John E Dueber
Affiliation: University of California
- Synthetic protein scaffolds provide modular control over metabolic fluxJohn E Dueber
California Institute of Quantitative Biomedical Research QB3, University of California, Berkeley, California, USA
Nat Biotechnol 27:753-9. 2009..5 g/l) without the synthetic complex. These strategies should prove generalizeable to other metabolic pathways and programmable for fine-tuning pathway flux...
- BglBricks: A flexible standard for biological part assemblyJ Christopher Anderson
Department of Bioengineering, University of California, Berkeley, CA 94720, USA
J Biol Eng 4:1. 2010..These tools will provide a foundation from which to transform genetic engineering from a technically intensive art into a purely design-based discipline...
- Reprogramming control of an allosteric signaling switch through modular recombinationJohn E Dueber
Program in Biological Sciences, University of California, San Francisco, CA 94143-2240, USA
Science 301:1904-8. 2003..Synthetic switch proteins were created with diverse gating behaviors in response to nonphysiological inputs. Thus, this type of modular framework can facilitate the evolution or engineering of cellular signaling circuits...
- Engineering synthetic signaling proteins with ultrasensitive input/output controlJohn E Dueber
Program in Biological Sciences, University of California, San Francisco, California 94158 2517, USA
Nat Biotechnol 25:660-2. 2007..By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch...
- The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalencyNathan A Sallee
Graduate Program in Chemistry and Chemical Biology, University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA
Nature 454:1005-8. 2008..Thus, this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine...