ROBERT DURONIO

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation
    Harmony R Salzler
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 4:e8168. 2009
  2. pmc S phase-coupled E2f1 destruction ensures homeostasis in proliferating tissues
    Jean M Davidson
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Genet 8:e1002831. 2012
  3. pmc Drosophila histone locus bodies form by hierarchical recruitment of components
    Anne E White
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 193:677-94. 2011
  4. pmc Cell type-dependent requirement for PIP box-regulated Cdt1 destruction during S phase
    Hyun O Lee
    Curriculum in Genetics and Molecular Biology, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 21:3639-53. 2010
  5. pmc Identifying determinants of cullin binding specificity among the three functionally different Drosophila melanogaster Roc proteins via domain swapping
    Patrick J Reynolds
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 3:e2918. 2008
  6. pmc Signaling pathways that control cell proliferation
    Robert J Duronio
    Department of Biology and Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Cold Spring Harb Perspect Biol 5:a008904. 2013
  7. pmc Developing S-phase control
    Robert J Duronio
    Department of Biology, Department of Genetics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Genes Dev 26:746-50. 2012
  8. pmc Mutations of the Drosophila dDP, dE2F, and cyclin E genes reveal distinct roles for the E2F-DP transcription factor and cyclin E during the G1-S transition
    R J Duronio
    Department of Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA
    Mol Cell Biol 18:141-51. 1998
  9. ncbi request reprint Establishing links between developmental signaling pathways and cell-cycle regulation in Drosophila
    R J Duronio
    Department of Biology, Campus Box 3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Curr Opin Genet Dev 9:81-8. 1999
  10. pmc Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tail
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Genet 9:843-54. 2008

Research Grants

  1. NRSA IN GENETICS
    ROBERT DURONIO; Fiscal Year: 2007
  2. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 1999
  3. Developmental Control of the Cell Cycle
    Robert J Duronio; Fiscal Year: 2010
  4. Developmental Control of the Cell Cycle
    ROBERT DURONIO; Fiscal Year: 2009
  5. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2007
  6. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2006
  7. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2005
  8. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2004
  9. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2003
  10. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2002

Collaborators

  • WILLIAM F contact MARZLUFF
  • Jean M Davidson
  • Yue Xiong
  • Zbigniew Dominski
  • B R Calvi
  • JEANETTE COOK
  • Xiao Yang
  • Mary A Lilly
  • Roopa Thapar
  • BRUCE EDGAR
  • Christoph H Borchers
  • Hyun O Lee
  • Anne E White
  • Ashley C Godfrey
  • David J Lanzotti
  • Jeremy M Kupsco
  • Timothy D Donaldson
  • Harmony R Salzler
  • Brandon D Burch
  • Lisa M Swanhart
  • Patrick J Reynolds
  • Maher A Noureddine
  • Stephen A Thacker
  • Shusaku T Shibutani
  • Jonathan R Hall
  • Jian Hu
  • Shusaku Shibutani
  • Eric J Wagner
  • Lisa Swanhart
  • Pelin Cayirlioglu
  • Pamela Y Gasdaska
  • Sima J Zacharek
  • Deirdre C Tatomer
  • Nathan D Montgomery
  • Elizabeth S Dorn
  • Hyun Lee
  • Yizhou Joseph He
  • Sima Zacharek
  • Greg C Rogers
  • Tânia Reis
  • Stuart Shumway
  • Jeffrey R Simms
  • Brandon D Bunker
  • William J Turbyfill
  • Aida Flor A de la Cruz
  • Vuong Tran
  • Akeisha N Sanders
  • Michelle E Leslie
  • Ming Jing Wu
  • Ryan M Zimmerman
  • Jeremy Kupsco
  • William Bradford
  • William O Ward
  • S Catherine Silver Key
  • Peter C Bonnette
  • Handan Kaygun

Detail Information

Publications34

  1. pmc Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation
    Harmony R Salzler
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 4:e8168. 2009
    ..The 3' end stem-loop is necessary for all aspects of histone mRNA metabolism, including replication coupling, but its importance to organism fitness and genome maintenance in vivo have not been characterized...
  2. pmc S phase-coupled E2f1 destruction ensures homeostasis in proliferating tissues
    Jean M Davidson
    Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Genet 8:e1002831. 2012
    ..We propose that inappropriate accumulation of E2f1 protein during S phase triggers the elimination of potentially hyperplastic cells via apoptosis in order to ensure normal development of rapidly proliferating tissues...
  3. pmc Drosophila histone locus bodies form by hierarchical recruitment of components
    Anne E White
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 193:677-94. 2011
    ....
  4. pmc Cell type-dependent requirement for PIP box-regulated Cdt1 destruction during S phase
    Hyun O Lee
    Curriculum in Genetics and Molecular Biology, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, and Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 21:3639-53. 2010
    ..These data indicate that PIP box-mediated destruction of Dup is necessary for the cell division cycle and suggest that Geminin inhibition can restrain Dup(ΔPIP) activity in some endocycling cell types...
  5. pmc Identifying determinants of cullin binding specificity among the three functionally different Drosophila melanogaster Roc proteins via domain swapping
    Patrick J Reynolds
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 3:e2918. 2008
    ..Our previous genetic data analyzing Roc1a and Roc1b mutants suggested that Roc proteins are functionally distinct, but the molecular basis for this distinction is not known...
  6. pmc Signaling pathways that control cell proliferation
    Robert J Duronio
    Department of Biology and Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Cold Spring Harb Perspect Biol 5:a008904. 2013
    ..Consequently, both mitogenic and antiproliferative signals exert their effects on cell proliferation through the transcriptional regulation and ubiquitin-dependent degradation of cyclins and CDK inhibitors...
  7. pmc Developing S-phase control
    Robert J Duronio
    Department of Biology, Department of Genetics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Genes Dev 26:746-50. 2012
    ....
  8. pmc Mutations of the Drosophila dDP, dE2F, and cyclin E genes reveal distinct roles for the E2F-DP transcription factor and cyclin E during the G1-S transition
    R J Duronio
    Department of Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA
    Mol Cell Biol 18:141-51. 1998
    ....
  9. ncbi request reprint Establishing links between developmental signaling pathways and cell-cycle regulation in Drosophila
    R J Duronio
    Department of Biology, Campus Box 3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Curr Opin Genet Dev 9:81-8. 1999
    ..Recent genetic analyses in Drosophila are beginning to reveal the molecular connections between developmental signaling pathways and key regulators of the cell cycle...
  10. pmc Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tail
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Genet 9:843-54. 2008
    ..Recent results suggest that the coordinated synthesis of replication-dependent and variant histone mRNAs is achieved by signals that affect formation of the 3' end of the replication-dependent histone mRNAs...
  11. ncbi request reprint Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequences
    William F Marzluff
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Curr Opin Cell Biol 14:692-9. 2002
    ..The recent identification of several novel proteins involved in histone gene transcription and pre-mRNA processing has shed light on the variety of mechanisms cells employ to achieve this coupling...
  12. pmc Drosophila stem-loop binding protein intracellular localization is mediated by phosphorylation and is required for cell cycle-regulated histone mRNA expression
    David J Lanzotti
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 15:1112-23. 2004
    ..The T230A protein is concentrated in the cytoplasm, suggesting that it is defective in nuclear targeting, and accounting for its failure to function in histone pre-mRNA processing in vivo...
  13. ncbi request reprint string(cdc25) and cyclin E are required for patterned histone expression at different stages of Drosophila embryonic development
    David J Lanzotti
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Biol 274:82-93. 2004
    ..Thus, during the altered cell cycles of early animal development, different cell cycle mechanisms are employed to ensure that the production of histones accompanies DNA synthesis...
  14. pmc Developmental and cell cycle regulation of the Drosophila histone locus body
    Anne E White
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 18:2491-502. 2007
    ..HLB foci are present in histone deletion embryos, although the MPM-2 foci are smaller, and some Lsm11 foci are not associated with MPM-2 foci, suggesting that the histone locus is important for HLB integrity...
  15. pmc Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase
    Shusaku T Shibutani
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Cell 15:890-900. 2008
    ..We propose that pRb-independent inhibition of E2F during S phase is an evolutionarily conserved feature of the metazoan cell cycle that is necessary for development...
  16. ncbi request reprint A breath of fresh air for cyclin D/Cdk4: triggering growth via Hph
    Robert J Duronio
    Department of Biology, Program in Molecular Biology and Biotechnology, and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Dev Cell 6:163-4. 2004
    ..In this issue of Developmental Cell, Frei and Edgar reveal that Cyclin D/Cdk4-stimulated growth requires Hph, a prolyl hydroxylase that is a key component of a cell's ability to respond to hypoxia...
  17. pmc Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damage
    Jonathan R Hall
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Biol Chem 283:25356-63. 2008
    ..We conclude that rereplication-associated DNA damage triggers Cdt1 and Cdc6 ubiquitination and destruction, and propose that this pathway represents an evolutionarily conserved mechanism that minimizes the extent of rereplication...
  18. pmc The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are required for histone pre-mRNA processing and play an essential role in development
    Ashley C Godfrey
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    RNA 15:1661-72. 2009
    ....
  19. pmc Endoreplication: polyploidy with purpose
    Hyun O Lee
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genes Dev 23:2461-77. 2009
    ..Finally, we describe the molecular mechanisms that support the onset and progression of endoreplication...
  20. ncbi request reprint Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesis
    Shusaku Shibutani
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Development 134:467-78. 2007
    ..E2f1 protein reaccumulates in epidermal cells arrested in G1(17), and in these cells the induction of p27(Dap) activates Rbf1 to repress E2f1-target genes to maintain a stable G1 arrest...
  21. ncbi request reprint A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing
    Eric J Wagner
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 28:692-9. 2007
    ....
  22. ncbi request reprint Normal regulation of Rbf1/E2f1 target genes in Drosophila type 1 protein phosphatase mutants
    Lisa M Swanhart
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Dev Dyn 236:2567-77. 2007
    ..We conclude that PP1 is not a major regulator of the Rbf1/E2F1 pathway in Drosophila...
  23. ncbi request reprint Cancer cell biology: Myc wins the competition
    Timothy D Donaldson
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Curr Biol 14:R425-7. 2004
    ....
  24. ncbi request reprint Developmental control of growth and cell cycle progression in Drosophila
    Lisa Swanhart
    Department of Biology, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Biol 296:69-94. 2005
    ..This chapter provides an overview of the diverse modes of cell cycle control that are utilized at different stages of Drosophila development...
  25. pmc U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis
    Ashley C Godfrey
    Department of Biology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    RNA 12:396-409. 2006
    ..These data suggest that SLBP and U7 snRNP cooperate in the production of histone mRNA in vivo, and that disruption of histone pre-mRNA processing is detrimental to development...
  26. pmc Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases
    Jeremy M Kupsco
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    RNA 12:2103-17. 2006
    ..Therefore, Snp is a nonessential exonuclease that is not a functional ortholog of either 3'hExo or ERI-1...
  27. ncbi request reprint The contribution of E2F-regulated transcription to Drosophila PCNA gene function
    Stephen A Thacker
    Program in Molecular Biology, University of North Carolina, 27599, Chapel Hill, NC, USA
    Curr Biol 13:53-8. 2003
    ..Thus, E2F regulation of PCNA is dispensable for viability, but is nonetheless important for normal Drosophila development...
  28. pmc Targeted disruption of Drosophila Roc1b reveals functional differences in the Roc subunit of Cullin-dependent E3 ubiquitin ligases
    Timothy D Donaldson
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 15:4892-903. 2004
    ....
  29. pmc 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:6648-60. 2002
    ..20 nucleotides downstream from the stem, and an Sm-reactive factor, most likely the Drosophila counterpart of vertebrate U7 snRNP...
  30. ncbi request reprint Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase
    Maher A Noureddine
    Department of Biology, University of North Carolina, Chapel Hill 27599, USA
    Dev Cell 2:757-70. 2002
    ..Consequently, the identity of the Roc subunit may contribute to the selection of substrates by metazoan SCF complexes...
  31. pmc Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivo
    David J Lanzotti
    Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell Biol 22:2267-82. 2002
    ..There is little or no dSLBP protein provided maternally in wild-type embryos, and zygotic expression of dSLBP is immediately required to process newly made histone pre-mRNA...
  32. pmc Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells
    Pelin Cayirlioglu
    Department of Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 23:2123-34. 2003
    ..In contrast, E2F1 and E2F2 repressors function redundantly for some genes in the embryo. Thus, the relative functional contributions of E2F1 and E2F2 to gene expression and cell cycle control depends on the developmental context...
  33. ncbi request reprint New insights into cell cycle control from the Drosophila endocycle
    Mary A Lilly
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Oncogene 24:2765-75. 2005
    ..In addition, studies on the endocycle have provided insight into the regulatory principles underlying the once per cell cycle replication of the genome, as well as the relationship between S phase and mitosis...
  34. pmc WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase
    Jian Hu
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Genes Dev 22:866-71. 2008
    ..These results indicate that FBW5-DDB1-CUL4-ROC1 is an E3 ubiquitin ligase regulating TSC2 protein stability and TSC complex turnover...

