Gary Dunny

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. ncbi request reprint Cell-cell communication in gram-positive bacteria
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
    Annu Rev Microbiol 51:527-64. 1997
  2. ncbi request reprint Group II introns and expression of conjugative transfer functions in lactic acid bacteria
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, USA
    Antonie Van Leeuwenhoek 76:77-88. 1999
  3. ncbi request reprint Peptide pheromone-induced transfer of plasmid pCF10 in Enterococcus faecalis: probing the genetic and molecular basis for specificity of the pheromone response
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, 420 Delaware St SE, Minneapolis, MN 55455 0312, USA
    Peptides 22:1529-39. 2001
  4. pmc Biofilm growth alters regulation of conjugation by a bacterial pheromone
    Laura Cook
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Microbiol 81:1499-510. 2011
  5. pmc Enterococcal aggregation substance and binding substance are not major contributors to urinary tract colonization by Enterococcus faecalis in a mouse model of ascending unobstructed urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Infect Immun 72:2445-8. 2004
  6. pmc In vivo induction of virulence and antibiotic resistance transfer in Enterococcus faecalis mediated by the sex pheromone-sensing system of pCF10
    Helmut Hirt
    Department of Microbiology, University of Minnesota, Medical School, Minneapolis, Minnesota 55455, USA
    Infect Immun 70:716-23. 2002
  7. pmc A paracrine peptide sex pheromone also acts as an autocrine signal to induce plasmid transfer and virulence factor expression in vivo
    Josephine R Chandler
    Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:15617-22. 2005
  8. pmc Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis
    Ke Shi
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:18596-601. 2005
  9. pmc Pheromone-inducible conjugation in Enterococcus faecalis: a model for the evolution of biological complexity?
    Briana K Kozlowicz
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, MMC196, 420 Delaware St, SE, Minneapolis, MN 55455, USA
    Int J Med Microbiol 296:141-7. 2006
  10. pmc Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease
    Agathe Bourgogne
    Division of Infectious Disease, Center for the Study of Emerging and Re Emerging Pathogens, MSB 2 112, University of Texas Medical School, 6431 Fannin St, Houston, TX 77030, USA
    J Bacteriol 188:2875-84. 2006

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Cell-cell communication in gram-positive bacteria
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
    Annu Rev Microbiol 51:527-64. 1997
    ..The microbial processes controlled by extracellular signaling include the expression of virulence factors, the expression of gene transfer functions, and the production of antibiotics...
  2. ncbi request reprint Group II introns and expression of conjugative transfer functions in lactic acid bacteria
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, USA
    Antonie Van Leeuwenhoek 76:77-88. 1999
    ..The lactococcal Group II introns represent the first demonstrated genetically mobile prokaryotic retroelements, and they also have considerable potential as genetic engineering tools for Lactic Acid Bacteria (LAB) and other organisms...
  3. ncbi request reprint Peptide pheromone-induced transfer of plasmid pCF10 in Enterococcus faecalis: probing the genetic and molecular basis for specificity of the pheromone response
    G M Dunny
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, 420 Delaware St SE, Minneapolis, MN 55455 0312, USA
    Peptides 22:1529-39. 2001
    ..This communication reviews the evidence for this specificity and discusses current molecular and genetic approaches to defining the basis for specificity...
  4. pmc Biofilm growth alters regulation of conjugation by a bacterial pheromone
    Laura Cook
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Microbiol 81:1499-510. 2011
    ..These results may have important implications for development of chemotherapeutic agents to block resistance transfer and treat biofilm-related clinical infections...
  5. pmc Enterococcal aggregation substance and binding substance are not major contributors to urinary tract colonization by Enterococcus faecalis in a mouse model of ascending unobstructed urinary tract infection
    James R Johnson
    Mucosal and Vaccine Research Center, VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Infect Immun 72:2445-8. 2004
    ..These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family...
  6. pmc In vivo induction of virulence and antibiotic resistance transfer in Enterococcus faecalis mediated by the sex pheromone-sensing system of pCF10
    Helmut Hirt
    Department of Microbiology, University of Minnesota, Medical School, Minneapolis, Minnesota 55455, USA
    Infect Immun 70:716-23. 2002
    ..The data suggested that the mechanism of in vivo induction may involve interference of plasma with the normal function of the pheromone peptide and its inhibitor...
