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Genomes and Genes | J N FainSummaryAffiliation: University of Tennessee Country: USA Publications
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Stimulation of lipolysis but not of leptin release by growth hormone is abolished in adipose tissue from Stat5a and b knockout miceJ N Fain
Department of Biochemistry, College of Medicine, University of Tennessee, Memphis, Tennessee 38163, USA
Biochem Biophys Res Commun 263:201-5. 1999..These results suggest that the lipolytic action of GH involves the Stat5 proteins while the insulin-like effects of GH on glucose metabolism and leptin release involve different mechanisms...- Most of the interleukin 1 receptor antagonist, cathepsin S, macrophage migration inhibitory factor, nerve growth factor, and interleukin 18 release by explants of human adipose tissue is by the non-fat cells, not by the adipocytesJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Metabolism 55:1113-21. 2006..Our results suggest that the non-fat cells in human adipose tissue contribute to most of the release of NGF, IL-18, IL-1Ra, cathepsin S, and MIF seen during primary culture of adipose tissue explants from obese women... - Transforming growth factor beta1 release by human adipose tissue is enhanced in obesityJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Metabolism 54:1546-51. 2005..The release of TGF-beta1 was primarily by the nonfat cells of human adipose tissue because release by adipocytes was less than 10% of that by the nonfat cells of adipose tissue... - Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humansJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
Endocrinology 145:2273-82. 2004..Furthermore, most of the adipokine release by the nonfat cells of adipose tissue was due to cells retained in the tissue matrix after collagenase digestion... - TNFalpha release by the nonfat cells of human adipose tissueJ N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Int J Obes Relat Metab Disord 28:616-22. 2004..The second aim was to examine the correlation between body mass index (BMI) and the subsequent release of adiponectin and TNFalpha by explants of human subcutaneous and visceral adipose tissue incubated in primary culture for 48 h... - Insulin enhances vascular endothelial growth factor, interleukin-8, and plasminogen activator inhibitor 1 but not interleukin-6 release by human adipocytesJohn N Fain
Department of Molecular Science, University of Tennessee Health Science Center, Memphis, TN 38163 3409, USA
Metabolism 54:220-6. 2005..The ability of insulin to stimulate VEGF formation by adipocytes suggests that the elevated circulating levels of insulin in obesity promote angiogenesis in adipose tissue as well as the enhanced accumulation of fat in human adipocytes... - Involvement of multiple signaling pathways in the post-bariatric induction of IL-6 and IL-8 mRNA and release in human visceral adipose tissueJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Biochem Pharmacol 69:1315-24. 2005..These cytokines, and probably other factors as well, enhance IL-6 and IL-8 mRNA accumulation in human adipose tissue explants through mechanisms involving the p38 MAPK and NFkappaB pathways... - Regulation of monocyte chemoattractant protein 1 (MCP-1) release by explants of human visceral adipose tissueJ N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Int J Obes (Lond) 29:1299-307. 2005..Monocyte chemoattractant protein-1 (MCP-1) is a chemokine involved in monocyte recruitment during inflammation whose plasma level is elevated in obesity... 
Release of 12 adipokines by adipose tissue, nonfat cells, and fat cells from obese womenJohn N Fain
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA
Obesity (Silver Spring) 18:890-6. 2010..In contrast that of ICAM-1, FABP-4, GPX-3, visfatin/PBEF/Nampt, CD14, lipocalin-2, LP, and endothelin-1 was significantly greater in tissue from women with a total fat mass of 65 kg...- Regulation of adiponectin release and demonstration of adiponectin mRNA as well as release by the non-fat cells of human omental adipose tissueJ N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 3816, USA
Int J Obes (Lond) 32:429-35. 2008.... - Release in vitro of adipsin, vascular cell adhesion molecule 1, angiotensin 1-converting enzyme, and soluble tumor necrosis factor receptor 2 by human omental adipose tissue as well as by the nonfat cells and adipocytesJohn N Fain
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Metabolism 56:1583-90. 2007..Furthermore, the enhanced release of VCAM-1 and ACE seen in omental adipose tissue explants as compared with explants of subcutaneous adipose tissue is due to release by nonfat cells... - Identification of omentin mRNA in human epicardial adipose tissue: comparison to omentin in subcutaneous, internal mammary artery periadventitial and visceral abdominal depotsJ N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Int J Obes (Lond) 32:810-5. 2008.... - Comparison of messenger RNA distribution for 60 proteins in fat cells vs the nonfat cells of human omental adipose tissueJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Metabolism 57:1005-15. 2008..We conclude that the mRNAs for inflammatory proteins are primarily present in the nonfat cells of human omental adipose tissue... - Haptoglobin release by human adipose tissue in primary cultureJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
