E Egelman

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. doi request reprint Helicity in electron microscopy images-a comment on Wang TF, Chen LT and Wang AH 2008 BioEssays 30:48-56
    Edward H Egelman
    Bioessays 30:791-2. 2008
  2. pmc Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits
    V E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Cell Biol 153:75-86. 2001
  3. ncbi request reprint A robust algorithm for the reconstruction of helical filaments using single-particle methods
    E H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Health Sciences Center, Charlottesville 22908 0733, USA
    Ultramicroscopy 85:225-34. 2000
  4. ncbi request reprint A tale of two polymers: new insights into helical filaments
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908 0733, USA
    Nat Rev Mol Cell Biol 4:621-30. 2003
  5. ncbi request reprint Actin's prokaryotic homologs
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Box 800733, Charlottesville 22908 0733, USA
    Curr Opin Struct Biol 13:244-8. 2003
  6. pmc Single-particle reconstruction from EM images of helical filaments
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908 0733, USA
    Curr Opin Struct Biol 17:556-61. 2007
  7. ncbi request reprint The iterative helical real space reconstruction method: surmounting the problems posed by real polymers
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, P O Box 800733, Charlottesville, VA 22908 0733, USA
    J Struct Biol 157:83-94. 2007
  8. doi request reprint Electron microscopy of helical filaments: rediscovering buried treasures in negative stain
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    Bioessays 31:909-11. 2009
  9. ncbi request reprint Does a stretched DNA structure dictate the helical geometry of RecA-like filaments?
    E H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 309:539-42. 2001
  10. ncbi request reprint Molecular evolution: actin's long lost relative found
    E H Egelman
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0733, USA
    Curr Biol 11:R1022-4. 2001

Collaborators

Detail Information

Publications68

  1. doi request reprint Helicity in electron microscopy images-a comment on Wang TF, Chen LT and Wang AH 2008 BioEssays 30:48-56
    Edward H Egelman
    Bioessays 30:791-2. 2008
  2. pmc Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits
    V E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Cell Biol 153:75-86. 2001
    ..All of these states display a high degree of cooperativity that appears to be an integral part of F-actin...
  3. ncbi request reprint A robust algorithm for the reconstruction of helical filaments using single-particle methods
    E H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Health Sciences Center, Charlottesville 22908 0733, USA
    Ultramicroscopy 85:225-34. 2000
    ..The method can be applied transparently to structures where Bessel overlap would greatly complicate helical analysis. In addition, the procedure allows for the ab initio determination of helical symmetry, when no prior knowledge exists...
  4. ncbi request reprint A tale of two polymers: new insights into helical filaments
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908 0733, USA
    Nat Rev Mol Cell Biol 4:621-30. 2003
    ..General features of polymorphism, cooperativity and allostery that emerge from studies of eukaryotic actin and bacterial RecA raise more general issues about how conserved these filamentous structures have been during evolution...
  5. ncbi request reprint Actin's prokaryotic homologs
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Box 800733, Charlottesville 22908 0733, USA
    Curr Opin Struct Biol 13:244-8. 2003
    ..It is possible that the interesting properties of eukaryotic F-actin may account for the unusual degree of conservation among the actins, whereas the bacterial proteins have had fewer constraints over the course of evolution...
  6. pmc Single-particle reconstruction from EM images of helical filaments
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908 0733, USA
    Curr Opin Struct Biol 17:556-61. 2007
    ..Applications of these new methods to viruses, actin filaments, pili and many other polymers show the great advantages of the new methods...
  7. ncbi request reprint The iterative helical real space reconstruction method: surmounting the problems posed by real polymers
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, P O Box 800733, Charlottesville, VA 22908 0733, USA
    J Struct Biol 157:83-94. 2007
    ..The problems inherent in the traditional approach are discussed, and examples are presented illustrating how the IHRSR approach surmounts these problems...
  8. doi request reprint Electron microscopy of helical filaments: rediscovering buried treasures in negative stain
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    Bioessays 31:909-11. 2009
    ..The failure to appreciate this has recently led to an incorrect interpretation of RecA-family filament structures...
  9. ncbi request reprint Does a stretched DNA structure dictate the helical geometry of RecA-like filaments?
    E H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 309:539-42. 2001
    ..In this view, these proteins stabilize this existing state of DNA, rather than induce a novel conformation...
