Roberto Docampo

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. pmc Acidocalcisomes
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens 30602, USA
    Cell Calcium 50:113-9. 2011
  2. ncbi request reprint Acidocalcisomes - conserved from bacteria to man
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, Georgia 30602, USA
    Nat Rev Microbiol 3:251-61. 2005
  3. pmc Evidence for the role of vacuolar soluble pyrophosphatase and inorganic polyphosphate in Trypanosoma cruzi persistence
    Melina Galizzi
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA, 30602, USA
    Mol Microbiol 90:699-715. 2013
  4. pmc The role of acidocalcisomes in the stress response of Trypanosoma cruzi
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA, USA
    Adv Parasitol 75:307-24. 2011
  5. pmc Calcium- and polyphosphate-containing acidic granules of sea urchin eggs are similar to acidocalcisomes, but are not the targets for NAADP
    Isabela B Ramos
    Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, 30602, USA
    Biochem J 429:485-95. 2010
  6. pmc Hyperosmotic stress induces aquaporin-dependent cell shrinkage, polyphosphate synthesis, amino acid accumulation, and global gene expression changes in Trypanosoma cruzi
    Zhu Hong Li
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 286:43959-71. 2011
  7. doi request reprint Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk
    Isabela B Ramos
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Biol Cell 102:421-34. 2010
  8. pmc Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA 30602, USA
    Philos Trans R Soc Lond B Biol Sci 365:775-84. 2010
  9. pmc The role of acidocalcisomes in parasitic protists
    Silvia N J Moreno
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, 30602, USA
    J Eukaryot Microbiol 56:208-13. 2009
  10. pmc The acidocalcisome as a target for chemotherapeutic agents in protozoan parasites
    Roberto Docampo
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Curr Pharm Des 14:882-8. 2008

Research Grants

  1. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2010
  2. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2010
  3. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2003
  4. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2006
  5. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2007
  6. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2009
  7. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2009
  8. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2009
  9. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2002
  10. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2001

Collaborators

  • Veronica Jimenez
  • Silvia N Moreno
  • Wanderley de Souza
  • Javier G De Gaudenzi
  • Rick L Tarleton
  • Douglas A Pace
  • Philip J Rosenthal
  • Timothy E Long
  • Jessica C Kissinger
  • ANN LEFURGEY
  • Simon L Croft
  • Eric Oldfield
  • Marlene Benchimol
  • J A Urbina
  • Peter Rohloff
  • Kildare Miranda
  • Shuhong Luo
  • Guozhong Huang
  • Mauricio Vieira
  • Jianmin Fang
  • Zhu Hong Li
  • Felix A Ruiz
  • Paul N Ulrich
  • Sergio H Szajnman
  • Melina Galizzi
  • Maria Laura Salto
  • Vicente de Paulo Martins
  • Isabela B Ramos
  • Norma Marchesini
  • Claudia O Rodrigues
  • Noelia Lander
  • Juan B Rodriguez
  • Andrea Montalvetti
  • Manfredo Seufferheld
  • Marcela Ferella
  • Nicole Van Der Heyden
  • Sayantanee Niyogi
  • Esteban L Ravaschino
  • Lia C Soares Medeiros
  • Vanina E Alvarez
  • Celso Sant'Anna
  • Sharon King-Keller
  • Ednildo A Machado
  • Anibal E Vercesi
  • Esteban J Bontempi
  • Stephanie A Smith
  • Rosa M de Lederkremer
  • Evangelia Kotsikorou
  • Norbert Bakalara
  • Orlando Grillo
  • Anibal Vercesi
  • Christopher R Lea
  • Guillaume Lemercier
  • Helmut Plattner
  • Youhong Wang
  • Noris Rodriguez
  • Juan Mucci
  • David A Scott
  • Vanessa Yardley
  • Samarchith P Kurup
  • Oscar Campetella
  • Rosa Martinez
  • Brian N Bailey
  • Laura Reiss
  • Jessica Brewer
  • Michael B Martin
  • Benjamin Moreno
  • Andrew P Thomas
  • Paula J Bartlett
  • Juan M Bustamante
  • Haiming Wang
  • Alberto C Frasch
  • Nicolás Mendiondo
  • Juan J Cazzulo
  • Kristen Moles
  • Vicente P Martins
  • Miyoung Park
  • Dalis Collins
  • James Atwood
  • Ron Orlando
  • Dianne L Wellems
  • Alberto C C Frasch
  • Yonghui Zhang
  • Xiaochuan Yang
  • Fu Yang Lin
  • Michael Okura
  • Minyong Li
  • David M Engman
  • Alyssa Smith
  • Binghe Wang

Detail Information

Publications66

  1. pmc Acidocalcisomes
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens 30602, USA
    Cell Calcium 50:113-9. 2011
    ..Acidocalcisomes interact with other organelles in a number of organisms suggesting their association with the endosomal/lysosomal pathway, and are considered part of the lysosome-related group of organelles...
