J N Fain

Summary

Affiliation: University of Tennessee
Country: USA

Publications

  1. ncbi Stimulation of lipolysis but not of leptin release by growth hormone is abolished in adipose tissue from Stat5a and b knockout mice
    J N Fain
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis, Tennessee 38163, USA
    Biochem Biophys Res Commun 263:201-5. 1999
  2. ncbi Most of the interleukin 1 receptor antagonist, cathepsin S, macrophage migration inhibitory factor, nerve growth factor, and interleukin 18 release by explants of human adipose tissue is by the non-fat cells, not by the adipocytes
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 55:1113-21. 2006
  3. ncbi Haptoglobin release by human adipose tissue in primary culture
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Lipid Res 45:536-42. 2004
  4. ncbi Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Endocrinology 145:2273-82. 2004
  5. ncbi TNFalpha release by the nonfat cells of human adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Int J Obes Relat Metab Disord 28:616-22. 2004
  6. ncbi Insulin enhances vascular endothelial growth factor, interleukin-8, and plasminogen activator inhibitor 1 but not interleukin-6 release by human adipocytes
    John N Fain
    Department of Molecular Science, University of Tennessee Health Science Center, Memphis, TN 38163 3409, USA
    Metabolism 54:220-6. 2005
  7. ncbi Involvement of multiple signaling pathways in the post-bariatric induction of IL-6 and IL-8 mRNA and release in human visceral adipose tissue
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Biochem Pharmacol 69:1315-24. 2005
  8. ncbi Regulation of monocyte chemoattractant protein 1 (MCP-1) release by explants of human visceral adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Int J Obes (Lond) 29:1299-307. 2005
  9. ncbi Regulation of adiponectin release and demonstration of adiponectin mRNA as well as release by the non-fat cells of human omental adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 3816, USA
    Int J Obes (Lond) 32:429-35. 2008
  10. ncbi Release in vitro of adipsin, vascular cell adhesion molecule 1, angiotensin 1-converting enzyme, and soluble tumor necrosis factor receptor 2 by human omental adipose tissue as well as by the nonfat cells and adipocytes
    John N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 56:1583-90. 2007

