Albert D Donnenberg

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. doi request reprint Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies
    Albert D Donnenberg
    Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2 42 Research Pavilion, Pittsburgh, PA 15213, USA
    Regen Med 6:701-6. 2011
  2. ncbi request reprint Rare-event analysis in flow cytometry
    Albert D Donnenberg
    University of Pittsburgh School of Medicine, Hillman Cancer Research Center Suite2 42c, 5117 Center Avenue, Pittsburgh, PA 15213 2582, USA
    Clin Lab Med 27:627-52, viii. 2007
  3. doi request reprint Intra-operative preparation of autologous bone marrow-derived CD34-enriched cellular products for cardiac therapy
    Albert D Donnenberg
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytotherapy 13:441-8. 2011
  4. ncbi request reprint Measurement of basal, substrate induced and total P-glycoprotein activity in bronchoalveolar lavage T-cell subsets
    V S Donnenberg
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytometry A 57:75-85. 2004
  5. pmc Tumorigenic stem and progenitor cells: implications for the therapeutic index of anti-cancer agents
    Vera S Donnenberg
    Hillman Cancer Center Research Pavilion, 5117 Centre Ave, Pittsburgh, PA 15213, United States
    J Control Release 122:385-91. 2007
  6. doi request reprint Development and validation of a procedure to isolate viable bone marrow cells from the vertebrae of cadaveric organ donors for composite organ grafting
    Vijay S Gorantla
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytotherapy 14:104-13. 2012
  7. pmc Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates
    Ludovic Zimmerlin
    University of Pittsburgh Cancer Institute, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Tissue Eng Part A 17:93-106. 2011
  8. doi request reprint Human adipose stromal vascular cell delivery in a fibrin spray
    Ludovic Zimmerlin
    Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cytotherapy 15:102-8. 2013
  9. doi request reprint Statistical classification of multivariate flow cytometry data analyzed by manual gating: stem, progenitor, and epithelial marker expression in nonsmall cell lung cancer and normal lung
    Daniel P Normolle
    Department of Biostatistics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15213, USA
    Cytometry A 83:150-60. 2013
  10. doi request reprint Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
    Vera S Donnenberg
    Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cytometry A 83:141-9. 2013

