Henrik G Dohlman

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Regulation of cell signaling dynamics by the protein kinase-scaffold Ste5
    Nan Hao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 30:649-56. 2008
  2. ncbi request reprint G proteins and pheromone signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Annu Rev Physiol 64:129-52. 2002
  3. ncbi request reprint Pheromone signaling pathways in yeast
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building Room 430, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci STKE 2006:cm6. 2006
  4. doi request reprint A scaffold makes the switch
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci Signal 1:pe46. 2008
  5. pmc Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae
    Scott A Chasse
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Eukaryot Cell 5:330-46. 2006
  6. pmc Persistent activation by constitutive Ste7 promotes Kss1-mediated invasive growth but fails to support Fus3-dependent mating in yeast
    Seth Maleri
    Department of Biochemistry and Biophysics, CB 7260, 512 ME Jones, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Mol Cell Biol 24:9221-38. 2004
  7. pmc Systematic analysis of essential genes reveals important regulators of G protein signaling
    Steven D Cappell
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 38:746-57. 2010
  8. pmc Combined computational and experimental analysis reveals mitogen-activated protein kinase-mediated feedback phosphorylation as a mechanism for signaling specificity
    Nan Hao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 23:3899-910. 2012
  9. pmc Control of MAPK specificity by feedback phosphorylation of shared adaptor protein Ste50
    Nan Hao
    Department of Biochemistry, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 283:33798-802. 2008
  10. pmc Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity
    Jillian H Hurst
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 288:18660-71. 2013

Collaborators

Detail Information

Publications55

  1. pmc Regulation of cell signaling dynamics by the protein kinase-scaffold Ste5
    Nan Hao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 30:649-56. 2008
    ..We propose that scaffold proteins serve to modulate the temporal and dose-response behavior of the MAP kinase...
  2. ncbi request reprint G proteins and pheromone signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Annu Rev Physiol 64:129-52. 2002
    ....
  3. ncbi request reprint Pheromone signaling pathways in yeast
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building Room 430, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci STKE 2006:cm6. 2006
    ..This updated Connections Map in the Database of Cell Signaling includes several major revisions to this prototypical signal response pathway...
  4. doi request reprint A scaffold makes the switch
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci Signal 1:pe46. 2008
    ..New findings reveal that the graded-to-binary conversion can be turned on or off, depending on the location of the scaffold within the cell...
  5. pmc Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae
    Scott A Chasse
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Eukaryot Cell 5:330-46. 2006
    ..These findings suggest that Sst2 is the principal regulator of Gpa1-mediated signaling in vivo but that other proteins also contribute in distinct ways to pathway regulation...
  6. pmc Persistent activation by constitutive Ste7 promotes Kss1-mediated invasive growth but fails to support Fus3-dependent mating in yeast
    Seth Maleri
    Department of Biochemistry and Biophysics, CB 7260, 512 ME Jones, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Mol Cell Biol 24:9221-38. 2004
    ..This scaffold-independent activation of Kss1 by constitutive Ste7 and the existence of mechanisms for pathway-specific promoter discrimination impose a unique developmental fate independently of any distinguishing external stimuli...
  7. pmc Systematic analysis of essential genes reveals important regulators of G protein signaling
    Steven D Cappell
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 38:746-57. 2010
    ..These insights to the G protein signaling network reveal the essential genome as an untapped resource for identifying new components and regulators of signal transduction pathways...
  8. pmc Combined computational and experimental analysis reveals mitogen-activated protein kinase-mediated feedback phosphorylation as a mechanism for signaling specificity
    Nan Hao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 23:3899-910. 2012
    ..These findings reveal how feedback phosphorylation of a common pathway component can limit the activity of a competing MAP kinase through feedback phosphorylation of a common activator, and thereby promote signal fidelity...
  9. pmc Control of MAPK specificity by feedback phosphorylation of shared adaptor protein Ste50
    Nan Hao
    Department of Biochemistry, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 283:33798-802. 2008
    ..Thus feedback regulation of a shared component can restrict the activity of a competing MAP kinase to ensure signal fidelity...
  10. pmc Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity
    Jillian H Hurst
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 288:18660-71. 2013
    ..These results reveal a feedback loop wherein one MAPK limits the ubiquitination of an upstream MAPKK and thereby prevents spurious activation of a second competing MAPK. ..
