Michelle A Digman

Summary

Affiliation: University of Illinois
Country: USA

Publications

  1. ncbi request reprint Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode
    Rooshin B Dalal
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Microsc Res Tech 71:69-81. 2008
  2. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
  3. pmc Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 88:L33-6. 2005
  4. pmc Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 102:691-7. 2012
  5. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008
  6. pmc The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 100:1829-36. 2011
  7. pmc Stoichiometry of molecular complexes at adhesions in living cells
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering and Development Biology Center Optical Biology Core Facility, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 106:2170-5. 2009
  8. pmc In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 107:16560-5. 2010
  9. pmc Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92617, USA
    Proc Natl Acad Sci U S A 110:135-40. 2013
  10. pmc Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Microsc Res Tech 75:271-81. 2012

Collaborators

  • Enrico Gratton
  • Francesco Cardarelli
  • Klaus M Hahn
  • Hector Giral
  • C M Brown
  • N Sidenius
  • Makoto Inoue
  • Elizabeth Hinde
  • Luca Lanzano
  • Giulia Ossato
  • Rooshin B Dalal
  • Valeria R Caiolfa
  • Wonhwa Cho
  • Robert V Stahelin
  • Nikhil A Gokhale
  • Maria Luisa Barcellona
  • Christopher Welch
  • Moshe Levi
  • Judith Kimble
  • Peter Fwu
  • Aaron Kershner
  • J Lawrence Marsh
  • Tamas Lukacsovich
  • Charity Aiken
  • Alan F Horwitz
  • Valeria Vetri
  • Moreno Zamai
  • Chris D Madsen
  • Francesco Blasi
  • Gabriele Malengo
  • Jason Sutin
  • Annapaola Andolfo
  • John D Rafter
  • Martina Medkova
  • Heather R Melowic
  • Alexandra Abraham
  • Bharath Ananthanarayanan
  • Theodore Hazlett
  • Seth Gammon

Detail Information

Publications24

  1. ncbi request reprint Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode
    Rooshin B Dalal
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Microsc Res Tech 71:69-81. 2008
    ..Furthermore, we distinguish between mobile and immobile components, and introduce a method to correct for slow variations in intensity...
  2. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
    ..This new type of data analysis has a broad application in biology and it provides a powerful tool for measuring fast as well as slower dynamic processes in cellular systems using any standard laser confocal microscope...
  3. pmc Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 88:L33-6. 2005
    ..We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale...
  4. pmc Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 102:691-7. 2012
    ..For the first time, to our knowledge, we were able to probe chromatin compaction on the micrometer scale, where the regulation of molecular diffusion may become relevant for many cellular processes...
  5. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008
    ....
  6. pmc The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 100:1829-36. 2011
    ..These two distinct routes of molecular flow were concomitantly measured in the Caenorhabditis elegans germ line, which indicates a conservation of mechanism on a scale more widespread than cell type or organism...
  7. pmc Stoichiometry of molecular complexes at adhesions in living cells
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering and Development Biology Center Optical Biology Core Facility, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 106:2170-5. 2009
    ..These aggregates disassemble rapidly in the cytoplasm because large complexes are found only in very close proximity to the adhesions or at their borders...
  8. pmc In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 107:16560-5. 2010
    ..This is a unique in vivo demonstration of the intricate chromatin network showing channel directed diffusion of an inert molecule with high spatial and temporal resolution...
  9. pmc Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92617, USA
    Proc Natl Acad Sci U S A 110:135-40. 2013
    ..This multiplexed approach to biosensor FRET detection serves as a unique tool for dissection of the mechanism(s) by which key signaling proteins are spatially and temporally coordinated...
  10. pmc Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy
    Elizabeth Hinde
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Microsc Res Tech 75:271-81. 2012
    ....
  11. pmc Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2819-31. 2008
    ....
  12. pmc Lessons in fluctuation correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92697, USA
    Annu Rev Phys Chem 62:645-68. 2011
    ..Image fluctuation analysis has become a rich and powerful technique that can be used to extract information about the spatial distribution of molecular concentration and transport in cells and tissues...
  13. pmc Scanning image correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine, CA, USA
    Bioessays 34:377-85. 2012
    ..These methods can be implemented with a standard laser scanning microscope and produce a cellular level spatio-temporal map of molecular interactions...
  14. pmc Nanometer-scale imaging by the modulation tracking method
    Luca Lanzano
    Laboratory for Fluorescence Dynamics, University of California Biomedical Engineering, 3210 Natural Sciences 2, Irvine, California 92672, USA
    J Biophotonics 4:415-24. 2011
    ..Our approach to super-resolution and to 3D nanoimaging is different than other proposed methods that break the diffraction limit using non-linear effects or are based on single molecule localization...
  15. pmc A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis
    Giulia Ossato
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 98:3078-85. 2010
    ..The behavior of Httex1p in COS-7 and ST14A cells is compared...
  16. pmc Imaging barriers to diffusion by pair correlation functions
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 97:665-73. 2009
    ..It does not require isolated molecules, and thus many molecules can be labeled at the same time and within the point spread function...
  17. pmc Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
    Wiley Interdiscip Rev Syst Biol Med 1:273-82. 2009
    ..Although one example of the application of the fluctuation method is given, this article also contains simulations that are better suited to illustrate and support the basic assumptions of the method...
  18. doi request reprint Raster image correlation spectroscopy and number and brightness analysis
    Michelle A Digman
    Department of Biomedical Engineering, University of California, Irvine, California, USA
    Methods Enzymol 518:121-44. 2013
    ..We also discuss common control experiments needed to rule out instrumental artifacts and how to calibrate the microscope in terms of relative molecular brightness...
  19. pmc The phasor approach to fluorescence lifetime imaging analysis
    Michelle A Digman
    Biophys J 94:L14-6. 2008
    ....
  20. pmc Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
    Valeria R Caiolfa
    Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 179:1067-82. 2007
    ..These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents...
  21. ncbi request reprint Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer
    Nikhil A Gokhale
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607 7061, USA
    J Biol Chem 280:42831-40. 2005
    ..Collectively, these studies elucidate the structural determinant of the PtdIns(4,5)P2 selectivity of A2t and suggest that A2t may be involved in the regulation of PtdIns(4,5)P2 clustering in the cell...
  22. ncbi request reprint Diacylglycerol-induced membrane targeting and activation of protein kinase Cepsilon: mechanistic differences between protein kinases Cdelta and Cepsilon
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, 60607, USA
    J Biol Chem 280:19784-93. 2005
    ..Collectively, these results provide new insight into the differential activation mechanisms of PKCdelta and PKCepsilon based on different structural and functional properties of their C1 domains...
  23. ncbi request reprint Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes
    Maria Luisa Barcellona
    Department of Biological Chemistry and Molecular Biology, University of Catania, Catania, Italy
    Microsc Res Tech 65:205-17. 2004
    ....
  24. ncbi request reprint Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity
    Makoto Inoue
    Department of Medicine, University of Colorado Health Sciences Center and Denver Veterans Affairs Medical Center, Denver, Colorado 80262, USA
    J Biol Chem 279:49160-71. 2004
    ....