JOSEPH FALKE

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. pmc Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study
    Huai Chun Chen
    Department of Chemistry and Biochemistry and the Molecular Biophysics Program, University of Colorado, Boulder, Colorado, United States of America
    PLoS ONE 7:e33640. 2012
  2. pmc Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex
    J A Bornhorst
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309-0215, USA
    J Gen Physiol 118:693-710. 2001
  3. pmc Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309 0215, USA
    Curr Opin Struct Biol 10:462-9. 2000
  4. pmc Cooperativity between bacterial chemotaxis receptors
    Joseph J Falke
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 99:6530-2. 2002
  5. pmc The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309 0215, USA
    Annu Rev Cell Dev Biol 13:457-512. 1997
  6. pmc Enzymology. A moving story
    Joseph J Falke
    Molecular Biophysics Program and The Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Science 295:1480-1. 2002
  7. pmc Transmembrane signaling in bacterial chemoreceptors
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, 80309 0215, Boulder, CO, USA
    Trends Biochem Sci 26:257-65. 2001
  8. pmc Quantitative analysis of aspartate receptor signaling complex reveals that the homogeneous two-state model is inadequate: development of a heterogeneous two-state model
    Joshua A Bornhorst
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    J Mol Biol 326:1597-614. 2003
  9. pmc Adaptation mechanism of the aspartate receptor: electrostatics of the adaptation subdomain play a key role in modulating kinase activity
    Diane J Starrett
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 44:1550-60. 2005
  10. pmc Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching
    Matthew D Coleman
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 44:7687-95. 2005

Collaborators

Detail Information

Publications40

  1. pmc Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study
    Huai Chun Chen
    Department of Chemistry and Biochemistry and the Molecular Biophysics Program, University of Colorado, Boulder, Colorado, United States of America
    PLoS ONE 7:e33640. 2012
    ....
  2. pmc Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex
    J A Bornhorst
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309-0215, USA
    J Gen Physiol 118:693-710. 2001
    ..It follows that the attractant and adaptation signals drive the same conformational change between the two settings of a toggle. An approach that quantifies the fractional occupancy of the on- and off-states is illustrated...
  3. pmc Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309 0215, USA
    Curr Opin Struct Biol 10:462-9. 2000
    ..These results represent an important step toward a mechanistic understanding of receptor-to-kinase information transfer...
  4. pmc Cooperativity between bacterial chemotaxis receptors
    Joseph J Falke
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 99:6530-2. 2002
  5. pmc The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309 0215, USA
    Annu Rev Cell Dev Biol 13:457-512. 1997
    ..Overall, the chemosensory pathway and the propulsion system it regulates provide an ideal system in which to probe molecular principles underlying complex cellular signaling and behavior...
  6. pmc Enzymology. A moving story
    Joseph J Falke
    Molecular Biophysics Program and The Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Science 295:1480-1. 2002
  7. pmc Transmembrane signaling in bacterial chemoreceptors
    J J Falke
    Department of Chemistry and Biochemistry, University of Colorado, 80309 0215, Boulder, CO, USA
    Trends Biochem Sci 26:257-65. 2001
    ..Multiple, independent lines of evidence indicate that, in the periplasmic and transmembrane domains, conformational signaling is a piston-type sliding of the signaling helix towards the cytoplasm...
  8. pmc Quantitative analysis of aspartate receptor signaling complex reveals that the homogeneous two-state model is inadequate: development of a heterogeneous two-state model
    Joshua A Bornhorst
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    J Mol Biol 326:1597-614. 2003
    ..This method suggests that the ratio of receptor dimers to CheA dimers in the assembled complex is 6:1 or less...
  9. pmc Adaptation mechanism of the aspartate receptor: electrostatics of the adaptation subdomain play a key role in modulating kinase activity
    Diane J Starrett
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 44:1550-60. 2005
    ..Overall, this study highlights the importance of electrostatics in signal transduction and regulation of kinase activity by the cytoplasmic domain of the aspartate receptor...
  10. pmc Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching
    Matthew D Coleman
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 44:7687-95. 2005
    ....
  11. pmc Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase control module of the aspartate receptor
    Kalin E Swain
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 48:9266-77. 2009
    ....
  12. pmc C2 domain of protein kinase C alpha: elucidation of the membrane docking surface by site-directed fluorescence and spin labeling
    Susy C Kohout
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 42:1254-65. 2003
    ..The data support a model in which the beta-strands are tilted toward the parallel orientation relative to the membrane surface...
  13. pmc Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor
    Aaron S Miller
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 43:1763-70. 2004
    ....
  14. pmc CheA Kinase of bacterial chemotaxis: chemical mapping of four essential docking sites
    Aaron S Miller
    Molecular Biophysics Program, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 45:8699-711. 2006
    ..Finally, the results provide new structural constraints allowing the development of improved models for core complex architecture...
  15. pmc Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: a disulfide mapping study
    Kalin E Swain
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 46:13684-95. 2007
    ..A model incorporating HAMP into the full receptor structure is proposed...
  16. pmc Mechanism of specific membrane targeting by C2 domains: localized pools of target lipids enhance Ca2+ affinity
    John A Corbin
    Molecular Biophysics Program, and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 46:4322-36. 2007
    ....
  17. pmc C2 domains of protein kinase C isoforms alpha, beta, and gamma: activation parameters and calcium stoichiometries of the membrane-bound state
    Susy C Kohout
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 41:11411-24. 2002
    ....
  18. pmc Ca2+ activation of the cPLA2 C2 domain: ordered binding of two Ca2+ ions with positive cooperativity
    Nathan J Malmberg
    Molecular Biophysics Program, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80301 0215, USA
    Biochemistry 43:16320-8. 2004
    ..This initial binding event prepares the conformation and protonation state of the remaining site for Ca(2+) binding, enabling the second Ca(2+) ion to bind with higher affinity than the first as required for positive cooperativity...
  19. pmc Use of EPR power saturation to analyze the membrane-docking geometries of peripheral proteins: applications to C2 domains
    Nathan J Malmberg
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Annu Rev Biophys Biomol Struct 34:71-90. 2005
    ..Representative applications to Ca(2+)-activated, membrane-docking C2 domains are described...
  20. pmc Effect of PIP2 binding on the membrane docking geometry of PKC alpha C2 domain: an EPR site-directed spin-labeling and relaxation study
    Kyle E Landgraf
    Department of Chemistry and Biochemistry and the Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 47:8301-16. 2008
    ..Finally, the results demonstrate that EPR relaxation methods are sufficiently sensitive to detect signaling-induced changes in the membrane docking geometries of peripheral membrane proteins...
  21. pmc Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation
    Ryan S Mehan
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 42:2952-9. 2003
    ....
  22. pmc Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain
    John H Evans
    Molecular Biophysics Program and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Boulder, CO 80309 0215, USA
    Mol Biol Cell 17:56-66. 2006
    ..Instead, targeting specificity is provided by basic residues on beta-strands 3-4, which bind to plasma membrane PIP2...
  23. pmc Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: cysteine and disulfide scanning studies
    Susanna E Winston
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309 0215, USA
    Biochemistry 44:12655-66. 2005
    ..A mechanistic model is proposed in which a signal is transmitted through the adaptation subdomain by a change in supercoiling of the four-helix bundle...
  24. ncbi request reprint Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains
    Eric A Nalefski
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA
    Methods Mol Biol 172:295-303. 2002
  25. pmc Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling
    Nathan J Malmberg
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 42:13227-40. 2003
    ....
  26. pmc Single-molecule fluorescence studies of a PH domain: new insights into the membrane docking reaction
    Jefferson D Knight
    Molecular Biophysics Program, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, USA
    Biophys J 96:566-82. 2009
    ..Overall, this study shows that in vitro single-molecule TIRFM provides a new window into the molecular mechanisms of membrane docking reactions...
  27. pmc Molecular mechanism of an oncogenic mutation that alters membrane targeting: Glu17Lys modifies the PIP lipid specificity of the AKT1 PH domain
    Kyle E Landgraf
    Department of Chemistry and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 47:12260-9. 2008
    ..Moreover, the findings strongly implicate the native Glu17 side chain as a key element of PIP lipid specificity in the wild-type AKT1 PH domain. Other PH domains may employ an analogous anionic residue to control PIP specificity...
  28. pmc The core signaling proteins of bacterial chemotaxis assemble to form an ultrastable complex
    Annette H Erbse
    Department of Chemistry, and Biochemistry and Molecular Biophysics Program, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 48:6975-87. 2009
    ..Possible biological functions of ultrastability are discussed...
  29. pmc GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid
    John A Corbin
    Molecular Biophysics Program and The Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 43:16161-73. 2004
    ....
  30. pmc Thermal domain motions of CheA kinase in solution: Disulfide trapping reveals the motional constraints leading to trans-autophosphorylation
    Susan L Gloor
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, 80309 0215, USA
    Biochemistry 48:3631-44. 2009
    ..Finally, a working model is proposed for the motional constraints that limit the P1 domain to the region of space near the P4' catalytic domain of the sister subunit...
  31. pmc Ca2+ influx is an essential component of the positive-feedback loop that maintains leading-edge structure and activity in macrophages
    John H Evans
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Proc Natl Acad Sci U S A 104:16176-81. 2007
    ..These findings support the working hypothesis that a local, leading-edge Ca(2+) signal recruits PKCalpha as a central player in the positive-feedback loop...
  32. pmc Cation charge and size selectivity of the C2 domain of cytosolic phospholipase A(2)
    Eric A Nalefski
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309 0215, USA
    Biochemistry 41:1109-22. 2002
    ..These features are proposed to stem from the unique structural features of the metal ion-binding site in the C2 domain...
  33. ncbi request reprint Chemotaxis receptors and signaling
    Aaron F Miller
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Adv Protein Chem 68:393-444. 2004
    ..Extensive mutagenesis studies have been carried out, and have begun to identify critical residues involved in ligand binding, receptor activation, and transmembrane signaling...
  34. pmc Self-induced docking site of a deeply embedded peripheral membrane protein
    Simon Jaud
    Department of Chemistry, and Department of Physiology and Biophysics, University of California, Irvine, California, USA
    Biophys J 92:517-24. 2007
    ....
  35. pmc The PICM chemical scanning method for identifying domain-domain and protein-protein interfaces: applications to the core signaling complex of E. coli chemotaxis
    Randal B Bass
    Analytical Sciences, Amgen Inc, Seattle, WA, USA
    Methods Enzymol 423:3-24. 2007
    ....
  36. pmc Bacterial chemoreceptors: high-performance signaling in networked arrays
    Gerald L Hazelbauer
    Department of Biochemistry, University of Missouri Columbia, Columbia, MO 65211, USA
    Trends Biochem Sci 33:9-19. 2008
    ..These new data suggest multiple levels of molecular interactions, each of which contribute specific functional features and together create a sophisticated signaling device...
  37. ncbi request reprint Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling
    April A Frazier
    Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, Virginia 22904 4319, USA
    Biochemistry 41:6282-92. 2002
    ..Upon membrane docking, spin labels in the Ca(2+)-binding loops exhibit decreases in local motion, suggesting either changes in tertiary contacts due to protein conformational changes and/or interactions with lipid...
  38. pmc Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis
    Randal B Bass
    Analytical Sciences, Amgen Inc, Seattle, WA, USA
    Methods Enzymol 423:25-51. 2007
    ..This chapter details these methods and illustrates applications to two proteins from the bacterial chemotaxis pathway: the periplasmic galactose binding protein and the transmembrane aspartate receptor...
  39. pmc Chemotaxis receptor complexes: from signaling to assembly
    Robert G Endres
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Comput Biol 3:e150. 2007
    ..We propose that the kinetics of complex assembly can be measured in vitro from the temporal response to a perturbation of the complex free energies, e.g., by addition of ligand...

