Research Topics
Genomes and GenesSpecies | Yohei DoiSummaryAffiliation: University of Pittsburgh Country: USA Publications
| Collaborators
|
Detail Information
Publications
Genetic environment of 16S rRNA methylase gene rmtDYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Falk Medical Building Suite 3A, 3601 Fifth Avenue, Pittsburgh, PA 15213, USA
Antimicrob Agents Chemother 52:2270-2. 2008..rmtD found in Klebsiella pneumoniae appeared to have been mobilized from P. aeruginosa by an IS26-mediated event...- Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compoundYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
J Clin Microbiol 46:4083-6. 2008..When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type beta-lactamases from those producing other types of beta-lactamases... - Genetic basis of multidrug resistance in Acinetobacter baumannii clinical isolates at a tertiary medical center in PennsylvaniaJennifer M Adams-Haduch
Division of Infectious Diseases, University of Pittsburgh Medical Center, S829 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261
Antimicrob Agents Chemother 52:3837-43. 2008..Fifty-nine percent of the MDR isolates belonged to a single clonal group over the course of the study period, as demonstrated by pulsed-field gel electrophoresis... - Identification of 16S rRNA methylase-producing Acinetobacter baumannii clinical strains in North AmericaYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
Antimicrob Agents Chemother 51:4209-10. 2007..Two of the isolates coproduced OXA-23 beta-lactamase and were highly resistant to carbapenems as well. The isolates were genetically closely related by pulsed-field gel electrophoresis... - High prevalence of metallo-beta-lactamase and 16S rRNA methylase coproduction among imipenem-resistant Pseudomonas aeruginosa isolates in BrazilYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Falk Medical Building Suite 3A, 3601 Fifth Avenue, Pittsburgh PA 15213, USA
Antimicrob Agents Chemother 51:3388-90. 2007..Of them, 57% and 75% produced SPM-1 and RmtD, respectively. Of note, 51% produced both enzymes, suggesting that their coproduction is already common in this geographic area... - Coproduction of novel 16S rRNA methylase RmtD and metallo-beta-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from BrazilYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Falk Medical Building Suite 3A, 3601 Fifth Avenue, Pittsburgh, PA 15213, USA
Antimicrob Agents Chemother 51:852-6. 2007..This is also the first report to document coproduction of a metallo-beta-lactamase and a 16S rRNA methylase, a combination that would severely compromise therapeutic options for the infected patients... 
16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosidesYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
Clin Infect Dis 45:88-94. 2007..A 2-tiered approach, consisting of disk diffusion tests followed by confirmation with polymerase chain reaction, is recommended for detection of 16S rRNA methylase-mediated resistance...
Interspecies spread of Klebsiella pneumoniae carbapenemase gene in a single patientHanna E Sidjabat
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
Clin Infect Dis 49:1736-8. 2009..Molecular typing and plasmid analysis suggested that the KPC gene was acquired by E. coli, most likely from K. pneumoniae, and was subsequently transferred to S. marcescens...- Features of infections due to Klebsiella pneumoniae carbapenemase-producing Escherichia coli: emergence of sequence type 131Young Ah Kim
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
Clin Infect Dis 55:224-31. 2012..Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae has become endemic in many US hospitals. On the other hand, KPC-producing Escherichia coli remains rare... - The role of horizontal gene transfer in the dissemination of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in an endemic settingYohei Doi
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Diagn Microbiol Infect Dis 74:34-8. 2012..HGT among clonally distinct strains contributes to the transmission dynamics of these ESBL-producing Gram-negative bacteria and should be considered when evaluating the spread of these pathogens... - Treatment outcome of bacteremia due to KPC-producing Klebsiella pneumoniae: superiority of combination antimicrobial regimensZubair A Qureshi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Antimicrob Agents Chemother 56:2108-13. 2012..7% (8/12). The use of combination therapy for definitive therapy appears to be associated with improved survival in bacteremia due to KPC-producing K. pneumoniae... - Clinical features and molecular epidemiology of CMY-type beta-lactamase-producing Escherichia coliHanna E Sidjabat
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
Clin Infect Dis 48:739-44. 2009..Of the several groups of plasmid-mediated AmpC beta-lactamases, CMY-type beta-lactamase is the most common in the United States... - Clinical characteristics of bloodstream infections due to ampicillin-sulbactam-resistant, non-extended- spectrum-beta-lactamase-producing Escherichia coli and the role of TEM-1 hyperproductionRebecca I Waltner-Toews