J Lipid Res 45:536-42. 2004..However, the amount of haptoglobin released by human adipose tissue explants in primary culture was quite low in relationship to the circulating level of haptoglobin... - Regulation of leptin release and lipolysis by PGE2 in rat adipose tissueJ N Fain
Department of Biochemistry, College of Medicine, University of Tennessee, Memphis 38163, USA
Prostaglandins Other Lipid Mediat 62:343-50. 2000..However, while the endogenous formation of PGE2 does not appear to regulate basal lipolysis or leptin release, it may play a role in the activation of lipolysis by catecholamines... - Inhibition of leptin release by atrial natriuretic peptide (ANP) in human adipocytesJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Biochem Pharmacol 65:1883-8. 2003..Our data demonstrate that the lipolytic effect of ANP is probably secondary to stimulation of cyclic GMP accumulation in human adipocytes, and this is accompanied by an inhibition of leptin release... - Resistin release by human adipose tissue explants in primary cultureJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Biochem Biophys Res Commun 300:674-8. 2003..The present data suggest that resistin release by explants of human adipose tissue in primary culture is largely derived from the non-fat cells present in the explants... - Comparison of PGE2, prostacyclin and leptin release by human adipocytes versus explants of adipose tissue in primary cultureJ N Fain
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN, USA
Prostaglandins Leukot Essent Fatty Acids 67:467-73. 2002..In conclusion, these data indicate that leptin release is exclusively a function of adipocytes while prostanoids are made by both adipocytes and the other cells present in human adipose tissue.. - Obesity is induced in mice heterozygous for cyclooxygenase-2J N Fain
Department of Molecular Sciences, College of Medicine, The University of Tennessee Health Science Center, Memphis 38163, USA
Prostaglandins Other Lipid Mediat 65:199-209. 2001..These data indicate that heterozygous COX-2 mice develop obesity which is not secondary to a defect in leptin release by adipose tissue... - Abnormalities in the functioning of adipocytes from R6/2 mice that are transgenic for the Huntington's disease mutationJ N Fain
Departments of Molecular Sciences and Anatomy and Neurobiology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Hum Mol Genet 10:145-52. 2001..These data suggest that the obesity observed at 8-9 weeks in R6/2 mice may stem from a defect in fat breakdown by adipocytes... - Stimulation of leptin release by arachidonic acid and prostaglandin E(2) in adipose tissue from obese humansJ N Fain
Department of Biochemistry, College of Medicine, University of Tennessee, Memphis, TN 38163, USA
Metabolism 50:921-8. 2001..These data suggest that when upregulation of COX-2 is blocked by dexamethasone, exogenous PGE(2) enhances both leptin release and leptin mRNA accumulation by explants of human adipose tissue in primary culture... - Regulation of leptin release by insulin, glucocorticoids, G(i)-coupled receptor agonists, and pertussis toxin in adipocytes and adipose tissue explants from obese humans in primary cultureAlie Kanu
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38136, USA
Metabolism 52:60-6. 2003.... - Stimulation of leptin release by actinomycin D in rat adipocytesJ N Fain
Department of Biochemistry, College of Medicine, University of Tennessee at Memphis, 38163, USA
Biochem Pharmacol 55:1309-14. 1998..These results point to a unique regulation of leptin release and leptin mRNA levels by actinomycin D... - Regulation of oxytocin-induced phosphoinositide breakdown in adipocytes by adenosine, isoproterenol and insulinH M Lee
Department of Biochemistry, University of Tennessee, Memphis 38163
Biochim Biophys Acta 1013:73-9. 1989..These results indicate that, in adipocytes, phosphoinositide breakdown stimulated by oxytocin is enhanced by adenosine, inhibited by isoproterenol and, under some conditions is inhibited by insulin... - Stimulation of human omental adipose tissue lipolysis by growth hormone plus dexamethasoneJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Mol Cell Endocrinol 295:101-5. 2008..We conclude that the direct lipolytic effect of GH is accompanied by an increase in HSL mRNA in the presence of DEX, but GH also increased the mRNAs for other proteins that could explain all or part of its lipolytic action... - Site -2548 of the leptin gene is associated with gender-specific trends in newborn size and cord leptin levelsRonald M Adkins
Children s Foundation Research Center and Center of Genomics and Bioinformatics, University of Tennessee Health Science Center, Memphis, TN 38122, USA