  2. ncbi request reprint Acidocalcisomes - conserved from bacteria to man
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, Georgia 30602, USA
    Nat Rev Microbiol 3:251-61. 2005
    ..Here, we review acidocalcisome ultrastructure, composition and function in different trypanosomatids and other organisms...
  3. pmc Evidence for the role of vacuolar soluble pyrophosphatase and inorganic polyphosphate in Trypanosoma cruzi persistence
    Melina Galizzi
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA, 30602, USA
    Mol Microbiol 90:699-715. 2013
    ....
  4. pmc The role of acidocalcisomes in the stress response of Trypanosoma cruzi
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA, USA
    Adv Parasitol 75:307-24. 2011
    ..This pathway is an excellent target for chemotherapy as shown by the effects of phosphodiesterase C inhibitors on parasite survival...
  5. pmc Calcium- and polyphosphate-containing acidic granules of sea urchin eggs are similar to acidocalcisomes, but are not the targets for NAADP
    Isabela B Ramos
    Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, 30602, USA
    Biochem J 429:485-95. 2010
    ....
  6. pmc Hyperosmotic stress induces aquaporin-dependent cell shrinkage, polyphosphate synthesis, amino acid accumulation, and global gene expression changes in Trypanosoma cruzi
    Zhu Hong Li
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 286:43959-71. 2011
    ....
  7. doi request reprint Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk
    Isabela B Ramos
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Biol Cell 102:421-34. 2010
    ....
  8. pmc Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA 30602, USA
    Philos Trans R Soc Lond B Biol Sci 365:775-84. 2010
    ..Storage of phosphate and cations may represent the ancestral physiological function of acidocalcisomes, with cation and pH homeostasis and osmoregulatory functions derived following the divergence of prokaryotes and eukaryotes...
  9. pmc The role of acidocalcisomes in parasitic protists
    Silvia N J Moreno
    Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, 30602, USA
    J Eukaryot Microbiol 56:208-13. 2009
    ..g. the contractile vacuole in Trypanosoma cruzi, and other vacuoles observed in Toxoplasma gondii, suggests that these cellular compartments may be associated with the endosomal/lysosomal pathway...
  10. pmc The acidocalcisome as a target for chemotherapeutic agents in protozoan parasites
    Roberto Docampo
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Curr Pharm Des 14:882-8. 2008
    ..The mechanism of action of bisphosphonates, however, is by inhibition of the isoprenoid pathway and more specifically the prenyl diphosphate synthases...
  11. pmc Adaptor protein-3 (AP-3) complex mediates the biogenesis of acidocalcisomes and is essential for growth and virulence of Trypanosoma brucei
    Guozhong Huang
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 286:36619-30. 2011
    ..Similar results were obtained by knocking down the expression of the δ subunit of AP-3. These results indicate that AP-3 is essential for the biogenesis of acidocalcisomes and for growth and virulence of T. brucei...
  12. pmc Acidocalcisomes of Trypanosoma brucei have an inositol 1,4,5-trisphosphate receptor that is required for growth and infectivity
    Guozhong Huang
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 110:1887-92. 2013
    ....
  13. ncbi request reprint Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation
    Jianmin Fang
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Paul D Coverdell Biomedical and Health Sciences Center, Athens, Georgia 30602, USA
    J Biol Chem 282:32501-10. 2007
    ..This was accompanied by a delayed regulatory volume decrease after hyposmotic stress. These results support the role of poly P in T. cruzi osmoregulation...
