Research Topics
Genomes and Genes | Roberto DominguezSummaryAffiliation: University of Pennsylvania Country: USA Publications
Research Grants
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Publications
Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding modelsEmma Borrego-Diaz
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
J Struct Biol 155:230-8. 2006....
Structural insights into de novo actin polymerizationRoberto Dominguez
Department of Physiology, 3700 Hamilton Walk, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
Curr Opin Struct Biol 20:217-25. 2010..Filament nucleation and elongation factors frequently interact with Rho-family GTPases, which relay signals from membrane receptors to regulate actin cytoskeleton remodeling...- The beta-thymosin/WH2 fold: multifunctionality and structureRoberto Dominguez
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104, USA
Ann N Y Acad Sci 1112:86-94. 2007.... - Actin structure and functionRoberto Dominguez
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
Annu Rev Biophys 40:169-86. 2011..Here we review structures of G-actin and F-actin and discuss some of the interactions that control the polymerization and disassembly of actin... - Actin filament nucleation and elongation factors--structure-function relationshipsRoberto Dominguez
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
Crit Rev Biochem Mol Biol 44:351-66. 2009..In contrast, the elongation function among W-based nucleators has been "outsourced" to a dedicated family of proteins, Eva/VASP, which are related to WASP-family NPFs... - Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASPFrancois Ferron
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
EMBO J 26:4597-606. 2007..The actin monomer bound at the GAB domain is proposed to be in position to join the barbed end of the growing filament concurrently with the release of profilin... - Crystal structure of the actin-binding domain of alpha-actinin-4 Lys255Glu mutant implicated in focal segmental glomerulosclerosisSung Haeng Lee
University of Pennsylvania School of Medicine, Department of Physiology, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
J Mol Biol 376:317-24. 2008..The absence of a structural change suggests that the compact ABD conformation observed in the majority of the structures is highly stable and is preserved in solution, even in FSGS mutant ABDs... - Mechanism of actin filament nucleation by Vibrio VopL and implications for tandem W domain nucleationSuk Namgoong
Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
Nat Struct Mol Biol 18:1060-7. 2011..Combined, the results suggest that VopL stabilizes a hexameric double-stranded pointed end nucleus. Analysis of hybrid constructs of VopL and the eukaryotic nucleator Spire suggest that Spire may also function as a dimer in cells... - Structure-function study of the N-terminal domain of exocyst subunit Sec3Kyuwon Baek
Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 285:10424-33. 2010..Through its ability to bind the small GTPase Cdc42 and phospholipids, the PH domain of Sec3 functions as a coincidence detector at the plasma membrane... - Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin bindingKyuwon Baek
Department of Physiology, 3700 Hamilton Walk, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
Proc Natl Acad Sci U S A 105:11748-53. 2008..As a result, the nucleotide cleft becomes moderately more open in the profilin-actin complex, probably explaining the stimulation of nucleotide exchange on actin by profilin... - X-ray scattering study of actin polymerization nuclei assembled by tandem W domainsGrzegorz Rebowski
Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 105:10785-90. 2008..The successful stabilization of filament-like multiactin assemblies opens the way to the crystallographic investigation of intersubunit contacts in the actin filament... - Structure of a longitudinal actin dimer assembled by tandem w domains: implications for actin filament nucleationGrzegorz Rebowski
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
J Mol Biol 403:11-23. 2010..We finally show that the simple addition of the C-terminal pointed end-capping helix of thymosin β4 to tandem W domains can change their activity from actin filament nucleation to monomer sequestration... - Mechanism of actin filament bundling by fascinSilvia Jansen
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 286:30087-96. 2011..These molecules could guide the rational design of new anticancer fascin inhibitors... - X-ray scattering study of activated Arp2/3 complex with bound actin-WCAMalgorzata Boczkowska
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, A507 Richards Building, Philadelphia, PA 19104 6085, USA
Structure 16:695-704. 2008..The results support a model of activation that is consistent with most of the biochemical observations... - Large nucleotide-dependent conformational change in Rab28Sung Haeng Lee
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, A507 Richards Building, Philadelphia, PA 19104 6085, USA
FEBS Lett 582:4107-11. 2008..The double-glycine motif, which is conserved in the Arf family, only occurs in Rab28 and Rab7B of the Rab family, and may have a profound effect on their catalytic activities... - Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimerSung Haeng Lee
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
Proc Natl Acad Sci U S A 104:16122-7. 2007..This effect is linked to the formation of the antiparallel actin dimer because a construct lacking helices 1 and 2 binds only one actin and inhibits nucleotide exchange less potently... - Structural basis for the actin-binding function of missing-in-metastasisSung Haeng Lee
