U Derewenda

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF
    Meiying Zheng
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, Virginia, 22908 0736, USA
    BMC Struct Biol 9:36. 2009
  2. ncbi request reprint The crystal structure of a major dust mite allergen Der p 2, and its biological implications
    U Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Mol Biol 318:189-97. 2002
  3. ncbi request reprint Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib
    P J Sheffield
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906 0011, USA
    Protein Eng 14:513-9. 2001
  4. ncbi request reprint Sequence polymorphisms and antibody binding to the group 2 dust mite allergens
    A M Smith
    Asthma and Allergic Diseases Center, University of Virginia, Charlottesville, VA 22908 1355, USA
    Int Arch Allergy Immunol 124:61-3. 2001
  5. ncbi request reprint Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme
    J Li
    Department of Molecular Physiology and Biological Physics, University of Virginia, P O Box 800736, Charlottesville, Virginia 22908 0736, USA
    Nat Struct Biol 7:555-9. 2000
  6. ncbi request reprint How RhoGDI binds Rho
    K Longenecker
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906, USA
    Acta Crystallogr D Biol Crystallogr 55:1503-15. 1999
  7. ncbi request reprint Homologs of the alpha- and beta-subunits of mammalian brain platelet-activating factor acetylhydrolase Ib in the Drosophila melanogaster genome
    P J Sheffield
    Department of Molecular Physiology and Biological Physics, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908, USA
    Proteins 39:1-8. 2000
  8. ncbi request reprint The molecular basis of antigenic cross-reactivity between the group 2 mite allergens
    A M Smith
    Asthma and Allergic Diseases Center, Department of Medicine, University of Virginia Health System, Charlottesville, VA 22908-1355, USA
    J Allergy Clin Immunol 107:977-84. 2001
  9. ncbi request reprint Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer
    Y S Ho
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville 22906 0011, USA
    Nature 385:89-93. 1997
  10. ncbi request reprint Cross-reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens
    G Gafvelin
    Department of Medicine, Division of Clinical Immunology and Allergy, Karolinska Hospital and Institutet, Stockholm, Sweden
    J Allergy Clin Immunol 107:511-8. 2001

Collaborators

Detail Information

Publications13

  1. pmc On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF
    Meiying Zheng
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, Virginia, 22908 0736, USA
    BMC Struct Biol 9:36. 2009
    ..These GEFs include p115, LARG and PDZRhoGEF (PRG)...
  2. ncbi request reprint The crystal structure of a major dust mite allergen Der p 2, and its biological implications
    U Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Mol Biol 318:189-97. 2002
    ..The crystal structure suggests that binding of non-polar molecules may be essential to the physiological function of the Der p 2 protein...
  3. ncbi request reprint Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib
    P J Sheffield
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906 0011, USA
    Protein Eng 14:513-9. 2001
    ..The alpha(1)/alpha(2) dimer has been crystallized and its structure was solved at 2.1 A resolution by molecular replacement. These results set the stage for a detailed characterization of the PAF-AH Ib complex...
  4. ncbi request reprint Sequence polymorphisms and antibody binding to the group 2 dust mite allergens
    A M Smith
    Asthma and Allergic Diseases Center, University of Virginia, Charlottesville, VA 22908 1355, USA
    Int Arch Allergy Immunol 124:61-3. 2001
    ..Isoforms within each genus have been identified which differ by 3-4 amino acids. The aim of this study was to investigate the importance of these substitutions to antibody binding...
  5. ncbi request reprint Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme
    J Li
    Department of Molecular Physiology and Biological Physics, University of Virginia, P O Box 800736, Charlottesville, Virginia 22908 0736, USA
    Nat Struct Biol 7:555-9. 2000
    ..The catalytic site, inferred from the crystal structure and verified by site directed mutagenesis, involves novel chemistry and includes Asp 204, Gln 278 and Thr 228, which synergistically activate a nucleophilic water molecule...
  6. ncbi request reprint How RhoGDI binds Rho
    K Longenecker
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906, USA
    Acta Crystallogr D Biol Crystallogr 55:1503-15. 1999
    ..The entrance of the hydrophobic pocket of RhoGDI is 25 A from the last residue in the RhoA model, with its C-terminus oriented to accommodate the geranylgeranyl group without conformational change in RhoA...
  7. ncbi request reprint Homologs of the alpha- and beta-subunits of mammalian brain platelet-activating factor acetylhydrolase Ib in the Drosophila melanogaster genome
    P J Sheffield
    Department of Molecular Physiology and Biological Physics, University of Virginia, Health Sciences Center, Charlottesville, Virginia 22908, USA
    Proteins 39:1-8. 2000
    ..Our study shows that the beta-subunit homolog is highly conserved from Drosophila to mammals and is able to interact with the mammalian alpha-subunits but is unable to interact with the Drosophila alpha-subunit. Proteins 2000;39:1-8...
  8. ncbi request reprint The molecular basis of antigenic cross-reactivity between the group 2 mite allergens
    A M Smith
    Asthma and Allergic Diseases Center, Department of Medicine, University of Virginia Health System, Charlottesville, VA 22908-1355, USA
    J Allergy Clin Immunol 107:977-84. 2001
    ....
  9. ncbi request reprint Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer
    Y S Ho
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville 22906 0011, USA
    Nature 385:89-93. 1997
    ..The active site is made up of a trypsin-like triad of Ser 47, His 195 and Asp 192. Thus, the intact PAF-AH(Ib) molecule is an unusual G-protein-like (alpha1/alpha2)beta trimer...
  10. ncbi request reprint Cross-reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens
    G Gafvelin
    Department of Medicine, Division of Clinical Immunology and Allergy, Karolinska Hospital and Institutet, Stockholm, Sweden
    J Allergy Clin Immunol 107:511-8. 2001
    ..CONCLUSION: Gly d 2 showed high sequence homology to Lep d 2. Cross-reactivity was observed between Gly d 2, Lep d 2, and Tyr p 2, but only limited cross-reactivity was demonstrated between these 3 allergens and Der p 2...
  11. ncbi request reprint Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A
    V Ducros
    Department of Chemistry, Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
    J Biol Chem 275:23020-6. 2000
    ....
  12. ncbi request reprint Crystal structure of RhoA-GDP and its functional implications
    Y Wei
    Nat Struct Biol 4:699-703. 1997
    ..A 2.1 A resolution crystal structure of the human RhoA-GDP complex shows unique stereochemistry in the switch I region, which results in a novel mode of Mg2+ binding...
  13. ncbi request reprint Crystal structure, at 2.6-A resolution, of the Streptomyces lividans xylanase A, a member of the F family of beta-1,4-D-glycanases
    U Derewenda
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 269:20811-4. 1994
    ..The crystal structure supports the earlier assignment of Glu-128 and Glu-236 as the catalytic amino acids (Moreau, A., Roberge, M., Manin, C., Shareck, F., Kluepfel, D., and Morosoli, R. (1994) Biochem. J., in press)...