  7. pmc A paracrine peptide sex pheromone also acts as an autocrine signal to induce plasmid transfer and virulence factor expression in vivo
    Josephine R Chandler
    Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:15617-22. 2005
    ..Although cCF10 has traditionally been viewed as an intercellular signal, these results show that pCF10 has also adapted cCF10 as an autocrine signal that activates expression of virulence and conjugation functions...
  8. pmc Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis
    Ke Shi
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:18596-601. 2005
    ..Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression...
  9. pmc Pheromone-inducible conjugation in Enterococcus faecalis: a model for the evolution of biological complexity?
    Briana K Kozlowicz
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, MMC196, 420 Delaware St, SE, Minneapolis, MN 55455, USA
    Int J Med Microbiol 296:141-7. 2006
    ....
  10. pmc Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease
    Agathe Bourgogne
    Division of Infectious Disease, Center for the Study of Emerging and Re Emerging Pathogens, MSB 2 112, University of Texas Medical School, 6431 Fannin St, Houston, TX 77030, USA
    J Bacteriol 188:2875-84. 2006
    ....
  11. pmc Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10
    Jack H Staddon
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455 0312, USA
    Plasmid 56:102-11. 2006
    ..Therefore, this transfer system may have applications for gene delivery to a variety of poorly-transformed bacteria...
  12. pmc Development of a host-genotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalis
    Christopher J Kristich
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Plasmid 57:131-44. 2007
    ..Thus, the advanced tools for genetic manipulation of E. faecalis reported here enable efficient and sophisticated genetic analysis of these important pathogens...
  13. pmc Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides
    Briana K Kozlowicz
    Department of Microbiology, 1460 Mayo Memorial Building, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Microbiol 62:958-69. 2006
    ..We now show that both of these peptides interact with the same binding pocket of PrgX, but they differentially alter the conformation of the protein and its oligomerization state, resulting in opposing biological activities...
  14. pmc Analysis of the amino acid sequence specificity determinants of the enterococcal cCF10 sex pheromone in interactions with the pheromone-sensing machinery
    Kathryn R Fixen
    Department of Microbiology, University of Minnesota, MMC 196, Minneapolis, MN 55455, USA
    J Bacteriol 189:1399-406. 2007
    ..The results provide functional data to complement ongoing structural studies of PrgX and increase our understanding of the functional interactions of cCF10 and iCF10 with the pheromone-sensing machinery of pCF10...
  15. pmc Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01
    Yuqing Chen
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Microbiol 63:1549-64. 2007
    ..Specificity of conjugative DNA processing in these plasmids involves both DNA-protein and protein-protein interactions...
  16. pmc The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolution
    Gary M Dunny
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    Philos Trans R Soc Lond B Biol Sci 362:1185-93. 2007
    ..In addition, the system may serve as a useful bacterial model for addressing the evolution of biological complexity...
  17. pmc A eukaryotic-type Ser/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistence
    Christopher J Kristich
    Department of Microbiology and Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 104:3508-13. 2007
    ....
  18. pmc Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgY
    Josephine R Chandler
    Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
    J Bacteriol 190:1172-83. 2008
    ..Despite evidence that both PrgY and Eep associate with cCF10 in or near the membrane, results presented here indicate that these two proteins function independently...
  19. pmc Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate
    Yuqing Chen
    Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
    J Bacteriol 190:3632-45. 2008
    ....
  20. doi request reprint Multicellular behavior in bacteria: communication, cooperation, competition and cheating
    Gary M Dunny
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455 0312, USA
    Bioessays 30:296-8. 2008
    ..Here we discuss the significance of two recent studies of the phenomenon of "cheating" mutants and their exploitation of cooperating microbial populations of Pseudomonas aeruginosa...
  21. pmc Development and use of an efficient system for random mariner transposon mutagenesis to identify novel genetic determinants of biofilm formation in the core Enterococcus faecalis genome
    Christopher J Kristich
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Appl Environ Microbiol 74:3377-86. 2008
    ..These results provide significant new information about the genetics of enterococcal biofilm formation and demonstrate the general utility of our transposon system for functional genomic analysis of E. faecalis...
  22. pmc Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutant
    Christopher J Kristich
    420 Delaware St S E, MMC196, Minneapolis, MN 55455, USA
    Appl Environ Microbiol 71:5837-49. 2005
    ..faecalis cells required for high-frequency conjugative plasmid transfer in liquid matings. The system of markerless exchange reported here will facilitate detailed genetic analysis of these important pathogens...
  23. pmc Specific control of endogenous cCF10 pheromone by a conserved domain of the pCF10-encoded regulatory protein PrgY in Enterococcus faecalis
    Josephine R Chandler
    Department of Microbiology, University of Minnesota, Minneapolis, 55455, USA
    J Bacteriol 187:4830-43. 2005
    ..The combined genetic and physiological data suggest that PrgY may sequester or inactivate cCF10 as it is released from the membrane...
  24. pmc ccfA, the genetic determinant for the cCF10 peptide pheromone in Enterococcus faecalis OG1RF
    Michelle H Antiporta
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455 0312, USA
    J Bacteriol 184:1155-62. 2002
    ..faecalis and also resulted in cCF10 production by Lactococcus lactis, a non-pheromone producer. Site-directed mutagenesis of the ccfA sequence encoding the cCF10 peptide confirmed that ccfA was a functional genetic determinant for cCF10...
  25. ncbi request reprint Two targets in pCF10 DNA for PrgX binding: their role in production of Qa and prgX mRNA and in regulation of pheromone-inducible conjugation
    Taeok Bae
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 315:995-1007. 2002
    ..On the basis of these results, we propose that both DNA binding sites are required for the autoregulation of PrgX expression and for positive regulation of Qa RNA...
  26. pmc Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins
    Christopher M Waters
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 185:3613-23. 2003
    ..These new functions attributed to GelE suggest that it acts to increase the dissemination of E. faecalis in high-density environments...
  27. pmc The aggregation domain of aggregation substance, not the RGD motifs, is critical for efficient internalization by HT-29 enterocytes
    Christopher M Waters
    Department of Microbiology, University of Minnesota Medical School, 1420 Delaware Street SE, Minneapolis, MN 55455, USA
    Infect Immun 71:5682-9. 2003
    ..These data show that Asc10 directs internalization of E. faecalis into HT-29 enterocytes through a non-RGD-dependent mechanism...
  28. ncbi request reprint Characterization of cis-acting prgQ mutants: evidence for two distinct repression mechanisms by Qa RNA and PrgX protein in pheromone-inducible enterococcal plasmid pCF10
    Taeok Bae
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Microbiol 51:271-81. 2004
    ..Thus, even though the synthesis of these negative regulators is coupled, they each act independently on separate targets to regulate expression of conjugation functions...
  29. pmc Esp-independent biofilm formation by Enterococcus faecalis
    Christopher J Kristich
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 186:154-63. 2004
    ..faecalis. In summary, E. faecalis forms complex biofilms by a process that is sensitive to environmental conditions and does not require the Esp surface protein...
  30. pmc A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis
    Joanna R Klein
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 186:1991-8. 2004
    ..Once the system was validated for the engineered mutants, random mutagenesis of the intron followed by genetic and molecular screening for splicing defects resulted in identification of point mutations that affect splicing...
  31. pmc Conserved target for group II intron insertion in relaxase genes of conjugative elements of gram-positive bacteria
    Jack H Staddon
    Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Bacteriol 186:2393-401. 2004
    ..Additional support for this mechanism comes from sequence analysis of the insertion sites of the E.c.I4 family of bacterial group II introns...
  32. ncbi request reprint Enterococcal peptide sex pheromones: synthesis and control of biological activity
    Josephine R Chandler
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, 420 Delaware Street SE, Minneapolis, MN 55455 0312, USA
    Peptides 25:1377-88. 2004
    ....
  33. ncbi request reprint Enterococcus faecalis pheromone-responsive protein PrgX: genetic separation of positive autoregulatory functions from those involved in negative regulation of conjugative plasmid transfer
    Briana K Kozlowicz
    Department of Microbiology, 1460 Mayo Memorial Bldg, University of Minnesota, MN 55455, USA
    Mol Microbiol 54:520-32. 2004
    ..We propose a new model for the mechanism used by PrgX for regulation of the prgQ promoter, PrgX autoregulation, and Qa RNA processing...
  34. ncbi request reprint High-resolution visualization of the microbial glycocalyx with low-voltage scanning electron microscopy: dependence on cationic dyes
    Stanley L Erlandsen
    Deptartment of Genetics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Histochem Cytochem 52:1427-35. 2004
    ....