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104, USA
Structure 15:145-55. 2007..The WH2 of MIM is longer than the prototypical WH2, interacting with all four subdomains of actin. We characterize a similar WH2 at the C terminus of IRSp53 and propose that in these two proteins WH2 performs a scaffolding function... - Mechanisms of plasma membrane targeting of formin mDia2 through its amino terminal domainsRoman Gorelik
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
Mol Biol Cell 22:189-201. 2011..These results suggest that the entire N terminus of mDia2 serves as a coincidence detection module, directing mDia2 to the plasma membrane through interactions with phospholipids and activated Rif... - Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamicsPhilip Graceffa
Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA
J Biol Chem 278:34172-80. 2003..An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state... - Solution properties of TMR-actin: when biochemical and crystal data agreeRoberto Dominguez
Biophys J 85:2073-4. 2003 - Actin-binding proteins--a unifying hypothesisRoberto Dominguez
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Trends Biochem Sci 29:572-8. 2004..As the pocket remains accessible in F-actin, it is proposed that this pocket represents a primary target for F-actin-binding proteins, such as calponin-homology-related proteins and myosin... - Crystal structures of the vitamin D-binding protein and its complex with actin: structural basis of the actin-scavenger systemLudovic R Otterbein
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 99:8003-8. 2002..Formation of the strong actin-DBP complex proceeds with limited conformational changes to both proteins, demonstrating how DBP has evolved to become an effective actin-scavenger protein... - Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin VMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, Massachusetts 02472, USA
Acta Crystallogr D Biol Crystallogr 58:1882-5. 2002..The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model... - Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implicationsMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
EMBO J 22:362-71. 2003..The resulting 'free' N-lobes of myosin light chains in the extended conformation could mediate the formation of ternary complexes during protein localization and/or partner recruitment... - Structural basis of protein phosphatase 1 regulationMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, Massachusetts 02472, USA
Nature 429:780-4. 2004..The structure has general implications for the control of PP1 activity by other regulatory subunits... - Structure of the light chain-binding domain of myosin VMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 102:12718-23. 2005..The model points to a prominent role of the LCBD in the function, regulation, and molecular interactions of myosin V... - Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assemblyDavid Chereau
Boston Biomedical Research Institute, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 102:16644-9. 2005..The structures also suggest a mechanism for how profilin-binding Pro-rich sequences positioned N-terminal to WH2 could feed actin monomers directly to WH2, thereby playing a role in filament elongation... - Understanding the role of the G-actin-binding domain of Ena/VASP in actin assemblyDavid Chereau
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
J Struct Biol 155:195-201. 2006..Conserved domain architecture suggests that WASP may work similarly... - Leiomodin is an actin filament nucleator in muscle cellsDavid Chereau
Boston Biomedical Research Institute, Watertown, MA 02472, USA
Science 320:239-43. 2008..Knocking down leiomodin severely compromised sarcomere assembly in cultured muscle cells, which suggests a role for leiomodin in the nucleation of tropomyosin-decorated filaments in muscles... - Cortactin binding to F-actin revealed by electron microscopy and 3D reconstructionKiran Pant
Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
J Mol Biol 359:840-7. 2006..In addition, cortactin may act as a structural adapter protein, stabilizing nascent filament branches while mediating the simultaneous recruitment of Arp2/3 and WASP... - Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolutionLudovic R Otterbein
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Structure 10:557-67. 2002..The structures have important implications for our understanding of target binding and cooperativity of Ca(2+) binding in the S100 family...
Research Grants
- Structure of the Smooth Muscle Myosin PhosphataseRoberto Dominguez; Fiscal Year: 2007..This knowledge will prove invaluable for the development of new drugs to treat cardiovascular diseases such as hypertension. ..
- Structural Basis of Actin Cytoskeleton DynamicsRoberto Dominguez; Fiscal Year: 2010..The knowledge gained here will be of great relevance to understanding the molecular mechanisms of disease. ..
- Structural Basis of Actin Cytoskeleton DynamicsRoberto Dominguez; Fiscal Year: 2009..By studying these motifs in parallel we will establish common and distinctive features of their structures and interactions with G- and F-actin that determine their functions in the regulation of the cytoskeleton. ..
- ATOMIC STRUCTURE OF SMOOTH MUSCLE CALDESMONRoberto Dominguez; Fiscal Year: 2004....
- BAR proteins linking membrane and cytoskeleton dynamicsRoberto Dominguez; Fiscal Year: 2010..PICK1, in particular, is a recognized drug target, and has been implicated in long-term depression, such that knowledge of it structure may have a potential impact on human health. ..