J G Drachman

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Dissecting the thrombopoietin receptor: functional elements of the Mpl cytoplasmic domain
    J G Drachman
    Division of Hematology, University of Washington Medical Center, Seattle 98195, USA
    Proc Natl Acad Sci U S A 94:2350-5. 1997
  2. ncbi request reprint Thrombopoietin signal transduction requires functional JAK2, not TYK2
    J G Drachman
    Division of Hematology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 274:13480-4. 1999
  3. ncbi request reprint Autosomal dominant thrombocytopenia: incomplete megakaryocyte differentiation and linkage to human chromosome 10
    J G Drachman
    Puget Sound Blood Center, Seattle, WA 98104, USA
    Blood 96:118-25. 2000
  4. ncbi request reprint A structure-function analysis of serine/threonine phosphorylation of the thrombopoietin receptor, c-Mpl
    Y Miyakawa
    Divisions of Hematology and Cardiology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 275:32214-9. 2000
  5. ncbi request reprint The c-Mpl ligand (thrombopoietin) stimulates tyrosine phosphorylation of Jak2, Shc, and c-Mpl
    J G Drachman
    Department of Medicine, University of Washington, School of Medicine, Seattle 98195
    J Biol Chem 270:4979-82. 1995
  6. ncbi request reprint Membrane localization is not required for Mpl function in normal hematopoietic cells
    K G Otto
    Department of Medicine, Division of Hematology, University of Washington, Seattle WA, USA
    Blood 98:2077-83. 2001
  7. ncbi request reprint X-linked thrombocytopenia caused by a novel mutation of GATA-1
    M G Mehaffey
    Puget Sound Blood Center and Program, Seattle, WA 98104, USA
    Blood 98:2681-8. 2001
  8. ncbi request reprint Increased megakaryocytopoiesis in Lyn-deficient mice
    B J Lannutti
    Puget Sound Blood Center, Seattle, WA, USA
    Oncogene 25:3316-24. 2006
  9. ncbi request reprint The molecular and cellular biology of thrombopoietin: the primary regulator of platelet production
    Kenneth Kaushansky
    Division of Hematology, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, Washington, WA 98195, USA
    Oncogene 21:3359-67. 2002
  10. ncbi request reprint Congenital amegakaryocytic thrombocytopenia in three siblings: molecular analysis of atypical clinical presentation
    Manish J Gandhi
    Puget Sound Blood Center, Seattle, WAash, USA Division of Hematology, University of Washington School of Medicine, Seattle, WA 98104, USA
    Exp Hematol 33:1215-21. 2005

Collaborators

  • B Gallis
  • Kenneth Kaushansky
  • GAIL JARVIK
  • C L Balduini
  • Virginia C Broudy
  • Amy E Geddis
  • Rosana F Marta
  • Manish J Gandhi
  • Brian J Lannutti
  • Jo Anna Reems
  • Noel Blake
  • Mi Hyun Shim
  • B J Lannutti
  • Paula G Heller
  • Carrie L Cummings
  • Jennifer N Luthi
  • Debra D Dahlen
  • K G Otto
  • M G Mehaffey
  • Y Miyakawa
  • J Minear
  • N Blake
  • Felisa C Molinas
  • Thomas W Pendergrass
  • C Anthony Blau
  • Laura I Kornblihtt
  • Kenji Ihara
  • Carlos J Pirola
  • Ana C Glembotsky
  • David Thorning
  • Carrie C Cummings
  • Aubree Hoover
  • M J Gandhi
  • J N Ihle
  • C A Blau
  • M Crossley
  • E Parganas
  • A L Newton
  • J N Luthi
  • N L Lin
  • A Kaushansky

