HENRIK DOHLMAN

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Site-specific monoubiquitination activates Ras by impeding GTPase-activating protein function
    Rachael Baker
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Struct Mol Biol 20:46-52. 2013
  2. pmc Checkpoints in a yeast differentiation pathway coordinate signaling during hyperosmotic stress
    Michal J Nagiec
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Genet 8:e1002437. 2012
  3. pmc Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Sci Signal 5:re2. 2012
  4. ncbi request reprint Pheromone signaling pathways in yeast
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building Room 430, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci STKE 2006:cm6. 2006
  5. ncbi request reprint MAPK kinase kinases (MKKKs) as a target class for small-molecule inhibition to modulate signaling networks and gene expression
    Gary L Johnson
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, NC 27599 7365, USA
    Curr Opin Chem Biol 9:325-31. 2005
  6. pmc Similarities between UDP-glucose and adenine nucleotide release in yeast: involvement of the secretory pathway
    Charles R Esther
    Division of Pediatric Pulmonology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:9269-78. 2008
  7. ncbi request reprint G proteins and pheromone signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Annu Rev Physiol 64:129-52. 2002
  8. doi request reprint A scaffold makes the switch
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci Signal 1:pe46. 2008
  9. ncbi request reprint Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Biol Chem 281:1964-9. 2006
  10. pmc Bistability, stochasticity, and oscillations in the mitogen-activated protein kinase cascade
    Xiao Wang
    Department of Statistics and Operations Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7365, USA
    Biophys J 90:1961-78. 2006

Research Grants

  1. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2002
  2. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2004
  3. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2007
  4. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2007
  5. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2009
  6. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2009
  7. Modeling Feedback Regulation of Cell Signaling
    HENRIK DOHLMAN; Fiscal Year: 2009
  8. Modeling Feedback Regulation of Cell Signaling
    Henrik G Dohlman; Fiscal Year: 2010
  9. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2007
  10. Gordon Conference on "Phosphorylation and G Protein Signaling Networks"
    HENRIK DOHLMAN; Fiscal Year: 2007

Collaborators

  • Lewis C Cantley
  • Gary Johnson
  • EDUARDO RODOLFO LAZAROWSKI
  • C R Esther
  • Lee Graves
  • Yuqi Wang
  • STANLEY H FIELDS
  • Jason W Locasale
  • VYTAS BANKAITIS
  • Elias Lolis
  • Nan Hao
  • Timothy C Elston
  • Marcelo Behar
  • Stephen C Parnell
  • Janna E Slessareva
  • Michal J Nagiec
  • Scott A Chasse
  • Rachael Baker
  • Necmettin Yildirim
  • Louis A Marotti
  • Scott A Burchett
  • Tiffany Runyan Garrison
  • Michael J Lee
  • Corinne E Zeller
  • Christoph H Borchers
  • Matthew P Torres
  • Xiao Wang
  • Daniel R Ballon
  • Beverly Errede
  • Paul Flanary
  • Seth Maleri
  • Yuh Lin Wu
  • Ming Guo
  • Aristidis Sachpatzidis
  • Peter Uetz
  • Christopher Aston
  • Ginger A Hoffman
  • Brian Kuhlman
  • Steven M Lewis
  • Sharon L Campbell
  • Atsuo T Sasaki
  • Emily M Wilkerson
  • Jeffrey Hasty
  • Sujata Nayak
  • Ryan H Shanks
  • Sheri M Routt
  • James B Konopka
  • Paul L Flanary
  • Jeremy Thorner
  • Jiyoung Y Cha
  • Brenda Temple
  • David P Siderovski
  • Douglas P Gladue
  • Lee Kiang
  • Qingyuan Ge
  • Elizabeth A Hackett
  • T Kendall Harden
  • Shelley B Hooks
  • S Johannes R Rajarao
  • Kathleen Young
  • Christine Dyke
  • Benjamin K Benton
  • Hua Wang
  • John P Manfredi
  • Andrew Hamilton
  • Rick Newitt
  • Ruedi Aebersold
  • Kathleen H Young
  • Donald M Apanovitch
  • Lixin Jiang

