H F DeLuca

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi Therapeutic potential of the 2-alkyl and 2-alkylidene-19-nor-(20S)-modified analogs of 1alpha,25-dihydroxyvitamin D3
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Steroid Biochem Mol Biol 89:67-73. 2004
  2. ncbi Vitamin D and the parenteral nutrition patient
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Gastroenterology 137:S79-91. 2009
  3. ncbi The vitamin D analogue 2MD increases bone turnover but not BMD in postmenopausal women with osteopenia: results of a 1-year phase 2 double-blind, placebo-controlled, randomized clinical trial
    Hector F DeLuca
    Deltanoid Pharmaceuticals and the Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
    J Bone Miner Res 26:538-45. 2011
  4. ncbi 2MD, a new anabolic agent for osteoporosis treatment
    L A Plum
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI, 53706 1544, USA
    Osteoporos Int 17:704-15. 2006
  5. ncbi Vitamin D: its role and uses in immunology
    H F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    FASEB J 15:2579-85. 2001
  6. ncbi Evolution of our understanding of vitamin D
    Hector F DeLuca
    The Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Nutr Rev 66:S73-87. 2008
  7. ncbi Vitamin D deficiency diminishes the severity and delays onset of experimental autoimmune encephalomyelitis
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Arch Biochem Biophys 513:140-3. 2011
  8. ncbi Overview of general physiologic features and functions of vitamin D
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Am J Clin Nutr 80:1689S-96S. 2004
  9. ncbi Calcitonin is a major regulator for the expression of renal 25-hydroxyvitamin D3-1alpha-hydroxylase gene in normocalcemic rats
    T Shinki
    Department of Biochemistry, School of Dentistry, Showa University, Hatanodai, Shinagawa ku, Tokyo 142 8555, USA
    Proc Natl Acad Sci U S A 96:8253-8. 1999
  10. ncbi Cloning and expression of the chicken 25-hydroxyvitamin D3 24-hydroxylase cDNA
    F Jehan
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706, USA
    Biochim Biophys Acta 1395:259-65. 1998

Collaborators

Detail Information

Publications92

  1. ncbi Therapeutic potential of the 2-alkyl and 2-alkylidene-19-nor-(20S)-modified analogs of 1alpha,25-dihydroxyvitamin D3
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Steroid Biochem Mol Biol 89:67-73. 2004
    ..These abbreviated side chain analogs will be developed for diseases where a rise in serum calcium is not desired, as for example, cancer, renal osteodystrophy, psoriasis and autoimmune diseases...
  2. ncbi Vitamin D and the parenteral nutrition patient
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Gastroenterology 137:S79-91. 2009
    ..Thus, vitamin D is essential not only for the skeleton but also many other organ systems. Recommendations for 25-hydroxyvitamin D3 levels for PN patients are presented...
  3. ncbi The vitamin D analogue 2MD increases bone turnover but not BMD in postmenopausal women with osteopenia: results of a 1-year phase 2 double-blind, placebo-controlled, randomized clinical trial
    Hector F DeLuca
    Deltanoid Pharmaceuticals and the Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
    J Bone Miner Res 26:538-45. 2011
    ....
  4. ncbi 2MD, a new anabolic agent for osteoporosis treatment
    L A Plum
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI, 53706 1544, USA
    Osteoporos Int 17:704-15. 2006
    ..This analog has now been extensively tested in aged ovariectomized female rats maintained on a diet adequate in calcium and phosphorus...
  5. ncbi Vitamin D: its role and uses in immunology
    H F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    FASEB J 15:2579-85. 2001
    ..The results suggest an important role for vitamin D in autoimmune disorders and provide a fertile and interesting area of research that may yield important new therapies...
  6. ncbi Evolution of our understanding of vitamin D
    Hector F DeLuca
    The Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Nutr Rev 66:S73-87. 2008
    ..All of this nevertheless illustrates that much more remains to be discovered and applied to our armaments for preventing and treating disease...
  7. ncbi Vitamin D deficiency diminishes the severity and delays onset of experimental autoimmune encephalomyelitis
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Arch Biochem Biophys 513:140-3. 2011
    ..Thus, vitamin D deficiency interferes with the development of this autoimmune disease rather than increasing susceptibility...
