KEVIN F DYBVIG

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. pmc A family of phase-variable restriction enzymes with differing specificities generated by high-frequency gene rearrangements
    K Dybvig
    Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, AL 35294 0019, USA
    Proc Natl Acad Sci U S A 95:13923-8. 1998
  2. pmc Fewer essential genes in mycoplasmas than previous studies suggest
    Kevin Dybvig
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
    FEMS Microbiol Lett 311:51-5. 2010
  3. pmc Genome of Mycoplasma arthritidis
    Kevin Dybvig
    Department of Genetics, University of Alabama Birmingham, Birmingham, Alabama 35294 0024, USA
    Infect Immun 76:4000-8. 2008
  4. pmc Evidence for type III restriction and modification systems in Mycoplasma pulmonis
    Kevin Dybvig
    Department of Genetics, 720 S 20th St, KAUL 720, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    J Bacteriol 189:2197-202. 2007
  5. pmc The Vsa shield of Mycoplasma pulmonis is antiphagocytic
    Brandon M Shaw
    Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Infect Immun 80:704-9. 2012
  6. pmc Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis
    K Dybvig
    Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Bacteriol 182:4343-7. 2000
  7. pmc Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis
    James M Daubenspeck
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Microbiol 72:1235-45. 2009
  8. pmc A stochastic mechanism for biofilm formation by Mycoplasma pulmonis
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, Birmingham AL 35294 0004, USA
    J Bacteriol 189:1905-13. 2007
  9. pmc Mycoplasma biofilms ex vivo and in vivo
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    FEMS Microbiol Lett 295:77-81. 2009
  10. pmc Biofilms protect Mycoplasma pulmonis cells from lytic effects of complement and gramicidin
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, KAUL 720, Birmingham, AL 35294 0004, USA
    Infect Immun 75:3696-9. 2007

Research Grants

  1. Tandemly Repetitive Proteins in Mycoplasmas
    Kevin Dybvig; Fiscal Year: 2007
  2. Mechanisms of Mycoplasmal Disease Pathogenesis
    Kevin Dybvig; Fiscal Year: 2009
  3. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2009
  4. ANTIGENIC VARIATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
  5. MECHANISMS OF MYCOPLASMA INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 1999
  6. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2000
  7. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2002
  8. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
  9. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010

