Albert D Donnenberg
Affiliation: University of Pittsburgh
- Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodiesAlbert D Donnenberg
Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2 42 Research Pavilion, Pittsburgh, PA 15213, USA
Regen Med 6:701-6. 2011....
- Tumorigenic stem and progenitor cells: implications for the therapeutic index of anti-cancer agentsVera S Donnenberg
Hillman Cancer Center Research Pavilion, 5117 Centre Ave, Pittsburgh, PA 15213, United States
J Control Release 122:385-91. 2007..The therapeutic index of antineoplastics thus becomes one of sensitivity of cancer and normal stem cells, which are protected by the same mechanisms...
- Rare-event analysis in flow cytometryAlbert D Donnenberg
University of Pittsburgh School of Medicine, Hillman Cancer Research Center Suite2 42c, 5117 Center Avenue, Pittsburgh, PA 15213 2582, USA
Clin Lab Med 27:627-52, viii. 2007..A growing number of flow cytometry-based assays depend on rare-event detection for basic science and clinical applications...
- Intra-operative preparation of autologous bone marrow-derived CD34-enriched cellular products for cardiac therapyAlbert D Donnenberg
Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cytotherapy 13:441-8. 2011..In this report we present a methodology for intra-operative preparation of CD34(+) selected autologous bone marrow for autologous use in patients receiving coronary artery bypass grafts or left ventricular assist devices...
- Measurement of basal, substrate induced and total P-glycoprotein activity in bronchoalveolar lavage T-cell subsetsV S Donnenberg
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cytometry A 57:75-85. 2004..Our objectives were to evaluate P-gp activity in T cells freshly isolated from bronchoalveolar lavage (BAL) and to develop a strategy to distinguish between basal, in vitro substrate-induced, and total P-gp activities...
- Development and validation of a procedure to isolate viable bone marrow cells from the vertebrae of cadaveric organ donors for composite organ graftingVijay S Gorantla
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cytotherapy 14:104-13. 2012....
- Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolatesLudovic Zimmerlin
University of Pittsburgh Cancer Institute, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Tissue Eng Part A 17:93-106. 2011..Our data indicate that ASC enhance the growth of active, but not resting tumor cells. Thus, reconstructive therapy utilizing ASC-augmented whole fat should be postponed until there is no evidence of active disease...
- P-glycoprotein activity is decreased in CD4+ but not CD8+ lung allograft-infiltrating T cells during acute cellular rejectionVera S Donnenberg
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Transplantation 77:1699-706. 2004..Modulation of P-glycoprotein (P-gp) activity in graft-infiltrating T cells may alter their susceptibility to immunosuppression...
- Measurement of multiple drug resistance transporter activity in putative cancer stem/progenitor cellsVera S Donnenberg
Hillman Cancer Center, Pittsburgh, PA, USA
Methods Mol Biol 568:261-79. 2009....
- Adipogenic potential of adipose stem cell subpopulationsHan Li
Division of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
Plast Reconstr Surg 128:663-72. 2011..The aim of this study was to define distinct populations within the stromal vascular fraction based on surface marker expression, and to evaluate the ability of each cell type to differentiate to mature adipocytes...
- Rare event detection and analysis in flow cytometry: bone marrow mesenchymal stem cells, breast cancer stem/progenitor cells in malignant effusions, and pericytes in disaggregated adipose tissueLudovic Zimmerlin
Division of Hematology Oncology, Department of Medicine, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA
Methods Mol Biol 699:251-73. 2011....
- Localization of CD44 and CD90 positive cells to the invasive front of breast tumorsVera S Donnenberg
University of Pittsburgh Cancer Center, Pittsburgh, Pennsylvania, USA
Cytometry B Clin Cytom 78:287-301. 2010..A variety of markers have been proposed to identify breast cancer stem cells. Here, we used immunohistostaining and flow cytometry to analyze their interrelationships and to sort cells for tumorigenicity studies...
- Stromal vascular progenitors in adult human adipose tissueLudovic Zimmerlin
Department of Medicine, Division of Hematology Oncology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
Cytometry A 77:22-30. 2010..A CD146+/CD34+ subset detected by flow cytometry at low frequency suggests a population transitional between pericytes and SA-ASC...
- Identification, rare-event detection and analysis of dendritic cell subsets in broncho-alveolar lavage fluid and peripheral blood by flow cytometryVera S Donnenberg
University of Pittsburgh Cancer Institute, Department of Surgery, The Hillman Cancer Center, 5117 Centre Avenue, Pittsburgh PA 15213, USA
Front Biosci 8:s1175-80. 2003....
- Human adipose stromal vascular cell delivery in a fibrin sprayLudovic Zimmerlin
Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Cytotherapy 15:102-8. 2013..We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system...
- Multiple drug resistance in cancer revisited: the cancer stem cell hypothesisVera S Donnenberg
Department of Surgery, Division of Thoracic Surgery, University of Pittsburgh Cancer Institute, Pennsilvania, USA
J Clin Pharmacol 45:872-7. 2005..In the context of this hypothesis, the authors review the discovery of MDR transporters in cancer and normal stem cells and the failure of MDR reversal agents to increase the therapeutic index of substrate antineoplastic agents...