Detail Information

Publications37

  1. pmc Site-specific monoubiquitination activates Ras by impeding GTPase-activating protein function
    Rachael Baker
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Struct Mol Biol 20:46-52. 2013
    ..These findings reveal a new mechanism by which Ras can trigger persistent signaling in the absence of receptor activation or an oncogenic mutation...
  2. pmc Checkpoints in a yeast differentiation pathway coordinate signaling during hyperosmotic stress
    Michal J Nagiec
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Genet 8:e1002437. 2012
    ..These findings reveal how an environmental stress signal is able to postpone responsiveness to a competing differentiation signal, by acting on multiple pathway components, in a coordinated manner...
  3. pmc Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Sci Signal 5:re2. 2012
    ..Historically most attention has focused on the Ras-like domain, but emerging evidence reveals that the helical domain is an active participant in G protein signaling...
  4. ncbi request reprint Pheromone signaling pathways in yeast
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building Room 430, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci STKE 2006:cm6. 2006
    ..This updated Connections Map in the Database of Cell Signaling includes several major revisions to this prototypical signal response pathway...
  5. ncbi request reprint MAPK kinase kinases (MKKKs) as a target class for small-molecule inhibition to modulate signaling networks and gene expression
    Gary L Johnson
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, NC 27599 7365, USA
    Curr Opin Chem Biol 9:325-31. 2005
    ....
  6. pmc Similarities between UDP-glucose and adenine nucleotide release in yeast: involvement of the secretory pathway
    Charles R Esther
    Division of Pediatric Pulmonology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:9269-78. 2008
    ..These studies demonstrate the involvement of the secretory pathway in nucleotide and nucleotide sugar efflux in yeast and offer a powerful model system for further investigation...
  7. ncbi request reprint G proteins and pheromone signaling
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Annu Rev Physiol 64:129-52. 2002
    ....
  8. doi request reprint A scaffold makes the switch
    Henrik G Dohlman
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Sci Signal 1:pe46. 2008
    ..New findings reveal that the graded-to-binary conversion can be turned on or off, depending on the location of the scaffold within the cell...
  9. ncbi request reprint Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Biol Chem 281:1964-9. 2006
    ..In the absence of sumoylation Tec1 is more rapidly degraded. We propose that pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program...
  10. pmc Bistability, stochasticity, and oscillations in the mitogen-activated protein kinase cascade
    Xiao Wang
    Department of Statistics and Operations Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7365, USA
    Biophys J 90:1961-78. 2006
    ..We demonstrate that the inclusion of protein turnover can lead to sustained oscillations of protein concentrations in the absence of feedback regulation. Thus, protein turnover can profoundly influence the output of a signaling pathway...
  11. pmc Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae
    Scott A Chasse
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Eukaryot Cell 5:330-46. 2006
    ..These findings suggest that Sst2 is the principal regulator of Gpa1-mediated signaling in vivo but that other proteins also contribute in distinct ways to pathway regulation...
  12. pmc Dose-to-duration encoding and signaling beyond saturation in intracellular signaling networks
    Marcelo Behar
    Department of Physics, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS Comput Biol 4:e1000197. 2008
    ....
  13. ncbi request reprint Regulation of G protein and mitogen-activated protein kinase signaling by ubiquitination: insights from model organisms
    Yuqi Wang
    Department of Biology, Saint Louis University, 128 Macelwane Hall, 3507 Laclede Ave, St Louis, MO 63103, USA
    Circ Res 99:1305-14. 2006
    ..Similar mechanisms uncovered in other model systems are also briefly discussed to illustrate the importance and universality of signaling regulation by ubiquitination...
  14. ncbi request reprint A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway
    Nan Hao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Curr Biol 17:659-67. 2007
    ..For instance in yeast, the mitogen-activated protein (MAP) kinase Hog1 is activated and inactivated within minutes, even when the osmotic-stress stimulus is sustained...
  15. pmc Regulation of cell signaling dynamics by the protein kinase-scaffold Ste5
    Nan Hao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 30:649-56. 2008
    ..We propose that scaffold proteins serve to modulate the temporal and dose-response behavior of the MAP kinase...
  16. pmc Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange factor
    Michael J Lee
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7365, USA
    Curr Biol 18:211-5. 2008
    ..Our data suggest that intracellular proteins function in cooperation with mating pheromones to amplify G protein signaling, thereby leading to full pathway activation...
  17. ncbi request reprint Direct identification of a G protein ubiquitination site by mass spectrometry
    Louis A Marotti
    Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Biochemistry 41:5067-74. 2002
    ..These findings indicate that the alpha-helical domain may serve to regulate the turnover of Gpa1...
  18. ncbi request reprint Pheromone signaling mechanisms in yeast: a prototypical sex machine
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 306:1508-9. 2004
    ....
  19. pmc Persistent activation by constitutive Ste7 promotes Kss1-mediated invasive growth but fails to support Fus3-dependent mating in yeast
    Seth Maleri
    Department of Biochemistry and Biophysics, CB 7260, 512 ME Jones, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Mol Cell Biol 24:9221-38. 2004
    ..This scaffold-independent activation of Kss1 by constitutive Ste7 and the existence of mechanisms for pathway-specific promoter discrimination impose a unique developmental fate independently of any distinguishing external stimuli...
  20. ncbi request reprint Pheromone-dependent ubiquitination of the mitogen-activated protein kinase kinase Ste7
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 2852, USA
    J Biol Chem 277:15766-72. 2002
    ..These findings reveal a mechanism by which pheromone-triggered ubiquitination of Ste7 can modulate the pheromone response in vivo...
  21. ncbi request reprint Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae
    Scott A Burchett
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 277:22156-67. 2002
    ..These findings indicate that Vps36 and Sst2 have opposite and opposing effects on the pheromone and stress response pathways, with Vps36 acting downstream of the G protein and independently of Sst2 RGS activity...
  22. ncbi request reprint Regulation of Ste7 ubiquitination by Ste11 phosphorylation and the Skp1-Cullin-F-box complex
    Yuqi Wang
    Department of Biochemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 2852, USA
    J Biol Chem 278:22284-9. 2003
    ..Our findings suggest that SCF promotes the ubiquitination and degradation of Ste7, thereby favoring the resumption of cell division cycling after pheromone-induced growth arrest...
  23. ncbi request reprint Mathematical modeling of RGS and G-protein regulation in yeast
    Necmettin Yildirim
    Department of Mathematics, University of North Carolina at Chapel Hill, 27599 3250, USA
    Methods Enzymol 389:383-98. 2004
    ..It also illustrates that a complete understanding of signaling pathways requires an investigation of their time-dependent behavior...
  24. ncbi request reprint Regulators of G protein signaling and transient activation of signaling: experimental and computational analysis reveals negative and positive feedback controls on G protein activity
    Nan Hao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 0812, USA
    J Biol Chem 278:46506-15. 2003
    ..We identified and quantitated these positive and negative feedback loops, which account for the transient response to external signals observed in vivo...
  25. ncbi request reprint Identification of allosteric peptide agonists of CXCR4
    Aristidis Sachpatzidis
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:896-907. 2003
    ..111. These results suggest that alternative agonist-binding sites are present on CXCR4 that could be screened to develop molecules for therapeutic use...
  26. ncbi request reprint Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity
    Stephen C Parnell
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599 7260, USA
    Biochemistry 44:8159-66. 2005
    ..This analysis documents phosphorylation of Sst2 by Fus3 and demonstrates that the prevailing model for MAP kinase recognition is valid for a native substrate protein in vivo as well as for small synthetic peptides tested in vitro...
  27. ncbi request reprint Purification of RGS protein, Sst2, from Saccharomyces cerevisiae and Escherichia coli
    Tiffany Runyan Garrison
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Methods Enzymol 344:632-47. 2002
  28. pmc Kinetic insulation as an effective mechanism for achieving pathway specificity in intracellular signaling networks
    Marcelo Behar
    Department of Physics, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 104:16146-51. 2007
    ..Our results suggest that multilevel signaling cascades may have evolved to modulate the temporal profile of pathway activity so that stimulus information can be efficiently encoded and transmitted while ensuring signal specificity...
  29. ncbi request reprint The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast
    Corinne E Zeller
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:25168-76. 2007
    ..Our findings reveal the existence of an unusual Gbeta subunit, one having multiple functions within the cell in addition to serving as a signal transducer for cell surface receptors and intracellular effectors...
  30. ncbi request reprint The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160
    Ming Guo
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 12:517-24. 2003
    ..We also show that signaling by activated Gpa1 requires direct coupling to an RNA binding protein Scp160. These findings suggest an additional role for Gpa1 and reveal Scp160 as a component of the mating response pathway in yeast...
  31. ncbi request reprint Dominant-negative inhibition of pheromone receptor signaling by a single point mutation in the G protein alpha subunit
    Yuh Lin Wu
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599 7260, USA
    J Biol Chem 279:35287-97. 2004
    ..Dominant-negative mutants may be useful in matching specific receptors and their cognate G proteins and in determining mechanisms of G protein signaling specificity...
  32. ncbi request reprint G protein signaling in yeast: new components, new connections, new compartments
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 314:1412-3. 2006
    ..New tools available in yeast are beginning to uncover new pathway components and interactions and have revealed signaling in unexpected locations within the cell...
  33. ncbi request reprint DEP-domain-mediated regulation of GPCR signaling responses
    Daniel R Ballon
    Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA
    Cell 126:1079-93. 2006
    ..DEP-domain-mediated targeting of effectors and regulators to specific GPCRs provides a means to dictate the nature, duration, and specificity of the response...
  34. ncbi request reprint Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 126:191-203. 2006
    ..These findings reveal two new components of the pheromone signaling pathway. More remarkably, these proteins appear to comprise a preformed effector-G beta subunit assembly and function at the endosome rather than at the plasma membrane...
  35. ncbi request reprint Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection
    Scott A Chasse
    Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, 27599 7260, USA
    Methods Enzymol 389:399-409. 2004
    ..This article describes a high-throughput method of analyzing a yeast gene deletion library for novel G-protein signaling modulators using a yeast pheromone pathway-specific reporter...
  36. ncbi request reprint Differential regulation of G protein alpha subunit trafficking by mono- and polyubiquitination
    Yuqi Wang
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 280:284-91. 2005
    ..These data reveal a strong relationship between the extent of ubiquitination and trafficking of the G protein alpha subunit to its site of degradation...
  37. ncbi request reprint Analysis of RGS proteins in Saccharomyces cerevisiae
    Ginger A Hoffman
    Interdepartmental Neuroscience Program, Yale University, New Haven, Connecticut 06536, USA
    Methods Enzymol 344:617-31. 2002

