R C Dickson

Summary

Affiliation: University of Kentucky
Country: USA

Publications

  1. ncbi Synthesis of mannose-(inositol-P)2-ceramide, the major sphingolipid in Saccharomyces cerevisiae, requires the IPT1 (YDR072c) gene
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:29620-5. 1997
  2. ncbi Metabolism and selected functions of sphingolipids in the yeast Saccharomyces cerevisiae
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, KY 40536 0298, USA
    Biochim Biophys Acta 1438:305-21. 1999
  3. ncbi Yeast sphingolipids
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, KY 40536 0084, USA
    Biochim Biophys Acta 1426:347-57. 1999
  4. ncbi Sphingolipid functions in Saccharomyces cerevisiae: comparison to mammals
    R C Dickson
    Department of Biochemistry, University of Kentucky College of Medicine, Lexington, USA
    Annu Rev Biochem 67:27-48. 1998
  5. ncbi Sphingolipids are potential heat stress signals in Saccharomyces
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:30196-200. 1997
  6. ncbi Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene
    M M Nagiec
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:9809-17. 1997
  7. ncbi The LCB4 (YOR171c) and LCB5 (YLR260w) genes of Saccharomyces encode sphingoid long chain base kinases
    M M Nagiec
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 273:19437-42. 1998
  8. pmc Suppressor gene analysis reveals an essential role for sphingolipids in transport of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae
    M Skrzypek
    Department of Biochemistry, Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington 40536 0084, USA
    J Bacteriol 179:1513-20. 1997
  9. ncbi Identification of a Saccharomyces gene, LCB3, necessary for incorporation of exogenous long chain bases into sphingolipids
    L Qie
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:16110-7. 1997
  10. ncbi Elevation of endogenous sphingolipid long-chain base phosphates kills Saccharomyces cerevisiae cells
    X Zhang
    Department of Molecular and Cellular Biochemistry and Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington 40536, USA
    Curr Genet 40:221-33. 2001

Collaborators

  • R L Lester
  • X Zhang
  • K Liu
  • STEPHEN STURLEY
  • Katsuhide Fujita
  • M M Nagiec
  • M Skrzypek
  • Guangzuo Luo
  • Jennifer L Brace
  • Martin Valachovic
  • Sabrina Zink
  • Ji Hyun Chung
  • John D Hearn
  • Nitai C Hait
  • Stacey R Ferguson-Yankey
  • Ying Liu
  • M S Skrzypek
  • Ole N Jensen
  • Albrecht Gruhler
  • Charles M Rudin
  • Dina Balderes
  • M Shah Alam Bhuiyan
  • James Eckstein
  • Bart M Bareither
  • Lisa Wilcox
  • Martin Bard
  • Robert Barbuch
  • Constance Mehlgarten
  • Raffael Schaffrath
  • Michael J R Stark
  • Hiroko K Kitamoto
  • Junko Nagase
  • E E Nagiec
  • Daniel Jablonowski
  • J A Baltisberger
  • L Qie
  • Marek S Skrzypek
  • J Shelling
  • N Zingg
  • G B Wells
  • P Spielmann

