John F Engelhardt


Affiliation: University of Iowa
Country: USA


  1. Sun X, Yi Y, Yan Z, Rosen B, Liang B, Winter M, et al. In utero and postnatal VX-770 administration rescues multiorgan disease in a ferret model of cystic fibrosis. Sci Transl Med. 2019;11: pubmed publisher
    ..Moreover, this ferret model provides proof of concept for in utero pharmacologic correction of genetic disease and offers opportunities for understanding CF pathogenesis and improving treatment. ..
  2. Sun X, Yi Y, Liang B, Yang Y, He N, Ode K, et al. Incretin dysfunction and hyperglycemia in cystic fibrosis: Role of acyl-ghrelin. J Cyst Fibros. 2019;: pubmed publisher
    ..Interventions that lower ghrelin, ghrelin action, and/or raise GLP-1 or GIP might improve glycemia in CF. ..
  3. Tyler S, Rotti P, Sun X, Yi Y, Xie W, Winter M, et al. PyMINEr Finds Gene and Autocrine-Paracrine Networks from Human Islet scRNA-Seq. Cell Rep. 2019;26:1951-1964.e8 pubmed publisher
    ..This proof-of-principle study demonstrates that the PyMINEr framework will be a valuable resource for scRNA-seq analyses. ..
  4. Yu M, Sun X, Tyler S, Liang B, Swatek A, Lynch T, et al. Highly Efficient Transgenesis in Ferrets Using CRISPR/Cas9-Mediated Homology-Independent Insertion at the ROSA26 Locus. Sci Rep. 2019;9:1971 pubmed publisher
    ..These methods and the model generated will greatly enhance biomedical research involving lineage tracing, the evaluation of stem cell therapy, and transgenesis in ferret models of human disease. ..
  5. Zou W, Xiong M, Deng X, Engelhardt J, Yan Z, Qiu J. A Comprehensive RNA-seq Analysis of Human Bocavirus 1 Transcripts in Infected Human Airway Epithelium. Viruses. 2019;11: pubmed publisher
    ..Thus, our study provides a systematic and unbiased transcription profile, including both mRNA and small RNA transcripts, of HBoV1 in HBoV1-infected HAE-ALI cultures. ..
  6. Lynch T, Anderson P, Rotti P, Tyler S, Crooke A, Choi S, et al. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium. Cell Stem Cell. 2018;22:653-667.e5 pubmed publisher
    ..Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. ..
  7. Rosen B, Evans T, Moll S, Gray J, Liang B, Sun X, et al. Infection is Not Required for Mucoinflammatory Lung Disease in CFTR-knockout Ferrets. Am J Respir Crit Care Med. 2018;: pubmed publisher
    ..These findings implicate mucoinflammatory processes in the CF lung as pathogenic in the absence of clinically apparent bacterial and fungal infections. ..
  8. Xie W, Lynch T, Liu X, Tyler S, Yu S, Zhou X, et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol. 2014;306:L645-60 pubmed publisher
    ..These studies support a model whereby Wnt signals and Sox2 dynamically regulate the expression of Lef-1 in airway epithelia and potentially also during SMG development. ..
  9. Sun X, Olivier A, Yi Y, Pope C, Hayden H, Liang B, et al. Gastrointestinal pathology in juvenile and adult CFTR-knockout ferrets. Am J Pathol. 2014;184:1309-22 pubmed publisher
    ..In summary, these studies demonstrate that the CF ferret model develops gastrointestinal pathology similar to CF patients. ..

More Information


  1. Rosen B, Chanson M, Gawenis L, Liu J, Sofoluwe A, Zoso A, et al. Animal and model systems for studying cystic fibrosis. J Cyst Fibros. 2018;17:S28-S34 pubmed publisher
    ..This short review evaluates several areas that are key to building animal and cell systems capable of modeling CF disease and testing potential treatments. ..
  2. Swatek A, Lynch T, Crooke A, Anderson P, Tyler S, Brooks L, et al. Depletion of Airway Submucosal Glands and TP63+KRT5+ Basal Cells in Obliterative Bronchiolitis. Am J Respir Crit Care Med. 2017;: pubmed publisher
    ..Global airway SC depletion may be a mechanism for pulmonary allograft failure. ..