Rg Eckenhoff

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint The common inhalational anesthetic isoflurane induces apoptosis via activation of inositol 1,4,5-trisphosphate receptors
    Huafeng Wei
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Anesthesiology 108:251-60. 2008
  2. pmc m-Azipropofol (AziPm) a photoactive analogue of the intravenous general anesthetic propofol
    Michael A Hall
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    J Med Chem 53:5667-75. 2010
  3. pmc A high-throughput approach for identification of novel general anesthetics
    Wendy A Lea
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 4:e7150. 2009
  4. pmc Differential general anesthetic effects on microglial cytokine expression
    Xuefei Ye
    Department of Anesthesiology and Critical Care, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e52887. 2013
  5. pmc Anesthesia, surgery, illness and Alzheimer's disease
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA Electronic address
    Prog Neuropsychopharmacol Biol Psychiatry 47:162-6. 2013
  6. pmc Ferritin couples iron and fatty acid metabolism
    Weiming Bu
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    FASEB J 26:2394-400. 2012
  7. doi request reprint Anesthetic mechanisms: worms light the way
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Biol 21:R985-6. 2011
  8. doi request reprint Inhaled anesthetics elicit region-specific changes in protein expression in mammalian brain
    Jonathan Z Pan
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proteomics 8:2983-92. 2008
  9. ncbi request reprint Why can all of biology be anesthetized?
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Anesth Analg 107:859-61. 2008
  10. doi request reprint From anesthetic mechanisms research to drug discovery
    Rg Eckenhoff
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Clin Pharmacol Ther 84:144-8. 2008

Research Grants

  1. Anesthetics and Alzheimer's Disease
    Roderic G Eckenhoff; Fiscal Year: 2010
  2. Interaction oh inhalational anesthetics with macromolecules
    Roderic Eckenhoff; Fiscal Year: 2007
  3. INHALATIONAL ANESTHETIC BINDING STUDIES
    Roderic Eckenhoff; Fiscal Year: 2007
  4. Interaction of amyloid beta and inhaled anesthetics
    Roderic Eckenhoff; Fiscal Year: 2006
  5. INHALATIONAL ANESTHETIC BINDING STUDIES
    Roderic Eckenhoff; Fiscal Year: 2003