Detail Information

Publications30

  1. ncbi Synthesis of mannose-(inositol-P)2-ceramide, the major sphingolipid in Saccharomyces cerevisiae, requires the IPT1 (YDR072c) gene
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:29620-5. 1997
    ..Identification of the IPT1 gene should be helpful in determining the function of the M(IP)2C sphingolipid and in determining the catalytic mechanism of IPC and M(IP)2C synthases...
  2. ncbi Metabolism and selected functions of sphingolipids in the yeast Saccharomyces cerevisiae
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, KY 40536 0298, USA
    Biochim Biophys Acta 1438:305-21. 1999
    ..Finally, we discuss recent clues that may help to reveal how sphingolipid synthesis and total cellular sphingolipid content are regulated...
  3. ncbi Yeast sphingolipids
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, KY 40536 0084, USA
    Biochim Biophys Acta 1426:347-57. 1999
    ..This essential step in yeast sphingolipid synthesis, which is not found in humans, appears to be an excellent target for the development of more effective antifungal compounds, both for human and for agricultural use...
  4. ncbi Sphingolipid functions in Saccharomyces cerevisiae: comparison to mammals
    R C Dickson
    Department of Biochemistry, University of Kentucky College of Medicine, Lexington, USA
    Annu Rev Biochem 67:27-48. 1998
    ....
  5. ncbi Sphingolipids are potential heat stress signals in Saccharomyces
    R C Dickson
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:30196-200. 1997
    ..Using genetic and other approaches it should be possible to identify sphingolipid signaling pathways in S. cerevisiae and quantify the importance of each during heat stress...
  6. ncbi Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene
    M M Nagiec
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:9809-17. 1997
    ..Now that IPC synthase has been shown to be the target for aureobasidin A, it should be possible to develop high throughput screens to identify new inhibitors of IPC synthase to combat fungal diseases...
  7. ncbi The LCB4 (YOR171c) and LCB5 (YLR260w) genes of Saccharomyces encode sphingoid long chain base kinases
    M M Nagiec
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 273:19437-42. 1998
    ..cerevisiae. Potential mammalian cDNA homologs of the LCB kinase genes may prove useful in helping to understand the function of sphingosine 1-phosphate in mammals...
  8. pmc Suppressor gene analysis reveals an essential role for sphingolipids in transport of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae
    M Skrzypek
    Department of Biochemistry, Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington 40536 0084, USA
    J Bacteriol 179:1513-20. 1997
    ..Therefore, sphingolipids must be necessary for a step in formation of COPII vesicles or for their transport to the Golgi apparatus. Our data identify the Cwp2 protein as a vital component in protecting cells from the stress of low pH...
  9. ncbi Identification of a Saccharomyces gene, LCB3, necessary for incorporation of exogenous long chain bases into sphingolipids
    L Qie
    Department of Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0084, USA
    J Biol Chem 272:16110-7. 1997
    ..It is the first candidate for such a transporter and the first member of what appears to be a new class of membrane-bound proteins...
  10. ncbi Elevation of endogenous sphingolipid long-chain base phosphates kills Saccharomyces cerevisiae cells
    X Zhang
    Department of Molecular and Cellular Biochemistry and Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington 40536, USA
    Curr Genet 40:221-33. 2001
    ..These and other data indicate the existence of an unknown mechanism(s) for regulating LCB levels. Our results demonstrate that LCBPs may be used in some circumstances to regulate yeast cell growth...
  11. ncbi A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase
    M M Nagiec
    Department of Biochemistry, University of Kentucky Medical Center, Lexington 40536 0084
    J Biol Chem 268:22156-63. 1993
    ....
  12. ncbi Dominant suppressor mutation bypasses the sphingolipid requirement for growth of Saccharomyces cells at low pH: role of the CWP2 gene
    M Skrzypek
    Department of Biochemistry and the Lucille, P Markey Cancer Center, University of Kentucky College of Medicine, Lexington 40536, USA
    Curr Genet 38:191-201. 2000
    ..These results suggest new cellular functions for the Cwp2 protein...
  13. ncbi Signalling functions for sphingolipid long-chain bases in Saccharomyces cerevisiae
    K Liu
    Department of Cellular and Molecular Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington, KY 40536 0298, USA
    Biochem Soc Trans 33:1170-3. 2005
    ..As we learn more about the cellular processes controlled by Ypk1, Ypk2 and Sch9, we will have a far greater appreciation of LCBs as second messengers...
  14. pmc Analysis of phosphorylated sphingolipid long-chain bases reveals potential roles in heat stress and growth control in Saccharomyces
    M S Skrzypek
    Department of Biochemistry and Lucille P Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536 0298, USA
    J Bacteriol 181:1134-40. 1999
    ..Our procedure should expedite experiments to determine how the synthesis and breakdown of these compounds is regulated and how the compounds mediate resistance to elevated temperature...
  15. ncbi High-performance liquid chromatography analysis of molecular species of sphingolipid-related long chain bases and long chain base phosphates in Saccharomyces cerevisiae after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
    R L Lester
    Department of Molecular and Cellular Biochemistry and Lucille P Markey Cancer Center, University of Kentucky College of Medicine, 800 Rose Street, Lexington, Kentucky 40536 0298, USA
    Anal Biochem 298:283-92. 2001
    ..Five molecular species of both sphingolipid long chain bases and their phosphorylated derivatives are for the first time separated and analyzed. The procedure is quite sensitive, requiring only approximately 10(8) wild-type cells...
  16. ncbi Sphingolipid synthesis: identification and characterization of mammalian cDNAs encoding the Lcb2 subunit of serine palmitoyltransferase
