John E Dueber
Affiliation: University of California
- Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assayMichael E Lee
The UC Berkeley and UCSF Graduate Program in Bioengineering, Berkeley, CA 94720, USA, Department of Bioengineering, University of California, Berkeley, CA 94720, USA, Energy Biosciences Institute, Berkeley, CA 94720, USA, Department of Industrial Engineering and Operations Research, University of California, Berkeley, CA 94720, USA and Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, CA 94720, USA
Nucleic Acids Res 41:10668-78. 2013..This generalizable method should prove useful in engineering new pathways for the sustainable production of small molecules. ..
- Synthetic protein scaffolds provide modular control over metabolic fluxJohn E Dueber
California Institute of Quantitative Biomedical Research QB3, University of California, Berkeley, California, USA
Nat Biotechnol 27:753-9. 2009..5 g/l) without the synthetic complex. These strategies should prove generalizeable to other metabolic pathways and programmable for fine-tuning pathway flux...
- Engineering robust control of two-component system phosphotransfer using modular scaffoldsWeston R Whitaker
The University of California, Berkeley and University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 109:18090-5. 2012..Thus, we demonstrate that design principles inspired by the complex signal-transduction pathways of eukaryotes may be generalized, abstracted, and applied to prokaryotes using well-characterized parts...
- BglBricks: A flexible standard for biological part assemblyJ Christopher Anderson
Department of Bioengineering, University of California, Berkeley, CA 94720, USA
J Biol Eng 4:1. 2010..These tools will provide a foundation from which to transform genetic engineering from a technically intensive art into a purely design-based discipline...
- Reprogramming control of an allosteric signaling switch through modular recombinationJohn E Dueber
Program in Biological Sciences, University of California, San Francisco, CA 94143 2240, USA
Science 301:1904-8. 2003..Synthetic switch proteins were created with diverse gating behaviors in response to nonphysiological inputs. Thus, this type of modular framework can facilitate the evolution or engineering of cellular signaling circuits...
- Engineering synthetic signaling proteins with ultrasensitive input/output controlJohn E Dueber
Program in Biological Sciences, University of California, San Francisco, California 94158 2517, USA
Nat Biotechnol 25:660-2. 2007..By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch...
- The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalencyNathan A Sallee
Graduate Program in Chemistry and Chemical Biology, University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA
Nature 454:1005-8. 2008..Thus, this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine...