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Genomes and Genes | Roberto DocampoSummaryAffiliation: University of Georgia Country: USA Publications
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Acidocalcisomes - conserved from bacteria to manRoberto Docampo
Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, Georgia 30602, USA
Nat Rev Microbiol 3:251-61. 2005..Here, we review acidocalcisome ultrastructure, composition and function in different trypanosomatids and other organisms...
AcidocalcisomesRoberto Docampo
Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens 30602, USA
Cell Calcium 50:113-9. 2011..Acidocalcisomes interact with other organelles in a number of organisms suggesting their association with the endosomal/lysosomal pathway, and are considered part of the lysosome-related group of organelles...- The role of acidocalcisomes in the stress response of Trypanosoma cruziRoberto Docampo
Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA, USA
Adv Parasitol 75:307-24. 2011..This pathway is an excellent target for chemotherapy as shown by the effects of phosphodiesterase C inhibitors on parasite survival... - Calcium- and polyphosphate-containing acidic granules of sea urchin eggs are similar to acidocalcisomes, but are not the targets for NAADPIsabela B Ramos
Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, 30602, USA
Biochem J 429:485-95. 2010.... - Hyperosmotic stress induces aquaporin-dependent cell shrinkage, polyphosphate synthesis, amino acid accumulation, and global gene expression changes in Trypanosoma cruziZhu Hong Li
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
J Biol Chem 286:43959-71. 2011.... 
Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolkIsabela B Ramos
Department of Cellular Biology and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
Biol Cell 102:421-34. 2010....- Adaptor protein-3 (AP-3) complex mediates the biogenesis of acidocalcisomes and is essential for growth and virulence of Trypanosoma bruceiGuozhong Huang
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
J Biol Chem 286:36619-30. 2011..Similar results were obtained by knocking down the expression of the δ subunit of AP-3. These results indicate that AP-3 is essential for the biogenesis of acidocalcisomes and for growth and virulence of T. brucei... - Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbesRoberto Docampo
Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, GA 30602, USA
Philos Trans R Soc Lond B Biol Sci 365:775-84. 2010..Storage of phosphate and cations may represent the ancestral physiological function of acidocalcisomes, with cation and pH homeostasis and osmoregulatory functions derived following the divergence of prokaryotes and eukaryotes... - The acidocalcisome as a target for chemotherapeutic agents in protozoan parasitesRoberto Docampo
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
Curr Pharm Des 14:882-8. 2008..The mechanism of action of bisphosphonates, however, is by inhibition of the isoprenoid pathway and more specifically the prenyl diphosphate synthases... - The role of acidocalcisomes in parasitic protistsSilvia N J Moreno
Department of Cellular Biology and Center for Tropical and Global Emerging Diseases, University of Georgia, Athens, 30602, USA
J Eukaryot Microbiol 56:208-13. 2009..g. the contractile vacuole in Trypanosoma cruzi, and other vacuoles observed in Toxoplasma gondii, suggests that these cellular compartments may be associated with the endosomal/lysosomal pathway... - Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulationJianmin Fang
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Paul D Coverdell Biomedical and Health Sciences Center, Athens, Georgia 30602, USA
J Biol Chem 282:32501-10. 2007..This was accompanied by a delayed regulatory volume decrease after hyposmotic stress. These results support the role of poly P in T. cruzi osmoregulation... - Identification of contractile vacuole proteins in Trypanosoma cruziPaul N Ulrich
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
PLoS ONE 6:e18013. 2011..The CV localization of the proteins investigated revealed potential novel roles of these organelles in phosphate metabolism and provided information on the potential participation of adaptor protein complexes in their biogenesis... - A 43-nucleotide U-rich element in 3'-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotesZhu Hong Li
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
J Biol Chem 287:19058-69. 2012..These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage... - Ablation of a small transmembrane protein of Trypanosoma brucei (TbVTC1) involved in the synthesis of polyphosphate alters acidocalcisome biogenesis and function, and leads to a cytokinesis defectJianmin Fang
Center for Tropical and Emerging Global Disease and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
Biochem J 407:161-70. 2007..A decrease in the poly P content would lead to osmotic sensitivity and defects in cytokinesis... - Acylation-dependent export of Trypanosoma cruzi phosphoinositide-specific phospholipase C to the outer surface of amastigotesVicente de Paulo Martins
Department of Cellular Biology, University of Georgia, Center for Tropical and Emerging Global Diseases, Athens, Georgia 30602, USA
J Biol Chem 285:30906-17. 2010..Taken together, these results indicate that N-terminal acyl modifications serve as a molecular addressing system for sending TcPI-PLC to the outer surface of the cell... - Molecular and electrophysiological characterization of a novel cation channel of Trypanosoma cruziVeronica Jimenez
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, United States of America
PLoS Pathog 8:e1002750. 2012..cruzi after recombinant expression in bacteria. The peculiar characteristics of TcCat could be important for the development of specific inhibitors with therapeutic potential against trypanosomes... - Phosphonium lipocations as antiparasitic agentsTimothy E Long
Department of Pharmaceutical and Biomedical Sciences and Center for Drug Discovery, University of Georgia, Athens, GA 30602, USA
Bioorg Med Chem Lett 22:2976-9. 2012..Optimal phthalimides and 1,4-naphthoquinone-based lipocations were active in vitro at mid-high nM concentrations against P. falciparum and low μM concentrations against T. cruzi... - Trypanosomes and the solution to a 50-year mitochondrial calcium mysteryRoberto Docampo
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30620, USA
Trends Parasitol 28:31-7. 2012..These results add another success story to the already substantial contributions of trypanosomes to mammalian biochemistry... - Developmental expression of a Trypanosoma cruzi phosphoinositide-specific phospholipase C in amastigotes and stimulation of host phosphoinositide hydrolysisVicente de Paulo Martins
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602 2607, USA
Infect Immun 78:4206-12. 2010..The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction... - Chemical validation of phosphodiesterase C as a chemotherapeutic target in Trypanosoma cruzi, the etiological agent of Chagas' diseaseSharon King-Keller
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, 350B Paul D Coverdell Center, 500 D W Brooks Dr, Athens, GA 30602, USA
Antimicrob Agents Chemother 54:3738-45. 2010..The compounds found could be useful tools in the study of osmoregulation in T. cruzi. In addition, their further optimization could result in the development of new drugs against Chagas' disease and other trypanosomiases... - Special section on novel organelles in parasitic protozoaRoberto Docampo
Center for Tropical and Emerging Global Diseases, Paul D. Coverdell Center for Biomedical and Health Sciences, 500 DW Brooks Drive, Athens, GA 30606, USA
Exp Parasitol 118:1. 2008 - Molecular characterization of Trypanosoma brucei P-type H+-ATPasesShuhong Luo
Center for Tropical and Emerging Global Diseases and the Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA
J Biol Chem 281:21963-73. 2006..No evidence was found of an intracellular P-type H+-ATPase activity. These results establish that T. brucei H+-ATPases are plasma membrane enzymes essential for parasite viability... - A pyrophosphatase regulating polyphosphate metabolism in acidocalcisomes is essential for Trypanosoma brucei virulence in miceGuillaume Lemercier
Laboratoire de Genomique Fonctionnelle des Trypanosomatides, UMR CNRS 5162, 146, rue Leo Saignat, 33076 Bordeaux, France
J Biol Chem 279:3420-5. 2004..BFiVSP1 did not cause acute virulent infection in mice, demonstrating that TbVSP1 is essential for growth of bloodstream forms in the mammalian host... - Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruziMaria Laura Salto
Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, IL 61802, United States
Mol Biochem Parasitol 158:120-30. 2008..Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition... - Polyphosphate content and fine structure of acidocalcisomes of Plasmodium falciparumFelix A Ruiz
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA
Microsc Microanal 10:563-7. 2004..Taken together, these results provide evidence that acidocalcisomes from Plasmodium falciparum do not differ significantly from acidocalcisomes of other apicomplexan parasites... - Synthesis and biological evaluation of 1-amino-1,1-bisphosphonates derived from fatty acids against Trypanosoma cruzi targeting farnesyl pyrophosphate synthaseSergio H Szajnman
, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, , Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Bioorg Med Chem Lett 15:4685-90. 2005..cruzi farnesyl pyrophosphate synthase. As bisphosphonate-containing drugs are FDA-approved for the treatment of bone resorption disorders, their potential innocuousness makes them good candidates to control tropical diseases... - Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideumNorma Marchesini
Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 277:8146-53. 2002..Together, these results suggest that the mass-dense granules of D. discoideum are homologous to the acidocalcisomes described in protozoan parasites and are linked to the function of the contractile vacuole... - Calcium regulation in protozoan parasitesSilvia N J Moreno
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, IL 61802, USA
Curr Opin Microbiol 6:359-64. 2003..Recent findings have shed light on the function of these transporters, the roles that they play in cellular metabolism and their potential use for targeting them for new therapies... - The H(+)-pyrophosphatase of Rhodospirillum rubrum is predominantly located in polyphosphate-rich acidocalcisomesManfredo Seufferheld
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 279:51193-202. 2004..Taken together, these results suggest that the H(+)-PPase of R. rubrum has two distinct roles depending on its location acting as an intracellular proton pump in acidocalcisomes but in PPi synthesis in the chromatophore membranes... - Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotesManfredo Seufferheld
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
J Biol Chem 278:29971-8. 2003..The results suggest that acidocalcisomes arose before the prokaryotic and eukaryotic lineages diverged... - An acidocalcisomal exopolyphosphatase from Leishmania major with high affinity for short chain polyphosphateClaudia O Rodrigues
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 277:50899-906. 2002..4 +/- 3.0 mm) and long chain polyP (55.9 +/- 5.6 mm) were detected in L. major promastigotes. The unique characteristics of LmPPX and L. major polyP metabolism may facilitate the development of novel antileishmanial agents... - Acidocalcisomes of Phytomonas françai possess distinct morphological characteristics and contain ironKildare Miranda
, , Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompovski, s/n, Bloco G, , 21949-900, Rio de Janeiro, RJ, Brazil
Microsc Microanal 10:647-55. 2004..Together, these results suggest that the electron-dense organelles of P. françai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content... - Acidocalcisomes of trypanosomatids have species-specific elemental compositionKildare Miranda
, , Univesidade Federal do Rio de Janeiro, Av Brigadeiro Trompovski, s/n, Bloco G, , 21949-900, Rio de Janeiro, RJ, Brazil
Protist 155:395-405. 2004..Taken together, the results strongly indicate a species-specific composition of acidocalcisomes in trypanosomatid parasites... - Trypanosoma cruzi H+-ATPase 1 (TcHA1) and 2 (TcHA2) genes complement yeast mutants defective in H+ pumps and encode plasma membrane P-type H+-ATPases with different enzymatic propertiesShuhong Luo
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, 61802, USA
J Biol Chem 277:44497-506. 2002..cruzi life cycle... - Bisphosphonates derived from fatty acids are potent inhibitors of Trypanosoma cruzi farnesyl pyrophosphate synthaseSergio H Szajnman
, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Bioorg Med Chem Lett 13:3231-5. 2003..As bisphosphonates are FDA clinically approved for treatment of bone resorption disorders, their potential innocuousness makes them good candidates to control tropical diseases... - A plasma membrane P-type H(+)-ATPase regulates intracellular pH in Leishmania mexicana amazonensisNorma Marchesini
Laboratory of Molecular Parasitology, Department of Pathobiology, College of Verterinary Medicine, University of Illinois at Urbana Champaign, 2001 South, Lincoln Avenue, Urbana, IL 61802, USA
Mol Biochem Parasitol 119:225-36. 2002..80+/-0.03 to 7.25+/-0.09 and induce membrane hyperpolarization. No evidence was found of the possible involvement of a K(+)/H(+)-ATPase in intracellular pH regulation in both developmental stages of L. m. amazonensis... - Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigoteMaria Laura Salto
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, 61802, USA
Eukaryot Cell 2:756-68. 2003..Our data suggest that inositolphosphoceramide is implicated in T. cruzi differentiation and that its metabolism could provide important targets for the development of antiparasitic therapies... - In vivo activities of farnesyl pyrophosphate synthase inhibitors against Leishmania donovani and Toxoplasma gondiiVanessa Yardley
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
Antimicrob Agents Chemother 46:929-31. 2002..donovani by intravenous administration. Risedronate had a 50% effective dosage of five 2.6-mg/kg of body weight intraperitoneal doses against L. donovani-infected mice but was less effective against T. gondii-infected mice... - Significant differences between procyclic and bloodstream forms of Trypanosoma brucei in the maintenance of their plasma membrane potentialNicole Van Der Heyden
Department of Pathobiology, University of Illinois at Urbana-Champaign, 61802, USA
J Eukaryot Microbiol 49:407-13. 2002..Thus, there are significant differences between procyclic and bloodstream forms in the maintenance of the deltapsi and in their permeability to cations... - Proton and sodium pumps regulate the plasma membrane potential of different stages of Trypanosoma cruziNicole Van Der Heyden
Laboratory of Molecular Parasitology, Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
Mol Biochem Parasitol 120:127-39. 2002..The presence of K(+) channels in amastigotes and a sodium pump in trypomastigotes, in addition to the H(+)-ATPase, could provide important new targets for trypanocidal drug development... - Magic-angle spinning (31)P NMR spectroscopy of condensed phosphates in parasitic protozoa: visualizing the invisibleBenjamin Moreno
Departments of Chemistry and Biophysics, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA
FEBS Lett 523:207-12. 2002..These results show that all three parasites contain large stores of condensed phosphates which can be visualized by using magic-angle spinning NMR techniques... - Radical cure of experimental cutaneous leishmaniasis by the bisphosphonate pamidronateNoris Rodriguez
Laboratory of Molecular Parasitology, Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Infect Dis 186:138-40. 2002..Pamidronate is, therefore, a new lead compound for the synthesis of drugs effective against cutaneous leishmaniasis... - A proton pumping pyrophosphatase in the Golgi apparatus and plasma membrane vesicles of Trypanosoma cruziRosa Martinez
Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
Mol Biochem Parasitol 120:205-13. 2002..Taken together, these results are consistent with the presence of a functional H(+)-PPase in the plasma membrane of these parasites... - Trypanosoma brucei plasma membrane-type Ca(2+)-ATPase 1 (TbPMC1) and 2 (TbPMC2) genes encode functional Ca(2+)-ATPases localized to the acidocalcisomes and plasma membrane, and essential for Ca(2+) homeostasis and growthShuhong Luo
Department of Pathobiology, and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
J Biol Chem 279:14427-39. 2004..These results establish that T. brucei PMCA-Ca(2+)-ATPases are essential for parasite viability and validate them as targets for drug development... - Dynamics of polymorphism of acidocalcisomes in Leishmania parasitesKildare Miranda
, , Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompovski, s/n, Bloco G, Cidade Universitaria, CEP 21949-900, Rio de Janeiro, Brazil
Histochem Cell Biol 121:407-18. 2004..X-ray microanalysis and elemental mapping of these structures revealed the additional presence of iron. Together, the results reveal that the morphology and composition of acidocalcisomes are greatly influenced by the culture conditions... - A malaria parasite-encoded vacuolar H(+)-ATPase is targeted to the host erythrocyteNorma Marchesini
Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 280:36841-7. 2005..This constitutes the first report of the presence of a functional enzyme of parasite origin in the plasma membrane of its host... - Bisphosphonate inhibition of the exopolyphosphatase activity of the Trypanosoma brucei soluble vacuolar pyrophosphataseEvangelia Kotsikorou
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
J Med Chem 48:6128-39. 2005..brucei infection. The most active compound in vivo provided a 40% protection from death with no apparent side effects, suggesting that further development of such compounds may be of interest... - Design, synthesis, and biological evaluation of phosphinopeptides against Trypanosoma cruzi targeting trypanothione biosynthesisEsteban L Ravaschino
, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, , Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina
J Med Chem 49:426-35. 2006..The simple phosphinopeptide structure found as a pharmacophore in the present study constitutes a starting point for the development of straightforward optimized drugs... - Polyphosphate modulates blood coagulation and fibrinolysisStephanie A Smith
Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 103:903-8. 2006.... - Introduction: A special issue on cellular biology of protozoan parasitesRoberto Docampo