    M M Nagiec
    Department of Biochemistry, University of Kentucky Medical Center, Lexington 40536 0084, USA
    Gene 177:237-41. 1996
    ..The mammalian LCB2 cDNAs provide valuable reagents for studying the Lcb2 subunit of SPT and for studying how ceramide synthesis is regulated...
  17. pmc Cumulative mutations affecting sterol biosynthesis in the yeast Saccharomyces cerevisiae result in synthetic lethality that is suppressed by alterations in sphingolipid profiles
    Martin Valachovic
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, 40536
    Genetics 173:1893-908. 2006
    ..We show that deletion of YND1, like deletion of GDA1, alters the sphingolipid profiles, suggesting that changes in sphingolipids compensate for lethality produced by changes in sterol composition and abundance...
  18. ncbi Functions and metabolism of sphingolipids in Saccharomyces cerevisiae
    Robert C Dickson
    Department of Molecular and Cellular Biochemistry and the Lucille Markey Cancer Center, University of Kentucky College of Medicine, Lexington, KY 40536 0509, USA
    Prog Lipid Res 45:447-65. 2006
    ....
  19. pmc SVF1 regulates cell survival by affecting sphingolipid metabolism in Saccharomyces cerevisiae
    Jennifer L Brace
    Johns Hopkins University, Baltimore, Maryland 21231, USA
    Genetics 175:65-76. 2007
    ..Together, these data suggest that compartmentalized generation of distinct intracellular subsets of sphingoid bases may be critical for activation of signaling pathways that control cell growth and survival...
  20. doi More chores for TOR: de novo ceramide synthesis
    Robert C Dickson
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536, USA
    Cell Metab 7:99-100. 2008
    ..This work may provide insight into how chemotherapeutic drugs increase de novo ceramide synthesis and promote cell death in humans...
  21. pmc The sphingolipid long-chain base-Pkh1/2-Ypk1/2 signaling pathway regulates eisosome assembly and turnover
    Guangzuo Luo
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
    J Biol Chem 283:10433-44. 2008
    ..Eisosomes then control endocytosis to align the composition and function of the plasma membrane to match demand...
  22. pmc Mannosyl-diinositolphospho-ceramide, the major yeast plasma membrane sphingolipid, governs toxicity of Kluyveromyces lactis zymocin
    Sabrina Zink
    Biologicum, Institut fur Genetik, Martin Luther Universitat Halle Wittenberg, Weinbergweg 10, D 06120 Halle Saale, Germany
    Eukaryot Cell 4:879-89. 2005
    ..In sum, M(IP)(2)C operates in a plasma membrane step that follows recognition of cell wall chitin by zymocin but precedes the involvement of elongator, the potential toxin target...
  23. ncbi The sphingoid long chain base phytosphingosine activates AGC-type protein kinases in Saccharomyces cerevisiae including Ypk1, Ypk2, and Sch9
    Ke Liu
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, 40536, USA
    J Biol Chem 280:22679-87. 2005
    ..Thus, our data support a model in which PHS, rather than simply being an upstream activator of Pkh1, also activates kinases that are downstream targets of Pkh1 including Ypk1/2 and Sch9...
  24. ncbi Pil1p and Lsp1p negatively regulate the 3-phosphoinositide-dependent protein kinase-like kinase Pkh1p and downstream signaling pathways Pkc1p and Ypk1p
    Xiping Zhang
    Department of Molecular and Cellular Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
    J Biol Chem 279:22030-8. 2004
    ..Homologs of Pil1p and Lsp1p are widespread in nature, and our results suggest that they may be negative regulators of PDK-like protein kinases and their downstream cellular pathways that control cell growth and survival...
  25. ncbi Sphingolipid requirement for generation of a functional v1 component of the vacuolar ATPase
    Ji Hyun Chung
    Department of Molecular and Cellular Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
    J Biol Chem 278:28872-81. 2003
    ..Reciprocal experiments with V0 from sur4Delta cells show that it is normal. We conclude that sphingolipids with a C26 acyl group are required for generating fully functional V1 domains...
  26. ncbi Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes
    Nitai C Hait
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536, USA
    FEBS Lett 532:97-102. 2002
    ..These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded...
  27. ncbi The uracil transporter Fur4p associates with lipid rafts
    John D Hearn
    Department of Molecular and Cellular Biochemistry and Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington 40536, USA
    J Biol Chem 278:3679-86. 2003
    ..Further studies using lcb1-100 cells should reveal new roles of sphingolipids in nutrient uptake and other membrane-dependent processes...
  28. ncbi Sphingolipid functions in Saccharomyces cerevisiae
    Robert C Dickson
    Department of Cellular and Molecular Biochemistry and the Lucille P Markey Cancer Center, University of Kentucky College of Medicine, Lexington 40536 0298, USA
    Biochim Biophys Acta 1583:13-25. 2002
    ..Other studies suggest roles for sphingolipids in exocytosis, growth regulation and longevity. Finally, some progress has been made in understanding how sphingolipid synthesis is regulated and how sphingolipid levels are maintained...
  29. ncbi Mutant analysis reveals complex regulation of sphingolipid long chain base phosphates and long chain bases during heat stress in yeast
    Stacey R Ferguson-Yankey
    Department of Molecular and Cellular Biochemistry, Lucille P Markey Cancer Center, University of Kentucky College of Medicine, 800 Rose Street, Lexington, KY 40536, USA
    Yeast 19:573-86. 2002
    ..Our data provide a basis for determining how the basal and heat-induced levels of individual species of LCBs and LCBPs are governed by the Lcb4p and Lcb5p kinases, the Dpl1p lyase and the Lcb3p phosphatase...
  30. pmc Thematic review series: sphingolipids. New insights into sphingolipid metabolism and function in budding yeast
    Robert C Dickson
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536 0509, USA
    J Lipid Res 49:909-21. 2008
    ..This new knowledge provides a foundation for greatly advancing our understanding of sphingolipid biology in yeast...