Detail Information

Publications22

  1. pmc Dissecting the thrombopoietin receptor: functional elements of the Mpl cytoplasmic domain
    J G Drachman
    Division of Hematology, University of Washington Medical Center, Seattle 98195, USA
    Proc Natl Acad Sci U S A 94:2350-5. 1997
    ....
  2. ncbi request reprint Thrombopoietin signal transduction requires functional JAK2, not TYK2
    J G Drachman
    Division of Hematology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 274:13480-4. 1999
    ..These data indicate that JAK2 is an essential component of Mpl signaling and that, in the absence of JAK2, TYK2 is incapable of initiating TPO-induced tyrosine phosphorylation...
  3. ncbi request reprint Autosomal dominant thrombocytopenia: incomplete megakaryocyte differentiation and linkage to human chromosome 10
    J G Drachman
    Puget Sound Blood Center, Seattle, WA 98104, USA
    Blood 96:118-25. 2000
    ..68 (at theta = 0). By recruiting additional family members, the genomic region was narrowed to 17 centimorgans. We conclude that a gene in this locus plays an important role in megakaryocyte endomitosis and terminal maturation...
  4. ncbi request reprint A structure-function analysis of serine/threonine phosphorylation of the thrombopoietin receptor, c-Mpl
    Y Miyakawa
    Divisions of Hematology and Cardiology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 275:32214-9. 2000
    ..However, the mutation of Ser-18 to Ala nearly abrogates TPO-induced proliferation and co-precipitation of JAK2 with Mpl. This study provides the first systematic analysis of the role of Ser residues in c-Mpl signaling...
  5. ncbi request reprint The c-Mpl ligand (thrombopoietin) stimulates tyrosine phosphorylation of Jak2, Shc, and c-Mpl
    J G Drachman
    Department of Medicine, University of Washington, School of Medicine, Seattle 98195
    J Biol Chem 270:4979-82. 1995
    ..Additionally, in light of its late physical association with c-Mpl following ligand binding, Jak2 may not be the initiating tyrosine kinase in the thrombopoietin-induced signaling cascade...
  6. ncbi request reprint Membrane localization is not required for Mpl function in normal hematopoietic cells
    K G Otto
    Department of Medicine, Division of Hematology, University of Washington, Seattle WA, USA
    Blood 98:2077-83. 2001
    ..These data show that the activation of Mpl independent of its normal plasma membrane location can support production of the full range of normal hematopoietic progenitor cells in vitro...
  7. ncbi request reprint X-linked thrombocytopenia caused by a novel mutation of GATA-1
    M G Mehaffey
    Puget Sound Blood Center and Program, Seattle, WA 98104, USA
    Blood 98:2681-8. 2001
    ..These findings help describe a novel mutation of GATA-1 in humans as a cause of X-linked thrombocytopenia, and they confirm the vital role played by this transcription factor during in vivo megakaryocyte development...
  8. ncbi request reprint Increased megakaryocytopoiesis in Lyn-deficient mice
    B J Lannutti
    Puget Sound Blood Center, Seattle, WA, USA
    Oncogene 25:3316-24. 2006
    ..This is the first direct evidence using primary MKs from Lyn-deficient mice that confirms our prior data from cell lines that Lyn kinase is a negative regulator of TPO signaling...
  9. ncbi request reprint The molecular and cellular biology of thrombopoietin: the primary regulator of platelet production
    Kenneth Kaushansky
    Division of Hematology, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, Washington, WA 98195, USA
    Oncogene 21:3359-67. 2002
    ..This review attempts to distill what has been learned of the molecular and cellular biology of TPO and its receptor during the past several years, and links this information to several new insights into human disease and its treatment...
  10. ncbi request reprint Congenital amegakaryocytic thrombocytopenia in three siblings: molecular analysis of atypical clinical presentation
    Manish J Gandhi
    Puget Sound Blood Center, Seattle, WAash, USA Division of Hematology, University of Washington School of Medicine, Seattle, WA 98104, USA
    Exp Hematol 33:1215-21. 2005
    ..We evaluated this pedigree to understand the unusually late presentation of congenital amegakaryocytic thrombocytopenia (CAMT)...
  11. ncbi request reprint A novel strategy for generating platelet-like fragments from megakaryocytic cell lines and human progenitor cells
    Manish J Gandhi
    Puget Sound Blood Center, 921 Terry Avenue, Seattle, WA 98104, USA
    Blood Cells Mol Dis 35:70-3. 2005
    ..These studies provide evidence that SU6656 promotes megakaryocytic differentiation and thrombopoiesis in vitro...