Research Grants16

  1. NRSA IN GENETICS
    ROBERT DURONIO; Fiscal Year: 2007
    ..Essentially all students publish their dissertation research in peer-reviewed journals. ..
  2. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 1999
    ....
  3. Developmental Control of the Cell Cycle
    Robert J Duronio; Fiscal Year: 2010
    ..A detailed molecular description of cell cycle events during animal development is critical for our understanding of cell proliferation control, and how such control goes awry in diseases like cancer. ..
  4. Developmental Control of the Cell Cycle
    ROBERT DURONIO; Fiscal Year: 2009
    ..A detailed molecular description of cell cycle events during animal development is critical for our understanding of cell proliferation control, and how such control goes awry in diseases like cancer. ..
  5. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2007
    ....
  6. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2006
    ....
  7. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2005
    ....
  8. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2004
    ....
  9. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2003
    ....
  10. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2002
    ....
  11. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2001
    ....
  12. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2001
    ....
  13. GENETIC ANALYSIS OF E2F FUNCTION DURING DEVELOPMENT
    ROBERT DURONIO; Fiscal Year: 2000
    ....
  14. Developmental Control of the Cell Cycle
    Robert J Duronio; Fiscal Year: 2010
    ..In this proposal we focus on gene expression mechanisms that control the G1-S transition because this is when most cells decide whether to enter or to exit the cell cycle. ..