Zygmunt Derewenda

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi request reprint On wine, chirality and crystallography
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0736, USA
    Acta Crystallogr A 64:246-58. 2008
  2. doi request reprint Protein crystallization in drug design: towards a rational approach
    Zygmunt S Derewenda
    University of Virginia, Integrated Center for Structure and Function Innovation PSI2, Departments of Molecular Physiology and Biological Physics, PO Box 800736, Jordan Hall, Charlottesville, VA 22908 0736, USA 1 434 243 6842 1 434 982 1616
    Expert Opin Drug Discov 2:1329-40. 2007
  3. pmc On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF
    Meiying Zheng
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, Virginia, 22908 0736, USA
    BMC Struct Biol 9:36. 2009
  4. ncbi request reprint PAF-acetylhydrolases
    Z S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, P O Box 10011, Charlottesville, VA 22906 0011, USA
    Biochim Biophys Acta 1441:229-36. 1999
  5. ncbi request reprint Entropy and surface engineering in protein crystallization
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 229008 0736, USA
    Acta Crystallogr D Biol Crystallogr 62:116-24. 2006
  6. ncbi request reprint The use of recombinant methods and molecular engineering in protein crystallization
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Methods 34:354-63. 2004
  7. ncbi request reprint Advances in Protein Crystallography - fourth annual meeting
    Zygmunt Derewenda
    University of Virginia School of Medicine, Department of Molecular Physiology and Biological Physics, PO Box 800736, Charlottesville, VA 22908 0836, USA
    IDrugs 10:256-8. 2007
  8. ncbi request reprint Rational protein crystallization by mutational surface engineering
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908 USA
    Structure 12:529-35. 2004
  9. pmc It's all in the crystals…
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0793, USA
    Acta Crystallogr D Biol Crystallogr 67:243-8. 2011
  10. pmc Application of protein engineering to enhance crystallizability and improve crystal properties
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 66:604-15. 2010

Research Grants

  1. Preparing high resolution membrane protein crystals
    Zygmunt Derewenda; Fiscal Year: 2005
  2. Molecular Mechanisms of Neuronal Migrations
    Zygmunt S Derewenda; Fiscal Year: 2010
  3. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
  4. Molecular Mechanisms of Neuronal Migration
    Zygmunt Derewenda; Fiscal Year: 2005
  5. MECHANISMS OF LIPID-MEDIATED SIGNAL TRANSDUCTION
    Zygmunt Derewenda; Fiscal Year: 2001
  6. Molecular Mechanisms of Neuronal Migrations
    Zygmunt Derewenda; Fiscal Year: 2009

Collaborators

  • Jacek Otlewski
  • Zbigniew Dauter
  • Miroslawa Dauter
  • Myung Hee Kim
  • A P Somlyo
  • Peter G Vekilov
  • David Sherman
  • Scott A Lesley
  • David S Waugh
  • RONALD RAINES
  • Robert Nakamoto
  • Ian Wilson
  • Meiying Zheng
  • D I Svergun
  • Wladek Minor
  • Z Szewczuk
  • Kyung Jin Kim
  • David Eisenberg
  • Urszula Derewenda
  • David R Cooper
  • Yancho Devedjiev
  • Tomasz Cierpicki
  • Beom Sik Kang
  • Andrzej Joachimiak
  • Jakub Bielnicki
  • Arkadiusz Oleksy
  • Agnieszka Mateja
  • Marcin Paduch
  • John H Bushweller
  • Izabela Janda
  • Cataldo Tarricone
  • Marcin Cieslik
  • Kenton Longenecker
  • Katarzyna Grelewska
  • Katarzyna Smietana
  • Tomasz Boczek
  • Lukasz Goldschmidt
  • David Cooper
  • Jolanta Grembecka
  • Franco Perrina
  • Andrea Musacchio
  • Yogesh Surendranath
  • Jan Czepas
  • Daniel Krowarsch
  • Filip Jelen
  • T W Martin
  • Chang Yub Kim
  • Minmin Yu
  • Erik J Fernandez
  • Jakub Gruszczyk
  • Kelly L Gorres
  • Aming Zhang
  • Marta Kasterka
  • Li Wei Hung
  • Maxim Petoukhov
  • Monika Kasztura
  • Monika Biernat
  • Steve Lukasik
  • Ashutosh Tripathy
  • Won Chan Choi
  • David S Cafiso
  • Malgorzata Pinkowska
  • Piotr Stefanowicz
  • Michal Zawadzki
  • Malgorzata Sikorska
  • Łukasz Opaliński
  • Artem G Evdokimov
  • Ursula Jakob
  • Rong guang Zhang
  • Michael Purdy
  • Justyna Korczynska
  • Alexandra Gabrys
  • Maksymilian Chruszcz
  • Silvia Monzani
  • Aleksandra Gabrys
  • Karen M Routzahn
  • Lour Lezondra
  • Andra S Stevenson
  • Paul C F Graf
  • Stefan Knapp
  • Li Huei Tsai
  • Lucia Massimiliano
  • Holly Barton
  • Paul Read
  • Shin Kai Lin
  • Peter Sheffield
  • Min He

