Research Topics
| Vincent J DenefSummaryAffiliation: University of California Country: USA Publications
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Detail Information
Publications
Strain-resolved community proteomics reveals recombining genomes of acidophilic bacteriaIan Lo
University of California, Berkeley, California 94720, USA
Nature 446:537-41. 2007....
Implications of strain- and species-level sequence divergence for community and isolate shotgun proteomic analysisVincent J Denef
Department of Earth and Planetary Science, University of California, Berkeley, California 94720, USA
J Proteome Res 6:3152-61. 2007..4% reduction of the initial protein coverage per 1% amino acid divergence and total identification loss at 86% AAI. Consequently, shotgun proteomics is capable of cross-strain identifications but avoids most cross-species false positives...
Proteomics-inferred genome typing (PIGT) demonstrates inter-population recombination as a strategy for environmental adaptationVincent J Denef
University of California, Berkeley, CA 94720, USA
Environ Microbiol 11:313-25. 2009..Our findings demonstrate the power of PIGT and reveal that recombination is a mechanism for fine-scale adaptation in this system...
Ecological distribution and population physiology defined by proteomics in a natural microbial communityRyan S Mueller
Earth and Planetary Science Department, University of California, Berkeley, CA 94720, USA
Mol Syst Biol 6:374. 2010..This research establishes an effective strategy to investigate ecological relationships between microbial physiology and the environment for whole communities in situ...
Proteogenomic basis for ecological divergence of closely related bacteria in natural acidophilic microbial communitiesVincent J Denef
University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 107:2383-90. 2010..In summary, the results show how subtle genetic variations can lead to distinct ecological strategies...
Quantitative proteomic analyses of the response of acidophilic microbial communities to different pH conditionsChristopher P Belnap
Microbiology Graduate Group, Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA
ISME J 5:1152-61. 2011....
In situ evolutionary rate measurements show ecological success of recently emerged bacterial hybridsVincent J Denef
Department of Earth and Planetary Science, University of California, Berkeley, CA 94720, USA
Science 336:462-6. 2012..The ecological success of hybrid bacterial types highlights the role of evolutionary processes in rapid adaptation within natural microbial communities...
AMD biofilms: using model communities to study microbial evolution and ecological complexity in natureVincent J Denef
Department of Earth and Planetary Science, University of California, Berkeley, CA, USA
ISME J 4:599-610. 2010..We discuss the value and limitations of tractable model microbial communities in developing molecular methods for microbial ecology and in uncovering principles that may explain behavior in more complex systems...
The dynamic genetic repertoire of microbial communitiesPaul Wilmes
Department of Earth and Planetary Science, University of California at Berkeley, Berkeley, CA 94720, USA
FEMS Microbiol Rev 33:109-32. 2009..Finally, we highlight the importance of virus-host dynamics in generating and shaping within-population diversity...
Population genomic analysis of strain variation in Leptospirillum group II bacteria involved in acid mine drainage formationSheri L Simmons
Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, California, United States of America
PLoS Biol 6:e177. 2008..Thus, the most likely explanation for the observed patterns of polymorphism is divergence of ancestral strains due to geographic isolation, followed by mixing and subsequent recombination...
Persisting viral sequences shape microbial CRISPR-based immunityAriel D Weinberger
Biophysics Graduate Group, University of California, Berkeley, California, United States of America
PLoS Comput Biol 8:e1002475. 2012..By maintaining old immunities, selection may be tuning CRISPR-mediated immunity against viruses reemerging from lysogeny or migration...
Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatilityPatrick S G Chain
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
Proc Natl Acad Sci U S A 103:15280-7. 2006....
Validation of a more sensitive method for using spotted oligonucleotide DNA microarrays for functional genomics studies on bacterial communitiesVincent J Denef
Laboratory of Microbial Ecology and Technology, Ghent, Belgium
Environ Microbiol 5:933-43. 2003..This increase in sensitivity is important if oligonucleotide microarrays are to be used for simultaneous monitoring of a range of functions performed by different microorganisms in the environment...