Collaborators

Detail Information

Publications47

  1. doi request reprint The common inhalational anesthetic isoflurane induces apoptosis via activation of inositol 1,4,5-trisphosphate receptors
    Huafeng Wei
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Anesthesiology 108:251-60. 2008
    ..The authors hypothesized that isoflurane activates inositol 1,4,5-trisphosphate (IP3) receptors on the endoplasmic reticulum (ER) membrane, causing excessive calcium release, triggering apoptosis...
  2. pmc m-Azipropofol (AziPm) a photoactive analogue of the intravenous general anesthetic propofol
    Michael A Hall
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    J Med Chem 53:5667-75. 2010
    ..Using either mass spectrometry or radiolabeling, this reagent may be used to reveal sites and targets that underlie the mechanism of both the desirable and undesirable actions of this important clinical compound...
  3. pmc A high-throughput approach for identification of novel general anesthetics
    Wendy A Lea
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 4:e7150. 2009
    ..These hits were validated using isothermal titration calorimetry. The success of this initial screen and computational triage provides feasibility to undergo a large scale campaign to discover novel general anesthetics...
  4. pmc Differential general anesthetic effects on microglial cytokine expression
    Xuefei Ye
    Department of Anesthesiology and Critical Care, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e52887. 2013
    ..These data suggest that propofol may be a preferred anesthetic when it is desirable to minimize neuroinflammation...
  5. pmc Anesthesia, surgery, illness and Alzheimer's disease
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA Electronic address
    Prog Neuropsychopharmacol Biol Psychiatry 47:162-6. 2013
    ..It is highly likely that inflammatory cascades are at the heart of this intersection, and if confirmed, this suggests a therapeutic strategy to mitigate enhanced neuropathology in vulnerable surgical patients. ..
  6. pmc Ferritin couples iron and fatty acid metabolism
    Weiming Bu
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    FASEB J 26:2394-400. 2012
    ....
  7. doi request reprint Anesthetic mechanisms: worms light the way
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Biol 21:R985-6. 2011
    ....
  8. doi request reprint Inhaled anesthetics elicit region-specific changes in protein expression in mammalian brain
    Jonathan Z Pan
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proteomics 8:2983-92. 2008
    ..A pronounced degree of regional selectivity was noted, indicating an under appreciated degree of specificity for these promiscuous drugs...
  9. ncbi request reprint Why can all of biology be anesthetized?
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Anesth Analg 107:859-61. 2008
  10. doi request reprint From anesthetic mechanisms research to drug discovery
    Rg Eckenhoff
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Clin Pharmacol Ther 84:144-8. 2008
    ..Worse, there is growing concern that the anesthetic drugs in current use, especially the inhaled anesthetics, have durable adverse effects on cognition...
  11. ncbi request reprint Inhaled anesthetic enhancement of amyloid-beta oligomerization and cytotoxicity
    Roderic G Eckenhoff
    Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania 19104 4283, USA
    Anesthesiology 101:703-9. 2004
    ..The authors hypothesize that inhaled anesthetics contribute to these effects through a durable enhancement of peptide oligomerization...
  12. doi request reprint Inhalational anesthetic photolabeling
    Roderic G Eckenhoff
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Methods Mol Biol 617:437-43. 2010
    ..There are, however, few groups doing this work, so this article is an attempt to demystify the method. We will discuss the theory, method, and limitations of this useful experimental approach...
  13. ncbi request reprint Halogenated diazirines as photolabel mimics of the inhaled haloalkane anesthetics
    Roderic G Eckenhoff
    Department of Anesthesia, University of Pennsylvania Health System, 3400 Spruce Street, Philadelphia, Pennsylvania 19104 4283, USA
    J Med Chem 45:1879-86. 2002
    ..When tagged with radioactivity, this compound should serve as a reasonable probe of haloalkane anesthetic binding targets and sites...
  14. ncbi request reprint Promiscuous ligands and attractive cavities: how do the inhaled anesthetics work?
    R G Eckenhoff
    Department of Anesthesiology, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104 4283, USA
    Mol Interv 1:258-68. 2001
    ..Hence, it is essential that we develop an understanding of their molecular pharmacology so that safer alternatives can be developed...
  15. pmc Identification of a fluorescent general anesthetic, 1-aminoanthracene
    Christopher A Butts
    Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 106:6501-6. 2009
    ..Thus, 1-AMA provides an unprecedented opportunity for studying general anesthetic distribution in vivo at the cellular and subcellular levels...
  16. ncbi request reprint The role of electrostatic interactions in human serum albumin binding and stabilization by halothane
    Renyu Liu
    Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania 19104 4283, USA
    J Biol Chem 277:36373-9. 2002
    ....
  17. pmc Comparative binding character of two general anaesthetics for sites on human serum albumin
    Renyu Liu
    Department of Anesthesia, University of Pennsylvania Medical Center, 335 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104 6112, USA
    Biochem J 380:147-52. 2004
    ....
  18. ncbi request reprint Volatile anesthetic modulation of oligomerization equilibria in a hexameric model peptide