Collaborators

Detail Information

Publications22

  1. pmc A family of phase-variable restriction enzymes with differing specificities generated by high-frequency gene rearrangements
    K Dybvig
    Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, AL 35294 0019, USA
    Proc Natl Acad Sci U S A 95:13923-8. 1998
    ..These data cast doubt on the prevailing paradigms that restriction systems are either selfish or function to confer protection from invasion by foreign DNA...
  2. pmc Fewer essential genes in mycoplasmas than previous studies suggest
    Kevin Dybvig
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
    FEMS Microbiol Lett 311:51-5. 2010
    ..This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but essential in Bacillus subtilis...
  3. pmc Genome of Mycoplasma arthritidis
    Kevin Dybvig
    Department of Genetics, University of Alabama Birmingham, Birmingham, Alabama 35294 0024, USA
    Infect Immun 76:4000-8. 2008
    ..Of the 635 predicted coding regions, 218 were disrupted in a library of 1,100 members. Dispensable genes included the gene coding for the MAM superantigen and genes coding for ribosomal proteins S15, S18, and L15...
  4. pmc Evidence for type III restriction and modification systems in Mycoplasma pulmonis
    Kevin Dybvig
    Department of Genetics, 720 S 20th St, KAUL 720, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    J Bacteriol 189:2197-202. 2007
    ..Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly located on a mobile element...
  5. pmc The Vsa shield of Mycoplasma pulmonis is antiphagocytic
    Brandon M Shaw
    Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Infect Immun 80:704-9. 2012
    ..These results may be pertinent to understanding the functions of similar proteins that have extensive repeat regions in other microbes...
  6. pmc Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis
    K Dybvig
    Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Bacteriol 182:4343-7. 2000
    ..Tn4001T may be of general utility for use as a mycoplasma cloning vehicle because tetM functions in all species of Mycoplasma examined thus far...
  7. pmc Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis
    James M Daubenspeck
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Microbiol 72:1235-45. 2009
    ..Confounding the interpretation of these results is the observation that the mutants missing EPS-I had an eightfold overproduction of an apparent second EPS (EPS-II) containing N-acetylglucosamine...
  8. pmc A stochastic mechanism for biofilm formation by Mycoplasma pulmonis
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, Birmingham AL 35294 0004, USA
    J Bacteriol 189:1905-13. 2007
    ..As variation in the number of Vsa tandem repeats occurs by slipped-strand mispairing, the ability of the mycoplasmas to form a biofilm switches stochastically...
  9. pmc Mycoplasma biofilms ex vivo and in vivo
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    FEMS Microbiol Lett 295:77-81. 2009
    ..This tracheal organ-mounting system can be used to study interactions between biofilms formed by respiratory pathogens and the host epithelium and to identify the factors that contribute to biofilm formation in vivo...
  10. pmc Biofilms protect Mycoplasma pulmonis cells from lytic effects of complement and gramicidin
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, KAUL 720, Birmingham, AL 35294 0004, USA
    Infect Immun 75:3696-9. 2007
    ..The resistance appeared to be localized to those mycoplasmas within tower structures of the biofilms. Biofilm formation may be a mechanism that protects mycoplasmas from host immunity...
  11. pmc The Vsa proteins modulate susceptibility of Mycoplasma pulmonis to complement killing, hemadsorption, and adherence to polystyrene
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, 720 South 20th Street, Kaul Room 720, Birmingham, AL 35294, USA
    Infect Immun 71:5733-8. 2003
    ..As both VsaH and VsaA can mediate adherence to plastic, cytadherence, and susceptibility to complement, we propose that Vsa modulates these phenotypes by nonspecific interactions...
  12. pmc Resistance of Mycoplasma pulmonis to complement lysis is dependent on the number of Vsa tandem repeats: shield hypothesis
    Warren L Simmons
    Department of Genetics, University of Alabama at Birmingham, 720 South 20th Street, Kaul Room 720, Birmingham, AL 35294 0024, USA
    Infect Immun 72:6846-51. 2004
    ..pulmonis surface interactions and function to sterically hinder access of complement to the mycoplasma cell membrane while permitting access of smaller molecules...
  13. pmc Large-scale transposon mutagenesis of Mycoplasma pulmonis
    Christopher T French
    Department of Genetics, University of Alabama Birmingham, Birmingham, AL 35294, USA
    Mol Microbiol 69:67-76. 2008
    ..A mutant with a defect in transport of N-acetylglucosamine was identified...
  14. pmc Avoidance of the host immune system through phase variation in Mycoplasma pulmonis
    Amy M Denison
    Department of Genetics, KAUL, Room 720, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    Infect Immun 73:2033-9. 2005
    ..The data strongly indicate that Vsa phase variation is a mechanism for avoidance of the immune system with no obvious contribution to tissue tropism...