  14. pmc Identification of contractile vacuole proteins in Trypanosoma cruzi
    Paul N Ulrich
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 6:e18013. 2011
    ..The CV localization of the proteins investigated revealed potential novel roles of these organelles in phosphate metabolism and provided information on the potential participation of adaptor protein complexes in their biogenesis...
  15. doi request reprint The acidocalcisome vacuolar transporter chaperone 4 catalyzes the synthesis of polyphosphate in insect-stages of Trypanosoma brucei and T. cruzi
    Paul N Ulrich
    Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA Department of Biology, Georgia State University, Atlanta, GA, 30303, USA
    J Eukaryot Microbiol 61:155-65. 2014
    ..These results suggest that this trypanosome enzyme is an important component of a polyphosphate synthase complex that utilizes ATP to synthesize and translocate polyphosphate to acidocalcisomes in insect stages of these parasites. ..
  16. pmc Trypanosoma brucei vacuolar transporter chaperone 4 (TbVtc4) is an acidocalcisome polyphosphate kinase required for in vivo infection
    Noelia Lander
    From the Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602
    J Biol Chem 288:34205-16. 2013
    ..The mutant also exhibited a defect in volume recovery under osmotic stress conditions in vitro, underscoring the relevance of polyP in osmoregulation. ..
  17. pmc A 43-nucleotide U-rich element in 3'-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotes
    Zhu Hong Li
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 287:19058-69. 2012
    ..These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage...
  18. pmc Acylation-dependent export of Trypanosoma cruzi phosphoinositide-specific phospholipase C to the outer surface of amastigotes
    Vicente de Paulo Martins
    Department of Cellular Biology, University of Georgia, Center for Tropical and Emerging Global Diseases, Athens, Georgia 30602, USA
    J Biol Chem 285:30906-17. 2010
    ..Taken together, these results indicate that N-terminal acyl modifications serve as a molecular addressing system for sending TcPI-PLC to the outer surface of the cell...
  19. pmc Ablation of a small transmembrane protein of Trypanosoma brucei (TbVTC1) involved in the synthesis of polyphosphate alters acidocalcisome biogenesis and function, and leads to a cytokinesis defect
    Jianmin Fang
    Center for Tropical and Emerging Global Disease and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Biochem J 407:161-70. 2007
    ..A decrease in the poly P content would lead to osmotic sensitivity and defects in cytokinesis...
  20. pmc Molecular and electrophysiological characterization of a novel cation channel of Trypanosoma cruzi
    Veronica Jimenez
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 8:e1002750. 2012
    ..cruzi after recombinant expression in bacteria. The peculiar characteristics of TcCat could be important for the development of specific inhibitors with therapeutic potential against trypanosomes...
  21. pmc Phosphonium lipocations as antiparasitic agents
    Timothy E Long
    Department of Pharmaceutical and Biomedical Sciences and Center for Drug Discovery, University of Georgia, Athens, GA 30602, USA
    Bioorg Med Chem Lett 22:2976-9. 2012
    ..Optimal phthalimides and 1,4-naphthoquinone-based lipocations were active in vitro at mid-high nM concentrations against P. falciparum and low μM concentrations against T. cruzi...
  22. pmc New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists
    Roberto Docampo
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, USA
    Int Rev Cell Mol Biol 305:69-113. 2013
    ..We also review the current state of knowledge on the composition of these organelles and their other roles in calcium homeostasis and protein trafficking. ..
  23. pmc Trypanosomes and the solution to a 50-year mitochondrial calcium mystery
    Roberto Docampo
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30620, USA
    Trends Parasitol 28:31-7. 2012
    ..These results add another success story to the already substantial contributions of trypanosomes to mammalian biochemistry...
  24. pmc Developmental expression of a Trypanosoma cruzi phosphoinositide-specific phospholipase C in amastigotes and stimulation of host phosphoinositide hydrolysis
    Vicente de Paulo Martins
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602 2607, USA
    Infect Immun 78:4206-12. 2010
    ..The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction...
  25. pmc Chemical validation of phosphodiesterase C as a chemotherapeutic target in Trypanosoma cruzi, the etiological agent of Chagas' disease
    Sharon King-Keller
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, 350B Paul D Coverdell Center, 500 D W Brooks Dr, Athens, GA 30602, USA
    Antimicrob Agents Chemother 54:3738-45. 2010
    ..The compounds found could be useful tools in the study of osmoregulation in T. cruzi. In addition, their further optimization could result in the development of new drugs against Chagas' disease and other trypanosomiases...
