Research Topics
| R G EckenhoffSummaryAffiliation: University of Pennsylvania Country: USA Publications
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Publications
Differential halothane binding and effects on serum albumin and myoglobinR G Eckenhoff
Department of Anesthesia, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104, USA
Biophys J 75:477-83. 1998..Preferential binding and stabilization of different conformational states may underlie anesthetic-induced protein dysfunction, as well as provide an explanation for heterogeneity of action...
Do specific or nonspecific interactions with proteins underlie inhalational anesthetic action?R G Eckenhoff
Departments of Anesthesia and Physiology, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104 4283, USA
Mol Pharmacol 54:610-5. 1998..These observations significantly increase the likelihood that such interactions can be found and optimized...- Cooperative binding of inhaled anesthetics and ATP to firefly luciferaseR G Eckenhoff
Department of Anesthesia, University of Pennsylvania Medical Center, Philadelphia 19104 4283, USA
Proteins 42:436-41. 2001..Our results demonstrate a cooperative binding equilibrium between native ligands and anesthetics, suggesting that similar interactions could underlie actions at biologically relevant targets... - Inhaled anesthetic binding sites in human serum albuminR G Eckenhoff
Department of Anesthesia, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 4283, USA
J Biol Chem 275:30439-44. 2000..Finally, myristate isosterically competes with anesthetic binding in the domain III cavity and allosterically enhances anesthetic binding in the interdomain cleft... - Steric hindrance is not required for n-alkanol cutoff in soluble proteinsR G Eckenhoff
Department of Anesthesia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
Mol Pharmacol 56:414-8. 1999..Finally, these results render cutoff an untenable approach for mapping binding site sterics in the absence of complementary binding measurements, and a poor discriminator of target relevance to general anesthesia... - Anesthetic stabilization of protein intermediates: myoglobin and halothaneR G Eckenhoff
Department of Anesthesia, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104, USA
Biochemistry 40:10819-24. 2001..Specific binding to less stable intermediates may underlie anesthetic potentiation of protein activity... - A designed four-alpha-helix bundle that binds the volatile general anesthetic halothane with high affinityJ S Johansson
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104, USA
Biophys J 78:982-93. 2000..Finally, preferential stabilization of folded protein conformations may represent a fundamental mechanism of inhaled anesthetic action... - Predictability of weak binding from X-ray crystallography: inhaled anesthetics and myoglobinJ W Tanner
Department of Anesthesia, University of Pennsylvania, Philadelphia 19104, USA
Biochemistry 40:5075-80. 2001..These results suggest a need for solution measurements to complement crystallography if the consequences of weak binding to proteins are to be appreciated... - Volatile anesthetics alter protein stabilityJ W Tanner
Department of Anesthesia, University of Pennsylvania, Philadelphia 19104, USA
Toxicol Lett 100:387-91. 1998..4. A shift of Tm in either direction may model the action of inhaled anesthetics on relevant proteins in the central nervous system... - Halothane, an inhalational anesthetic agent, increases folding stability of serum albuminJ W Tanner
Department of Anesthesia, University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, USA
Biochim Biophys Acta 1430:46-56. 1999..Serum albumin is the first protein that has been shown to be stabilized by an inhalational anesthetic... - Halothane binding to a G protein coupled receptor in retinal membranes by photoaffinity labelingY Ishizawa
Departments of Anesthesia, Physiology, and Biochemistry and Biophysics, University of Pennsylvania Medical Center, Philadelphia 19104 4283, USA
Biochemistry 39:8497-502. 2000..The absence of halothane binding to any of the G protein subunits strongly suggests that the functional effects of halothane on GPCR signaling systems are mediated by direct interactions with receptor proteins... - Gamma-aminobutyric acid enhancement of halothane binding in rat cerebellumM F Eckenhoff
Department of Anesthesia, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104 4283, USA
Neurosci Lett 286:111-4. 2000..Glutamate, however, did not enhance halothane binding in any layer. These data confirm the presence of coupling, and thus suggest a direct interaction of halothane with a GABA binding protein... - Promiscuous ligands and attractive cavities: how do the inhaled anesthetics work?R G Eckenhoff
Department of Anesthesiology, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104 4283, USA
Mol Interv 1:258-68. 2001..Hence, it is essential that we develop an understanding of their molecular pharmacology so that safer alternatives can be developed...