  10. ncbi request reprint Molecular evolution: actin's long lost relative found
    E H Egelman
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0733, USA
    Curr Biol 11:R1022-4. 2001
    ..While we still know little about MreB's function, the structural similarities and differences between MreB and actin provide more insight into the remarkable properties of actin...
  11. doi request reprint Reducing irreducible complexity: divergence of quaternary structure and function in macromolecular assemblies
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    Curr Opin Cell Biol 22:68-74. 2010
    ..Work on the enzyme FucU, involved in fucose metabolism, may suggest similar conclusions...
  12. ncbi request reprint Bacterial conjugation: running rings around DNA
    E H Egelman
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    Curr Biol 11:R103-5. 2001
    ..An integral membrane bacterial protein assembly involved in the transfer of DNA between cells has been shown to resemble a ring helicase, suggesting that it hydrolyzes ATP to pump DNA through a central channel...
  13. ncbi request reprint The location of ubiquitin in Lethocerus arthrin
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Jordan Hill Box 800773, Charlottesville, VA 22908 0733, USA
    J Mol Biol 325:623-8. 2003
    ..This might be the basis for a destabilization of the on state at rest length. Lys118 is the most likely residue to which the ubiquitin is conjugated, based upon fitting atomic structures of actin and ubiquitin into the reconstruction...
  14. ncbi request reprint The bacterial protein SipA polymerizes G-actin and mimics muscle nebulin
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Nat Struct Biol 9:518-21. 2002
    ..We suggest that SipA is a bacterial structural mimic of muscle nebulin and nebulin-like proteins in non-muscle cells that are involved in the regulation of the actin-based cytoskeleton...
  15. pmc Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization
    Albina Orlova
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    Proc Natl Acad Sci U S A 101:17664-8. 2004
    ..These observations suggest that F-actin filaments are annealed over time into a stable filament and that actin-depolymerizing proteins can effect a time reversal of polymerization...
  16. ncbi request reprint Do the utrophin tandem calponin homology domains bind F-actin in a compact or extended conformation?
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Jordan Hill Box 800773, Charlottesville, VA 22908 0733, USA
    J Mol Biol 331:967-72. 2003
    ..We conclude that helical averaging applied to disordered filaments is responsible for their results, and that approaches designed to separate out homogeneous subsets within such filamentous complexes offer many advantages...
  17. ncbi request reprint Each actin subunit has three nebulin binding sites: implications for steric blocking
    Natalya Lukoyanova
    Department of Biochemistry, University of Virginia, Charlottesville 22908 0733, USA
    Curr Biol 12:383-8. 2002
    ..Muscle regulation involves tropomyosin movement on the surface of actin, with binding in three states. Our results suggest the intriguing possibility that intact nebulin may also be able to occupy three different sites on F-actin...
  18. ncbi request reprint The hexameric ring structure of the Escherichia coli RuvB branch migration protein
    Yen Ju Chen
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, Box 800733, Charlottesville, VA 22908 0733, USA
    J Mol Biol 319:587-91. 2002
    ..This suggests that there may be a highly conserved structure for many proteins involved in different aspects of DNA replication, recombination, transcription and repair...
  19. pmc Structural polymorphism of the ParM filament and dynamic instability
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    Structure 17:1253-64. 2009
    ..Our results suggest that the closed state of the cleft is required but not sufficient for ParM polymerization, and provide a structural basis for the dynamic instability of ParM filaments...
  20. ncbi request reprint Issues of resolution and polymorphism in single-particle reconstruction
    Shixin Yang
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Struct Biol 144:162-71. 2003
    ..These averages, however, are themselves artefactual as they involve heterogeneous images. The issues presented here need to be considered when single-particle EM reconstructions are evaluated...
  21. ncbi request reprint The CH-domain of calponin does not determine the modes of calponin binding to F-actin
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Mol Biol 359:478-85. 2006
    ..This has broad implications for inferring function from the presence of structurally conserved domains...
  22. pmc The structure of bacterial ParM filaments
    Albina Orlova
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908 0733, USA
    Nat Struct Mol Biol 14:921-6. 2007
    ..The present results yield new insights into polymorphisms within F-actin, as well as the evolution of polymer families...
  23. pmc Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908 0733, USA
    J Mol Biol 376:607-13. 2008
    ..We suggest that the stapling of subdomain 1 and subdomain 4 of two actin protomers on different strands is a common mechanism for F-actin stabilization utilized by many actin-binding proteins that have no homology...