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research University of Illinois at Urbana-Champaign, USA
Microsc Microanal 10:511-2. 2004 - Ammonium production during hypo-osmotic stress leads to alkalinization of acidocalcisomes and cytosolic acidification in Trypanosoma cruziPeter Rohloff
Department of Pathobiology and Medical Scholars Program, University of Illinos at Urbana-Champaign, Urbana, IL, USA
Mol Biochem Parasitol 150:249-55. 2006.... - A contractile vacuole complex is involved in osmoregulation in Trypanosoma cruziPeter Rohloff
Department of Pathobiology and Medical Scholars Program, University of Illinios at Urbana Champaign, Urbana, IL 61801, USA
Exp Parasitol 118:17-24. 2008..Polyphosphate hydrolysis occurs during hyposmotic stress, probably increasing the osmotic pressure of the contractile vacuole and facilitating water movement... - Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruziMauricio Vieira
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, IL 61802, U.S.A
Biochem J 392:467-74. 2005..cruzi is acidified by a P-type H+-ATPase... - Acidocalcisomes and the contractile vacuole complex are involved in osmoregulation in Trypanosoma cruziPeter Rohloff
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 279:52270-81. 2004..Taken together, these results strongly support a role for acidocalcisomes and the contractile vacuole complex in osmoregulation and identify a functional role for aquaporin in protozoal osmoregulation... - Synthesis and biological evaluation of 2-alkylaminoethyl-1,1-bisphosphonic acids against Trypanosoma cruzi and Toxoplasma gondii targeting farnesyl diphosphate synthaseSergio H Szajnman
Departamento de Química Orgánica and UMYMFOR CONICET FCEyN, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Bioorg Med Chem 16:3283-90. 2008..gondii growth in vitro. As bisphosphonate-containing compounds are FDA-approved drugs for the treatment of bone resorption disorders, their potential low toxicity makes them good candidates to control tropical diseases... - Human platelet dense granules contain polyphosphate and are similar to acidocalcisomes of bacteria and unicellular eukaryotesFelix A Ruiz
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 279:44250-7. 2004..The results suggest that acidocalcisomes have been conserved during evolution from bacteria to humans... - A functional aquaporin co-localizes with the vacuolar proton pyrophosphatase to acidocalcisomes and the contractile vacuole complex of Trypanosoma cruziAndrea Montalvetti
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
J Biol Chem 279:38673-82. 2004..Taking together, the presence of an aquaporin in acidocalcisomes and the contractile vacuole complex of T. cruzi, provides support for the role of these organelles in osmotic adaptations of these parasites... - Proteomics in Trypanosoma cruzi--localization of novel proteins to various organellesMarcela Ferella
Department of Cell and Molecular Biology CMB, Karolinska Institutet, Stockholm, Sweden
Proteomics 8:2735-49. 2008..The results demonstrate that the use of enriched subcellular fractions allows the detection of T. cruzi proteins that are undetected by whole cell proteomic methods... - P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francaiKildare Miranda
, , Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
J Eukaryot Microbiol 52:55-60. 2005..Taken together, our results indicate that P-type ATPases are involved in intracellular Ca(2+) and H(+) transport in promastigotes of P. francai...
Research Grants
- The contractile vacuole of Trypanosoma cruziRoberto Docampo; Fiscal Year: 2010..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
- The role of polyphosphate and acidocalcisomes in Trypanosoma bruceiRoberto Docampo; Fiscal Year: 2010..The polyphosphate and acidocalcisome metabolic pathways may be good targets for new trypanocidal agents and this work is designed to test the function and significance of polyphosphate and acidocalcisomes of T. brucei. ..
- ACIDOCALCISOMES IN TRYPANOSOMA CRUZIRoberto Docampo; Fiscal Year: 2003..cruzi, especially the role of acidocalcisomes and the contractile vacuole; Specific aim 2, Investigation of the role of acidocalcisome polyP in osmoregulation. ..
- The contractile vacuole of Trypanosoma cruziRoberto Docampo; Fiscal Year: 2007..cruzi. Specific aim 2: To identify the molecular mechanism involved in the traffic T. cruzi aquaporin to the contractile vacuole complex and the role of aquaporin in regulatory volume decrease. ..
- ACIDOCALCISOMES IN TRYPANOSOMA CRUZIRoberto Docampo; Fiscal Year: 2002..cruzi H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
- MODE OF ACTION TRYPANOCIDAL DRUGS--INVOLVEMENT OF CA++Roberto Docampo; Fiscal Year: 1993....