  12. ncbi request reprint Low Mpl receptor expression in a pedigree with familial platelet disorder with predisposition to acute myelogenous leukemia and a novel AML1 mutation
    Paula G Heller
    Division of Hematology, Instituto de Investigaciones Medicas Alfredo Lanari, University of Buenos Aires, Argentina
    Blood 105:4664-70. 2005
    ..In conclusion, we identified a C-terminal AML1 mutation that leads to a decrease in Mpl receptor expression, providing a potential explanation for thrombocytopenia in this FPD/AML pedigree...
  13. pmc Induction of polyploidization in leukemic cell lines and primary bone marrow by Src kinase inhibitor SU6656
    Brian J Lannutti
    Puget Sound Blood Center, 921 Terry Ave, Seattle, WA 98104, USA
    Blood 105:3875-8. 2005
    ..Our data suggest that SU6656 might be useful as a differentiation-inducing agent for MKs and is an important tool for understanding the molecular basis of MK endomitosis...
  14. ncbi request reprint Gene expression profile of primary human CD34+CD38lo cells differentiating along the megakaryocyte lineage
    Mi Hyun Shim
    Puget Sound Blood Center, Seattle, WA 98104, USA
    Exp Hematol 32:638-48. 2004
    ..To identify genes involved in megakaryopoiesis, high-density oligonucleotide microarrays were used to compare transcript profiles from undifferentiated CD34+CD38lo cells and culture-derived megakaryocytes (MKs)...
  15. ncbi request reprint Lyn tyrosine kinase regulates thrombopoietin-induced proliferation of hematopoietic cell lines and primary megakaryocytic progenitors
    Brian J Lannutti
    Puget Sound Blood Center, Seattle, WA 98104 1256, USA
    Blood 103:3736-43. 2004
    ..These data suggest that SFKs modify the rate of TPO-induced proliferation and are likely to affect cell cycle regulation during megakaryocytopoiesis...
  16. ncbi request reprint Identification and activation of Src family kinases in primary megakaryocytes
    Brian J Lannutti
    Puget Sound Blood Center, 921 Terry Avenue, Seattle, WA 98104 1256, USA
    Exp Hematol 31:1268-74. 2003
    ..In this study, we examine which of the eight Src family members are expressed in primary megakaryocytes (MKs) and determine which of these become activated in response to TPO...
  17. ncbi request reprint Studies with chimeric Mpl/JAK2 receptors indicate that both JAK2 and the membrane-proximal domain of Mpl are required for cellular proliferation
    Jonathan G Drachman
    Puget Sound Blood Center, Seattle, Washington 98104, USA
    J Biol Chem 277:23544-53. 2002
    ..Although the nature of the signal transduction pathway is not yet known, this second proliferative event is likely to regulate c-myc expression...
  18. ncbi request reprint Internalization of the thrombopoietin receptor is regulated by 2 cytoplasmic motifs
    Debra D Dahlen
    Division of Hematology, University of Washington, Seattle, WA 98104, USA
    Blood 102:102-8. 2003
    ..These data help identify the receptor motifs involved in TPO-induced internalization of Mpl and suggest that Mpl translocation may be necessary for normal cellular proliferation...
  19. ncbi request reprint X-linked thrombocytopenia caused by a mutation in the Wiskott-Aldrich syndrome (WAS) gene that disrupts interaction with the WAS protein (WASP)-interacting protein (WIP)
    Jennifer N Luthi
    Puget Sound Blood Center and Program, 921 Terry Avenue, Seattle, WA 98104, USA
    Exp Hematol 31:150-8. 2003
    ..Despite the absence of eczema or immunodeficiency, a mutation of the Wiskott-Aldrich syndrome (WAS) gene was suspected because of the presence of microthrombocytes...
  20. ncbi request reprint FLJ14813 missense mutation: a candidate for autosomal dominant thrombocytopenia on human chromosome 10
    Manish J Gandhi
    Puget Sound Blood Center, Seattle, Wash 98104, USA
    Hum Hered 55:66-70. 2003
    ..A substitution of cytosine for guanidine (G to C) at nucleotide position 565 was present in all thrombocytopenic family members, causing a predicted substitution of aspartic acid for glutamic acid (E167D) in exon four...
  21. ncbi request reprint Role of splenectomy in inherited thrombocytopenias
    Carlo L Balduini
    Blood 104:1227; author reply 1227-8. 2004
  22. ncbi request reprint Inherited thrombocytopenia: when a low platelet count does not mean ITP
    Jonathan G Drachman
    Puget Sound Blood Center, 921 Terry Ave, Seattle, WA 98104, USA
    Blood 103:390-8. 2004
    ..The mutated genes responsible for each syndrome are reviewed as well as the potential implications for using gene therapy or gene repair in the future...