  26. ncbi request reprint Rab11 Regulates Trafficking of Trans-sialidase to the Plasma Membrane through the Contractile Vacuole Complex of Trypanosoma cruzi
    Sayantanee Niyogi
    Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS Pathog 10:e1004224. 2014
    ....
  27. pmc Essential regulation of cell bioenergetics in Trypanosoma brucei by the mitochondrial calcium uniporter
    Guozhong Huang
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    Nat Commun 4:2865. 2013
    ..The requirement of TbMCU for proline and pyruvate metabolism in procyclic and bloodstream forms, respectively, reveals its role in regulation of mitochondrial bioenergetics. ..
  28. doi request reprint Special section on novel organelles in parasitic protozoa
    Roberto Docampo
    Center for Tropical and Emerging Global Diseases, Paul D Coverdell Center for Biomedical and Health Sciences, 500 DW Brooks Drive, Athens, GA 30606, USA
    Exp Parasitol 118:1. 2008
  29. ncbi request reprint Molecular characterization of Trypanosoma brucei P-type H+-ATPases
    Shuhong Luo
    Center for Tropical and Emerging Global Diseases and the Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 281:21963-73. 2006
    ..No evidence was found of an intracellular P-type H+-ATPase activity. These results establish that T. brucei H+-ATPases are plasma membrane enzymes essential for parasite viability...
  30. ncbi request reprint Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideum
    Norma Marchesini
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 277:8146-53. 2002
    ..Together, these results suggest that the mass-dense granules of D. discoideum are homologous to the acidocalcisomes described in protozoan parasites and are linked to the function of the contractile vacuole...
  31. ncbi request reprint Calcium regulation in protozoan parasites
    Silvia N J Moreno
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, IL 61802, USA
    Curr Opin Microbiol 6:359-64. 2003
    ..Recent findings have shed light on the function of these transporters, the roles that they play in cellular metabolism and their potential use for targeting them for new therapies...
  32. ncbi request reprint The H(+)-pyrophosphatase of Rhodospirillum rubrum is predominantly located in polyphosphate-rich acidocalcisomes
    Manfredo Seufferheld
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:51193-202. 2004
    ..Taken together, these results suggest that the H(+)-PPase of R. rubrum has two distinct roles depending on its location acting as an intracellular proton pump in acidocalcisomes but in PPi synthesis in the chromatophore membranes...
  33. ncbi request reprint Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotes
    Manfredo Seufferheld
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 278:29971-8. 2003
    ..The results suggest that acidocalcisomes arose before the prokaryotic and eukaryotic lineages diverged...
  34. ncbi request reprint Polyphosphate content and fine structure of acidocalcisomes of Plasmodium falciparum
    Felix A Ruiz
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, IL 61802, USA
    Microsc Microanal 10:563-7. 2004
    ..Taken together, these results provide evidence that acidocalcisomes from Plasmodium falciparum do not differ significantly from acidocalcisomes of other apicomplexan parasites...
  35. ncbi request reprint An acidocalcisomal exopolyphosphatase from Leishmania major with high affinity for short chain polyphosphate
    Claudia O Rodrigues
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 277:50899-906. 2002
    ..4 +/- 3.0 mm) and long chain polyP (55.9 +/- 5.6 mm) were detected in L. major promastigotes. The unique characteristics of LmPPX and L. major polyP metabolism may facilitate the development of novel antileishmanial agents...
  36. pmc Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
    Maria Laura Salto
    Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, IL 61802, United States
    Mol Biochem Parasitol 158:120-30. 2008
    ..Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition...
  37. ncbi request reprint Acidocalcisomes of Phytomonas françai possess distinct morphological characteristics and contain iron
    Kildare Miranda
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av Brigadeiro Trompovski, S N, Bloco G, Cidade Universitaria, 21949 900, Rio de Janeiro, RJ, Brazil
    Microsc Microanal 10:647-55. 2004
    ..Together, these results suggest that the electron-dense organelles of P. françai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content...