Detail Information

Publications39

  1. ncbi Stimulation of lipolysis but not of leptin release by growth hormone is abolished in adipose tissue from Stat5a and b knockout mice
    J N Fain
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis, Tennessee 38163, USA
    Biochem Biophys Res Commun 263:201-5. 1999
    ..These results suggest that the lipolytic action of GH involves the Stat5 proteins while the insulin-like effects of GH on glucose metabolism and leptin release involve different mechanisms...
  2. ncbi Most of the interleukin 1 receptor antagonist, cathepsin S, macrophage migration inhibitory factor, nerve growth factor, and interleukin 18 release by explants of human adipose tissue is by the non-fat cells, not by the adipocytes
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 55:1113-21. 2006
    ..Our results suggest that the non-fat cells in human adipose tissue contribute to most of the release of NGF, IL-18, IL-1Ra, cathepsin S, and MIF seen during primary culture of adipose tissue explants from obese women...
  3. ncbi Haptoglobin release by human adipose tissue in primary culture
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Lipid Res 45:536-42. 2004
    ..However, the amount of haptoglobin released by human adipose tissue explants in primary culture was quite low in relationship to the circulating level of haptoglobin...
  4. ncbi Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Endocrinology 145:2273-82. 2004
    ..Furthermore, most of the adipokine release by the nonfat cells of adipose tissue was due to cells retained in the tissue matrix after collagenase digestion...
  5. ncbi TNFalpha release by the nonfat cells of human adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Int J Obes Relat Metab Disord 28:616-22. 2004
    ..The second aim was to examine the correlation between body mass index (BMI) and the subsequent release of adiponectin and TNFalpha by explants of human subcutaneous and visceral adipose tissue incubated in primary culture for 48 h...
  6. ncbi Insulin enhances vascular endothelial growth factor, interleukin-8, and plasminogen activator inhibitor 1 but not interleukin-6 release by human adipocytes
    John N Fain
    Department of Molecular Science, University of Tennessee Health Science Center, Memphis, TN 38163 3409, USA
    Metabolism 54:220-6. 2005
    ..The ability of insulin to stimulate VEGF formation by adipocytes suggests that the elevated circulating levels of insulin in obesity promote angiogenesis in adipose tissue as well as the enhanced accumulation of fat in human adipocytes...
  7. ncbi Involvement of multiple signaling pathways in the post-bariatric induction of IL-6 and IL-8 mRNA and release in human visceral adipose tissue
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Biochem Pharmacol 69:1315-24. 2005
    ..These cytokines, and probably other factors as well, enhance IL-6 and IL-8 mRNA accumulation in human adipose tissue explants through mechanisms involving the p38 MAPK and NFkappaB pathways...
  8. ncbi Regulation of monocyte chemoattractant protein 1 (MCP-1) release by explants of human visceral adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Int J Obes (Lond) 29:1299-307. 2005
    ..Monocyte chemoattractant protein-1 (MCP-1) is a chemokine involved in monocyte recruitment during inflammation whose plasma level is elevated in obesity...
  9. ncbi Regulation of adiponectin release and demonstration of adiponectin mRNA as well as release by the non-fat cells of human omental adipose tissue
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 3816, USA
    Int J Obes (Lond) 32:429-35. 2008
    ....
  10. ncbi Release in vitro of adipsin, vascular cell adhesion molecule 1, angiotensin 1-converting enzyme, and soluble tumor necrosis factor receptor 2 by human omental adipose tissue as well as by the nonfat cells and adipocytes
    John N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 56:1583-90. 2007
    ..Furthermore, the enhanced release of VCAM-1 and ACE seen in omental adipose tissue explants as compared with explants of subcutaneous adipose tissue is due to release by nonfat cells...
  11. doi Identification of omentin mRNA in human epicardial adipose tissue: comparison to omentin in subcutaneous, internal mammary artery periadventitial and visceral abdominal depots
    J N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Int J Obes (Lond) 32:810-5. 2008
    ....
  12. doi Comparison of messenger RNA distribution for 60 proteins in fat cells vs the nonfat cells of human omental adipose tissue
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 57:1005-15. 2008
    ..We conclude that the mRNAs for inflammatory proteins are primarily present in the nonfat cells of human omental adipose tissue...
  13. ncbi Transforming growth factor beta1 release by human adipose tissue is enhanced in obesity
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 54:1546-51. 2005
    ..The release of TGF-beta1 was primarily by the nonfat cells of human adipose tissue because release by adipocytes was less than 10% of that by the nonfat cells of adipose tissue...
  14. ncbi Inhibition of leptin release by atrial natriuretic peptide (ANP) in human adipocytes