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
Antimicrob Agents Chemother 55:495-501. 2011..These results suggest high-level bla(TEM-1) expression as the predominant cause of ampicillin-sulbactam resistance and also the presence of yet-unidentified factors promoting overexpression of bla(TEM-1) in these isolates... - Colistin-resistant, Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae belonging to the international epidemic clone ST258Tatiana Bogdanovich
Division of Infectious Diseases, Department of Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Clin Infect Dis 53:373-6. 2011..These cases likely represented both emergence of resistance and transmission of resistant organism. The colistin-resistant isolates were able to persist in the absence of selective pressure in vitro... - Molecular epidemiology of carbapenem-nonsusceptible Acinetobacter baumannii in the United StatesJennifer M Adams-Haduch
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
J Clin Microbiol 49:3849-54. 2011..S. hospitals constitute part of the global epidemic driven by CC92, but have unique STs other than ST92, which may be spreading by means of patient transfer between health care facilities within the United States... - Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western PennsylvaniaHanna E Sidjabat
Division of Infectious Diseases, University of Pittsburgh Medical Center, 3550 Terrace Street, Pittsburgh, PA 15261, USA
Antimicrob Agents Chemother 53:4733-9. 2009..Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States... - Identification of diverse OXA-40 group carbapenemases, including a novel variant, OXA-160, from Acinetobacter baumannii in PennsylvaniaGuo Bao Tian
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
Antimicrob Agents Chemother 55:429-32. 2011..The genetic environment of bla(OXA-160) and bla(OXA-40) beyond the putative XerC/XerD recombination sites was distinct from the scaffold reported previously... - Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepimeGuo Bao Tian
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Scaife Hall S829, 3550 Terrace Street, Pittsburgh, PA 15261, USA
Antimicrob Agents Chemother 55:4922-5. 2011..This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover... - Reduced susceptibility to cefepime among Escherichia coli clinical isolates producing novel variants of CMY-2 beta-lactamaseYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, 3550 Terrace Street, Pittsburgh, PA 15261, USA
Antimicrob Agents Chemother 53:3159-61. 2009..Sequencing of the bla(CMY) genes revealed two novel variants (CMY-33 and -44) with two- to four-amino-acid deletions in the H-10 helix. The deletions were responsible for 12- to 24-fold increases in the MICs of cefepime... - Clinical and microbiologic characteristics of cephalosporin-resistant Escherichia coli at three centers in the United StatesYoon Soo Park
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Antimicrob Agents Chemother 56:1870-6. 2012..2% being susceptible due to the predominance of CTX-M-15. These findings have implications in the selection of appropriate empirical therapy when infection due to cephalosporin-resistant E. coli is suspected... - Detection of plasmid-mediated class C beta-lactamasesYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Falk Medical Building Suite 3A, 3601 Fifth Avenue, Pittsburgh, PA 15213, USA
Int J Infect Dis 11:191-7. 2007.... - Community-Associated Extended-Spectrum β-Lactamase-Producing Escherichia coli Infection in the United StatesYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pennsylvania
Clin Infect Dis 56:641-8. 2013..This epidemiologic shift has implications for the empiric management of community-associated infection when involvement of E. coli is suspected... - PME-1, an extended-spectrum β-lactamase identified in Pseudomonas aeruginosaGuo Bao Tian
Division of Infectious Diseases, University of Pittsburgh School of Medicine, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Antimicrob Agents Chemother 55:2710-3. 2011..PME-1 was inhibited well by β-lactamase inhibitors, including clavulanic acid, sulbactam, and tazobactam. The bla(PME-1) gene was carried by an approximately 9-kb plasmid and flanked by tandem ISCR24 elements... - High mortality rates among solid organ transplant recipients infected with extensively drug-resistant Acinetobacter baumannii: using in vitro antibiotic combination testing to identify the combination of a carbapenem and colistin as an effective treatmentRyan K Shields
University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA
Diagn Microbiol Infect Dis 70:246-52. 2011..Subsequently, 80% (4/5) of transplant patients were treated successfully with carbepenem-colistin regimens... - Screening for Acinetobacter baumannii colonization by use of spongesYohei Doi
Division of Infectious Diseases, University of Pittsburgh Medical Center, Scaife Hall S829, 3550 Terrace Street, Pittsburgh, PA 15261, USA