Research Grants

  1. Imaging Flow Cytometer
    Albert Donnenberg; Fiscal Year: 2006

Collaborators

Detail Information

Publications32

  1. doi request reprint Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies
    Albert D Donnenberg
    Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2 42 Research Pavilion, Pittsburgh, PA 15213, USA
    Regen Med 6:701-6. 2011
    ....
  2. ncbi request reprint Rare-event analysis in flow cytometry
    Albert D Donnenberg
    University of Pittsburgh School of Medicine, Hillman Cancer Research Center Suite2 42c, 5117 Center Avenue, Pittsburgh, PA 15213 2582, USA
    Clin Lab Med 27:627-52, viii. 2007
    ..A growing number of flow cytometry-based assays depend on rare-event detection for basic science and clinical applications...
  3. doi request reprint Intra-operative preparation of autologous bone marrow-derived CD34-enriched cellular products for cardiac therapy
    Albert D Donnenberg
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytotherapy 13:441-8. 2011
    ..In this report we present a methodology for intra-operative preparation of CD34(+) selected autologous bone marrow for autologous use in patients receiving coronary artery bypass grafts or left ventricular assist devices...
  4. ncbi request reprint Measurement of basal, substrate induced and total P-glycoprotein activity in bronchoalveolar lavage T-cell subsets
    V S Donnenberg
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytometry A 57:75-85. 2004
    ..Our objectives were to evaluate P-gp activity in T cells freshly isolated from bronchoalveolar lavage (BAL) and to develop a strategy to distinguish between basal, in vitro substrate-induced, and total P-gp activities...
  5. pmc Tumorigenic stem and progenitor cells: implications for the therapeutic index of anti-cancer agents
    Vera S Donnenberg
    Hillman Cancer Center Research Pavilion, 5117 Centre Ave, Pittsburgh, PA 15213, United States
    J Control Release 122:385-91. 2007
    ..The therapeutic index of antineoplastics thus becomes one of sensitivity of cancer and normal stem cells, which are protected by the same mechanisms...
  6. doi request reprint Development and validation of a procedure to isolate viable bone marrow cells from the vertebrae of cadaveric organ donors for composite organ grafting
    Vijay S Gorantla
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cytotherapy 14:104-13. 2012
    ....
  7. pmc Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates
    Ludovic Zimmerlin
    University of Pittsburgh Cancer Institute, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Tissue Eng Part A 17:93-106. 2011
    ..Our data indicate that ASC enhance the growth of active, but not resting tumor cells. Thus, reconstructive therapy utilizing ASC-augmented whole fat should be postponed until there is no evidence of active disease...
  8. doi request reprint Human adipose stromal vascular cell delivery in a fibrin spray
    Ludovic Zimmerlin
    Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cytotherapy 15:102-8. 2013
    ..We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system...
  9. doi request reprint Statistical classification of multivariate flow cytometry data analyzed by manual gating: stem, progenitor, and epithelial marker expression in nonsmall cell lung cancer and normal lung
    Daniel P Normolle
    Department of Biostatistics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15213, USA
    Cytometry A 83:150-60. 2013
    ..We anticipate that the methods described here will be broadly applicable to a variety of multidimensional cytometry problems...
  10. doi request reprint Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
    Vera S Donnenberg
    Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cytometry A 83:141-9. 2013
    ..The results of bivariate analysis show a striking similarity between the expression of stem/progenitor markers on lung tumor and adjacent tumor-free lung...
  11. ncbi request reprint P-glycoprotein activity is decreased in CD4+ but not CD8+ lung allograft-infiltrating T cells during acute cellular rejection
    Vera S Donnenberg
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Transplantation 77:1699-706. 2004
    ..Modulation of P-glycoprotein (P-gp) activity in graft-infiltrating T cells may alter their susceptibility to immunosuppression...
  12. doi request reprint Upper-extremity transplantation using a cell-based protocol to minimize immunosuppression
    Stefan Schneeberger
    Department of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Ann Surg 257:345-51. 2013
    ..To minimize maintenance immunosuppression in upper-extremity transplantation to favor the risk-benefit balance of this procedure...
  13. doi request reprint Measurement of multiple drug resistance transporter activity in putative cancer stem/progenitor cells
    Vera S Donnenberg
    Hillman Cancer Center, Pittsburgh, PA, USA
    Methods Mol Biol 568:261-79. 2009
    ....
  14. doi request reprint Adipogenic potential of adipose stem cell subpopulations
    Han Li
    Division of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Plast Reconstr Surg 128:663-72. 2011
    ..The aim of this study was to define distinct populations within the stromal vascular fraction based on surface marker expression, and to evaluate the ability of each cell type to differentiate to mature adipocytes...
  15. pmc KIT (CD117) expression in a subset of non-small cell lung carcinoma (NSCLC) patients
    Albert D Donnenberg
    Hillman Cancer Center, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e52885. 2012
    ..We conclude that NSCLC tumors divide into CD117(low) and CD117(high). Overexpression of CD117 in CD117(high) NSCLC supports exploring KIT as a therapeutic target in this subset of patients...
  16. ncbi request reprint Identification, rare-event detection and analysis of dendritic cell subsets in broncho-alveolar lavage fluid and peripheral blood by flow cytometry
    Vera S Donnenberg
    University of Pittsburgh Cancer Institute, Department of Surgery, The Hillman Cancer Center, 5117 Centre Avenue, Pittsburgh PA 15213, USA
    Front Biosci 8:s1175-80. 2003
    ....
  17. doi request reprint Localization of CD44 and CD90 positive cells to the invasive front of breast tumors
    Vera S Donnenberg
    University of Pittsburgh Cancer Center, Pittsburgh, Pennsylvania, USA
    Cytometry B Clin Cytom 78:287-301. 2010
    ..A variety of markers have been proposed to identify breast cancer stem cells. Here, we used immunohistostaining and flow cytometry to analyze their interrelationships and to sort cells for tumorigenicity studies...
  18. doi request reprint Rare event detection and analysis in flow cytometry: bone marrow mesenchymal stem cells, breast cancer stem/progenitor cells in malignant effusions, and pericytes in disaggregated adipose tissue