Detail Information

Publications46

  1. ncbi request reprint On wine, chirality and crystallography
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0736, USA
    Acta Crystallogr A 64:246-58. 2008
    ..But one central inanimate character was there at all times - an inconspicuous somewhat obscure organic compound found in wine: tartaric acid. This is the story of its contribution to science...
  2. doi request reprint Protein crystallization in drug design: towards a rational approach
    Zygmunt S Derewenda
    University of Virginia, Integrated Center for Structure and Function Innovation PSI2, Departments of Molecular Physiology and Biological Physics, PO Box 800736, Jordan Hall, Charlottesville, VA 22908 0736, USA 1 434 243 6842 1 434 982 1616
    Expert Opin Drug Discov 2:1329-40. 2007
    ..This is accomplished by creating surface patches readily mediating weak, but specific, intermolecular interactions that take on the role of crystal contacts during nucleation and crystal growth phase...
  3. pmc On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF
    Meiying Zheng
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, Virginia, 22908 0736, USA
    BMC Struct Biol 9:36. 2009
    ..These GEFs include p115, LARG and PDZRhoGEF (PRG)...
  4. ncbi request reprint PAF-acetylhydrolases
    Z S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, P O Box 10011, Charlottesville, VA 22906 0011, USA
    Biochim Biophys Acta 1441:229-36. 1999
    ..Recent crystallographic studies shed new light on the complex structure-function relationships in PAF-AHs...
  5. ncbi request reprint Entropy and surface engineering in protein crystallization
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 229008 0736, USA
    Acta Crystallogr D Biol Crystallogr 62:116-24. 2006
    ....
  6. ncbi request reprint The use of recombinant methods and molecular engineering in protein crystallization
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Methods 34:354-63. 2004
    ..This review discusses recent advances in the field and describes a number of examples of diverse protein engineering techniques used in crystallographic investigations...
  7. ncbi request reprint Advances in Protein Crystallography - fourth annual meeting
    Zygmunt Derewenda
    University of Virginia School of Medicine, Department of Molecular Physiology and Biological Physics, PO Box 800736, Charlottesville, VA 22908 0836, USA
    IDrugs 10:256-8. 2007
  8. ncbi request reprint Rational protein crystallization by mutational surface engineering
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908 USA
    Structure 12:529-35. 2004
    ..Several recent examples of this new methodology suggest that the method has the potential to become a routine tool in protein crystallography...
  9. pmc It's all in the crystals…
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0793, USA
    Acta Crystallogr D Biol Crystallogr 67:243-8. 2011
    ..Here, one such established strategy, surface-entropy reduction (SER), is discussed, including its successes, limitations and possible future developments...
  10. pmc Application of protein engineering to enhance crystallizability and improve crystal properties
    Zygmunt S Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 66:604-15. 2010
    ..This article presents an overview of protein-engineering methods designed to enhance crystallizability and discusses a number of examples of their successful application...
  11. ncbi request reprint The impact of Lys-->Arg surface mutations on the crystallization of the globular domain of RhoGDI
    Jan Czepas
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 60:275-80. 2004
    ..0 A the resolution of the diffraction of the wild-type crystal. Major crystal contacts in the new lattice were created by the mutated epitope...
  12. pmc The role of entropy and polarity in intermolecular contacts in protein crystals
    Marcin Cieslik
    Department of Molecular Physiology and Biological Physics and the PSI2 Integrated Center for Structure Function Innovation, University of Virginia, Charlottesville, VA 22908, USA
    Acta Crystallogr D Biol Crystallogr 65:500-9. 2009
    ..It is also shown that crystal contacts with larger buried surfaces containing eight or more amino acids have cores that are depleted of polar amino acids...
  13. ncbi request reprint Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution
    Kenton Longenecker
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Acta Crystallogr D Biol Crystallogr 59:876-80. 2003
    ..The data support the notion that differences observed between the mutants in vivo are likely to arise from altered affinities for RhoGDI and not from direct structural differences...
  14. pmc Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains
    Meiying Zheng
    Department of Molecular Physiology and Biological Physics, Integrated Center for Structure Function Innovation, University of Virginia, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 65:356-65. 2009
    ..Finally, the TM0439 structure is compared with two other FadR-family structures recently deposited by structural genomics consortia. The results call for a revision in the classification of the FadR family of transcription factors...
  15. pmc Harvesting the high-hanging fruit: the structure of the YdeN gene product from Bacillus subtilis at 1.8 angstroms resolution
    Izabela Janda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 60:1101-7. 2004
    ..8 angstroms resolution. The protein is a canonical alpha/beta hydrolase, with an active site that is accessible to solvent...
  16. ncbi request reprint Structure of the Bacillus subtilis OhrB hydroperoxide-resistance protein in a fully oxidized state
    David R Cooper
    Department of Molecular Physiology and Biological Physics, University of Virginia and the PSI2 Integrated Center for Structure Function Innovation Charlottesville, Virginia 22908, USA
    Acta Crystallogr D Biol Crystallogr 63:1269-73. 2007