  35. ncbi request reprint Group effort in toxin synthesis
    Gary M Dunny
    Nature 415:33-4. 2002
  36. pmc Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction
    Helmut Hirt
    Department of Microbiology, Medical School, University of Minnesota, Minneapolis, USA
    J Bacteriol 187:1044-54. 2005
    ..This observation could have relevance for the virulence of E. faecalis...
  37. ncbi request reprint Bacterial group II introns and their association with mobile genetic elements
    Joanna R Klein
    Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg Box 196, 420 Delaware St SE, Minneapolis, MN 55455 0312, USA
    Front Biosci 7:d1843-56. 2002
    ..Limited functional data is available for most of these introns, but sequence analysis points out several common themes, most notably that bacterial group II introns are almost always carried on mobile genetic elements...
  38. ncbi request reprint Formation of vegetations during infective endocarditis excludes binding of bacterial-specific host antibodies to Enterococcus faecalis
    John K McCormick
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    J Infect Dis 185:994-7. 2002
    ....
  39. pmc Quantitative analysis of group II intron expression and splicing in Lactococcus lactis
    Yuqing Chen
    Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
    Appl Environ Microbiol 71:2576-86. 2005
    ..Interestingly, LtrB protein showed a tendency to function in cis on its oriT target. The low level of ltrB transcript and relatively inefficient splicing of the intron may limit Ll.ltrB mobility and dissemination in nature...

Research Grants42

  1. Genetic Functions of an Enterococcal R Factor
    Gary M Dunny; Fiscal Year: 2010
    ..The basic knowledge gained from this research may provide new insights into the development of more effective forms of antimicrobial chemotherapy. ..
  2. Biofilms and Enterococcus faecalis Biology
    Gary M Dunny; Fiscal Year: 2011
    ..If there is functional conservation of these genes, it could speed the development of new antimicrobial drugs or vaccines. ..
  3. Genetic Functions of an Enterococcal R Factor
    Gary Dunny; Fiscal Year: 2004
    ..These steps include post-transcriptional activation of transfer gene expression, and conjugative DNA processing. ..
  4. CELL WALL COMPONENTS AND ENTERCOCCAL ENDOCARDITIS
    Gary Dunny; Fiscal Year: 2002
    ..3) Determine the key structural features of AS and EBS required for the enhancement of virulence by these molecules. 4) Determine the mechanism by which expression of AS is induced in vivo. ..
  5. Cell Wall Components' Role in Enterococcal Endocarditis
    Gary Dunny; Fiscal Year: 2006
    ..faecalis virulence. 2) Determine the functional domains of Asc10 that confer the activities identified in Aim 1. 3) Examine the expression of bacterial and host genes during the course of infections. ..
  6. Biofilms and Enterococcus faecalis Biology
    Gary Dunny; Fiscal Year: 2009
    ..3) To analyze the cellular and molecular structure of E. faecalis biofilms using high resolution scanning electron microscopy. 4) To test the virulence of selected biofilm-deficient mutants in experimental endocarditis. ..
  7. Genetic Functions of an Enterococcal R Factor
    Gary Dunny; Fiscal Year: 2009
    ..3. Determine the mechanism of action of PrgY, a novel membrane protein that acts at the cell surface to prevent self-induction in donor cells by endogenously produced, cell-associated pheromone. ..
  8. Genetic Functions of an Enterococcal R Factor
    Gary Dunny; Fiscal Year: 2007
    ..3. Determine the mechanism of action of PrgY, a novel membrane protein that acts at the cell surface to prevent self-induction in donor cells by endogenously produced, cell-associated pheromone. ..
  9. A BACTERIAL GROUP II INTRON INVOLVED IN CONJUGATION
    Gary Dunny; Fiscal Year: 2002
    ..Identification and analysis of small molecule splicing inhibitors using a splicing-dependent conjugation assay. ..
  10. GENETIC FUNCTIONS OF A CONJUGATIVE STREPTOCOCCAL R FACTO
    Gary Dunny; Fiscal Year: 1993
    ..The results should improve our understanding of the continuing spread of antibiotic resistance in streptococci. This system is also a useful model for studying intercellular chemical communication and the bacterial cell surface...
  11. GENETIC FUNCTIONS OF CONJUGATIVE STREPTOCOCCAL R FACTOR
    Gary Dunny; Fiscal Year: 1999
    ....