Research Topics
Genomes and GenesSpecies | Andrew EllingtonSummaryAffiliation: University of Texas Country: USA Publications
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Publications
Deoxyribozymes that recode sequence informationJeffrey J Tabor
Center for Systems and Synthetic Biology and Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Nucleic Acids Res 34:2166-72. 2006..The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs...
Spatial control of DNA reaction networks by DNA sequencePeter B Allen
Institute of Cell and Molecular Biology, University of Texas at Austin, 1 University Station A4800, Austin, TX 78712 0159, USA
Molecules 17:13390-402. 2012..The ability to program at the nanoscale so as to produce patterns at the macroscale is a step towards programmable, synthetic chemical systems for generating defined spatiotemporal patterns...- Coupling two different nucleic acid circuits in an enzyme-free amplifierYu Jiang
Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Molecules 17:13211-20. 2012..The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal... - DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platformPeter B Allen
University of Texas at Austin, Chemistry and Biochemistry Dept, 1 University Station, A4800, USA
Lab Chip 12:2951-8. 2012..By bridging the gap between the low concentrations of LAMP amplicons and the limits of fluorescence detection, the non-enzymatic amplifier allowed us to detect as few as 1200 input templates in a paperfluidic format... - Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genesRammohan Narayanaswamy
Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA
Genome Biol 7:R6. 2006..Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging... - Anticipatory evolution and DNA shufflingJamie M Bacher
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Genome Biol 3:REVIEWS1021. 2002..A mix of theoretical and applied research has now provided insights into how recombination can be guided to more efficiently generate proteins and even organisms with altered functions... - Evolution of phage with chemically ambiguous proteomesJamie M Bacher
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
BMC Evol Biol 3:24. 2003..The evolution of a small bacteriophage proteome to accommodate an unnatural amino acid analogue can provide insights into the number and type of substitutions that individual proteins will require to retain functionality... - A combined in vitro/in vivo selection for polymerases with novel promoter specificitiesJ Chelliserrykattil
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, USA
BMC Biotechnol 1:13. 2001..This method may have applications for evolving other polymerase variants with novel phenotypes, such as the ability to incorporate modified nucleotides... - Group I aptazymes as genetic regulatory switchesKristin M Thompson
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
BMC Biotechnol 2:21. 2002..We attempted to generate Group I self-splicing introns that were activated by a small organic effector, theophylline, and to show that such Group I aptazymes could mediate theophylline-dependent splicing in vivo... - The scene of a frozen accidentA D Ellington
Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA
RNA 6:485-98. 2000..We argue that the suggested connections between modern motifs and ancient sequences are logically tenuous, and show that there is no statistically meaningful association between motifs found in aptamers and codons... - Evolutionary origins and directed evolution of RNAAndrew D Ellington
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, United States
Int J Biochem Cell Biol 41:254-65. 2009..Self-replication would have inexorably led to life... - Man versus machine versus ribozymeAndrew D Ellington
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas, United States of America
PLoS Biol 6:e132. 2008 
What's so great about RNA?Andrew D Ellington
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
ACS Chem Biol 2:445-8. 2007- Optimization of the biological component of a bioelectrochemical cellEun Jeong Cho
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Bioelectrochemistry 70:165-72. 2007..We are further exploring whether this increase was due to metabolic adaptations or to genetic mutations, and examining additional ways to evolve electrogenic organisms... - Ribozyme-mediated signal augmentation on a mass-sensitive biosensorScott M Knudsen
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
J Am Chem Soc 128:15936-7. 2006..Aptazyme activity was observed in real time, and low-molecular-weight analyte detection has been successfully demonstrated with both aptazymes... - Applications of aptamers as sensorsEun Jeong Cho
The Institute for Drug and Diagnostic Development, University of Texas at Austin, Austin, Texas 78712, USA
Annu Rev Anal Chem (Palo Alto Calif) 2:241-64. 2009..However, application of aptamers without a basic knowledge of their biochemistry or technical requirements can cause serious analytical difficulties... - Evolution. Changing the cofactor diet of an enzymeAndrew D Ellington