  8. ncbi Overview of general physiologic features and functions of vitamin D
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Am J Clin Nutr 80:1689S-96S. 2004
    ..This overview provides a brief description of the physiologic, endocrinologic, and molecular biologic characteristics of vitamin D. It also provides information on new selective analogs of 1alpha,25-dihydroyvitamin D3 for therapy...
  9. ncbi Calcitonin is a major regulator for the expression of renal 25-hydroxyvitamin D3-1alpha-hydroxylase gene in normocalcemic rats
    T Shinki
    Department of Biochemistry, School of Dentistry, Showa University, Hatanodai, Shinagawa ku, Tokyo 142 8555, USA
    Proc Natl Acad Sci U S A 96:8253-8. 1999
    ..These results suggest that CT plays an important role in the maintenance of serum 1alpha,25(OH)2D3 under normocalcemic physiological conditions, at least in rats...
  10. ncbi Cloning and expression of the chicken 25-hydroxyvitamin D3 24-hydroxylase cDNA
    F Jehan
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706, USA
    Biochim Biophys Acta 1395:259-65. 1998
    ..The deduced amino acid sequences are also very well conserved and 325 of them are identical among the four known 25-hydroxyvitamin D3 24-hydroxylases. This cDNA expressed in E. coli produces 24-hydroxylase activity...
  11. ncbi Regulation of 25-hydroxyvitamin D3 1alpha-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3
    H L Brenza
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706, USA
    Arch Biochem Biophys 381:143-52. 2000
    ..These results suggest that the stimulatory action of PTH is directly on the 1alpha-hydroxylase gene, while the repressive action of 1,25-(OH)2D3 does not involve the parathyroid gland but is nevertheless indirect...
  12. ncbi Regulation of the procine 1,25-dihydroxyvitamin D3-24-hydroxylase (CYP24) by 1,25-dihydroxyvitamin D3 and parathyroid hormone in AOK-B50 cells
    C Zierold
    College of Agricultural and Life Sciences, Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Arch Biochem Biophys 381:323-7. 2000
    ..In view of the above findings we concluded that AOK-B50 cells are a suitable model for further studying the mechanism of action of PTH on 24-hydroxylase mRNA...
  13. ncbi Parathyroid hormone regulates 25-hydroxyvitamin D(3)-24-hydroxylase mRNA by altering its stability
    C Zierold
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 98:13572-6. 2001
    ..None was found, suggesting that the destabilization site is elsewhere in the coding region...
  14. ncbi Hypophosphatemia and the development of rickets in osteopetrotic (op/op) mice
    L C McCary
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, USA
    J Bone Miner Res 12:1944-51. 1997
    ..However, the ability of the op/op mouse to mineralize bone at low serum calcium and phosphorus levels remains unexplained...
  15. ncbi Cloning of the mouse 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP1alpha) gene
    C Kimmel-Jehan
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Biochim Biophys Acta 1475:109-13. 2000
    ..This molecular cloning makes possible the generation of transgenic mice in order to further investigate the role and importance of the 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP1alpha)...
  16. ncbi Metabolism of 1alpha,25-dihydroxyvitamin D(3) in vitamin D receptor-ablated mice in vivo
    B Endres
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 39:2123-9. 2000
    ..The presence of 25-hydroxyvitamin D(3)-24-hydroxylase mRNA in the kidneys of these vitamin D receptor null mutant mice was confirmed by ribonuclease protection assay...
  17. ncbi A characterization of vitamin D-independent intestinal calcium absorption in the osteopetrotic (op/op) mouse
    L C McCary
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, 53706, USA
    Arch Biochem Biophys 368:249-56. 1999
    ..Therefore, the mechanism of vitamin D-independent intestinal calcium absorption in the op/op mouse is distinct from vitamin D-dependent intestinal calcium absorption...
  18. ncbi 2-Ethyl and 2-ethylidene analogues of 1alpha,25-dihydroxy-19-norvitamin D(3): synthesis, conformational analysis, biological activities, and docking to the modeled rVDR ligand binding domain
    Rafal R Sicinski
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Med Chem 45:3366-80. 2002
    ..The three-dimensional structure model of the rat vitamin D receptor and the computational docking of four synthesized (20R)-19-norvitamin D(3) analogues into its binding pocket are also reported...