    ..However, the stereochemistry of the disulfides is unorthodox and strained, suggesting that they are sensitive to reducing agents. A deep solvent-accessible gorge reaching Cys55 may represent the access route for the reductant...
  17. ncbi request reprint The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution
    Agnieszka Mateja
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Acta Crystallogr D Biol Crystallogr 58:1983-91. 2002
    ....
  18. ncbi request reprint The structure of the FERM domain of merlin, the neurofibromatosis type 2 gene product
    Beom Sik Kang
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 58:381-91. 2002
    ..The structure allows a better rationalization of the impact of selected disease-causing mutations on the integrity of the protein...
  19. pmc Structure and function of Bacillus subtilis YphP, a prokaryotic disulfide isomerase with a CXC catalytic motif
    Urszula Derewenda
    Department of Molecular Physiology and Biological Physics and the ISFI PSI2 Center, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0736, USA
    Biochemistry 48:8664-71. 2009
    ..Functional assays confirmed that the protein displays a level of isomerase activity that might be biologically significant. We propose a mechanism by which the members of this family catalyze isomerization using the CXC catalytic site...
  20. ncbi request reprint The binding of the PDZ tandem of syntenin to target proteins
    Jolanta Grembecka
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0736, USA
    Biochemistry 45:3674-83. 2006
    ....
  21. ncbi request reprint The DC-module of doublecortin: dynamics, domain boundaries, and functional implications
    Tomasz Cierpicki
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908 0736, USA
    Proteins 64:874-82. 2006
    ....
  22. ncbi request reprint The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle
    Urszula Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Structure 12:1955-65. 2004
    ..The DH domain makes several specific interactions with RhoA residues not conserved among other Rho family members, suggesting the molecular basis for the observed specificity...
  23. pmc The crystal structure of the reduced, Zn2+-bound form of the B. subtilis Hsp33 chaperone and its implications for the activation mechanism
    Izabela Janda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Structure 12:1901-7. 2004
    ..coli protein, although there is no domain swapping. The Zn2+ binding domain is clearly resolved showing the details of the tetrahedral coordination of Zn2+ by four thiolates. We propose a structure-based activation pathway for Hsp33...
  24. ncbi request reprint Preliminary crystallographic analysis of the complex of the human GTPase RhoA with the DH/PH tandem of PDZ-RhoGEF
    Arkadiusz Oleksy
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 60:740-2. 2004
    ..The nucleotide-free DH/PH-RhoA complex was purified by gel filtration and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 88.6, b = 119.0, c = 91.5 A, beta = 114.7 degrees...
  25. ncbi request reprint Protein crystallization by surface entropy reduction: optimization of the SER strategy
    David R Cooper
    Department of Molecular Physiology and Biological Physics and Integrated Center for Structure Function Innovation, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 63:636-45. 2007
    ..Taken together, these results suggest a powerful crystallization strategy that combines surface engineering with efficient screening using standard and alternate reservoir solutions...
  26. ncbi request reprint The structure of the N-terminal domain of the product of the lissencephaly gene Lis1 and its functional implications
    Myung Hee Kim
    Department of Molecular Physiology and Biological Physics and Cancer Center, University of Virginia, Charlottesville, VA 22908, USA
    Structure 12:987-98. 2004
    ..The structure explains the molecular basis of a low severity form of lissencephaly...
  27. ncbi request reprint The PDZ2 domain of syntenin at ultra-high resolution: bridging the gap between macromolecular and small molecule crystallography
    Beom Sik Kang
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    J Mol Biol 338:483-93. 2004
    ..Most hydrogen atoms are easily identifiable in the electron density and weak hydrogen bonds of the C-H...O type are clearly visible between the beta-strands. The study sets a new standard for high-resolution protein crystallography...
  28. pmc The structure and ligand binding properties of the B. subtilis YkoF gene product, a member of a novel family of thiamin/HMP-binding proteins
    Yancho Devedjiev
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908 0736, USA
    J Mol Biol 343:395-406. 2004
    ..The atomic model of the complex was refined using data to 2.3 A resolution and revealed a unique H-bonding pattern that constitutes the molecular basis of specificity for the HMP moiety of thiamin...
  29. pmc The structure of DinB from Geobacillus stearothermophilus: a representative of a unique four-helix-bundle superfamily
    David R Cooper
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:219-24. 2010
    ..The proteins that share this fold exhibit four different quaternary structures: monomeric and three different dimeric forms...
  30. ncbi request reprint The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly
    Urszula Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Structure 15:1467-81. 2007
    ....
  31. pmc The solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA
    Tomasz Cierpicki
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, 22908, USA
    Protein Sci 18:2067-79. 2009
    ..The complex is readily and rapidly dissociated in the presence of both GDP and GTP nucleotides, with no evidence of intermediate ternary complexes...
  32. ncbi request reprint Probing the supramodular architecture of a multidomain protein: the structure of syntenin in solution
    Tomasz Cierpicki