  38. ncbi request reprint Acidocalcisomes of trypanosomatids have species-specific elemental composition
    Kildare Miranda
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Univesidade Federal do Rio de Janeiro, Av Brigadeiro Trompovski, S N, Bloco G, Cidade Universitaria, 21949 900, Rio de Janeiro, RJ, Brazil
    Protist 155:395-405. 2004
    ..Taken together, the results strongly indicate a species-specific composition of acidocalcisomes in trypanosomatid parasites...
  39. ncbi request reprint A pyrophosphatase regulating polyphosphate metabolism in acidocalcisomes is essential for Trypanosoma brucei virulence in mice
    Guillaume Lemercier
    Laboratoire de Genomique Fonctionnelle des Trypanosomatides, UMR CNRS 5162, 146, rue Leo Saignat, 33076 Bordeaux, France
    J Biol Chem 279:3420-5. 2004
    ..BFiVSP1 did not cause acute virulent infection in mice, demonstrating that TbVSP1 is essential for growth of bloodstream forms in the mammalian host...
  40. ncbi request reprint Synthesis and biological evaluation of 1-amino-1,1-bisphosphonates derived from fatty acids against Trypanosoma cruzi targeting farnesyl pyrophosphate synthase
    Sergio H Szajnman
    Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
    Bioorg Med Chem Lett 15:4685-90. 2005
    ..cruzi farnesyl pyrophosphate synthase. As bisphosphonate-containing drugs are FDA-approved for the treatment of bone resorption disorders, their potential innocuousness makes them good candidates to control tropical diseases...
  41. ncbi request reprint Bisphosphonates derived from fatty acids are potent inhibitors of Trypanosoma cruzi farnesyl pyrophosphate synthase
    Sergio H Szajnman
    Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
    Bioorg Med Chem Lett 13:3231-5. 2003
    ..As bisphosphonates are FDA clinically approved for treatment of bone resorption disorders, their potential innocuousness makes them good candidates to control tropical diseases...
  42. ncbi request reprint A plasma membrane P-type H(+)-ATPase regulates intracellular pH in Leishmania mexicana amazonensis
    Norma Marchesini
    Laboratory of Molecular Parasitology, Department of Pathobiology, College of Verterinary Medicine, University of Illinois at Urbana Champaign, 2001 South, Lincoln Avenue, Urbana, IL 61802, USA
    Mol Biochem Parasitol 119:225-36. 2002
    ..80+/-0.03 to 7.25+/-0.09 and induce membrane hyperpolarization. No evidence was found of the possible involvement of a K(+)/H(+)-ATPase in intracellular pH regulation in both developmental stages of L. m. amazonensis...
  43. ncbi request reprint Proton and sodium pumps regulate the plasma membrane potential of different stages of Trypanosoma cruzi
    Nicole Van Der Heyden
    Laboratory of Molecular Parasitology, Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
    Mol Biochem Parasitol 120:127-39. 2002
    ..The presence of K(+) channels in amastigotes and a sodium pump in trypomastigotes, in addition to the H(+)-ATPase, could provide important new targets for trypanocidal drug development...
  44. pmc Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote
    Maria Laura Salto
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, College of Veterinary Medicine, University of Illinois at Urbana Champaign, 61802, USA
    Eukaryot Cell 2:756-68. 2003
    ..Our data suggest that inositolphosphoceramide is implicated in T. cruzi differentiation and that its metabolism could provide important targets for the development of antiparasitic therapies...
  45. pmc In vivo activities of farnesyl pyrophosphate synthase inhibitors against Leishmania donovani and Toxoplasma gondii
    Vanessa Yardley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    Antimicrob Agents Chemother 46:929-31. 2002
    ..donovani by intravenous administration. Risedronate had a 50% effective dosage of five 2.6-mg/kg of body weight intraperitoneal doses against L. donovani-infected mice but was less effective against T. gondii-infected mice...
  46. ncbi request reprint Significant differences between procyclic and bloodstream forms of Trypanosoma brucei in the maintenance of their plasma membrane potential
    Nicole Van Der Heyden
    Department of Pathobiology, University of Illinois at Urbana Champaign, 61802, USA
    J Eukaryot Microbiol 49:407-13. 2002
    ..Thus, there are significant differences between procyclic and bloodstream forms in the maintenance of the deltapsi and in their permeability to cations...