Departments of Chemistry and Integrative Biology, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Science 310:454-5. 2005 - The robustness of naturally and artificially selected nucleic acid secondary structuresLauren Ancel Meyers
Section of Integrative Biology, University of Texas at Austin, 1 University Station C0930, Austin, TX 78712, USA
J Mol Evol 58:681-91. 2004..The thermostability of RNA molecules bred in the laboratory is probably not constrained by a lack of suitable variation in the sequence pool but, rather, by intrinsic biases in the selection process... - Motifs from the deepTony W Hwang
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas, Austin, TX78712, USA
J Biol 8:72. 2009.... - Synthetic RNA circuitsEric A Davidson
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, 1 University Station A4800, University of Texas at Austin, Austin, Texas 78712, USA
Nat Chem Biol 3:23-8. 2007.... - Direct selection of trans-acting ligase ribozymes by in vitro compartmentalizationMatthew Levy
Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78751, USA
RNA 11:1555-62. 2005.... - Ribozyme catalysis of metabolism in the RNA worldXi Chen
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Chem Biodivers 4:633-55. 2007.... - In vitro selection using modified or unnatural nucleotidesScott M Knudsen
University of Texas, Austin, Texas, USA
Curr Protoc Nucleic Acid Chem . 2002..It includes a discussion of when to use modified nucleotides; protocols for preparing a modified RNA pool and verifying its suitability for in vitro selection; and protocols for selecting and amplifying a functionally enriched pool... - Developing RNA tools for engineered regulatory systemsJeffrey J Tabor
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712-0159, USA
Biotechnol Genet Eng Rev 22:21-44. 2006 - Engineering antibody fragments to fold in the absence of disulfide bondsMin Jeong Seo
Department of Chemical Engineering, University of Texas, Austin, 78712, USA
Protein Sci 18:259-67. 2009..Using this approach, we isolated scFv antibody variants that are fully active when expressed in the cytoplasm or when the four Cys residues that normally form disulfides are substituted by Ser residues... - Design principles for ligand-sensing, conformation-switching ribozymesXi Chen
Department of Chemistry and Biochemistry, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA
PLoS Comput Biol 5:e1000620. 2009..The robust theoretical framework and identified optimization parameters should now enable the precision design of aptazymes for biotechnological and clinical applications... - Engineering regulatory RNAsEric A Davidson
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Trends Biotechnol 23:109-12. 2005..A recent paper by Isaacs et al. describes the engineering and in vivo activity of a small RNA that removes translation inhibition by binding the 5' untranslated region of its target mRNA and making the ribosome-binding site accessible... - Simultaneous detection of diverse analytes with an aptazyme ligase arrayJay R Hesselberth
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
Anal Biochem 312:106-12. 2003.... - ATP-dependent allosteric DNA enzymesMatthew Levy
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Chem Biol 9:417-26. 2002..This novel allosteric mechanism has not previously been observed for nucleic-acid catalysts and is rare even in protein catalysts... - Peptide-templated nucleic acid ligationMatthew Levy
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, 78712, USA
J Mol Evol 56:607-15. 2003..These results support the possibility that life could have originated with peptide replicators and transitioned to nucleic acid replicators or that peptide and nucleic acid replicators could have been interdependent... - Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repressionBrad Reveal
Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, TX, USA
RNA Biol 8:1047-60. 2011..Other proteins with multiple RRMs may employ combinatorial binding to achieve high levels of specificity and affinity... - Arginine-rich motifs present multiple interfaces for specific binding by RNATravis S Bayer
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
RNA 11:1848-57. 2005.... - Aptamer databaseJennifer F Lee
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, USA
Nucleic Acids Res 32:D95-100. 2004..The database is updated monthly and is publicly available at http://aptamer. icmb.utexas.edu/... - Engineering stochasticity in gene expressionJeffrey J Tabor
Center for Systems and Synthetic Biology and Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA
Mol Biosyst 4:754-61. 2008..These results demonstrate that synthetic genetic constructs can significantly affect the noise profile of a living cell and, importantly, that operons are a facile genetic strategy for buffering against noise... - Bioinformatic analysis of the contribution of primer sequences to aptamer structuresMatthew C Cowperthwaite