  24. pmc Structural polymorphism in F-actin
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA
    Nat Struct Mol Biol 17:1318-23. 2010
    ..Because F-actin is structurally polymorphic, it cannot be described using only one atomic model and must be understood as an ensemble of different states...
  25. doi request reprint Divergence of quaternary structures among bacterial flagellar filaments
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia, Charlottesville, VA 22908 0733, USA
    Science 320:382-5. 2008
    ..The driving force in the change of quaternary structure between Salmonella and Campylobacter may have been the evasion of TLR5...
  26. ncbi request reprint ATP-mediated conformational changes in the RecA filament
    Margaret S VanLoock
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, Charlottesville, VA 22908, USA
    Structure 11:187-96. 2003
    ..This simple rotation can explain the large cooperativity in ATP hydrolysis observed for RecA-DNA filaments...
  27. ncbi request reprint The structure of a filamentous bacteriophage
    Ying A Wang
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908 0733, USA
    J Mol Biol 361:209-15. 2006
    ..These results show that new computational approaches to helical reconstruction can greatly extend the ability to visualize heterogeneous protein polymers at a reasonably high resolution...
  28. pmc High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    Proc Natl Acad Sci U S A 105:1494-8. 2008
    ....
  29. ncbi request reprint A new internal mode in F-actin helps explain the remarkable evolutionary conservation of actin's sequence and structure
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Curr Biol 12:570-5. 2002
    ..Remarkably, we find that in the tilted state actin subunits make new contacts with neighboring subunits that also involve these inserts, suggesting a key role for these elements in F-actin polymorphism...
  30. ncbi request reprint The Arg non-receptor tyrosine kinase modifies F-actin structure
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 346:565-75. 2005
    ..The conformational changes in the filament introduced by Arg can explain the cooperative binding of Arg to F-actin and might prevent other actin binding proteins from binding to actin filaments...
  31. pmc Cleavage of bacteriophage lambda cI repressor involves the RecA C-terminal domain
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Mol Biol 385:779-87. 2009
    ..Under conditions where the repressor cleavage rates are the highest, cI is coordinated within the groove by contacts with RecA's CTD. These observations provide a framework for understanding previous genetic and biochemical observations...
  32. ncbi request reprint SV40 large T antigen hexamer structure: domain organization and DNA-induced conformational changes
    Margaret S VanLoock
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, Box 800733, Charlottesville, VA 22908 0733, USA
    Curr Biol 12:472-6. 2002
    ....
  33. pmc ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Jordan Hall, Charlottesville, VA 22908 0733, USA
    J Cell Biol 163:1057-66. 2003
    ..We suggest that AC proteins use an intrinsic mechanism of F-actin's internal instability to depolymerize/sever actin filaments in the cell...
  34. pmc Opening of tandem calponin homology domains regulates their affinity for F-actin
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia, USA
    Nat Struct Mol Biol 17:614-6. 2010
    ....
  35. ncbi request reprint Xin-repeats and nebulin-like repeats bind to F-actin in a similar manner
    Olga Cherepanova
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 356:714-23. 2006
    ..Taken together with published data about nebulin, tropomyosin and ADF/cofilin, our results suggest that the ability to bind in multiple modes to the actin protomer is a general property of many actin-binding proteins...
  36. pmc The utrophin actin-binding domain binds F-actin in two different modes: implications for the spectrin superfamily of proteins
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Cell Biol 157:243-51. 2002
    ..The cell may use the modular CH domains found in the spectrin superfamily of actin-binding proteins to bind actin in manifold ways, allowing for complexity to arise from the interactions of a relatively few simple modules with actin...
  37. ncbi request reprint The stalk region of dynamin drives the constriction of dynamin tubes
    Yen Ju Chen
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA
    Nat Struct Mol Biol 11:574-5. 2004
    ..We solved the nonconstricted state by a single-particle approach and show that the stalk region of dynamin undergoes a large conformational change that drives tube constriction...
  38. ncbi request reprint Structural polymorphism in bacterial EspA filaments revealed by cryo-EM and an improved approach to helical reconstruction
    Ying A Wang
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia, Charlottesville, VA 22908, USA
    Structure 14:1189-96. 2006
    ..Outside of a crystal, where there is nothing to maintain long-range order, the structural polymorphism in helical polymers may be much greater than has been assumed...