    Department of Molecular Physiology, and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Structure 13:319-27. 2005
    ....
  33. ncbi request reprint Purification and crystallization of the N-terminal domain from the human doublecortin-like kinase
    Myung Hee Kim
    Department of Molecular Physiology and Biological Physics and The Cancer Center, University of Virginia, PO Box 800736, Charlottesville, Virginia 22908 0736, USA
    Acta Crystallogr D Biol Crystallogr 59:502-5. 2003
    ..33 A, beta = 101.3 degrees. Crystals of SeMet-substituted N-DCLK (Leu120Met) were also obtained, but they exhibit the symmetry of space group P2(1), with unit-cell parameters a = 38.81, b = 29.43, c = 40.1 A, beta = 115.7 degrees...
  34. ncbi request reprint PDZ tandem of human syntenin: crystal structure and functional properties
    Beom Sik Kang
    Department of Molecular Physiology and Biological Physics and The Cancer Center, University of Virginia, Charlottesville, VA 22908, USA
    Structure 11:459-68. 2003
    ..The independent binding of merlin by PDZ1 and syndecan-4 by PDZ2 provides direct evidence for the coupling of syndecan-mediated signaling to actin regulation by merlin...
  35. pmc B. subtilis ykuD protein at 2.0 A resolution: insights into the structure and function of a novel, ubiquitous family of bacterial enzymes
    Jakub Bielnicki
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Proteins 62:144-51. 2006
    ..The stereochemistry of this active site shows similarities to peptidotransferases and sortases, and suggests that the enzymes of the ykuD family may play an important role in cell wall biology...
  36. ncbi request reprint The DCX-domain tandems of doublecortin and doublecortin-like kinase
    Myung Hee Kim
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Nat Struct Biol 10:324-33. 2003
    ..In functional assays, the N-terminal DCX domain of doublecortin binds only to assembled microtubules, whereas the C-terminal domain binds to both microtubules and unpolymerized tubulin...
  37. ncbi request reprint Molecular roots of degenerate specificity in syntenin's PDZ2 domain: reassessment of the PDZ recognition paradigm
    Beom Sik Kang
    Department of Molecular Physiology and Biological Physics, The Cancer Center, University of Virginia, Charlottesville, VA 22908, USA
    Structure 11:845-53. 2003
    ..These results, in conjunction with other emerging structural data on PDZ domains, call for a revision of their classification and of the existing model of their mechanism...
  38. ncbi request reprint The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague
    Urszula Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Structure 12:301-6. 2004
    ....
  39. ncbi request reprint Molecular basis of mitomycin C resistance in streptomyces: structure and function of the MRD protein
    T W Martin
    Department of Molecular Physiology and Biological Physics, University of Virginia, Health Sciences System, Charlottesville 22908, USA
    Structure 10:933-42. 2002
    ..The location of the binding site is identical to the ones in evolutionarily related enzymes, suggesting that the protein may have been recruited from a different metabolic pathway...
  40. pmc Toward rational protein crystallization: A Web server for the design of crystallizable protein variants
    Lukasz Goldschmidt
    Howard Hughes Medical Institute, University of California, Los Angeles DOE Institute of Genomics and Proteomics, Los Angeles, California 90095 1570, USA
    Protein Sci 16:1569-76. 2007
    ..In most cases, the structure yielding mutations were easily identified by the SERp server. The server can be accessed at http://www.doe-mbi.ucla.edu/Services/SER...
  41. ncbi request reprint Coupling PAF signaling to dynein regulation: structure of LIS1 in complex with PAF-acetylhydrolase
    Cataldo Tarricone
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    Neuron 44:809-21. 2004
    ..Our data suggest that the LIS1 molecule undergoes major conformational rearrangement when switching from a complex with the acetylhydrolase to the one with Ndel1...
  42. pmc The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase
    Myung Hee Kim
    Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305 600, Korea
    Proc Natl Acad Sci U S A 102:17606-11. 2005
    ..We present experimental evidence that AHL-lactonase is a metalloenzyme containing two zinc ions involved in catalysis, and we propose a catalytic mechanism for bacterial metallo-AHL-lactonases...
  43. ncbi request reprint Degenerate specificity of PDZ domains from RhoA-specific nucleotide exchange factors PDZRhoGEF and LARG
    Katarzyna Smietana
    Faculty of Biotechnology, Laboratory of Protein Engineering, University of Wrocław, Wrocław, Poland
    Acta Biochim Pol 55:269-80. 2008
    ....
  44. ncbi request reprint The dimerization mechanism of LIS1 and its implication for proteins containing the LisH motif
    Agnieszka Mateja
    Laboratory of Protein Engineering, Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
    J Mol Biol 357:621-31. 2006
    ..The observed lability of the coiled-coil fragment in LIS1 is most likely of functional importance...
  45. ncbi request reprint The molecular basis of RhoA specificity in the guanine nucleotide exchange factor PDZ-RhoGEF
    Arkadiusz Oleksy
    Institute of Biochemistry and Molecular Biology, University of Wroclaw, 50 137 Wroclaw, Poland
    J Biol Chem 281:32891-7. 2006
    ..The key selectivity determinants are dominated by polar contacts involving residues unique to RhoA. We find that selectivity for RhoA versus Cdc42 is defined by a small number of interactions...
  46. ncbi request reprint Bivalent peptides as models for multimeric targets of PDZ domains
    Marcin Paduch
    Faculty of Biotechnology, Department of Protein Engineering, University of Wrocław, Tamka 2, 54 137 Wrocław, Poland
    Chembiochem 8:443-52. 2007
    ..This is consistent with the notion that multimerization of both receptors and PDZ-containing proteins might constitute an important regulatory mechanism...