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
J Mol Evol 67:95-102. 2008.... - Modelling amorphous computations with transcription networksZack Booth Simpson
Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
J R Soc Interface 6:S523-33. 2009..1038/msb4100099)). The hairpin transcriptional gates are uniquely suited to the design of a complementary NAND gate that can serve as an underlying basis of molecular computing that can output matter rather than electronic information... - Aptamers as potential diagnostic reagents for diabetesAmy Yan
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
Diabetes Technol Ther 4:339-46. 2002 - AANT: the Amino Acid-Nucleotide Interaction DatabaseMichael M Hoffman
Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712-0159, USA
Nucleic Acids Res 32:D174-81. 2004..Moreover, by modularly representing the fundamental interactions that govern binding specificity it may prove possible to better engineer nucleic acid binding proteins... - Selecting nucleic acids for biosensor applicationsManjula Rajendran
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA
Comb Chem High Throughput Screen 5:263-70. 2002..Nucleic acid biosensor arrays for non-nucleic acid targets could likely be generated with the same facility as DNA chips... - Increasing the thermal stability of an oligomeric protein, beta-glucuronidaseHumberto Flores
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology ICMB a4800 MBB 3 424, University of Texas at Austin, 26th and Speedway, Austin, TX 78712, USA
J Mol Biol 315:325-37. 2002.... - Functional RNA microarrays for high-throughput screening of antiprotein aptamersJames R Collett
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Anal Biochem 338:113-23. 2005..Integrating RNA aptamer microarray production with the maturing technology for automated in vitro selection of antiprotein aptamers should result in the high-throughput production of proteome chips... - Tuning the specificity of a synthetic receptor using a selected nucleic acid receptorJoseph C Manimala
The University of Texas at Austin, Institute for Cellular and Molecular Biology. 1 University Station, A5300, University of Texas, Austin, Texas 78712, USA
J Am Chem Soc 126:16515-9. 2004..The RNA conformation changed upon the introduction of the synthetic host, consistent with an induced-fit mechanism for binding... - Direct selection for ribozyme cleavage activity in cellsXi Chen
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
RNA 15:2035-45. 2009..More importantly, these results have led us to develop a quantitative, kinetic model that can be used to assess the stringency of the hybrid selection scheme and to direct future experiments... - Feature multiplexing--improving the efficiency of microarray devicesMatthew J Schmid
Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78712, USA
Angew Chem Int Ed Engl 45:3338-41. 2006 - Kinetic optimization of a protein-responsive aptamer beaconBradley Hall
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
Biotechnol Bioeng 103:1049-59. 2009..By integrating these various interactions, we were ultimately able to design aptamer beacons that were activated by threefold within 1 min of the addition of thrombin... - Technical and biological issues relevant to cell typing with aptamersNa Li
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
J Proteome Res 8:2438-48. 2009..Better understanding and controlling for the role of background and nonspecific binding to cells should open the way to using arrays of aptamers for describing and quantifying the cell surface proteome... - Automated selection of aptamers against protein targets translated in vitro: from gene to aptamerJ Colin Cox
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Nucleic Acids Res 30:e108. 2002..These results not only reveal a potential path to the high throughput generation of aptamers, but also yield insights into the incredible specificity of the U1A protein for its natural RNA ligands... - Using RNA aptamers and the proximity ligation assay for the detection of cell surface antigensSupriya S Pai
Department of Microbiology, University of Texas at Austin, Austin, TX, USA
Methods Mol Biol 504:385-98. 2009.... - Aptamers that recognize drug-resistant HIV-1 reverse transcriptaseNa Li
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Nucleic Acids Res 36:6739-51. 2008..M3 and WT HIV-1 RTs could be distinguished using an aptamer-based microarray. By probing protein conformation as a correlate to drug resistance we introduce an additional and useful measure for determining HIV-1 drug resistance... - Directed evolution of proteins in vitro using compartmentalization in emulsionsEric A Davidson
University of Texas at Austin, Austin, Texas, USA
Curr Protoc Mol Biol . 2009.... - Production and processing of aptamer microarraysJames R Collett
