptide elongation in human skeletal muscle
    Christian Frøsig
    Copenhagen Muscle Research Centre, Section of Human Physiology, Department of Exercise and Sport Sciences, University of Copenhagen, 13, Universitetsparken, DK 2100, Copenhagen, Denmark
    J Physiol 582:1289-301. 2007
    ..Furthermore, endurance exercise increased the responsiveness of aPKC to PIP3 providing a possible link to improved insulin-stimulated glucose uptake after exercise...
  48. ncbi request reprint Dissociation of obesity and impaired glucose disposal in mice overexpressing acyl coenzyme a:diacylglycerol acyltransferase 1 in white adipose tissue
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    Diabetes 51:3189-95. 2002
    ..Our findings support the lipotoxicity hypothesis that the deposition of triglycerides in insulin-sensitive tissues other than adipocytes causes insulin resistance...
  49. ncbi request reprint Analysis of energy expenditure at different ambient temperatures in mice lacking DGAT1
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94143, USA
    Am J Physiol Endocrinol Metab 284:E213-8. 2003
    ..Our findings offer insights into the mechanisms of hyperphagia and increased energy expenditure in a murine model of obesity resistance...
  50. ncbi request reprint Skeletal muscle insulin resistance in obesity-associated type 2 diabetes in monkeys is linked to a defect in insulin activation of protein kinase C-zeta/lambda/iota
    Mary L Standaert
    Research Service, James A Haley Veterans Administration Medical Center and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, USA
    Diabetes 51:2936-43. 2002
    ..Our findings suggest that defective activation of aPKCs contributes importantly to obesity-dependent development of skeletal muscle insulin resistance in prediabetic and type 2 diabetic monkeys...
  51. ncbi request reprint Protein kinase C-lambda knockout in embryonic stem cells and adipocytes impairs insulin-stimulated glucose transport
    Gautam Bandyopadhyay
    James A Haley Veterans Hospital and University of South Florida College of Medicine, Tampa, 33612, USA
    Mol Endocrinol 18:373-83. 2004
    ....
  52. ncbi request reprint Atrial natriuretic hormone prohormone gene expression in cardiac and extra-cardiac tissues of diabetic Goto-Kakizaki rats
    William R Gower
    Department of Biochemistry and Molecular Biology, James A Haley Veterans Hospital, Tampa, FL 33612, USA
    Mol Cell Biochem 252:263-71. 2003
    ..Diabetes is the first disease in which there is more upregulation of ANH prohormone in extracardiac tissues compared to upregulation within the heart itself...
  53. ncbi request reprint Increased atypical PKC activity in endurance-trained human skeletal muscle
    Jakob N Nielsen
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, DK 2100, Copenhagen, Denmark
    Biochem Biophys Res Commun 312:1147-53. 2003
    ..Protein expression of the kinases was similar in trained and sedentary muscle. The increased aPKC activity in exercise-trained subjects could be important in explaining the enhanced insulin action in these individuals...
  54. ncbi request reprint Activation of the ERK pathway and atypical protein kinase C isoforms in exercise- and aminoimidazole-4-carboxamide-1-beta-D-riboside (AICAR)-stimulated glucose transport
    Hubert C Chen
    James A Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 277:23554-62. 2002
    ..Our findings suggest that effects of exercise on glucose transport that are dependent on AMPK are mediated via PYK2, the ERK pathway, PLD, and aPKCs...
  55. pmc A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    J Lipid Res 46:2388-97. 2005
    ..We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin...
  56. ncbi request reprint DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis
    Kimberly K Buhman
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Biol Chem 277:25474-9. 2002
    ..Our findings indicate that multiple mechanisms for triacylglycerol synthesis in the intestine facilitate triacylglycerol absorption...
  57. pmc Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice
    Emily L Willner
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 100:1262-7. 2003
    ..Our results also support the rationale of pharmacological inhibition of ACAT2 as a therapy for atherosclerosis...
  58. ncbi request reprint Deficiency of acyl coenzyme a:diacylglycerol acyltransferase 1 increases leptin sensitivity in murine obesity models
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, University of California San Francisco, San Francisco, CA 94143, USA
    Endocrinology 143:2893-8. 2002
    ..Thus, the leptin-sensitizing effect of DGAT1 deficiency is present in both leptin-resistant and leptin-deficient genetic models of obesity and may occur in part by enhancing the effects of leptin in peripheral tissues...
  59. ncbi request reprint Fatty acids, triglycerides, and glucose metabolism: recent insights from knockout mice
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    Curr Opin Clin Nutr Metab Care 5:359-63. 2002
    ....
  60. ncbi request reprint Incorporation of deuterated RRR- or all-rac-alpha-tocopherol in plasma and tissues of alpha-tocopherol transfer protein--null mice
    Scott W Leonard
    Linus Pauling Institute, Oregon State University, Corvallis, USA
    Am J Clin Nutr 75:555-60. 2002
    ..In humans, 2R-alpha-tocopherol stereoisomers are preferentially maintained in the plasma, a function that has been attributed to hepatic alpha-tocopherol transfer protein (alpha-TTP), but this hypothesis has not been tested...
