Research Topics
Species | ROBERT DURONIOSummaryAffiliation: University of North Carolina Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Signaling pathways that control cell proliferationRobert J Duronio
Department of Biology and Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Cold Spring Harb Perspect Biol 5:a008904. 2013..Consequently, both mitogenic and antiproliferative signals exert their effects on cell proliferation through the transcriptional regulation and ubiquitin-dependent degradation of cyclins and CDK inhibitors...
Developing S-phase controlRobert J Duronio
Department of Biology, Department of Genetics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
Genes Dev 26:746-50. 2012....- Mutations of the Drosophila dDP, dE2F, and cyclin E genes reveal distinct roles for the E2F-DP transcription factor and cyclin E during the G1-S transitionR J Duronio
Department of Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA
Mol Cell Biol 18:141-51. 1998.... 
Establishing links between developmental signaling pathways and cell-cycle regulation in DrosophilaR J Duronio
Department of Biology, Campus Box 3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
Curr Opin Genet Dev 9:81-8. 1999..Recent genetic analyses in Drosophila are beginning to reveal the molecular connections between developmental signaling pathways and key regulators of the cell cycle...- Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tailWilliam F Marzluff
Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Nat Rev Genet 9:843-54. 2008..Recent results suggest that the coordinated synthesis of replication-dependent and variant histone mRNAs is achieved by signals that affect formation of the 3' end of the replication-dependent histone mRNAs... - Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequencesWilliam F Marzluff
Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
Curr Opin Cell Biol 14:692-9. 2002..The recent identification of several novel proteins involved in histone gene transcription and pre-mRNA processing has shed light on the variety of mechanisms cells employ to achieve this coupling... - Developmental and cell cycle regulation of the Drosophila histone locus bodyAnne E White
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Mol Biol Cell 18:2491-502. 2007..HLB foci are present in histone deletion embryos, although the MPM-2 foci are smaller, and some Lsm11 foci are not associated with MPM-2 foci, suggesting that the histone locus is important for HLB integrity... - string(cdc25) and cyclin E are required for patterned histone expression at different stages of Drosophila embryonic developmentDavid J Lanzotti
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Dev Biol 274:82-93. 2004..Thus, during the altered cell cycles of early animal development, different cell cycle mechanisms are employed to ensure that the production of histones accompanies DNA synthesis... - Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phaseShusaku T Shibutani
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Dev Cell 15:890-900. 2008..We propose that pRb-independent inhibition of E2F during S phase is an evolutionarily conserved feature of the metazoan cell cycle that is necessary for development... - Drosophila stem-loop binding protein intracellular localization is mediated by phosphorylation and is required for cell cycle-regulated histone mRNA expressionDavid J Lanzotti
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Mol Biol Cell 15:1112-23. 2004..The T230A protein is concentrated in the cytoplasm, suggesting that it is defective in nuclear targeting, and accounting for its failure to function in histone pre-mRNA processing in vivo... - The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are required for histone pre-mRNA processing and play an essential role in developmentAshley C Godfrey
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
RNA 15:1661-72. 2009.... - Endoreplication: polyploidy with purposeHyun O Lee
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Genes Dev 23:2461-77. 2009..Finally, we describe the molecular mechanisms that support the onset and progression of endoreplication... - Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferationHarmony R Salzler
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America
PLoS ONE 4:e8168. 2009..The 3' end stem-loop is necessary for all aspects of histone mRNA metabolism, including replication coupling, but its importance to organism fitness and genome maintenance in vivo have not been characterized... - Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damageJonathan R Hall
Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Biol Chem 283:25356-63. 2008..We conclude that rereplication-associated DNA damage triggers Cdt1 and Cdc6 ubiquitination and destruction, and propose that this pathway represents an evolutionarily conserved mechanism that minimizes the extent of rereplication... - A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processingEric J Wagner
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Cell 28:692-9. 2007.... - Normal regulation of Rbf1/E2f1 target genes in Drosophila type 1 protein phosphatase mutantsLisa M Swanhart
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Dev Dyn 236:2567-77. 2007..We conclude that PP1 is not a major regulator of the Rbf1/E2F1 pathway in Drosophila... - U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesisAshley C Godfrey