  47. ncbi request reprint A proton pumping pyrophosphatase in the Golgi apparatus and plasma membrane vesicles of Trypanosoma cruzi
    Rosa Martinez
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
    Mol Biochem Parasitol 120:205-13. 2002
    ..Taken together, these results are consistent with the presence of a functional H(+)-PPase in the plasma membrane of these parasites...
  48. ncbi request reprint Radical cure of experimental cutaneous leishmaniasis by the bisphosphonate pamidronate
    Noris Rodriguez
    Laboratory of Molecular Parasitology, Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Infect Dis 186:138-40. 2002
    ..Pamidronate is, therefore, a new lead compound for the synthesis of drugs effective against cutaneous leishmaniasis...
  49. ncbi request reprint Magic-angle spinning (31)P NMR spectroscopy of condensed phosphates in parasitic protozoa: visualizing the invisible
    Benjamin Moreno
    Departments of Chemistry and Biophysics, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA
    FEBS Lett 523:207-12. 2002
    ..These results show that all three parasites contain large stores of condensed phosphates which can be visualized by using magic-angle spinning NMR techniques...
  50. ncbi request reprint Trypanosoma cruzi H+-ATPase 1 (TcHA1) and 2 (TcHA2) genes complement yeast mutants defective in H+ pumps and encode plasma membrane P-type H+-ATPases with different enzymatic properties
    Shuhong Luo
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, 61802, USA
    J Biol Chem 277:44497-506. 2002
    ..cruzi life cycle...
  51. ncbi request reprint Trypanosoma brucei plasma membrane-type Ca(2+)-ATPase 1 (TbPMC1) and 2 (TbPMC2) genes encode functional Ca(2+)-ATPases localized to the acidocalcisomes and plasma membrane, and essential for Ca(2+) homeostasis and growth
    Shuhong Luo
    Department of Pathobiology, and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:14427-39. 2004
    ..These results establish that T. brucei PMCA-Ca(2+)-ATPases are essential for parasite viability and validate them as targets for drug development...
  52. ncbi request reprint Dynamics of polymorphism of acidocalcisomes in Leishmania parasites
    Kildare Miranda
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av Brigadeiro Trompovski, S N, Bloco G, Cidade Universitaria, CEP 21949 900, Rio de Janeiro, Brazil
    Histochem Cell Biol 121:407-18. 2004
    ..X-ray microanalysis and elemental mapping of these structures revealed the additional presence of iron. Together, the results reveal that the morphology and composition of acidocalcisomes are greatly influenced by the culture conditions...
  53. ncbi request reprint Bisphosphonate inhibition of the exopolyphosphatase activity of the Trypanosoma brucei soluble vacuolar pyrophosphatase
    Evangelia Kotsikorou
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    J Med Chem 48:6128-39. 2005
    ..brucei infection. The most active compound in vivo provided a 40% protection from death with no apparent side effects, suggesting that further development of such compounds may be of interest...
  54. ncbi request reprint Design, synthesis, and biological evaluation of phosphinopeptides against Trypanosoma cruzi targeting trypanothione biosynthesis
    Esteban L Ravaschino
    Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina
    J Med Chem 49:426-35. 2006
    ..The simple phosphinopeptide structure found as a pharmacophore in the present study constitutes a starting point for the development of straightforward optimized drugs...
  55. pmc Polyphosphate modulates blood coagulation and fibrinolysis
    Stephanie A Smith
    Department of Biochemistry, College of Medicine, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 103:903-8. 2006
    ....
  56. ncbi request reprint Ammonium production during hypo-osmotic stress leads to alkalinization of acidocalcisomes and cytosolic acidification in Trypanosoma cruzi
    Peter Rohloff
    Department of Pathobiology and Medical Scholars Program, University of Illinos at Urbana Champaign, Urbana, IL, USA
    Mol Biochem Parasitol 150:249-55. 2006
    ....
  57. pmc A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruzi
    Peter Rohloff
    Department of Pathobiology and Medical Scholars Program, University of Illinios at Urbana Champaign, Urbana, IL 61801, USA
    Exp Parasitol 118:17-24. 2008
    ..Polyphosphate hydrolysis occurs during hyposmotic stress, probably increasing the osmotic pressure of the contractile vacuole and facilitating water movement...