J Clin Microbiol 49:154-8. 2011..003). The combined sensitivity for the upper arm and leg with a sponge was 89.1%. The novel screening method using sterile sponges was easy to perform and achieved excellent sensitivity for the detection of A. baumannii colonization... - Risk factors and outcome of extended-spectrum β-lactamase-producing Enterobacter cloacae bloodstream infectionsZubair A Qureshi
Division of Infectious Diseases, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA
Int J Antimicrob Agents 37:26-32. 2011..The majority of isolates produced SHV-type ESBL, whereas two isolates produced CTX-M-type ESBL. Initial therapy with a carbapenem appears to be associated with improved clinical outcome in BSI due to ESBL-producing E. cloacae... - Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacaeDora Szabo
Division of Infectious Diseases, University of Pittsburgh Medical Center, PA 15213, USA
Antimicrob Agents Chemother 50:2833-5. 2006..Gene transcription of OmpD and OmpF was diminished in the ertapenem-resistant isolate. An efflux pump inhibitor decreased the MICs of ertapenem in the resistant strain, suggesting a potential role of efflux pumps in ertapenem resistance... - [Antimicrobial resistance testing in clinical practice]Yohei Doi
Division of Infectious Diseases, University of Pittsburgh School of Medicine, USA
Nihon Rinsho 70:272-5. 2012..Antimicrobial resistance testing plays a crucial role in patient management, infection control and monitoring of local as well as national and international epidemiology... - Spread of novel aminoglycoside resistance gene aac(6')-Iad among Acinetobacter clinical isolates in JapanYohei Doi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
Antimicrob Agents Chemother 48:2075-80. 2004..These results suggest that aac(6')-Iad plays a substantial role in amikacin resistance among Acinetobacter spp. in Japan... - Genetic environments of the rmtA gene in Pseudomonas aeruginosa clinical isolatesKunikazu Yamane
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashi Murayama, Tokyo 208 0011, Japan
Antimicrob Agents Chemother 48:2069-74. 2004.... - Nosocomial spread of ceftazidime-resistant Klebsiella pneumoniae strains producing a novel class a beta-lactamase, GES-3, in a neonatal intensive care unit in JapanJun ichi Wachino
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashi Murayama, Tokyo 208 0011, Japan
Antimicrob Agents Chemother 48:1960-7. 2004..These findings suggest that within the NICU setting genetically related K. pneumoniae strains carrying the bla(GES) gene were ambushed with genetic rearrangements that caused the multiplication and translocation of the bla(GES) gene... - Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosidesYohei Doi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 48:491-6. 2004..aeruginosa in Japan. This is a cause for concern since other treatment options are often limited in patients requiring highly potent aminoglycosides such as amikacin and tobramycin... - Characterization of Klebsiella pneumoniae and Escherichia coli strains that produce CTX-M-2-type broad spectrum beta-lactamase isolated from a child with leukemiaToshiya Ohkawa
Department of Pediatrics, Faculty of Medicine, Kagoshima University, Japan
Pediatr Infect Dis J 21:260-2. 2002..Patients with frequent episodes of neutropenia and prophylactic administration of beta-lactams are at risk of harboring colonizing strains that produce broad spectrum beta-lactamases... - [IMP-1 type metalo-beta-lactamase producing Serratia marcescens strains isolated from blood culture between 1991 to 2000]Tatsuya Nakamura
Clinical Central Laboratory, Kansai Medical University Hospital
Kansenshogaku Zasshi 76:246-53. 2002..It cannot be excluded that the resistant bacteria spread as the amount of carbapenem consumption increased. Prudent use of antibiotics is mandatory to prevent further dissemination of such MBL producers... - Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosaKeiko Yokoyama
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
Lancet 362:1888-93. 2003..Further dissemination of the rmtA gene in nosocomial bacteria could be a matter of concern in the future... - Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolateYohei Doi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gauken, Musashi Murayama, Tokyo 208 0011, Japan
Antimicrob Agents Chemother 46:2427-34. 2002..bla(CMY-9) was closely located downstream of the end of the common region. These observations are indicative of the exogenous derivation of bla(CMY-9) from some environmental microorganisms such as aeromonads... - Inhibitor-sensitive AmpC beta-lactamase variant produced by an Escherichia coli clinical isolate resistant to oxyiminocephalosporins and cephamycinsYohei Doi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashi Murayama, Tokyo 208 0011, Japan
Antimicrob Agents Chemother 48:2652-8. 2004..To our knowledge, this is the first report to have characterized an E. coli ampC that encodes chromosomal AmpC beta-lactamase sensitive to the available beta-lactamase inhibitors... - Molecular characterization of a cephamycin-hydrolyzing and inhibitor-resistant class A beta-lactamase, GES-4, possessing a single G170S substitution in the omega-loopJun ichi Wachino