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Biochem Pharmacol 65:1883-8. 2003
    ..Our data demonstrate that the lipolytic effect of ANP is probably secondary to stimulation of cyclic GMP accumulation in human adipocytes, and this is accompanied by an inhibition of leptin release...
  15. ncbi Regulation of leptin release and lipolysis by PGE2 in rat adipose tissue
    J N Fain
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis 38163, USA
    Prostaglandins Other Lipid Mediat 62:343-50. 2000
    ..However, while the endogenous formation of PGE2 does not appear to regulate basal lipolysis or leptin release, it may play a role in the activation of lipolysis by catecholamines...
  16. doi Release of 12 adipokines by adipose tissue, nonfat cells, and fat cells from obese women
    John N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA
    Obesity (Silver Spring) 18:890-6. 2010
    ..In contrast that of ICAM-1, FABP-4, GPX-3, visfatin/PBEF/Nampt, CD14, lipocalin-2, LP, and endothelin-1 was significantly greater in tissue from women with a total fat mass of 65 kg...
  17. ncbi Abnormalities in the functioning of adipocytes from R6/2 mice that are transgenic for the Huntington's disease mutation
    J N Fain
    Departments of Molecular Sciences and Anatomy and Neurobiology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Hum Mol Genet 10:145-52. 2001
    ..These data suggest that the obesity observed at 8-9 weeks in R6/2 mice may stem from a defect in fat breakdown by adipocytes...
  18. ncbi Obesity is induced in mice heterozygous for cyclooxygenase-2
    J N Fain
    Department of Molecular Sciences, College of Medicine, The University of Tennessee Health Science Center, Memphis 38163, USA
    Prostaglandins Other Lipid Mediat 65:199-209. 2001
    ..These data indicate that heterozygous COX-2 mice develop obesity which is not secondary to a defect in leptin release by adipose tissue...
  19. ncbi Comparison of PGE2, prostacyclin and leptin release by human adipocytes versus explants of adipose tissue in primary culture
    J N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN, USA
    Prostaglandins Leukot Essent Fatty Acids 67:467-73. 2002
    ..In conclusion, these data indicate that leptin release is exclusively a function of adipocytes while prostanoids are made by both adipocytes and the other cells present in human adipose tissue..
  20. ncbi Resistin release by human adipose tissue explants in primary culture
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Biochem Biophys Res Commun 300:674-8. 2003
    ..The present data suggest that resistin release by explants of human adipose tissue in primary culture is largely derived from the non-fat cells present in the explants...
  21. ncbi Stimulation of leptin release by arachidonic acid and prostaglandin E(2) in adipose tissue from obese humans
    J N Fain
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis, TN 38163, USA
    Metabolism 50:921-8. 2001
    ..These data suggest that when upregulation of COX-2 is blocked by dexamethasone, exogenous PGE(2) enhances both leptin release and leptin mRNA accumulation by explants of human adipose tissue in primary culture...
  22. ncbi Regulation of leptin release by insulin, glucocorticoids, G(i)-coupled receptor agonists, and pertussis toxin in adipocytes and adipose tissue explants from obese humans in primary culture
    Alie Kanu
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38136, USA
    Metabolism 52:60-6. 2003
    ....
  23. ncbi Regulation of oxytocin-induced phosphoinositide breakdown in adipocytes by adenosine, isoproterenol and insulin
    H M Lee
    Department of Biochemistry, University of Tennessee, Memphis 38163
    Biochim Biophys Acta 1013:73-9. 1989
    ..These results indicate that, in adipocytes, phosphoinositide breakdown stimulated by oxytocin is enhanced by adenosine, inhibited by isoproterenol and, under some conditions is inhibited by insulin...
  24. ncbi Stimulation of leptin release by actinomycin D in rat adipocytes
    J N Fain
    Department of Biochemistry, College of Medicine, University of Tennessee at Memphis, 38163, USA
    Biochem Pharmacol 55:1309-14. 1998
    ..These results point to a unique regulation of leptin release and leptin mRNA levels by actinomycin D...
  25. ncbi Laparoscopic bariatric patients' will to help: the foundation of research
    David S Tichansky
    Section of Minimally Invasive Surgery, Department of Surgery, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Surg Obes Relat Dis 3:180-3. 2007
    ..Bariatric patient enthusiasm for research participation has never been objectively measured. Our hypothesis was that most laparoscopic bariatric surgery patients would participate in clinical research...
  26. doi Stimulation of human omental adipose tissue lipolysis by growth hormone plus dexamethasone
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Mol Cell Endocrinol 295:101-5. 2008
    ..We conclude that the direct lipolytic effect of GH is accompanied by an increase in HSL mRNA in the presence of DEX, but GH also increased the mRNAs for other proteins that could explain all or part of its lipolytic action...