  58. ncbi request reprint A malaria parasite-encoded vacuolar H(+)-ATPase is targeted to the host erythrocyte
    Norma Marchesini
    Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 280:36841-7. 2005
    ..This constitutes the first report of the presence of a functional enzyme of parasite origin in the plasma membrane of its host...
  59. pmc Synthesis and biological evaluation of 2-alkylaminoethyl-1,1-bisphosphonic acids against Trypanosoma cruzi and Toxoplasma gondii targeting farnesyl diphosphate synthase
    Sergio H Szajnman
    Departamento de Química Orgánica and UMYMFOR CONICET FCEyN, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
    Bioorg Med Chem 16:3283-90. 2008
    ..gondii growth in vitro. As bisphosphonate-containing compounds are FDA-approved drugs for the treatment of bone resorption disorders, their potential low toxicity makes them good candidates to control tropical diseases...
  60. ncbi request reprint P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francai
    Kildare Miranda
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    J Eukaryot Microbiol 52:55-60. 2005
    ..Taken together, our results indicate that P-type ATPases are involved in intracellular Ca(2+) and H(+) transport in promastigotes of P. francai...
  61. pmc Proteomics in Trypanosoma cruzi--localization of novel proteins to various organelles
    Marcela Ferella
    Department of Cell and Molecular Biology CMB, Karolinska Institutet, Stockholm, Sweden
    Proteomics 8:2735-49. 2008
    ..The results demonstrate that the use of enriched subcellular fractions allows the detection of T. cruzi proteins that are undetected by whole cell proteomic methods...
  62. ncbi request reprint Introduction: A special issue on cellular biology of protozoan parasites
    Roberto Docampo
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research University of Illinois at Urbana Champaign, USA
    Microsc Microanal 10:511-2. 2004
  63. ncbi request reprint Acidocalcisomes and the contractile vacuole complex are involved in osmoregulation in Trypanosoma cruzi
    Peter Rohloff
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:52270-81. 2004
    ..Taken together, these results strongly support a role for acidocalcisomes and the contractile vacuole complex in osmoregulation and identify a functional role for aquaporin in protozoal osmoregulation...
  64. ncbi request reprint Human platelet dense granules contain polyphosphate and are similar to acidocalcisomes of bacteria and unicellular eukaryotes
    Felix A Ruiz
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:44250-7. 2004
    ..The results suggest that acidocalcisomes have been conserved during evolution from bacteria to humans...
  65. ncbi request reprint A functional aquaporin co-localizes with the vacuolar proton pyrophosphatase to acidocalcisomes and the contractile vacuole complex of Trypanosoma cruzi
    Andrea Montalvetti
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 279:38673-82. 2004
    ..Taking together, the presence of an aquaporin in acidocalcisomes and the contractile vacuole complex of T. cruzi, provides support for the role of these organelles in osmotic adaptations of these parasites...
  66. pmc Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi
    Mauricio Vieira
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, IL 61802, U S A
    Biochem J 392:467-74. 2005
    ..cruzi is acidified by a P-type H+-ATPase...

Research Grants21

  1. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2010
    ..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
  2. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2010
    ..The polyphosphate and acidocalcisome metabolic pathways may be good targets for new trypanocidal agents and this work is designed to test the function and significance of polyphosphate and acidocalcisomes of T. brucei. ..
  3. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2003
    ..cruzi, especially the role of acidocalcisomes and the contractile vacuole; Specific aim 2, Investigation of the role of acidocalcisome polyP in osmoregulation. ..
  4. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2006
    ..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
  5. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2007
    ..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
  6. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2009
    ..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
  7. The role of polyphosphate and acidocalcisomes in Trypanosoma brucei
    Roberto Docampo; Fiscal Year: 2009
    ..The polyphosphate and acidocalcisome metabolic pathways may be good targets for new trypanocidal agents and this work is designed to test the function and significance of polyphosphate and acidocalcisomes of T. brucei. ..
  8. The contractile vacuole of Trypanosoma cruzi
    Roberto Docampo; Fiscal Year: 2009
    ..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
  9. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2002
    ..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
  10. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2001
    ..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
  11. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2001
    ..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
  12. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 1999
    ..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
  13. MODE OF ACTION TRYPANOCIDAL DRUGS--INVOLVEMENT OF CA++
    Roberto Docampo; Fiscal Year: 1993
    ....
  14. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2000
    ..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..