Int J Pediatr Obes 2:130-7. 2007..We examined the relationships among leptin gene polymorphisms, size for gestational age, umbilical cord leptin, and gender... - Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levelsRonald M Adkins
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee 38103, USA
Pediatr Res 62:451-5. 2007..Analyses of haplotypes inferred from these two SNPs demonstrate a significant relationship between paternally transmitted haplotypes and newborn IGF-II levels, but no association with maternally transmitted haplotypes... - Are African-Americans as successful as Caucasians after laparoscopic gastric bypass?Atul K Madan
Section of Minimally Invasive Surgery, Department of Surgery, University of Tennessee Health Science Center, 956 Court Ave, Room G210, Memphis, TN 38163, USA
Obes Surg 17:460-4. 2007..Our hypothesis was that AAs are just as successful as CA after LRYGBP in terms of weight loss and comorbidity improvement... - Laparoscopic bariatric patients' will to help: the foundation of researchDavid S Tichansky
Section of Minimally Invasive Surgery, Department of Surgery, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
Surg Obes Relat Dis 3:180-3. 2007..Bariatric patient enthusiasm for research participation has never been objectively measured. Our hypothesis was that most laparoscopic bariatric surgery patients would participate in clinical research... - Comparison of IL-8, IL-6 and PGE(2) formation by visceral (omental) adipose tissue of obese Caucasian compared to African-American womenAtul K Madan
Department of Surgery, College of Medicine University of Tennessee Health Science Center, Memphis, TN 38163, USA
Obes Surg 16:1342-50. 2006..We hypothesized that there are ethnic differences in inflammatory markers and medical co-morbidities... - Dexamethasone and the inflammatory response in explants of human omental adipose tissueJohn N Fain
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Mol Cell Endocrinol 315:292-8. 2010..We conclude that dexamethasone is a selective anti-inflammatory agent since it inhibits only about one-fourth of the proteins up-regulated during in vitro incubation of human omental adipose tissue... - Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cellsJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
Vitam Horm 74:443-77. 2006..In conclusion, there is an increasing recognition that adipose tissue is an endocrine organ that secretes leptin and adiponectin along with a host of other paracrine and endocrine factors in addition to free fatty acids... - Human epicardial adipokine messenger RNAs: comparisons of their expression in substernal, subcutaneous, and omental fatJohn N Fain
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Metabolism 59:1379-86. 2010..The data do not support the hypothesis that most of the inflammatory adipokines are expressed at high levels in epicardial fat of humans... 
Release of inflammatory mediators by human adipose tissue is enhanced in obesity and primarily by the nonfat cells: a reviewJohn N Fain
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Mediators Inflamm 2010:513948. 2010..The conclusion is that the release of many inflammatory adipokines by adipose tissue is enhanced in obese humans...- Altered phosphoinositide-specific phospholipase C and adenylyl cyclase in brain cortical membranes of cats with GM1 and GM2 gangliosidosisE Claro
Department of Biochemistry, University of Tennessee, Memphis 38163
Brain Res Mol Brain Res 11:265-71. 1991..These data suggest that the activation of phosphoinositide-specific phospholipase C in brain membranes by guanine nucleotide binding proteins is markedly impaired in GM1 and GM2 gangliosidoses... - Human epicardial adipose tissue: a reviewHarold S Sacks
Division of Endocrinology and Metabolism, University of Tennessee, and Baptist Hospital Heart Institute, Memphis, TN, USA
Am Heart J 153:907-17. 2007..Further studies should be done to test the hypothesis that adipokines produced by and released from human epicardial adipose tissue might contribute locally to the pathogenesis of coronary atherosclerosis... - Uncoupling protein-1 and related messenger ribonucleic acids in human epicardial and other adipose tissues: epicardial fat functioning as brown fatHarold S Sacks
Department of Medicine, College of Medicine, University of Tennessee Health Science Center Memphis, Tennessee 38163, USA
J Clin Endocrinol Metab 94:3611-5. 2009..Uncoupling protein-1 (UCP-1) is the inner mitochondrial membrane protein that is a specific marker for and mediator of nonshivering thermogenesis in brown adipocytes... - Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissueJens M Bruun
Department of Endocrinology and Metabolism C, Aarhus Amtssygehus, Aarhus University Hospital and Faculty of Health Sciences, Aarhus University, Tage Hansensgade 2, DK 8000 Aarhus C, Denmark
Am J Physiol Endocrinol Metab 286:E8-13. 2004..The high levels of IL-8 release from human adipose tissue and accumulation of this tissue in obese subjects may account for some of the increase in circulating IL-8 observed in obesity...