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashi Murayama, Tokyo, Japan
Antimicrob Agents Chemother 48:2905-10. 2004.... - 16S ribosomal RNA methylase RmtD produced by Klebsiella pneumoniae in BrazilKunikazu Yamane
J Antimicrob Chemother 61:746-7. 2008 - Escherichia coli isolate coproducing 16S rRNA Methylase and CTX-M-type extended-spectrum beta-lactamase isolated from an outpatient in the United StatesYohei Doi
Antimicrob Agents Chemother 52:1204-5. 2008 - Multiclonal outbreak of Klebsiella pneumoniae producing extended-spectrum beta-lactamase CTX-M-2 and novel variant CTX-M-59 in a neonatal intensive care unit in BrazilDoroti de Oliveira Garcia
Instituto Adolfo Lutz, Sao Paulo, Brazil
Antimicrob Agents Chemother 52:1790-3. 2008..Of the 10 pulsotypes identified during the outbreak and follow-up periods, nine produced CTX-M-2 or its new variant CTX-M-59 and one produced SHV-5. bla(CTX-M-2/59) genes were located on closely related plasmids that were transferable... - High prevalence of CTX-M-15-producing Klebsiella pneumoniae among inpatients and outpatients with urinary tract infection in Southern IndiaYohei Doi
J Antimicrob Chemother 61:1393-4. 2008 - Characterization of a naturally occurring class D beta-lactamase from Achromobacter xylosoxidansYohei Doi
Service de Bacteriologie Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculté deMédecine Paris Sud, Universite Paris XI, 94275 K Bicêtre, France
Antimicrob Agents Chemother 52:1952-6. 2008..xylosoxidans strains tested (n = 5), indicating that this beta-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of bla(OXA-114) expression... - Nomenclature of plasmid-mediated 16S rRNA methylases responsible for panaminoglycoside resistanceYohei Doi
Antimicrob Agents Chemother 52:2287-8. 2008 - Community-acquired extended-spectrum beta-lactamase producers, United StatesYohei Doi
Emerg Infect Dis 13:1121-3. 2007 - Global spread of multiple aminoglycoside resistance genesKunikazu Yamane
National Institute of Infectious Diseases, Tokyo, Japan
Emerg Infect Dis 11:951-3. 2005..ArmA, which had been identified exclusively in Europe, was also found in several gram-negative pathogenic bacilli isolated in Japan, suggesting global dissemination of hazardous multiple aminoglycoside resistance genes... - Practical methods using boronic acid compounds for identification of class C beta-lactamase-producing Klebsiella pneumoniae and Escherichia coliTetsuya Yagi
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
J Clin Microbiol 43:2551-8. 2005..These methods will provide useful information needed for targeted antimicrobial therapy and better infection control... - Novel plasmid-mediated 16S rRNA methylase, RmtC, found in a proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosidesJun-ichi Wachino
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan
Antimicrob Agents Chemother 50:178-84. 2006..The acceleration of aminoglycoside resistance among gram-negative bacilli by producing plasmid-mediated 16S rRNA methylases, such as RmtC, RmtB, and RmtA, may indeed become an actual clinical hazard in the near future... - PCR classification of CTX-M-type beta-lactamase genes identified in clinically isolated gram-negative bacilli in JapanNaohiro Shibata
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 50:791-5. 2006..Fifty-seven, 161, and 99 strains harbored bla(CTX-M) genes belonging to the bla(CTX-M-1), bla(CTX-M-2), and bla(CTX-M-9) clusters, respectively... - A step closer to extreme drug resistance (XDR) in gram-negative bacilliDavid L Paterson
Clin Infect Dis 45:1179-81. 2007 - A novel apoptosis-inducing protein from Helicobacter pyloriKeigo Shibayama
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
Mol Microbiol 47:443-51. 2003..pylori-mediated apoptosis. Our findings provide new insights into H. pylori pathogenicity and reveal a novel aspect of the bacterial gamma-glutamyl transpeptidase function... - A new TEM-derived extended-spectrum beta-lactamase (TEM-91) with an R164C substitution at the omega-loop confers ceftazidime resistanceHiroshi Kurokawa
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan
Antimicrob Agents Chemother 47:2981-3. 2003..The isoelectric point (pI), K(m), and k(cat) of TEM-91 for ceftazidime were 5.7, 179 microM, and 29.0 s(-1), respectively. The K(i) of clavulanic acid for ceftazidime hydrolysis was 30.3 nM... - PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integronNaohiro Shibata
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan
J Clin Microbiol 41:5407-13. 2003..In the present study, it was confirmed that strains carrying bla(IMP-1) with a class 1 integron are the most prevalent type in Japan, although several intI3 carriers have also been identified sporadically in this country... - Escherichia coli producing CTX-M-2 beta-lactamase in cattle, JapanYutaka Shiraki
Gifu Prefectural Office of Meat Inspection, Gifu, Japan
Emerg Infect Dis 10:69-75. 2004..coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large... - KPC type beta-lactamase, rural PennsylvaniaJonathan Pope
Emerg Infect Dis 12:1613-4. 2006