  11. ncbi request reprint The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:25168-76. 2007
    ..Our findings reveal the existence of an unusual Gbeta subunit, one having multiple functions within the cell in addition to serving as a signal transducer for cell surface receptors and intracellular effectors...
  12. ncbi request reprint A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway
    Nan Hao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Curr Biol 17:659-67. 2007
    ..For instance in yeast, the mitogen-activated protein (MAP) kinase Hog1 is activated and inactivated within minutes, even when the osmotic-stress stimulus is sustained...
  13. ncbi request reprint Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Biol Chem 281:1964-9. 2006
    ..In the absence of sumoylation Tec1 is more rapidly degraded. We propose that pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program...
  14. ncbi request reprint G protein signaling in yeast: new components, new connections, new compartments
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 314:1412-3. 2006
    ..New tools available in yeast are beginning to uncover new pathway components and interactions and have revealed signaling in unexpected locations within the cell...
  15. ncbi request reprint Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 126:191-203. 2006
    ..These findings reveal two new components of the pheromone signaling pathway. More remarkably, these proteins appear to comprise a preformed effector-G beta subunit assembly and function at the endosome rather than at the plasma membrane...
  16. doi request reprint Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1
    Sarah T Clement
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Sci Signal 6:ra78. 2013
    ..Thus, the same kinases and phosphatase that regulate Snf1 also regulate Gpa1. More broadly, these results indicate that the pheromone signaling and glucose-sensing pathways communicate directly to coordinate cell behavior. ..
  17. pmc Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange factor
    Michael J Lee
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7365, USA
    Curr Biol 18:211-5. 2008
    ..Our data suggest that intracellular proteins function in cooperation with mating pheromones to amplify G protein signaling, thereby leading to full pathway activation...
  18. pmc Structure and function of Vps15 in the endosomal G protein signaling pathway
    Erin J Heenan
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    Biochemistry 48:6390-401. 2009
    ..These findings reveal that the Vps15 Gbeta-like domain serves as a scaffold to assemble Gpa1 and Atg14, whereas the kinase and intermediate domains are required for proper signaling at the endosome...
  19. pmc Selective regulation of MAP kinase signaling by an endomembrane phosphatidylinositol 4-kinase
    Steven D Cappell
    Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:14852-60. 2011
    ..These findings reveal a new regulator of signaling specificity functioning at endomembranes rather than at the plasma membrane...
  20. pmc Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit
    Matthew P Torres
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7260, USA
    J Biol Chem 286:20208-16. 2011
    ..These findings demonstrate that Gpa1 is dynamically regulated. More broadly, they reveal how G proteins can simultaneously regulate, and become regulated by, progression through the cell cycle...
  21. pmc Bistability, stochasticity, and oscillations in the mitogen-activated protein kinase cascade
    Xiao Wang
    Department of Statistics and Operations Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7365, USA
    Biophys J 90:1961-78. 2006
    ..We demonstrate that the inclusion of protein turnover can lead to sustained oscillations of protein concentrations in the absence of feedback regulation. Thus, protein turnover can profoundly influence the output of a signaling pathway...
  22. pmc Proper protein glycosylation promotes mitogen-activated protein kinase signal fidelity
    Evan C Lien
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Biochemistry 52:115-24. 2013
    ..These findings reveal new components of the N-glycosylation machinery needed to ensure MAPK signaling fidelity...
  23. pmc G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase
    Matthew P Torres
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 284:8940-50. 2009
    ....
  24. pmc Functional reconstitution of an atypical G protein heterotrimer and regulator of G protein signaling protein (RGS1) from Arabidopsis thaliana
    Janice C Jones
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:13143-50. 2011
    ..Thus, despite profound differences in the mechanisms of activation, the Arabidopsis G protein is readily inactivated by its cognate RGS protein and forms a stable, GDP-bound, heterotrimeric complex similar to that found in animals...
  25. pmc Yeast dynamically modify their environment to achieve better mating efficiency
    Meng Jin
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Sci Signal 4:ra54. 2011
    ..Thus, budding yeast dynamically remodel their environment to ensure productive responses to an external stimulus and avoid nonproductive cell-cell interactions...
  26. ncbi request reprint Pheromone signaling mechanisms in yeast: a prototypical sex machine
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 306:1508-9. 2004
    ....