Research Grants29

  1. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2002
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  2. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2004
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  3. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2007
    ..Delineating such new pathways is the principal objective of this proposal. ..
  4. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2007
    ..abstract_text> ..
  5. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2009
    ....
  6. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2009
    ..Delineating such new pathways is the principal objective of this proposal. ..
  7. Modeling Feedback Regulation of Cell Signaling
    HENRIK DOHLMAN; Fiscal Year: 2009
    ..The broader objective is to understand the role of spatial and temporal information in cell signaling, and eventually predict what drug treatments will be most effective in combating human diseases. ..
  8. Modeling Feedback Regulation of Cell Signaling
    Henrik G Dohlman; Fiscal Year: 2010
    ..The broader objective is to understand the role of spatial and temporal information in cell signaling, and eventually predict what drug treatments will be most effective in combating human diseases. ..
  9. G Protein signaling at the endosome
    HENRIK DOHLMAN; Fiscal Year: 2007
    ....
  10. Gordon Conference on "Phosphorylation and G Protein Signaling Networks"
    HENRIK DOHLMAN; Fiscal Year: 2007
    ..To emphasize this goal, 8 poster presentations that complement the themes of the meeting will be selected for short oral presentations during the main sessions. ..
  11. Modeling Feedback Regulation of Cell Signaling
    HENRIK DOHLMAN; Fiscal Year: 2007
    ..An integrated computational and experimental analysis of the pheromone regulation in yeast will eventually lead to improved predictive models of signaling events in more complex organisms including humans. ..
  12. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2001
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  13. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2003
    ..These experiments will reveal how cell signaling is regulated through feedback regulation of an effector kinase, initially in yeast and eventually in mammalian cells. ..
  14. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2003
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  15. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2004
    ..These experiments will reveal how cell signaling is regulated through feedback regulation of an effector kinase, initially in yeast and eventually in mammalian cells. ..
  16. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2004
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  17. Modeling Feedback Regulation of Cell Signaling
    HENRIK DOHLMAN; Fiscal Year: 2006
    ..An integrated computational and experimental analysis of the pheromone regulation in yeast will eventually lead to improved predictive models of signaling events in more complex organisms including humans. ..
  18. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2006
    ..These experiments will reveal how cell signaling is regulated through feedback regulation of an effector kinase, initially in yeast and eventually in mammalian cells. ..
  19. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    HENRIK DOHLMAN; Fiscal Year: 2005
    ..These experiments will reveal how cell signaling is regulated through feedback regulation of an effector kinase, initially in yeast and eventually in mammalian cells. ..
  20. G Protein signaling at the endosome
    Henrik G Dohlman; Fiscal Year: 2010
    ....
  21. REGULATION OF SIGNALING BY G PROTEIN-BINDING PROTEINS
    HENRIK DOHLMAN; Fiscal Year: 2000
    ..The molecular and cellular consequences of each modification will be evaluated using standard assays of RGS and G protein function. ..
  22. REGULATORS OF G PROTEIN SIGNALING IN YEAST
    Henrik G Dohlman; Fiscal Year: 2010
    ..Delineating such new pathways is the principal objective of this proposal. ..
  23. Modeling Feedback Regulation of Cell Signaling
    Henrik G Dohlman; Fiscal Year: 2010
    ..The broader objective is to understand the role of spatial and temporal information in cell signaling, and eventually predict what drug treatments will be most effective in combating human diseases. ..