Allison D Ebert
Affiliation: University of Wisconsin
- A tetracycline-regulated adenovirus encoding dominant-negative caspase-9 is regulated in rat brain and protects against neurotoxin-induced cell death in vitro, but not in vivoAllison D Ebert
Children s Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60614, USA
Exp Neurol 191:S80-94. 2005..These results suggest that while inhibiting apoptosis at the level of caspase-9 is protective in vitro, it is not protective against 6-OHDA-induced cell death in vivo...
- Induced pluripotent stem cells from a spinal muscular atrophy patientAllison D Ebert
The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
Nature 457:277-80. 2009..As such, it represents a promising resource to study disease mechanisms, screen new drug compounds and develop new therapies...
- Human neural progenitor cells over-expressing IGF-1 protect dopamine neurons and restore function in a rat model of Parkinson's diseaseAllison D Ebert
The Waisman Center and Department of Anatomy, University of Wisconsin Madison, Madison, WI 53705 2280, USA
Exp Neurol 209:213-23. 2008..This suggests a dual role of IGF-1 to both increase hNPC survival after transplantation and exert trophic effects on degenerating dopamine neurons in this rat model of PD...
- Isolating, expanding, and infecting human and rodent fetal neural progenitor cellsAllison D Ebert
Waisman Center, University of Wisconsin Madison, Madison, Wisconsin, USA
Curr Protoc Stem Cell Biol . 2008..Detailed protocols for cryopreservation, neural differentiation, exogenous gene expression using lentivirus, and transplantation into the rodent nervous system are also described...
- Ex vivo delivery of GDNF maintains motor function and prevents neuronal loss in a transgenic mouse model of Huntington's diseaseAllison D Ebert
Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI 53705, USA
Exp Neurol 224:155-62. 2010..Our data show that mNPCs(GDNF) can survive transplantation, secrete GDNF for several weeks and are able to maintain motor function in this model of HD...
- GDNF-secreting human neural progenitor cells increase tyrosine hydroxylase and VMAT2 expression in MPTP-treated cynomolgus monkeysMarina E Emborg
Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA
Cell Transplant 17:383-95. 2008..6) General blood chemistry and necropsies did not reveal any abnormalities. Therefore, we conclude that hNPCs releasing GDNF may be a possible alternative for intracerebral trophic factor delivery in Parkinson's disease...
- Lesion-induced increase in survival and migration of human neural progenitor cells releasing GDNFSoshana Behrstock
The Waisman Center, University of Wisconsin Madison, Madison, WI 53705 2280, USA
Cell Transplant 17:753-62. 2008..We conclude that the type of brain injury dictates migration and integration of hNPC, which has important consequences when considering transplantation of these cells as a therapy for neurodegenerative diseases...
- Stem cell model of spinal muscular atrophyAllison D Ebert
Wisconsin Institutes for Medical Research, 1111 Highland Ave, Room 5033, Madison, WI 53705, USA
Arch Neurol 67:665-9. 2010..Furthermore, they provide an ideal platform for small-molecule screening and subsequent drug development...
- Lentiviral vector-mediated genetic modification of human neural progenitor cells for ex vivo gene therapyElizabeth E Capowski
Stem Cell Research Program, Waisman Center, University of Wisconsin Madison, 1500 Highland Ave, Madison, WI 53705, USA
J Neurosci Methods 163:338-49. 2007..The methods presented provide a powerful tool for assessing the impact of factors such as promoter systems or different transgenes on the therapeutic utility of these cells...
- Chromosome 7 and 19 trisomy in cultured human neural progenitor cellsDhruv Sareen
Department of Neurology, University of Wisconsin School of Medicine and Public Health, Wisconsin Institutes for Medical Research WIMR, Madison, Wisconsin, USA
PLoS ONE 4:e7630. 2009..Therefore, we investigated frequently occurring chromosomal abnormalities in 21 independent fetal-derived hNPC lines and the possible mechanisms triggering such aberrations...
- Human stem cells and drug screening: opportunities and challengesAllison D Ebert
Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1111 Highland Avenue, Wisconsin 53705, USA
Nat Rev Drug Discov 9:367-72. 2010..This article discusses the opportunities and challenges for the use of stem cells in drug screening with a focus on induced pluripotent stem cells...
- A new tool in the battle against Alzheimer's disease and aging: ex vivo gene therapyAllison D Ebert
The Waisman Center Stem Cell Research Program and Department of Anatomy, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA
Rejuvenation Res 8:131-4. 2005..Despite the need for further testing, their report illustrated a mild but significant therapeutic benefit of NGF for the treatment of AD and provided important data concerning the safety and efficacy of ex vivo gene therapy in humans...
- Progressive degeneration of dopamine neurons in 6-hydroxydopamine rat model of Parkinson's disease does not involve activation of caspase-9 and caspase-3Allison D Ebert
Department of Pediatrics, Neurobiology Program, Children s Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60614 4314, USA
J Neurosci Res 86:317-25. 2008..These data suggest that in the adult rat brain DA neurons whose terminals are challenged with 6-OHDA do not die through a classical caspase-dependent apoptotic mechanism...