  27. ncbi request reprint Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity
    Stephen C Parnell
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599 7260, USA
    Biochemistry 44:8159-66. 2005
    ..This analysis documents phosphorylation of Sst2 by Fus3 and demonstrates that the prevailing model for MAP kinase recognition is valid for a native substrate protein in vivo as well as for small synthetic peptides tested in vitro...
  28. ncbi request reprint Differential regulation of G protein alpha subunit trafficking by mono- and polyubiquitination
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 280:284-91. 2005
    ..These data reveal a strong relationship between the extent of ubiquitination and trafficking of the G protein alpha subunit to its site of degradation...
  29. pmc Mathematical and computational analysis of adaptation via feedback inhibition in signal transduction pathways
    Marcelo Behar
    Department of Physics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Biophys J 93:806-21. 2007
    ..Finally, we present characteristic features for each form of feedback regulation that can aid in their identification...
  30. ncbi request reprint Regulators of G protein signaling and transient activation of signaling: experimental and computational analysis reveals negative and positive feedback controls on G protein activity
    Nan Hao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 0812, USA
    J Biol Chem 278:46506-15. 2003
    ..We identified and quantitated these positive and negative feedback loops, which account for the transient response to external signals observed in vivo...
  31. ncbi request reprint Regulation of Ste7 ubiquitination by Ste11 phosphorylation and the Skp1-Cullin-F-box complex
    Yuqi Wang
    Department of Biochemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 2852, USA
    J Biol Chem 278:22284-9. 2003
    ..Our findings suggest that SCF promotes the ubiquitination and degradation of Ste7, thereby favoring the resumption of cell division cycling after pheromone-induced growth arrest...
  32. pmc Protons as second messenger regulators of G protein signaling
    Daniel G Isom
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell 51:531-8. 2013
    ..Together, our computational, biophysical, and cellular analyses reveal an unexpected function for G proteins as mediators of stress-response signaling. ..
  33. ncbi request reprint Dominant-negative inhibition of pheromone receptor signaling by a single point mutation in the G protein alpha subunit
    Yuh Lin Wu
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599 7260, USA
    J Biol Chem 279:35287-97. 2004
    ..Dominant-negative mutants may be useful in matching specific receptors and their cognate G proteins and in determining mechanisms of G protein signaling specificity...
  34. pmc Site-specific monoubiquitination activates Ras by impeding GTPase-activating protein function
    Rachael Baker
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Struct Mol Biol 20:46-52. 2013
    ..These findings reveal a new mechanism by which Ras can trigger persistent signaling in the absence of receptor activation or an oncogenic mutation...
  35. pmc Differences in intradomain and interdomain motion confer distinct activation properties to structurally similar Gα proteins
    Janice C Jones
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 109:7275-9. 2012
    ..Thus, structurally similar proteins can have distinct atomic motions that confer distinct activation mechanisms...
  36. ncbi request reprint The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160
    Ming Guo
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 12:517-24. 2003
    ..We also show that signaling by activated Gpa1 requires direct coupling to an RNA binding protein Scp160. These findings suggest an additional role for Gpa1 and reveal Scp160 as a component of the mating response pathway in yeast...
  37. pmc Kinetic insulation as an effective mechanism for achieving pathway specificity in intracellular signaling networks
    Marcelo Behar
    Department of Physics, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 104:16146-51. 2007
    ..Our results suggest that multilevel signaling cascades may have evolved to modulate the temporal profile of pathway activity so that stimulus information can be efficiently encoded and transmitted while ensuring signal specificity...
  38. pmc The crystal structure of a self-activating G protein alpha subunit reveals its distinct mechanism of signal initiation
    Janice C Jones
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Sci Signal 4:ra8. 2011
    ..These findings reveal the structural basis for a mechanism for G protein activation in Arabidopsis that is distinct from the well-established mechanism found in animals...
  39. ncbi request reprint Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection
    Scott A Chasse
    Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, 27599 7260, USA
    Methods Enzymol 389:399-409. 2004
    ..This article describes a high-throughput method of analyzing a yeast gene deletion library for novel G-protein signaling modulators using a yeast pheromone pathway-specific reporter...