  27. ncbi Site -2548 of the leptin gene is associated with gender-specific trends in newborn size and cord leptin levels
    Ronald M Adkins
    Children s Foundation Research Center and Center of Genomics and Bioinformatics, University of Tennessee Health Science Center, Memphis, TN 38122, USA
    Int J Pediatr Obes 2:130-7. 2007
    ..We examined the relationships among leptin gene polymorphisms, size for gestational age, umbilical cord leptin, and gender...
  28. ncbi Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels
    Ronald M Adkins
    Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee 38103, USA
    Pediatr Res 62:451-5. 2007
    ..Analyses of haplotypes inferred from these two SNPs demonstrate a significant relationship between paternally transmitted haplotypes and newborn IGF-II levels, but no association with maternally transmitted haplotypes...
  29. ncbi Are African-Americans as successful as Caucasians after laparoscopic gastric bypass?
    Atul K Madan
    Section of Minimally Invasive Surgery, Department of Surgery, University of Tennessee Health Science Center, 956 Court Ave, Room G210, Memphis, TN 38163, USA
    Obes Surg 17:460-4. 2007
    ..Our hypothesis was that AAs are just as successful as CA after LRYGBP in terms of weight loss and comorbidity improvement...
  30. doi Dexamethasone and the inflammatory response in explants of human omental adipose tissue
    John N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Mol Cell Endocrinol 315:292-8. 2010
    ..We conclude that dexamethasone is a selective anti-inflammatory agent since it inhibits only about one-fourth of the proteins up-regulated during in vitro incubation of human omental adipose tissue...
  31. ncbi Comparison of IL-8, IL-6 and PGE(2) formation by visceral (omental) adipose tissue of obese Caucasian compared to African-American women
    Atul K Madan
    Department of Surgery, College of Medicine University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Obes Surg 16:1342-50. 2006
    ..We hypothesized that there are ethnic differences in inflammatory markers and medical co-morbidities...
  32. ncbi Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cells
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Vitam Horm 74:443-77. 2006
    ..In conclusion, there is an increasing recognition that adipose tissue is an endocrine organ that secretes leptin and adiponectin along with a host of other paracrine and endocrine factors in addition to free fatty acids...
  33. doi Human epicardial adipokine messenger RNAs: comparisons of their expression in substernal, subcutaneous, and omental fat
    John N Fain
    Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Metabolism 59:1379-86. 2010
    ..The data do not support the hypothesis that most of the inflammatory adipokines are expressed at high levels in epicardial fat of humans...
  34. pmc Release of inflammatory mediators by human adipose tissue is enhanced in obesity and primarily by the nonfat cells: a review
    John N Fain
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Mediators Inflamm 2010:513948. 2010
    ..The conclusion is that the release of many inflammatory adipokines by adipose tissue is enhanced in obese humans...
  35. ncbi Altered phosphoinositide-specific phospholipase C and adenylyl cyclase in brain cortical membranes of cats with GM1 and GM2 gangliosidosis
    E Claro
    Department of Biochemistry, University of Tennessee, Memphis 38163
    Brain Res Mol Brain Res 11:265-71. 1991
    ..These data suggest that the activation of phosphoinositide-specific phospholipase C in brain membranes by guanine nucleotide binding proteins is markedly impaired in GM1 and GM2 gangliosidoses...
  36. ncbi Human epicardial adipose tissue: a review
    Harold S Sacks
    Division of Endocrinology and Metabolism, University of Tennessee, and Baptist Hospital Heart Institute, Memphis, TN, USA
    Am Heart J 153:907-17. 2007
    ..Further studies should be done to test the hypothesis that adipokines produced by and released from human epicardial adipose tissue might contribute locally to the pathogenesis of coronary atherosclerosis...
  37. doi Uncoupling protein-1 and related messenger ribonucleic acids in human epicardial and other adipose tissues: epicardial fat functioning as brown fat
    Harold S Sacks
    Department of Medicine, College of Medicine, University of Tennessee Health Science Center Memphis, Tennessee 38163, USA
    J Clin Endocrinol Metab 94:3611-5. 2009
    ..Uncoupling protein-1 (UCP-1) is the inner mitochondrial membrane protein that is a specific marker for and mediator of nonshivering thermogenesis in brown adipocytes...
  38. ncbi Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissue
    Jens M Bruun
    Department of Endocrinology and Metabolism C, Aarhus Amtssygehus, Aarhus University Hospital and Faculty of Health Sciences, Aarhus University, Tage Hansensgade 2, DK 8000 Aarhus C, Denmark
    Am J Physiol Endocrinol Metab 286:E8-13. 2004
    ..The high levels of IL-8 release from human adipose tissue and accumulation of this tissue in obese subjects may account for some of the increase in circulating IL-8 observed in obesity...