Research Grants39

  1. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2003
    ..Overall, the broad goal of these studies is to understand the mechanisms of transmembrane signaling, receptor adaptation, and kinase regulation in a fully assembled, multi-protein signaling complex. ..
  2. Membrane Proteins - Structure and Mechanism
    JOSEPH FALKE; Fiscal Year: 2002
    ..Overall, the conference will provide a forum in which membrane protein researchers come together to present exciting new results with broad, significant implications for membrane protein structure and mechanism. ..
  3. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2002
    ..Overall, the broad goal of these studies is to understand the mechanisms of transmembrane signaling, receptor adaptation, and kinase regulation in a fully assembled, multi-protein signaling complex. ..
  4. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2001
    ..Overall, the broad goal of these studies is to understand the mechanisms of transmembrane signaling, receptor adaptation, and kinase regulation in a fully assembled, multi-protein signaling complex. ..
  5. ACTIVATION & DYNAMICS OF RECEPTORS & SIGNALING PROTEINS
    JOSEPH FALKE; Fiscal Year: 2000
    ..19F NMR and other solution methods will probe the effects of phosphorylation on both the conformation and dynamics of the activation loop that directly controls kinase activity. ..
  6. ACTIVATION & DYNAMICS OF RECEPTORS & SIGNALING PROTEINS
    JOSEPH FALKE; Fiscal Year: 1999
    ..19F NMR and other solution methods will probe the effects of phosphorylation on both the conformation and dynamics of the activation loop that directly controls kinase activity. ..
  7. BIOPHYSICAL STUDIES OF PROTEIN STRUCTURE AND DYNAMICS
    JOSEPH FALKE; Fiscal Year: 1992
    ..In addition, bacterial binding proteins have been useful as detectors in assays for genetic diseases and treatment (reviewed in ref. 39)...
  8. BIOPHYSICAL STUDIES OF PROTEIN STRUCTURE AND DYNAMICS
    JOSEPH FALKE; Fiscal Year: 1991
    ..In addition, bacterial binding proteins have been useful as detectors in assays for genetic diseases and treatment (reviewed in ref. 39)...
  9. Mechanisms of Membrane Targeting by C2 and PH Domains
    JOSEPH FALKE; Fiscal Year: 2006
    ..Overall, the results of the proposed studies will have significant implications for a molecular understanding of C2 and PH domain signaling in human health and disease. ..
  10. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2005
    ..Overall, the broad goal of these studies is to understand the mechanisms of transmembrane signaling, receptor adaptation, and kinase regulation in a fully assembled, multi-protein signaling complex. ..
  11. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2006
    ..Overall, the broad goal of these studies is to understand the mechanisms of receptor transmembrane signaling, receptor adaptation, and kinase regulation in the native environment that includes the membrane and other pathway components. ..
  12. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2007
    ..Overall, the broad goal of these studies is to understand the mechanisms of receptor transmembrane signaling, receptor adaptation, and kinase regulation in the native environment that includes the membrane and other pathway components. ..
  13. Predoctoral Training Molecular Biophysics
    JOSEPH FALKE; Fiscal Year: 2007
    ....
  14. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2009
    ..abstract_text> ..
  15. Calcium Activated Membrane Targeting by the C2 Domain
    JOSEPH FALKE; Fiscal Year: 2003
    ..Furthermore, comparative studies of C2 domains will provide information crucial to genomic analyses of many signaling pathways. ..
  16. RECEPTORS & SIGNALING PROTEINS--DYNAMICS & ACTIVATION
    JOSEPH FALKE; Fiscal Year: 1993
    ..In addition, the E. coli components exhibit interesting parallels with certain eukaryotic receptors and signaling proteins, such as the insulin receptor and H-ras, respectively...
  17. Calcium Activated Membrane Targeting by the C2 Domain
    JOSEPH FALKE; Fiscal Year: 2001
    ..Furthermore, comparative studies of C2 domains will provide information crucial to genomic analyses of many signaling pathways. ..
  18. Calcium Activated Membrane Targeting by the C2 Domain
    JOSEPH FALKE; Fiscal Year: 2002
    ..Furthermore, comparative studies of C2 domains will provide information crucial to genomic analyses of many signaling pathways. ..
  19. BIOPHYSICAL STUDIES OF PROTEIN STRUCTURE AND DYNAMICS
    JOSEPH FALKE; Fiscal Year: 1990
    ..In addition, bacterial binding proteins have been useful as detectors in assays for genetic diseases and treatment (reviewed in ref. 39)...
  20. Mechanisms of Regulated Membrane Targeting
    JOSEPH FALKE; Fiscal Year: 2009
    ..Preliminary results reveal that the E17K mutation enhances membrane targeting by changing the target lipid specificity of the PH domain, thereby defining the molecular mechanism of this important oncogenic mutation. ..
  21. Mechanisms of Regulated Membrane Targeting
    Joseph J Falke; Fiscal Year: 2010
    ..Preliminary results reveal that the E17K mutation enhances membrane targeting by changing the target lipid specificity of the PH domain, thereby defining the molecular mechanism of this important oncogenic mutation. ..
  22. Calcium Activated Membrane Targeting by the C2 Domain
    JOSEPH FALKE; Fiscal Year: 2001
    ..Furthermore, comparative studies of C2 domains will provide information crucial to genomic analyses of many signaling pathways. ..
  23. Mechanisms of Membrane Targeting by C2 and PH Domains
    JOSEPH FALKE; Fiscal Year: 2007
    ..Overall, the results of the proposed studies will have significant implications for a molecular understanding of C2 and PH domain signaling in human health and disease. ..
  24. Activation and Dynamics of Receptors and Kinases
    JOSEPH FALKE; Fiscal Year: 2004
    ..Overall, the broad goal of these studies is to understand the mechanisms of transmembrane signaling, receptor adaptation, and kinase regulation in a fully assembled, multi-protein signaling complex. ..
  25. Calcium Activated Membrane Targeting by the C2 Domain
    JOSEPH FALKE; Fiscal Year: 2004
    ..Furthermore, comparative studies of C2 domains will provide information crucial to genomic analyses of many signaling pathways. ..
  26. Activation and Dynamics of Receptors and Kinases
    Joseph J Falke; Fiscal Year: 2010
    ..Moreover, it is the first signaling circuit found to be ultrastable, providing a platform for understanding ultrastability and for designing new ultrastable biosensors. ..
  27. Mechanisms of Membrane Targeting by C2 and PH Domains
    JOSEPH FALKE; Fiscal Year: 2005
    ..Overall, the results of the proposed studies will have significant implications for a molecular understanding of C2 and PH domain signaling in human health and disease. ..