  61. ncbi request reprint Lipopenia and skin barrier abnormalities in DGAT2-deficient mice
    Scot J Stone
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 1900, USA
    J Biol Chem 279:11767-76. 2004
    ..DGAT1 was unable to compensate for the absence of DGAT2, supporting the hypothesis that the two enzymes play fundamentally different roles in mammalian triglyceride metabolism...
  62. pmc Leptin modulates the effects of acyl CoA:diacylglycerol acyltransferase deficiency on murine fur and sebaceous glands
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Clin Invest 109:175-81. 2002
    ..Our findings indicate that DGAT1 plays an important role in normal fur and sebaceous gland physiology and provide evidence that leptin modulates these processes in the skin...
  63. ncbi request reprint Plasma cholesteryl esters provided by lecithin:cholesterol acyltransferase and acyl-coenzyme a:cholesterol acyltransferase 2 have opposite atherosclerotic potential
    Richard G Lee
    Arteriosclerosis Research Program, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Circ Res 95:998-1004. 2004
    ..Overall, the data suggest that ACAT2-derived CE is the predominant atherogenic lipid in blood, and that an important goal for prevention of atherosclerosis is to limit ACAT2-derived CE accumulation in lipoproteins...
  64. ncbi request reprint Membrane topology and identification of key functional amino acid residues of murine acyl-CoA:diacylglycerol acyltransferase-2
    Scot J Stone
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    J Biol Chem 281:40273-82. 2006
    ..Our findings provide the first insights into the topography and molecular aspects of DGAT2 and related enzymes...
  65. ncbi request reprint Neuronal apoptosis linked to EglN3 prolyl hydroxylase and familial pheochromocytoma genes: developmental culling and cancer
    Sungwoo Lee
    Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Cell 8:155-67. 2005
    ..Moreover, EglN3 proapoptotic activity requires SDH activity because EglN3 is feedback inhibited by succinate. These studies suggest that failure of developmental apoptosis plays a role in pheochromocytoma pathogenesis...
  66. ncbi request reprint Determination of adipocyte size by computer image analysis
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, Cardiovascular Research Institute, University of California, San Francisco, CA 94141, USA
    J Lipid Res 43:986-9. 2002
    ..This method allows a large number of adipocytes to be measured rapidly with computer hardware and software that are readily available...
  67. pmc Effects of DGAT1 deficiency on energy and glucose metabolism are independent of adiponectin
    Ryan S Streeper
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Am J Physiol Endocrinol Metab 291:E388-94. 2006
    ..Our findings also suggest that the pharmacological inhibition of DGAT1 may be useful for treating human obesity and insulin resistance associated with low circulating adiponectin levels...
  68. ncbi request reprint Gene expression profile of oxidant stress and neurodegeneration in transgenic mice deficient in alpha-tocopherol transfer protein
    Kishorchandra Gohil
    Center for Comparative Respiratory and Medicine, Department of Internal Medicine, University of California, Davis, CA 95616, USA
    Free Radic Biol Med 35:1343-54. 2003
    ..The repression of specific genes that determine synaptic plasticity and neuronal development may account for suppressed electrophysiological activities of cortex and impaired behavior in Ttpa(-/-) mice...
  69. ncbi request reprint Fat breakdown: a function for CGI-58 (ABHD5) provides a new piece of the puzzle
    Chi Liang Eric Yen
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Cell Metab 3:305-7. 2006
    ....
  70. ncbi request reprint Short-term overexpression of DGAT1 or DGAT2 increases hepatic triglyceride but not VLDL triglyceride or apoB production
    John S Millar
    Department of Pharmacology, University of Pennsylvania, Philadelphia, PA, USA
    J Lipid Res 47:2297-305. 2006
    ..In the presence of adequate cytoplasmic lipid stores, factors other than triglyceride synthesis are rate-limiting for VLDL production...
  71. ncbi request reprint Inhibition of triglyceride synthesis as a treatment strategy for obesity: lessons from DGAT1-deficient mice
    Hubert C Chen
    Department of Medical Sciences, Amgen Inc, Thousand Oaks, USA
    Arterioscler Thromb Vasc Biol 25:482-6. 2005
    ..These findings suggest that pharmacological inhibition of DGAT1 may be a feasible therapeutic strategy for human obesity and type 2 diabetes...
  72. ncbi request reprint ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: impact on hepatic cholesterol homeostasis
    Joyce J Repa
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8887, USA
    Hepatology 40:1088-97. 2004
    ..Loss of ACAT2 activity may result in unesterified cholesterol being absorbed via an ABCA1-mediated basolateral efflux pathway...
  73. ncbi request reprint LPA3 receptor mediates chemotaxis of immature murine dendritic cells to unsaturated lysophosphatidic acid (LPA)
    Liana C Chan
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    J Leukoc Biol 82:1193-200. 2007
    ..In summary, our results indicate that immature mouse DCs migrate preferentially in response to unsaturated LPA and that LPA(3) is important in this response...