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Methods 37:4-15. 2005.... - Design, synthesis, and amplification of DNA pools for in vitro selectionBradley Hall
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA
Curr Protoc Mol Biol . 2009..Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions... - In vitro selection of RNA aptamers to a protein target by filter immobilizationBradley Hall
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA
Curr Protoc Mol Biol . 2009..Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription... - Electrospray ionization of nucleic acid aptamer/small molecule complexes for screening aptamer selectivityKarin M Keller
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin TX 78712, USA
J Mass Spectrom 40:1327-37. 2005..These results indicate that in at least some cases, mass spectrometric data on aptamer/ligand binding affinities should be used in conjunction with complementary techniques to fully assess aptamer molecular recognition properties... - Infrared multiphoton dissociation of small-interfering RNA anions and cationsMyles W Gardner
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712 0165, USA
J Am Soc Mass Spectrom 21:580-91. 2010..With longer irradiation times, however, the individual single-strands underwent secondary dissociation to yield informative sequence ions not obtained by CID... - Mistakes in translation don't translate into terminationRandall A Hughes
Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
Proc Natl Acad Sci U S A 102:1273-4. 2005 - Using a deoxyribozyme ligase and rolling circle amplification to detect a non-nucleic acid analyte, ATPEun Jeong Cho
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
J Am Chem Soc 127:2022-3. 2005..Cooperative ATP activation of the deoxyribozyme was faithfully mimicked by RCA, yielding an amplified "switch" that was responsive to ATP concentration... - Autogene selectionsJijumon Chelliserrykattil
Department of Chemistry and Biochemistry, University of Texas, Austin, TX, USA
Methods Mol Biol 230:27-43. 2003 - The importance of prebiotic chemistry in the RNA worldRandall A Hughes
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Curr Opin Chem Biol 8:629-33. 2004..4 billion years ago are tenuous at best. Thus, perhaps the best way to understand origins is not by examining relatively unconstrained sequence information, but by examining the inherent constraints imposed by prebiotic chemistry... - Computational selection of nucleic acid biosensors via a slip structure modelBradley Hall
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Biosens Bioelectron 22:1939-47. 2007.... - Aptamer-based sensor arrays for the detection and quantitation of proteinsRomy Kirby
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
Anal Chem 76:4066-75. 2004..The aptamer chips proved to be useful for screening aptamers from in vitro selection experiments and for sensitively quantitating the biothreat agent ricin... - Using aptamers to identify and enter cellsTed Chu
University of Texas at Austin, Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, Austin, TX 78712, USA
Curr Opin Mol Ther 9:137-44. 2007..The generation of anti-cell aptamers has important implications for identifying disease-specific biomarkers, generating diagnostics, and developing novel drug delivery strategies... - In vitro selection of molecular beaconsManjula Rajendran
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Nucleic Acids Res 31:5700-13. 2003..The ability to select molecular beacons may prove useful for identifying available sites on complex targets, such as mRNAs, while the method for selection can be easily generalized to other, non-nucleic acid target classes... - Selection of fluorescent aptamer beacons that light up in the presence of zincManjula Rajendran
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA
Anal Bioanal Chem 390:1067-75. 2008..Such biosensors could potentially be used for continuous monitoring of metals in environmental samples... - Exponential growth by cross-catalytic cleavage of deoxyribozymogensMatthew Levy
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas, Austin 78712, USA
Proc Natl Acad Sci U S A 100:6416-21. 2003..Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes... - Quantum-dot aptamer beacons for the detection of proteinsMatthew Levy
Institute for Cellular and Molecular Biology, University of Texas, Austin, Austin TX 78712, USA
Chembiochem 6:2163-6. 2005 - Real-time PCR detection of protein analytes with conformation-switching aptamersLitao Yang
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Anal Biochem 380:164-73. 2008..The method has advantages relative to both immunoPCR (because no signal is produced by background binding) and the proximity ligation assay (PLA) (because only one epitope, rather than two epitopes, on a protein surface must be bound)... - In vitro selection of ribozymes dependent on peptides for activityMichael P Robertson
University of California-Santa Cruz, Santa Cruz, CA 95064, USA