  39. pmc BRCA2 BRC motifs bind RAD51-DNA filaments
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    Proc Natl Acad Sci U S A 102:8537-42. 2005
    ....
  40. pmc Helical filaments of human Dmc1 protein on single-stranded DNA: a cautionary tale
    Xiong Yu
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Mol Biol 401:544-51. 2010
    ..While three-dimensional reconstruction of helical filaments from EM images is a powerful tool, the intrinsic ambiguities that may be present with limited resolution are not sufficiently appreciated...
  41. pmc The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings
    Xiong Yu
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    EMBO Rep 3:792-7. 2002
    ..While the structure of the ring is very similar to that of hexameric replicative helicases such as bacteriophage T7 gp4, our results show that such ring structures may not be constrained to have only six subunits...
  42. pmc The structure of the Salmonella typhimurium type III secretion system needle shows divergence from the flagellar system
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, VA 22908 0733, USA
    J Mol Biol 396:1392-7. 2010
    ..In contrast to the Sa. typhimurium flagellar filament, which shows a nearly crystalline order, the Sa. typhimurium needle has a highly variable symmetry, which could be used to transmit information about host cell contact...
  43. pmc The structure of F-pili
    Ying A Wang
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Mol Biol 385:22-9. 2009
    ..This coexistence of two very different symmetries is similar to what has recently been reported for an archaeal Methanococcus maripaludis pili filament and an archaeal Sulfolobus shibatae flagellar filament...
  44. ncbi request reprint Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments
    Margaret S VanLoock
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 333:345-54. 2003
    ..Since RecX binds from the C-terminal domain of one subunit to the nucleotide-binding core of another subunit, a stabilization of RecA's C-terminal domain by RecX can likely explain the inhibition of RecA's ATPase activity by RecX...
  45. ncbi request reprint Acrosomal actin: twists and turns of a versatile filament
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Box 800733, Charlottesville, Virginia 22908 0733, USA
    Curr Biol 14:R959-61. 2004
    ..The twist and tilt of the actin subunits show very large deviations from ideal F-actin, providing clues about actin dynamics...
  46. ncbi request reprint Structural polymorphism of Methanothermobacter thermautotrophicus MCM
    Yen Ju Chen
    Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Mol Biol 346:389-94. 2005
    ..This offers new insight into the conformational dynamics of MCM and the phosphorylation-bypass phenotype in yeast...
  47. pmc The structure of an archaeal pilus
    Ying A Wang
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, VA 22908 0733, USA
    J Mol Biol 381:456-66. 2008
    ..This result has many implications for understanding the evolutionary divergence of bacteria and archaea...
  48. ncbi request reprint Flexibility of the rings: structural asymmetry in the DnaB hexameric helicase
    Shixin Yang
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Jordan Hall, Box 800773, Charlottesville 22908 0733, USA
    J Mol Biol 321:839-49. 2002
    ....
  49. ncbi request reprint The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Box 800733, Charlottesville, Virginia 22908, USA
    Structure 14:983-92. 2006
    ..These results show that the N-terminal domains play a regulatory role in filament activation and highlight the modular architecture of the recombination proteins...
  50. ncbi request reprint Cell walls, cell shape, and bacterial actin homologs
    Edward H Egelman
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Dev Cell 5:4-5. 2003
    ..The synthesis of the peptidoglycan layer, one of the key determinants of cell shape in B. subtilis, has been shown by Daniel and Errington to occur in a helical pattern. This pattern is generated by the actin homolog Mbl...
  51. pmc Assembly of Weibel-Palade body-like tubules from N-terminal domains of von Willebrand factor
    Ren Huai Huang
    Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 105:482-7. 2008
    ..2 units per turn. The symmetry and location of interdomain contacts suggest that decreasing pH along the secretory pathway coordinates the disulfide-linked assembly of VWF multimers with their tubular packaging...
  52. ncbi request reprint Actin hydrophobic loop 262-274 and filament nucleation and elongation
    Alexander Shvetsov
    Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    J Mol Biol 375:793-801. 2008
    ..Our findings show an importance of the hydrophobic loop conformational dynamics in both actin nucleation and elongation and reveal that the inhibition of these two steps in the cross-linked actin can be relieved by appropriate factors...