Department of Biology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
RNA 12:396-409. 2006..These data suggest that SLBP and U7 snRNP cooperate in the production of histone mRNA in vivo, and that disruption of histone pre-mRNA processing is detrimental to development... - Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesisShusaku Shibutani
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Development 134:467-78. 2007..E2f1 protein reaccumulates in epidermal cells arrested in G1(17), and in these cells the induction of p27(Dap) activates Rbf1 to repress E2f1-target genes to maintain a stable G1 arrest... - Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleasesJeremy M Kupsco
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
RNA 12:2103-17. 2006..Therefore, Snp is a nonessential exonuclease that is not a functional ortholog of either 3'hExo or ERI-1... - A breath of fresh air for cyclin D/Cdk4: triggering growth via HphRobert J Duronio
Department of Biology, Program in Molecular Biology and Biotechnology, and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Dev Cell 6:163-4. 2004..In this issue of Developmental Cell, Frei and Edgar reveal that Cyclin D/Cdk4-stimulated growth requires Hph, a prolyl hydroxylase that is a key component of a cell's ability to respond to hypoxia... - Cancer cell biology: Myc wins the competitionTimothy D Donaldson
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Curr Biol 14:R425-7. 2004.... - Developmental control of growth and cell cycle progression in DrosophilaLisa Swanhart
Department of Biology, University of North Carolina, Chapel Hill, NC, USA
Methods Mol Biol 296:69-94. 2005..This chapter provides an overview of the diverse modes of cell cycle control that are utilized at different stages of Drosophila development... - Targeted disruption of Drosophila Roc1b reveals functional differences in the Roc subunit of Cullin-dependent E3 ubiquitin ligasesTimothy D Donaldson
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Biol Cell 15:4892-903. 2004.... - Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivoDavid J Lanzotti
Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 27599, USA
Mol Cell Biol 22:2267-82. 2002..There is little or no dSLBP protein provided maternally in wild-type embryos, and zygotic expression of dSLBP is immediately required to process newly made histone pre-mRNA... - Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cellsPelin Cayirlioglu
Department of Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Mol Cell Biol 23:2123-34. 2003..In contrast, E2F1 and E2F2 repressors function redundantly for some genes in the embryo. Thus, the relative functional contributions of E2F1 and E2F2 to gene expression and cell cycle control depends on the developmental context... - The contribution of E2F-regulated transcription to Drosophila PCNA gene functionStephen A Thacker
Program in Molecular Biology, University of North Carolina, 27599, Chapel Hill, NC, USA
Curr Biol 13:53-8. 2003..Thus, E2F regulation of PCNA is dispensable for viability, but is nonetheless important for normal Drosophila development... - 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing systemZbigniew Dominski
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA
Mol Cell Biol 22:6648-60. 2002..20 nucleotides downstream from the stem, and an Sm-reactive factor, most likely the Drosophila counterpart of vertebrate U7 snRNP... - Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligaseMaher A Noureddine
Department of Biology, University of North Carolina, Chapel Hill 27599, USA
Dev Cell 2:757-70. 2002..Consequently, the identity of the Roc subunit may contribute to the selection of substrates by metazoan SCF complexes... - WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligaseJian Hu
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Genes Dev 22:866-71. 2008..These results indicate that FBW5-DDB1-CUL4-ROC1 is an E3 ubiquitin ligase regulating TSC2 protein stability and TSC complex turnover... - New insights into cell cycle control from the Drosophila endocycleMary A Lilly
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Oncogene 24:2765-75. 2005..In addition, studies on the endocycle have provided insight into the regulatory principles underlying the once per cell cycle replication of the genome, as well as the relationship between S phase and mitosis...
Research Grants
- NRSA IN GENETICSROBERT DURONIO; Fiscal Year: 2007..Essentially all students publish their dissertation research in peer-reviewed journals. ..
- Developmental Control of the Cell CycleRobert J Duronio; Fiscal Year: 2010..A detailed molecular description of cell cycle events during animal development is critical for our understanding of cell proliferation control, and how such control goes awry in diseases like cancer. ..
- GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENTROBERT DURONIO; Fiscal Year: 2007....
- GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENTROBERT DURONIO; Fiscal Year: 2003....
- Developmental Control of the Cell CycleRobert J Duronio; Fiscal Year: 2010..In this proposal we focus on gene expression mechanisms that control the G1-S transition because this is when most cells decide whether to enter or to exit the cell cycle. ..