  40. ncbi request reprint Pheromone-dependent ubiquitination of the mitogen-activated protein kinase kinase Ste7
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 2852, USA
    J Biol Chem 277:15766-72. 2002
    ..These findings reveal a mechanism by which pheromone-triggered ubiquitination of Ste7 can modulate the pheromone response in vivo...
  41. pmc Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Sci Signal 5:re2. 2012
    ..Historically most attention has focused on the Ras-like domain, but emerging evidence reveals that the helical domain is an active participant in G protein signaling...
  42. ncbi request reprint Illuminating Gbeta5 signaling
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 116 Manning Dr, CB 7260, Chapel Hill, NC 27599, USA
    Mol Pharmacol 72:810-1. 2007
    ..The approach used here provides a new strategy to elucidate the spatial and temporal properties of G proteins, including a growing number of atypical Gbetagamma pairings...
  43. doi request reprint RGS proteins the early days
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    Prog Mol Biol Transl Sci 86:1-14. 2009
    ....
  44. ncbi request reprint MAPK kinase kinases (MKKKs) as a target class for small-molecule inhibition to modulate signaling networks and gene expression
    Gary L Johnson
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, NC 27599 7365, USA
    Curr Opin Chem Biol 9:325-31. 2005
    ....
  45. ncbi request reprint Diminishing returns
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Campus Box 7260, Chapel Hill, North Carolina 27599 2852, USA
    Nature 418:591. 2002
  46. pmc Similarities between UDP-glucose and adenine nucleotide release in yeast: involvement of the secretory pathway
    Charles R Esther
    Division of Pediatric Pulmonology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:9269-78. 2008
    ..These studies demonstrate the involvement of the secretory pathway in nucleotide and nucleotide sugar efflux in yeast and offer a powerful model system for further investigation...
  47. ncbi request reprint Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae
    Scott A Burchett
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 277:22156-67. 2002
    ..These findings indicate that Vps36 and Sst2 have opposite and opposing effects on the pheromone and stress response pathways, with Vps36 acting downstream of the G protein and independently of Sst2 RGS activity...
  48. ncbi request reprint Direct identification of a G protein ubiquitination site by mass spectrometry
    Louis A Marotti
    Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Biochemistry 41:5067-74. 2002
    ..These findings indicate that the alpha-helical domain may serve to regulate the turnover of Gpa1...
  49. ncbi request reprint Mathematical modeling of RGS and G-protein regulation in yeast
    Necmettin Yildirim
    Department of Mathematics, University of North Carolina at Chapel Hill, 27599 3250, USA
    Methods Enzymol 389:383-98. 2004
    ..It also illustrates that a complete understanding of signaling pathways requires an investigation of their time-dependent behavior...
  50. pmc Dose-to-duration encoding and signaling beyond saturation in intracellular signaling networks
    Marcelo Behar
    Department of Physics, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Comput Biol 4:e1000197. 2008
    ....
  51. ncbi request reprint Regulation of G protein and mitogen-activated protein kinase signaling by ubiquitination: insights from model organisms
    Yuqi Wang
    Department of Biology, Saint Louis University, 128 Macelwane Hall, 3507 Laclede Ave, St Louis, MO 63103, USA
    Circ Res 99:1305-14. 2006
    ..Similar mechanisms uncovered in other model systems are also briefly discussed to illustrate the importance and universality of signaling regulation by ubiquitination...
  52. ncbi request reprint Identification of allosteric peptide agonists of CXCR4
    Aristidis Sachpatzidis
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:896-907. 2003
    ..111. These results suggest that alternative agonist-binding sites are present on CXCR4 that could be screened to develop molecules for therapeutic use...
  53. ncbi request reprint DEP-domain-mediated regulation of GPCR signaling responses
    Daniel R Ballon
    Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA
    Cell 126:1079-93. 2006
    ..DEP-domain-mediated targeting of effectors and regulators to specific GPCRs provides a means to dictate the nature, duration, and specificity of the response...
  54. ncbi request reprint Purification of RGS protein, Sst2, from Saccharomyces cerevisiae and Escherichia coli
    Tiffany Runyan Garrison
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Methods Enzymol 344:632-47. 2002
  55. ncbi request reprint Analysis of RGS proteins in Saccharomyces cerevisiae
    Ginger A Hoffman
    Interdepartmental Neuroscience Program, Yale University, New Haven, Connecticut 06536, USA
    Methods Enzymol 344:617-31. 2002