  53. ncbi request reprint Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms
    Mirjana Lilic
    Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA
    Science 301:1918-21. 2003
    ..Deletion analysis of the tethering arms provides strong support for this model...
  54. ncbi request reprint Refining the structure of the Halobacterium salinarum flagellar filament using the iterative helical real space reconstruction method: insights into polymorphism
    Shlomo Trachtenberg
    Department of Membrane and Ultrastructural Research, The Hebrew University of Jerusalem Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Mol Biol 346:665-76. 2005
    ..Single alpha-helices can be fit into the reconstruction, supporting the proposed similarity of the structure to that of type IV bacterial pili...
  55. ncbi request reprint A DNA pairing-enhanced conformation of bacterial RecA proteins
    Nami Haruta
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 278:52710-23. 2003
    ..3) RecA filaments bound to dsDNA (P state) have directly observable structural changes relative to RecA filaments bound to ssDNA (A state), involving the C-terminal domain...
  56. ncbi request reprint Mapping the interaction of cofilin with subdomain 2 on actin
    Sabrina A Benchaar
    Department of Chemistry and Biochemistry, Molecular Biology Institute and Department of Biological Chemistry, University of California, Los Angeles, California 90095, USA
    Biochemistry 46:225-33. 2007
    ..Overall, these results demonstrate that the region around Gln41 (subdomain 2) is involved in a weak binding of cofilin to G-actin...
  57. ncbi request reprint More insights into structural plasticity of actin binding proteins
    Edward H Egelman
    Structure 12:909-10. 2004
  58. ncbi request reprint Homologous-pairing activity of the Bacillus subtilis bacteriophage SPP1 replication protein G35P
    Silvia Ayora
    Departmento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus Universidad Autonoma de Madrid, Madrid 28049, Spain
    J Biol Chem 277:35969-79. 2002
    ..The loading of G40P at a d-loop (ori DNA or at any stalled replication fork) by G35P could lead to replication fork reactivation...
  59. doi request reprint Functional and structural basis for a bacteriophage homolog of human RAD52
    Mickael Ploquin
    Genome Stability Laboratory, Laval University Cancer Research Center, Hotel Dieu de Quebec, 9 McMahon, Quebec City G1R 2J6, Canada
    Curr Biol 18:1142-6. 2008
    ..For the first time, we propose a viral homolog of RAD52 at the amino acid, phylogenic, functional, and structural levels...
  60. ncbi request reprint Atomic model of a myosin filament in the relaxed state
    John L Woodhead
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Nature 436:1195-9. 2005
    ....
  61. pmc A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments
    Sean D Sheridan
    Committee on Genetics, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 36:4057-66. 2008
    ....
  62. ncbi request reprint Helical structure of the needle of the type III secretion system of Shigella flexneri
    Frank S Cordes
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 278:17103-7. 2003
    ..6 subunits/turn, 24-A helical pitch). This common architecture implies that there will be further mechanistic analogies in the functioning of these two bacterial systems...
  63. pmc Structural basis of membrane invagination by F-BAR domains
    Adam Frost
    Departments of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510, USA
    Cell 132:807-17. 2008
    ..The geometric constraints and sequential assembly of the helical lattice explain how F-BAR and classical BAR domains segregate into distinct microdomains, and provide insight into the spatial regulation of membrane invagination...
  64. pmc How does ATP hydrolysis control actin's associations?
    Elena P Sablin
    Department of Biochemistry Biophysics, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 99:10945-7. 2002
  65. pmc Structure and function of Hib pili from Haemophilus influenzae type b
    Xiang Qi Mu
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118 2526, USA
    J Bacteriol 184:4868-74. 2002
    ..This capacity to resist unwinding may be important for continued adherence of H. influenzae type b to the nasopharynx, where the three-stranded Hib pilus filaments provide a robust tether to withstand coughs and sneezes...
  66. ncbi request reprint Actin structure and function: what we still do not understand
    Emil Reisler
    Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 282:36133-7. 2007
  67. ncbi request reprint Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2
    Fumiko Esashi
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Nat Struct Mol Biol 14:468-74. 2007
    ..We suggest that interactions of the BRCA2 C-terminal region with RAD51 may facilitate efficient nucleation of RAD51 multimers on DNA and thereby stimulate recombination-mediated repair...
  68. ncbi request reprint RecA assembly, one molecule at a time
    Edward H Egelman
    Structure 14:1600-2. 2006