  15. pmc The vir gene of bacteriophage MAV1 confers resistance to phage infection on Mycoplasma arthritidis
    Brenda Clapper
    Department of Microbiology, KAUL, Rm 720, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    J Bacteriol 186:5715-20. 2004
    ..Vir had no effect on MAV1 adsorption. We conclude that Vir is not a virulence factor but functions to exclude superinfecting phage, possibly by blocking the injection of phage DNA into the bacterial cytoplasm...
  16. pmc Association of Mycoplasma arthritidis mitogen with lethal toxicity but not with arthritis in mice
    Wenyi Luo
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    Infect Immun 76:4989-98. 2008
    ..The degree of mitogenic activity correlated with lethal toxicity in DBA/2J mice. In contrast, histopathological studies detected no correlation between MAM production and the severity of arthritis induced in DBA/2J and CBA/J mice...
  17. pmc Gene transfer in Mycoplasma pulmonis
    Amy M Teachman
    Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Bacteriol 184:947-51. 2002
    ..Analysis of the progeny indicated that only the transposon and not flanking genomic DNA was transferred to the recipient cell. Gene transfer was DNase resistant and probably the result of conjugation or cell fusion...
  18. pmc Bacteriophage MAV1 is not associated with virulence of Mycoplasma arthritidis
    Brenda Clapper
    Department of Genetics, KAUL, Room 720, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    Infect Immun 72:7322-5. 2004
    ..The virulence of 158 and 158-1 did not increase upon lysogenization. A major antigenic difference between 158 and 158-1 was identified that is unrelated to MAV1 and could account for the difference in virulence...
  19. pmc Trinucleotide GAA repeats dictate pMGA gene expression in Mycoplasma gallisepticum by affecting spacing between flanking regions
    Li Liu
    Department of Genomics and Pathobiology, Volker Hall, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Bacteriol 184:1335-9. 2002
    ..These data indicate that the nucleotides 5" of the GAA repeats as well as the spacing between the GAA repeats and sequences downstream (3") of the repeats are important for pMGA gene expression...
  20. ncbi request reprint Role of bacteriophage MAV1 as a mycoplasmal virulence factor for the development of arthritis in mice and rats
    Anh Hue T Tu
    Department of Genomics and Pathobiology, University of Alabama at Birmingham, 35294 0019, USA
    J Infect Dis 186:432-5. 2002
    ..Specifically, the MAV1 lysogen 158L3-1 was more virulent than the nonlysogen strain 158 in DBA/2NCr, C3H/HeNCr, C3H/HeJ, and C3Smn.CB17-Prkdc(scid)/J mice, as well as in LEW rats...
  21. ncbi request reprint A unique, bifunctional site-specific DNA recombinase from Mycoplasma pulmonis
    Ramakrishnan Sitaraman
    Departments of Genomics and Pathobiology, University of Alabama at Birmingham, AL 35294, USA
    Mol Microbiol 46:1033-40. 2002
    ..Thus, HvsR is a site-specific DNA recombinase with dual substrate specificity...
  22. pmc Identification of an isoschizomer of the HhaI DNA methyltransferase in Mycoplasma arthritidis
    Wenyi Luo
    Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294 0024, USA
    FEMS Microbiol Lett 290:195-8. 2009
    ..Transformation of M. arthritidis was not significantly affected by modification of plasmid at HhaI sites, indicating that the mycoplasma lacks a restriction endonuclease that recognizes GCGC sites...

Research Grants28

  1. Tandemly Repetitive Proteins in Mycoplasmas
    Kevin Dybvig; Fiscal Year: 2007
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
  2. Mechanisms of Mycoplasmal Disease Pathogenesis
    Kevin Dybvig; Fiscal Year: 2009
    ..Mutants that do not survive will be characterized further to determine whether their defect is a failure to grow in vivo, to adhere to the epithelium, or to resist host defenses. ..
  3. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2009
    ..An understanding of the inability of 158-1 to resist host defenses may offer an unique opportunity to explore interactions between the mycoplasma and innate immunity. ..
  4. ANTIGENIC VARIATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ....
  5. MECHANISMS OF MYCOPLASMA INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 1999
    ..arthritidis chromosome and/or specifically mutated to construct strains that will be examined for arthritogenicity in the rat model and used to conclusively identify the MAV1 genes that are involved in the development of arthritis. ..
  6. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2000
    ..The fifth and final aim is to expand our repertoire of subclones containing DNA inversions and other rearrangements in order to more fully characterize the potential for genetic variation in this system. ..
  7. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2002
    ..arthritidis by affecting interactions with host factors such as B and T cells and complement. Thus, the process of dissecting the function of MAV1 in virulence will be begun. ..
  8. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
  9. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..