    Giovanna Ghirlanda
    Department of Biophysics and Biochemistry, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    FEBS Lett 578:140-4. 2004
    ..Inhaled anesthetic modulation of oligomerization interactions is a novel and potentially generalizable biophysical basis for some anesthetic actions...
  19. ncbi request reprint Structural basis for high-affinity volatile anesthetic binding in a natural 4-helix bundle protein
    Renyu Liu
    Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    FASEB J 19:567-76. 2005
    ....
  20. pmc Multiple binding sites for the general anesthetic isoflurane identified in the nicotinic acetylcholine receptor transmembrane domain
    Grace Brannigan
    Institute for Computational and Molecular Science, Temple University, Philadelphia, PA 19122, USA
    Proc Natl Acad Sci U S A 107:14122-7. 2010
    ..Interpreted in the context of existing structural and physiological data, the present MD results support a multisite model for the mechanism of receptor-channel modulation by anesthetics...
  21. doi request reprint Inhaled anesthetic potency in aged Alzheimer mice
    Shannon L Bianchi
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Anesth Analg 110:427-30. 2010
    ..General anesthesia may exacerbate symptoms of and the pathology underlying AD, so minimizing anesthetic exposure may be important. This requires knowledge of whether the continuing AD pathogenesis alters anesthetic potency...
  22. ncbi request reprint Brain and behavior changes in 12-month-old Tg2576 and nontransgenic mice exposed to anesthetics
    Shannon L Bianchi
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, 305 John Morgan, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    Neurobiol Aging 29:1002-10. 2008
    ..These findings indicate that inhaled anesthetics influence cognition and amyloidogenesis, but that the mechanistic relationship remains unclear...
  23. ncbi request reprint Halothane binding proteome in human brain cortex
    Jonathan Z Pan
    Department of Anesthesiology and Critical Care, University of Pennsylvania Health System, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Proteome Res 6:582-92. 2007
    ..This study provides evidence for multiple anesthetic binding targets and suggests potential pathways involved in their actions...
  24. ncbi request reprint Independent cerebral vasoconstrictive effects of hyperoxia and accompanying arterial hypocapnia at 1 ATA
    Thomas F Floyd
    Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104 4283, USA
    J Appl Physiol (1985) 95:2453-61. 2003
    ..Regression analysis confirmed the independent cerebral vasoconstrictive effect of increased arterial Po2. The present results also demonstrate that the magnitude of this effect at 1.0 ATA is greater than previously measured...
  25. ncbi request reprint Binding of the volatile general anesthetics halothane and isoflurane to a mammalian beta-barrel protein
    Jonas S Johansson
    Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104, USA
    FEBS J 272:573-81. 2005
    ..Such studies will provide much needed insight into how volatile anesthetics interact with biological macromolecules...
  26. ncbi request reprint Isoflurane and sevoflurane affect cell survival and BCL-2/BAX ratio differently
    Huafeng Wei
    Department of Anesthesia, 305 John Morgan Building, 3620 Hamilton Walk, University of Pennsylvania Health System, Philadelphia, PA 19104 4283, USA
    Brain Res 1037:139-47. 2005
    ..At equipotent concentrations, isoflurane, but not sevoflurane, induces cytotoxicity in both PC12 cells and primary cortical neurons and decreases the Bcl-2/Bax ratio...
  27. ncbi request reprint Inhalational anesthetic-binding proteins in rat neuronal membranes
    Jin Xi
    Department of Anesthesia, University of Pennsylvania Health System, 305 John Morgan, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Biol Chem 279:19628-33. 2004
    ....
  28. ncbi request reprint Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions
    Jin Xi
    Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    ACS Chem Biol 1:377-84. 2006
    ..H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics...
  29. pmc Partitioning of anesthetics into a lipid bilayer and their interaction with membrane-bound peptide bundles
    Satyavani Vemparala
    Department of Chemistry and Center for Molecular Modeling, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 91:2815-25. 2006
    ..The observed local and global effects of anesthetic binding on protein motions demonstrated in this study may underlie the mechanism of action of anesthetics at a molecular level...
  30. ncbi request reprint G protein-coupled receptors as direct targets of inhaled anesthetics
    Yumiko Ishizawa
    Department of Anesthesia, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 4283, USA
    Mol Pharmacol 61:945-52. 2002
    ..These data suggest that competition with endogenous ligands may be a general mechanism of the action of halothane at this large family of signaling proteins...
  31. ncbi request reprint Low-affinity analytical chromatography for measuring inhaled anesthetic binding to isolated proteins
    Kin Chan
    Department of Anesthesia, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104, USA
    Anal Biochem 301:308-13. 2002
    ..This approach will be useful in directly characterizing equilibrium, solution binding to isolated proteins in preparation for more time-consuming methods with structural resolution...
  32. ncbi request reprint A guest molecule-host cavity fitting algorithm to mine PDB for small molecule targets
    William C Byrem
    Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA, 19104, USA
    Biochim Biophys Acta 1764:1320-4. 2006