Research Grants19

  1. Preparing high resolution membrane protein crystals
    Zygmunt Derewenda; Fiscal Year: 2005
    ..abstract_text> ..
  2. Molecular Mechanisms of Neuronal Migrations
    Zygmunt S Derewenda; Fiscal Year: 2010
    ..A comparison of results based on the Xenopus assays with the pull-down assays using mouse brain extracts will provide us with a unique database for Nud-protein interactions and invaluable insight into their function. ..
  3. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
    ..Funds are requested to further test the general applicability and success rate of this method, and to apply the method to a subset of targets selected for the Structural Genomic Initiative. ..
  4. Molecular Mechanisms of Neuronal Migration
    Zygmunt Derewenda; Fiscal Year: 2005
    ..We will also attempt to crystallize complexes of Lisi and DCX with downstream signaling partners. ..
  5. MECHANISMS OF LIPID-MEDIATED SIGNAL TRANSDUCTION
    Zygmunt Derewenda; Fiscal Year: 2001
    ....
  6. Molecular Mechanisms of Neuronal Migrations
    Zygmunt Derewenda; Fiscal Year: 2009
    ..A comparison of results based on the Xenopus assays with the pull-down assays using mouse brain extracts will provide us with a unique database for Nud-protein interactions and invaluable insight into their function. ..