RNA 10:114-27. 2004..These results have important implications for the development of aptazymes that can be used in arrays for the detection and quantitation of multiple cellular proteins (proteome arrays)... - A modified consensus approach to mutagenesis inverts the cofactor specificity of Bacillus stearothermophilus lactate dehydrogenaseHumberto Flores
, Apartado Postal 510-3, Cuernavaca, Morelos, 62271,
Protein Eng Des Sel 18:369-77. 2005.... - Q &A. Andrew D. EllingtonAndrew D Ellington
Curr Biol 18:R184-5. 2008 - Ribozyme déjà vuScott M Knudsen
Nat Struct Mol Biol 11:301-3. 2004 - Binding of herpes simplex virus-1 US11 to specific RNA sequencesKevin F Bryant
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
Nucleic Acids Res 33:6090-100. 2005..The results define a relatively short specific sequence that binds US11 with high affinity and indicate that dsRNA alone does not confer high-affinity binding... - Global incorporation of unnatural amino acids in Escherichia coliJamie M Bacher
The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA, USA
Methods Mol Biol 352:23-34. 2007..Here we describe protocols to characterize the effects of such proteome-wide perturbations... - A (ribo) switch in the paradigms of genetic regulationJay R Hesselberth
Nat Struct Biol 9:891-3. 2002 - Evolution of a T7 RNA polymerase variant that transcribes 2'-O-methyl RNAJijumon Chelliserrykattil
Nat Biotechnol 22:1155-60. 2004..Most important, our method allows the selection of polymerases that have good processivities and can be combined to simultaneously incorporate several different modified nucleotides in a transcript... - Synthetic biology: engineering Escherichia coli to see lightAnselm Levskaya
Biophysics Program, University of California, San Francisco, California 94143, USA
Nature 438:441-2. 2005..This spatial control of bacterial gene expression could be used to 'print' complex biological materials, for example, and to investigate signalling pathways through precise spatial and temporal control of their phosphorylation steps... - Protein-dependent ribozymes report molecular interactions in real timeJörg S Hartig
Kekule Institut fur Organische Chemie und Biochemie, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
Nat Biotechnol 20:717-22. 2002..The rapid identification of interactions between proteins or of compounds that disrupt such interactions should have substantial utility for the drug-discovery process...
Research Grants
- MicroRNA Profiles of Pathogen InfectionAndrew Ellington; Fiscal Year: 2005..3. Apply these methods to the detection of changes in microRNA expression during influenza infection. 4. Apply these methods to the detection of changes in microRNA expression with other infectious agents. ..
- T7 RNA polymerase engineering and RNA amplificationAndrew Ellington; Fiscal Year: 2007..This will enable microarray gene analysis with as little as 1 ng of total RNA with a single round of amplification or microarray profiling from a single cell (-10 pg of total RNA) after two rounds of amplification. ..
- Transducing Tumor Cell Antigens to AmpliconsAndrew Ellington; Fiscal Year: 2006..abstract_text> ..
- Genetic circuits based on allosteric ribozymesAndrew Ellington; Fiscal Year: 2007....
- Design and Selection of Aptamer BeaconsAndrew Ellington; Fiscal Year: 2007..abstract_text> ..
- Site-specific incorporation of FRET pairs into intracellular proteinsAndrew Ellington; Fiscal Year: 2009..This will allow us to follow and track proteins in a cell, and to determine where, when, and how proteins reside next to one another, in either normal or malformed complexes. ..
- Genetic circuits based on allosteric ribozymesAndrew D Ellington; Fiscal Year: 2010....
- Amorphous computation with transcription logic gatesAndrew D Ellington; Fiscal Year: 2010..The results of these inquiries should help understand development, including how development sometimes goes awry during disease formation, and may assist with building nanoscale devices for therapy and diagnostics. ..
- APTAMER BASED DETECTOR TO QUANTIFY MACROMOLECULESAndrew Ellington; Fiscal Year: 2006....
- REV DECOYS FOR GENE THERAPY AND DRUG DEVELOPMENTAndrew Ellington; Fiscal Year: 1999..In particular, the peptide motifs identified by physical mapping and selection studies should provide information essential to the synthesis of peptidomimetic drugs. ..
- REV DECOYS FOR GENE THERAPY AND DRUG DEVELOPMENTAndrew Ellington; Fiscal Year: 2004....
- Auto Selection of aptamers binding to pX01 proteomeAndrew Ellington; Fiscal Year: 2004..abstract_text> ..
- Ribozymes for Peptide- and Protein- Sensing Chip ArraysAndrew Ellington; Fiscal Year: 2004..1.-D.3), and (2)Adapting peptide and protein-activated aptazymes to function in chip arrays (D.4). ..
- DNA circuits for point-of-care diagnosticsAndrew D Ellington; Fiscal Year: 2010..The selfsame DNA circuitry can be readily redesigned for the detection of other mRNAs and alleles, and should therefore find great use in first world clinical settings, as well. ..