  19. ncbi New 1alpha,25-dihydroxy-19-norvitamin D(3) compounds constrained in a single A-ring conformation: synthesis of the analogues by ring-closing metathesis route and their biological evaluation
    Agnieszka Glebocka
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Med Chem 52:3496-504. 2009
    ..These results confirm that the beta-chair form of the vitamin D ring A is necessary for the binding to the receptor...
  20. ncbi Cloning and characterization of the mouse vitamin D receptor promoter
    F Jehan
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 94:10138-43. 1997
    ..Furthermore, in the NIH 3T3 cell line, a 3- to 5-fold increase in response to forskolin treatment (an activator of adenylate cyclase and in turn of protein kinase A pathway) was observed...
  21. ncbi cDNA cloning and characterization of a vitamin D3 hydroxylase-associated protein
    R A Ettinger
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 269:176-82. 1994
    ..VDHAP is a novel chick kidney-specific inner membrane protein of mitochondria, which associates with a fraction of the 1,25-dihydroxyvitamin D3-24R-hydroxylase and 25-hydroxyvitamin D3-1 alpha-hydroxylase...
  22. ncbi 26-Hydroxylation of 1alpha,25-dihydroxyvitamin D3 does not occur under physiological conditions
    B Endres
    Department of Biochemistry, University of Wisconsin-Madison, 53706, USA
    Arch Biochem Biophys 388:127-34. 2001
    ....
  23. ncbi Osteopetrotic (op/op) mice are unable to maintain serum calcium levels despite hyperabsorption of calcium
    L C McCary
    Department of Biochemistry, University of Wisconsin Madison, Wisconsin 53706, USA
    Endocrinology 137:1049-56. 1996
    ..In addition, op/op mice also possessed vitamin D-stimulated intestinal calcium absorption, but this process was muted by the overwhelming vitamin D-independent mechanism of dietary calcium absorption...
  24. ncbi New highly calcemic 1 alpha,25-dihydroxy-19-norvitamin D(3) compounds with modified side chain: 26,27-dihomo- and 26,27-dimethylene analogs in 20S-series
    Rafal R Sicinski
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
    Steroids 67:247-56. 2002
    ....
  25. ncbi Cloning and origin of the two forms of chicken vitamin D receptor
    Z Lu
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin Madison 53706, USA
    Arch Biochem Biophys 339:99-106. 1997
    ..The significant difference especially at the N-terminus demonstrates divergence of this receptor during evolution of these species...
  26. ncbi Analysis of basal regulatory elements in the 25-hydroxyvitamin D3 1alpha-hydroxylase gene promoter
    H L Brenza
    Department of Biochemistry, University of Wisconsin-Madison, 53706, USA
    Arch Biochem Biophys 388:121-6. 2001
    ..Deletion of the most proximal AP-1 site causes a 60% loss of enhancer activity. The data suggest the presence of the AC repeat prevents the full potential activation of the 1alpha-hydroxylase promoter by factors binding to AP-1 sites...
  27. ncbi Analysis of binding of the 1,25-dihydroxyvitamin D3 receptor to positive and negative vitamin D response elements
    H M Darwish
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Arch Biochem Biophys 334:223-34. 1996
    ..A nuclear factor has also been found that binds downstream from the GGTTCA element in the PTH promoter. The binding site of this factor overlaps the PTH-DRE nucleotide sequence...
  28. ncbi New 2-alkylidene 1alpha,25-dihydroxy-19-norvitamin D3 analogues of high intestinal activity: synthesis and biological evaluation of 2-(3'-alkoxypropylidene) and 2-(3'-hydroxypropylidene) derivatives
    Agnieszka Glebocka
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    J Med Chem 49:2909-20. 2006
    ..The in vivo tests have revealed that the calcemic activity of all analogues in the E-series (5a, 6a, 6b) is considerably higher than that of the native hormone...
  29. ncbi New analogs of 2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 with conformationally restricted side chains: evaluation of biological activity and structural determination of VDR-bound conformations
    Janeen L Vanhooke
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Arch Biochem Biophys 460:161-5. 2007
    ..The structural comparisons suggest that the position of C21 in the ligand binding site may be an important determinant of biological activity...
