Daniel A Engel

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc Yeast based small molecule screen for inhibitors of SARS-CoV
    Matthew Frieman
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America
    PLoS ONE 6:e28479. 2011
  2. pmc Novel inhibitor of influenza non-structural protein 1 blocks multi-cycle replication in an RNase L-dependent manner
    Marcin P Walkiewicz
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Gen Virol 92:60-70. 2011
  3. doi request reprint The influenza virus NS1 protein as a therapeutic target
    Daniel A Engel
    Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, United States Electronic address
    Antiviral Res 99:409-16. 2013
  4. pmc Novel influenza virus NS1 antagonists block replication and restore innate immune function
    Dipanwita Basu
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, 22908, USA
    J Virol 83:1881-91. 2009
  5. doi request reprint Design, synthesis, and evaluation of novel small molecule inhibitors of the influenza virus protein NS1
    Joseph J Jablonski
    University of Virginia, Department of Chemistry, Charlottesville, VA 22904, USA
    Bioorg Med Chem 20:487-97. 2012
  6. pmc Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles
    Marcin P Walkiewicz
    Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
    J Virol Methods 159:251-8. 2009
  7. ncbi request reprint Transcription releases protein VII from adenovirus chromatin
    Jiangning Chen
    Department of Microbiology, University of Virginia Health System, PO Box 800734, Charlottesville, VA 22908 0734, USA
    Virology 369:411-22. 2007
  8. pmc Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32
    Yuming Xue
    Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
    J Virol 79:2474-83. 2005
  9. pmc Adenovirus protein VII condenses DNA, represses transcription, and associates with transcriptional activator E1A
    Jeffrey S Johnson
    Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908, USA
    J Virol 78:6459-68. 2004
  10. doi request reprint Insertion of CTCF-binding sites into a first-generation adenovirus vector reduces the innate inflammatory response and prolongs transgene expression
    Jerome Schaack
    Department of Microbiology, University of Colorado Medical School, Aurora, CO 80045, USA
    Virology 412:136-45. 2011

Research Grants

Collaborators

Detail Information

Publications13

  1. pmc Yeast based small molecule screen for inhibitors of SARS-CoV
    Matthew Frieman
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America
    PLoS ONE 6:e28479. 2011
    ....
  2. pmc Novel inhibitor of influenza non-structural protein 1 blocks multi-cycle replication in an RNase L-dependent manner
    Marcin P Walkiewicz
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Gen Virol 92:60-70. 2011
    ..This represents a new direction for anti-influenza virus drug development that exploits the IFN pathway to challenge virus replication...
  3. doi request reprint The influenza virus NS1 protein as a therapeutic target
    Daniel A Engel
    Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, United States Electronic address
    Antiviral Res 99:409-16. 2013
    ..This review describes biochemical, cell-based and nucleic acid-based approaches to identifying NS1 antagonists. ..
  4. pmc Novel influenza virus NS1 antagonists block replication and restore innate immune function
    Dipanwita Basu
    Department of Microbiology, University of Virginia School of Medicine, Charlottesville, 22908, USA
    J Virol 83:1881-91. 2009
    ..These data demonstrate that the function of NS1 can be modulated by chemical inhibitors and that such inhibitors will be useful as probes of biological function and as starting points for clinical drug development...
  5. doi request reprint Design, synthesis, and evaluation of novel small molecule inhibitors of the influenza virus protein NS1
    Joseph J Jablonski
    University of Virginia, Department of Chemistry, Charlottesville, VA 22904, USA
    Bioorg Med Chem 20:487-97. 2012
    ..Results suggest that the target binding site tolerates hydrophobic bulk as well as having a preference for weakly basic substituents...
  6. pmc Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles
    Marcin P Walkiewicz
    Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
    J Virol Methods 159:251-8. 2009
    ..The method can be applied to any adenovirus vector and gives results equivalent to the standard plaque assay...
  7. ncbi request reprint Transcription releases protein VII from adenovirus chromatin
    Jiangning Chen
    Department of Microbiology, University of Virginia Health System, PO Box 800734, Charlottesville, VA 22908 0734, USA
    Virology 369:411-22. 2007
    ..Our data support a model in which early gene transcription results in a change in the structure of the viral chromatin...
  8. pmc Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32
    Yuming Xue
    Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
    J Virol 79:2474-83. 2005
    ..Interestingly, disappearance of the dots required ongoing RNA synthesis but not DNA synthesis. Taken together these data indicate that protein VII has an ongoing role during early phase and the beginning of DNA replication...
  9. pmc Adenovirus protein VII condenses DNA, represses transcription, and associates with transcriptional activator E1A
    Jeffrey S Johnson
    Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908, USA
    J Virol 78:6459-68. 2004
    ..These studies define the in vivo functions of protein VII in DNA binding, condensation, and transcriptional repression and indicate a role in E1A-mediated transcriptional activation of viral genes...
  10. doi request reprint Insertion of CTCF-binding sites into a first-generation adenovirus vector reduces the innate inflammatory response and prolongs transgene expression
    Jerome Schaack
    Department of Microbiology, University of Colorado Medical School, Aurora, CO 80045, USA
    Virology 412:136-45. 2011
    ....
  11. doi request reprint An integrated, valveless system for microfluidic purification and reverse transcription-PCR amplification of RNA for detection of infectious agents
    Kristin A Hagan
    Department of Chemistry, University of Virginia, McCormick Road, P O Box 400319, Charlottesville, VA 22904, USA
    Lab Chip 11:957-61. 2011
    ..This was initially accomplished on a two-chip system to optimize the SPE and RT-PCR, and then translated to an integrated SPE-RT-PCR device...
  12. ncbi request reprint Adenovirus type 5 DNA-protein complexes from formaldehyde cross-linked cells early after infection
    David J Spector
    Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Virology 312:204-12. 2003
    ..The S component probably contained uncoated nuclear viral DNA that assembles into early viral transcription complexes...
  13. ncbi request reprint Adenovirus E1A requires the yeast SAGA histone acetyltransferase complex and associates with SAGA components Gcn5 and Tra1
    Caroline A Kulesza
    Department of Microbiology and Cancer Center, University of Virginia School of Medicine, Charlottesville, Virginia, VA 22908, USA
    Oncogene 21:1411-22. 2002
    ..These results indicate a strong requirement for intact SAGA complex for E1A to function in yeast, and suggest a role for SAGA-like complexes in mammalian cell transformation...

Research Grants11

  1. Development of Yeast-Based Assays for anti-influenza drug discovery
    Daniel Engel; Fiscal Year: 2006
    ..Again using our pdr1/pdr3 genetic background we will establish expression conditions for PKR-induced toxicity and p58 inhibition of PKR. This system will be used to establish microtiter plate assays and robotics screening. ..
  2. ADENOVIRUS E1A PROTEIN AND THE SWI/SNF COMPLEX
    Daniel Engel; Fiscal Year: 2005
    ..The yeast proteins that interact with E1A will be identified and their influence on E1A activity will be determined. Similar studies will then be conducted in mammalian cells using the information gained from the yeast system. ..
  3. Novel Tool Compounds for Chromatin Research
    Daniel Engel; Fiscal Year: 2004
    ..These two areas will be the main thrust of the future development of this project. ..
  4. CHROMATIN TARGETS FOR CANCER THERAPY
    Daniel Engel; Fiscal Year: 2002
    ..Lead compounds will be tested further in mammalian cell culture systems to examine their potential as therapeutic agents, and their specific effects on mammalian chromatin dynamics. ..