    ..Proteins with large numbers of such cavities are more likely to be functionally altered by halothane...
  33. pmc Anesthetic-induced neurodegeneration mediated via inositol 1,4,5-trisphosphate receptors
    Yifan Zhao
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, 305 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    J Pharmacol Exp Ther 333:14-22. 2010
    ....
  34. ncbi request reprint Weak polar interactions confer albumin binding site selectivity for haloether anesthetics
    Renyu Liu
    Department of Anesthesia, University of Pennsylvania Health System, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    Anesthesiology 102:799-805. 2005
    ..In this study, the authors examined binding site selectivity of these anesthetics in human serum albumin (HSA)...
  35. pmc A unitary anesthetic binding site at high resolution
    L Sangeetha Vedula
    Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 284:24176-84. 2009
    ..Finally, apoferritin displays both structural and dynamic responses to anesthetic binding, which may mimic changes elicited by anesthetics in physiologic targets like ion channels...
  36. ncbi request reprint Multiple specific binding targets for inhaled anesthetics in the mammalian brain
    Maryellen Fazen Eckenhoff
    Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Pharmacol Exp Ther 300:172-9. 2002
    ..We conclude that halothane binding motifs are sufficiently degenerate to be found in many proteins, both soluble and membrane-bound...
  37. ncbi request reprint Rat brain DNA transcript profile of halothane and isoflurane exposure
    Jonathan Z Pan
    Department of Anesthesiology and Critical Care, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104, USA
    Pharmacogenet Genomics 16:171-82. 2006
    ..In addition, the data show different gene expression profiles for the two inhaled anesthetics, suggesting unique pharmacological targets and mechanisms in each case...
  38. doi request reprint A presenilin-1 mutation renders neurons vulnerable to isoflurane toxicity
    Ge Liang
    Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104, USA
    Anesth Analg 106:492-500, table of contents. 2008
    ..Sevoflurane and desflurane have less ability to disrupt intracellular calcium homeostasis; and thus we predict they will cause less cytotoxicity...
  39. ncbi request reprint Thermodynamics of xenon binding to cryptophane in water and human plasma
    P Aru Hill
    Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104 6323, USA
    J Am Chem Soc 129:9262-3. 2007
  40. ncbi request reprint Interactions of volatile anesthetics with neurodegenerative-disease-associated proteins
    Anna Carnini
    Department of Anesthesiology and Critical Care, University of Pennsylvania Health Systems, 305 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104, USA
    Anesthesiol Clin 24:381-405. 2006
    ..Many opportunities exist for rapid progress at all levels on defining whether anesthetics do indeed contribute to the pathogenesis of these progressive, debilitating disorders...
  41. doi request reprint Anesthesia and the old brain
    Junxia Tang
    Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Anesth Analg 110:421-6. 2010
    ..In this special article, we summarize the rationale and evidence that the anesthetic per se is a contributor. The evidence at this point is considered suggestive and further research is needed, especially in humans...
  42. ncbi request reprint Identification of nicotinic acetylcholine receptor amino acids photolabeled by the volatile anesthetic halothane
    David C Chiara
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 42:13457-67. 2003
    ..Halothane binding at this site may contribute to the functional inhibition of nAChRs...
  43. ncbi request reprint Anesthetic potency of two novel synthetic polyhydric alkanols longer than the n-alkanol cutoff: evidence for a bilayer-mediated mechanism of anesthesia?
    Justin T Mohr
    Department of Chemistry, Dartmouth College, Hanover, New Hampshire 03755, USA
    J Med Chem 48:4172-6. 2005
    ..These results, which contradict a protein-binding mechanism in which cutoff results from steric hindrance, are consistent with recent predictions of a membrane-mediated mechanism involving the lateral pressure profile...
  44. ncbi request reprint Measurement of resiniferatoxin in serum samples by high-performance liquid chromatography
    Andrew J Mannes
    DASS NIH, Building 49, Room 1A08, 49 Convent Drive Bethesda, MD 20892, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 823:184-8. 2005
    ..Therefore, the procedure described provides a useful assay for determination of serum RTX pharmacokinetic parameters...
  45. ncbi request reprint Does it add up?
    Max B Kelz
    Anesth Analg 107:365-6. 2008
  46. ncbi request reprint Image not living up to goal
    Lee A Fleisher
    Anesthesiology 105:626-7; author reply 628, 629-30. 2006

Research Grants21

  1. Anesthetics and Alzheimer's Disease
    Roderic G Eckenhoff; Fiscal Year: 2010
    ....
  2. Interaction oh inhalational anesthetics with macromolecules
    Roderic Eckenhoff; Fiscal Year: 2007
    ....
  3. INHALATIONAL ANESTHETIC BINDING STUDIES
    Roderic Eckenhoff; Fiscal Year: 2007
    ..abstract_text> ..
  4. Interaction of amyloid beta and inhaled anesthetics
    Roderic Eckenhoff; Fiscal Year: 2006
    ..These studies will provide the feasibility to extend this hypothesis to animal models and ultimately to clinical studies of the interaction between anesthetics and neurodegeneration. ..
  5. INHALATIONAL ANESTHETIC BINDING STUDIES
    Roderic Eckenhoff; Fiscal Year: 2003
    ..Further, improved understanding of the mechanisms of unconsciousness should lead to an improved understanding of consciousness. ..