- Regenerative therapy after cancer: what are the risks?Vera S Donnenberg
University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
Tissue Eng Part B Rev 16:567-75. 2010....
- Flow cytometric determination of stem/progenitor content in epithelial tissues: An example from nonsmall lung cancer and normal lungVera S Donnenberg
Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA USA University of Pittsburgh Cancer Center, Pittsburgh, PA USA McGowan Institute of Regenerative Medicine, Pittsburgh PA USA
Cytometry A 83:141-9. 2013..The results of bivariate analysis show a striking similarity between the expression of stem/progenitor markers on lung tumor and adjacent tumor-free lung. © 2012 International Society for Advancement of Cytometry...
- KIT (CD117) Expression in a Subset of Non-Small Cell Lung Carcinoma (NSCLC) PatientsAlbert D Donnenberg
Hillman Cancer Center, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
PLoS ONE 7:e52885. 2012..We conclude that NSCLC tumors divide into CD117(low) and CD117(high). Overexpression of CD117 in CD117(high) NSCLC supports exploring KIT as a therapeutic target in this subset of patients...
- Favoring the risk-benefit balance for upper extremity transplantation--the Pittsburgh ProtocolVijay S Gorantla
Pittsburgh Reconstructive Transplantation Program, Division of Plastic Surgery, Department of Surgery, 3550 Terrace Street, Pittsburgh, PA 15261, USA
Hand Clin 27:511-20, ix-x. 2011..Such therapies offer the promise of minimizing the risks, maximizing the benefits and optimizing outcomes of these innovative procedures...
- Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligandP Toby H Coates
Thomas E. Starzl Transplantation Institute, Pittsburgh, PA, USA
Blood 102:2513-21. 2003..Both are mobilized into blood and lymphoid tissue by Flt3L, offering potential for their further characterization and possible therapeutic application...
- Effects of storage and leukoreduction on lymphocytes and Epstein-Barr virus genomes in platelet concentratesLirong Qu
Institute for Transfusion Medicine, 3636 Boulevard of the Allies, Pittsburgh, PA 15213, USA
Transfusion 49:1580-3. 2009..Epstein-Barr virus (EBV) persists in infected B lymphocytes in blood donors. Lymphocytes are viable during platelet (PLT) storage. The effects of storage and leukoreduction on lymphocytes and EBV genomes are evaluated...
- EBV-specific CD8+ T cells from asymptomatic pediatric thoracic transplant patients carrying chronic high EBV loads display contrasting features: activated phenotype and exhausted functionCamila Macedo
Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
J Immunol 186:5854-62. 2011....
- Rapid turnover of the CD8(+)CD28(-) T-cell subset of effector cells in the circulation of patients with head and neck cancerTakashi Tsukishiro
University of Pittsburgh Cancer Institute, Research Pavilion at the Hillman Cancer Center, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Suite 1.27, Pittsburgh, PA 15213-1863, USA
Cancer Immunol Immunother 52:599-607. 2003..It appears that interactions of immune and tumor cells might determine the fate of this terminally differentiated effector cell subset...
- Regional anatomic and age effects on cell function of human adipose-derived stem cellsBret M Schipper
Department of Surgery, Division of Plastic Surgery, University of Pittsburgh, PA 15213, USA
Ann Plast Surg 60:538-44. 2008..Additionally, we have shown age-related changes in function. These data will help select patients and cell harvest sites most suitable for tissue engineering therapies...
- Strategies for preclinical evaluation of dendritic cell subsets for promotion of transplant tolerance in the nonhuman primateP Toby H Coates
Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, 200 Lathrop Street, Pittsburgh, PA 15213, USA
Hum Immunol 63:955-65. 2002....
- p53 as an immunotherapeutic target in head and neck cancerThomas K Hoffmann
Department of Otorhinolaryngology, Heinrich Heine University, Dusseldorf, Germany
Adv Otorhinolaryngol 62:151-60. 2005..It calls for modified approaches, in which altered peptide variants of the wt sequence p53 264-272 epitope are used in a vaccine in order to overcome unresponsiveness of T lymphocytes to the native epitope...
- Frequencies of tetramer+ T cells specific for the wild-type sequence p53(264-272) peptide in the circulation of patients with head and neck cancerThomas K Hoffmann
University of Pittsburgh Cancer Institute, University of Pittsburgh, Pennsylvania 15213, USA
Cancer Res 62:3521-9. 2002..These findings emphasize the complexity of interactions between the tumor and the host immune system, and, thus, have particularly important implications for future p53-based immunization strategies...
- Imaging Flow CytometerAlbert Donnenberg; Fiscal Year: 2006..Should this proposal be funded, we are very well positioned to play a leading role in applying this new technology to cancer, immunology, and cell biology research. ..