    Ludovic Zimmerlin
    Division of Hematology Oncology, Department of Medicine, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA
    Methods Mol Biol 699:251-73. 2011
    ....
  19. doi request reprint Stromal vascular progenitors in adult human adipose tissue
    Ludovic Zimmerlin
    Department of Medicine, Division of Hematology Oncology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
    Cytometry A 77:22-30. 2010
    ..A CD146+/CD34+ subset detected by flow cytometry at low frequency suggests a population transitional between pericytes and SA-ASC...
  20. ncbi request reprint Multiple drug resistance in cancer revisited: the cancer stem cell hypothesis
    Vera S Donnenberg
    Department of Surgery, Division of Thoracic Surgery, University of Pittsburgh Cancer Institute, Pennsilvania, USA
    J Clin Pharmacol 45:872-7. 2005
    ..In the context of this hypothesis, the authors review the discovery of MDR transporters in cancer and normal stem cells and the failure of MDR reversal agents to increase the therapeutic index of substrate antineoplastic agents...
  21. pmc Regenerative therapy after cancer: what are the risks?
    Vera S Donnenberg
    University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    Tissue Eng Part B Rev 16:567-75. 2010
    ....
  22. ncbi request reprint CD34 affinity pheresis attenuates a surge among circulating progenitor cells following vascular injury
    Adriana Harbuzariu
    Division of Vascular Surgery, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pa McGowan Institute for Regenerative Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA
    J Vasc Surg 59:1686-94. 2014
    ..The aims of this study were to characterize the temporal response of CD34+ progenitors following vascular injury in an ovine model and to evaluate an affinity pheresis approach to attenuate this response...
  23. doi request reprint Favoring the risk-benefit balance for upper extremity transplantation--the Pittsburgh Protocol
    Vijay S Gorantla
    Pittsburgh Reconstructive Transplantation Program, Division of Plastic Surgery, Department of Surgery, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    Hand Clin 27:511-20, ix-x. 2011
    ..Such therapies offer the promise of minimizing the risks, maximizing the benefits and optimizing outcomes of these innovative procedures...
  24. ncbi request reprint Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand
    P Toby H Coates
    Thomas E Starzl Transplantation Institute, Pittsburgh, PA, USA
    Blood 102:2513-21. 2003
    ..Both are mobilized into blood and lymphoid tissue by Flt3L, offering potential for their further characterization and possible therapeutic application...
  25. doi request reprint Effects of storage and leukoreduction on lymphocytes and Epstein-Barr virus genomes in platelet concentrates
    Lirong Qu
    Institute for Transfusion Medicine, 3636 Boulevard of the Allies, Pittsburgh, PA 15213, USA
    Transfusion 49:1580-3. 2009
    ..Epstein-Barr virus (EBV) persists in infected B lymphocytes in blood donors. Lymphocytes are viable during platelet (PLT) storage. The effects of storage and leukoreduction on lymphocytes and EBV genomes are evaluated...
  26. doi request reprint EBV-specific CD8+ T cells from asymptomatic pediatric thoracic transplant patients carrying chronic high EBV loads display contrasting features: activated phenotype and exhausted function
    Camila Macedo
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    J Immunol 186:5854-62. 2011
    ..Concomitant phenotypic and functional EBV monitoring is critical for identifying the complex "functional" versus "exhausted" signature of EBV-specific CD8(+) T cells, with implications for immunologic monitoring in the clinic...
  27. ncbi request reprint Rapid turnover of the CD8(+)CD28(-) T-cell subset of effector cells in the circulation of patients with head and neck cancer
    Takashi Tsukishiro
    University of Pittsburgh Cancer Institute, Research Pavilion at the Hillman Cancer Center, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Suite 1 27, Pittsburgh, PA 15213 1863, USA
    Cancer Immunol Immunother 52:599-607. 2003
    ..It appears that interactions of immune and tumor cells might determine the fate of this terminally differentiated effector cell subset...
  28. doi request reprint Real-time monitoring of non-viable airborne particles correlates with airborne colonies and represents an acceptable surrogate for daily assessment of cell-processing cleanroom performance
    Jay S Raval
    The Institute For Transfusion Medicine, Pittsburgh, PA, USA
    Cytotherapy 14:1144-50. 2012
    ..We present a procedure developed to monitor and interpret airborne particulates in an International Organization for Standardization (ISO) class 7 cleanroom used for the cell processing of Section 351 and Section 361 products...
  29. doi request reprint Regional anatomic and age effects on cell function of human adipose-derived stem cells
    Bret M Schipper
    Department of Surgery, Division of Plastic Surgery, University of Pittsburgh, PA 15213, USA
    Ann Plast Surg 60:538-44. 2008
    ..Additionally, we have shown age-related changes in function. These data will help select patients and cell harvest sites most suitable for tissue engineering therapies...
  30. ncbi request reprint Strategies for preclinical evaluation of dendritic cell subsets for promotion of transplant tolerance in the nonhuman primate
    P Toby H Coates
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, 200 Lathrop Street, Pittsburgh, PA 15213, USA
    Hum Immunol 63:955-65. 2002
    ....
  31. ncbi request reprint p53 as an immunotherapeutic target in head and neck cancer
    Thomas K Hoffmann
    Department of Otorhinolaryngology, Heinrich Heine University, Dusseldorf, Germany
    Adv Otorhinolaryngol 62:151-60. 2005
    ..It calls for modified approaches, in which altered peptide variants of the wt sequence p53 264-272 epitope are used in a vaccine in order to overcome unresponsiveness of T lymphocytes to the native epitope...
  32. ncbi request reprint Frequencies of tetramer+ T cells specific for the wild-type sequence p53(264-272) peptide in the circulation of patients with head and neck cancer
    Thomas K Hoffmann
    University of Pittsburgh Cancer Institute, University of Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 62:3521-9. 2002
    ..These findings emphasize the complexity of interactions between the tumor and the host immune system, and, thus, have particularly important implications for future p53-based immunization strategies...

Research Grants1

  1. Imaging Flow Cytometer
    Albert Donnenberg; Fiscal Year: 2006
    ..Should this proposal be funded, we are very well positioned to play a leading role in applying this new technology to cancer, immunology, and cell biology research. ..