  30. ncbi 1,25-Dihydroxyvitamin D3 reversibly blocks the progression of relapsing encephalomyelitis, a model of multiple sclerosis
    M T Cantorna
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Proc Natl Acad Sci U S A 93:7861-4. 1996
    ..Thus, the block by 1,25-(OH)2D3 is reversible. A deficiency of vitamin D resulted in an increased susceptibility to EAE. Thus, 1,25-(OH)2D3 or its analogs are potentially important for treatment of MS...
  31. ncbi Molecular structure of the rat vitamin D receptor ligand binding domain complexed with 2-carbon-substituted vitamin D3 hormone analogues and a LXXLL-containing coactivator peptide
    Janeen L Vanhooke
    Department of Biochemistry, University of Wisconsin Madison, 53711, USA
    Biochemistry 43:4101-10. 2004
    ..Electrostatic and hydrophobic interactions between the peptide and the LBD stabilize the active receptor conformation. This stablization appears necessary for crystal growth...
  32. ncbi 2-Methylene analogs of 1alpha-hydroxy-19-norvitamin D3: synthesis, biological activities and docking to the ligand binding domain of the rat vitamin D receptor
    Pawel Grzywacz
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Steroid Biochem Mol Biol 89:13-7. 2004
    ..Unexpectedly, the 25-methyl compound 4 showed marked calcemic activity in both assays. Computational docking of the vitamin 3 into the binding pocket of the rat vitamin D receptor is also reported...
  33. ncbi Model of three-dimensional structure of VDR bound with Vitamin D3 analogs substituted at carbon-2
    Wanda Sicinska
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Steroid Biochem Mol Biol 89:107-10. 2004
    ..We speculate that two hydrophobic receptor patches, being in close contact with ligand methyl groups, might influence interaction with co-modulators involved in calcium homeostasis...
  34. ncbi Methyl substitution of the 25-hydroxy group on 2-methylene-19-nor-1alpha,25-dihydroxyvitamin D3 (2MD) reduces potency but allows bone selectivity
    Pawel Grzywacz
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Arch Biochem Biophys 460:274-84. 2007
    ..However, analog 4 retains bone selectivity making it interesting as a possible therapeutic for bone loss diseases...
  35. ncbi New derivative of 1alpha,25-dihydroxy-19-norvitamin D3 with 3'-alkoxypropylidene moiety at C-2: synthesis, biological activity and conformational analysis
    Agnieszka Glebocka
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Steroid Biochem Mol Biol 89:25-30. 2004
    ..In comparison with hormone 1, it also showed slightly lower cellular HL-60-differentiation activity. Preliminary in vivo tests indicated unusually high calcemic activity of 7...
  36. ncbi Design, synthesis, and biological evaluation of a 1alpha,25-dihydroxy-19-norvitamin D3 analogue with a frozen A-ring conformation
    Rafal R Sicinski
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Med Chem 50:6154-64. 2007
    ..Surprisingly, in vivo tests in mice showed that the analogue 4 significantly increases serum calcium at dose levels similar to 1alpha,25-(OH)2D3. These seemingly discordant results are discussed...
  37. ncbi Responsiveness of human retinoblastoma and neuroblastoma models to a non-calcemic 19-nor Vitamin D analog
    Daniel M Albert
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, F4 344 Clinical Science Center, 600 Highland Avenue, Madison, WI 53792 3284, USA
    J Steroid Biochem Mol Biol 97:165-72. 2005
    ..To investigate the effectiveness of 2-methylene-19-nor-(20S)-1alpha-hydroxybishomopregnacalciferol (2MbisP) in inhibiting the growth of retinoblastoma (RB) and neuroblastoma (NB)...
  38. ncbi The role of vitamin A in mammalian reproduction and embryonic development
    Margaret Clagett-Dame
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Annu Rev Nutr 22:347-81. 2002
    ....
  39. ncbi 13-Methyl-substituted des-C,D analogs of (20S)-1alpha,25-dihydroxy-2-methylene-19-norvitamin D3 (2MD): synthesis and biological evaluation
    Katarzyna Plonska-Ocypa
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Bioorg Med Chem 17:1747-63. 2009
    ..The in vivo tests showed that all synthesized vitamin D analogs were devoid of calcemic activity...
  40. ncbi A potent analog of 1alpha,25-dihydroxyvitamin D3 selectively induces bone formation
    Nirupama K Shevde
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 99:13487-91. 2002
    ..These results indicate that 2MD is a potent bone-selective analog of 1,25(OH)(2)D(3) potentially effective in treating bone loss diseases...
  41. ncbi Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3
    M Strom
    Department of Biochemistry, College of Agricultural and Life Sciences, Madison, WI
    Biochim Biophys Acta 1090:299-304. 1991
    ..The corresponding mRNA is markedly increased by 6 h after a single dose of 1,25-dihydroxyvitamin D-3. The mRNA is also increased by 24-homologated analogs of 1,25-dihydroxyvitamin D-3 that do not increase calcium transport...
  42. ncbi 1,25-Dihydroxyvitamin D3 prolongs graft survival without compromising host resistance to infection or bone mineral density
    M T Cantorna
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Transplantation 66:828-31. 1998
    ....
  43. ncbi Isolation and characterization of a novel cDNA from HL-60 cells treated with 1,25-dihydroxyvitamin D-3
    K S Chen
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706
    Biochim Biophys Acta 1219:26-32. 1994
    ..Cycloheximide itself increases VDUP1 mRNA levels. These results suggest that the degradation of VDUP1 mRNA is either translation-dependent or regulated by a labile protein...
  44. ncbi Parathyroid hormone activation of the 25-hydroxyvitamin D3-1alpha-hydroxylase gene promoter
    H L Brenza
    Department of Biochemistry, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 95:1387-91. 1998
    ..These results indicate that parathyroid hormone stimulates 25-hydroxyvitamin D3-1alpha-hydroxylase by acting on the promoter of the 1alpha-hydroxylase gene...
  45. ncbi 1,25-Dihydroxyvitamin D3 up-regulates the renal vitamin D receptor through indirect gene activation and receptor stabilization
    Kevin D Healy
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 433:466-73. 2005
    ..The 1,25-(OH)(2)D(3) requirement can also be shown in TCMK-1 cells in vitro, while the calcium requirement was not found...
  46. ncbi CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1alpha-hydroxylase promoter activity in the skin
    Janeen L Vanhooke
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 103:75-80. 2006
    ..These findings stand in contrast to previously published reports of 1,25(OH)(2)D(3) production in keratinocytes...
  47. ncbi 1,25-Dihydroxyvitamin D3 regulates genes responsible for detoxification in intestine
    Galina D Kutuzova
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Toxicol Appl Pharmacol 218:37-44. 2007
    ..There is also an increased expression of antioxidant genes. These results support the idea that vitamin D is a significant factor in detoxification and protection against environmental toxins...
  48. ncbi Calbindin D9k is not required for 1,25-dihydroxyvitamin D3-mediated Ca2+ absorption in small intestine
    Shirin Akhter
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Arch Biochem Biophys 460:227-32. 2007
    ..It is, therefore, clear that calbindin D9k is not required for vitamin D-induced intestinal calcium absorption...
  49. ncbi Removal of the 20-methyl group from 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D(3) (2MD) selectively eliminates bone calcium mobilization activity
    Rafal Barycki
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Bioorg Med Chem 17:7658-69. 2009
    ..Curiously, removal of both the C-13-methyl and 20-methyl restored slightly the bone calcium mobilizing activity. Thus, the 21-nor analog of 2MD may provide a potent analog with a greater margin of safety than 2MD...
  50. ncbi Isolation and characterization of unsaturated fatty acids as natural ligands for the retinoid-X receptor
    Jonathan T Goldstein
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 420:185-93. 2003
    ..Our studies reveal tissue dependent profiles of RXR agonists and support the idea of unsaturated fatty acids as physiological ligands of RXR...
  51. ncbi Selective analogs of 1alpha,25-dihydroxyvitamin D3 for the study of specific functions of Vitamin D
    Hector F DeLuca
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Steroid Biochem Mol Biol 103:263-8. 2007
    ..Regardless of their clinical utility, they represent valuable research tools that can be used to study the specific functions of the Vitamin D hormone in vivo...
  52. ncbi Lithocholic acid can carry out in vivo functions of vitamin D
    Jamie A Nehring
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:10006-9. 2007
    ..It is clear, therefore, that LCA can be absorbed into the circulation to bind to the VDR at extra-intestinal sites. These findings lend support for the idea that the VDR may have evolved from an original role in detoxification...
  53. ncbi Identification of a unique subset of 2-methylene-19-nor analogs of vitamin D with comedolytic activity in the rhino mouse
    Nirca J Nieves
    Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Invest Dermatol 130:2359-67. 2010
    ..Further, the activity of vitamin D analogs in causing epidermal hyperproliferation has been distinguished from that resulting in a reduction in utricle size...
  54. ncbi New 1alpha,25-dihydroxy-19-norvitamin D3 analogs with a frozen A-ring conformation
    Agnieszka Glebocka
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Steroid Biochem Mol Biol 121:46-50. 2010
    ..Contrary to its counterpart 5, the analog 4 retained some affinity to vitamin D receptor...
  55. ncbi NMR assignments of tryptophan residue in apo and holo LBD-rVDR
    Wanda Sicinska
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Proteins 61:461-7. 2005
    ..The results indicate that the interaction between the ligands and Trp282 is not responsible for differences in calcemic activity observed in vitamin D analogs...
  56. ncbi 2α-Methyl-19-nor-(20S)-1,25-dihydroxyvitamin D(3) protects the insulin 2 knockout non-obese diabetic mouse from developing type 1 diabetes without hypercalcaemia
    C M Kiekhaefer
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Clin Exp Immunol 166:325-32. 2011
    ..These results demonstrate that the Ins2(-/-) NOD model provides a rapid and effective method for studying T1D and for assessing efficacy of anti-diabetic agents...
  57. ncbi Cloning of the human 1 alpha,25-dihydroxyvitamin D-3 24-hydroxylase gene promoter and identification of two vitamin D-responsive elements
    K S Chen
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Biochim Biophys Acta 1263:1-9. 1995
    ..Further characterization of these VDREs in various constructs with either a native or TK promoter suggests that both VDREs are required for the optimal induction of 24-hydroxylase expression by 1,25-(OH)2D3...
  58. ncbi Vitamin D receptor null mutant mice fed high levels of calcium are fertile
    L E Johnson
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Nutr 131:1787-91. 2001
    ..It seems that the defect in reproduction reported previously for VDR null mutant mice is not the lack of a direct effect of 1,25-dihydroxycholecalciferol on reproductive function but is the result of hypocalcemia...
  59. ncbi Metabolism of a physiological amount of all-trans-retinol in the vitamin A-deficient rat
    E A Werner
    Department of Biochemistry, University of Wisconsin-Madison, 53706, USA
    Arch Biochem Biophys 393:262-70. 2001
    ..As atROL completely fulfills all of the physiological roles of vitamin A, and 9cRA is not detected in any of the tissues analyzed, these results suggest that 9cRA may have no physiological relevance in the rat...
  60. ncbi Assay variation confounds the diagnosis of hypovitaminosis D: a call for standardization
    N Binkley
    Department of Medicine, Osteoporosis Clinical Center and Research Program, University of Wisconsin Madison, 2870 University Avenue, Suite 100, Madison, WI 53705, USA
    J Clin Endocrinol Metab 89:3152-7. 2004
    ..If the medical community is to make progress in correcting widespread hypovitaminosis D, 25OHD measurement must be standardized...
  61. ncbi 2-methylene-19-nor-20(S)-1alpha-hydroxy-bishomopregnacalciferol [20(S)-2MbisP], an analog of vitamin D3 [1,25(OH)2D3], does not stimulate intestinal phosphate absorption at levels previously shown to suppress parathyroid hormone
    Katie B Williams
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, United States
    Steroids 73:1277-84. 2008
    ..Further, 19-nor-vitamin D2 under the same circumstances does stimulate phosphate absorption. Thus, 2MbisP has significant potential in the management of secondary hyperparathyroidism of renal failure...
  62. ncbi Synthesis and biological properties of 2-methylene-19-nor-25-dehydro-1alpha-hydroxyvitamin D(3)-26,23-lactones--weak agonists
    Grazia Chiellini
    Department of Biochemistry, College of Agriculture and Life Sciences, 433 Babcock Drive, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Bioorg Med Chem 16:8563-73. 2008
    ..Our biological data show that neither compound has antagonistic activity but acts as weak agonists in vitro and in vivo...
  63. ncbi The calcitonin/calcitonin gene related peptide-alpha gene is not required for 1alpha,25-dihydroxyvitamin D3-mediated suppression of experimental autoimmune encephalomyelitis
    Bryan R Becklund
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
    Arch Biochem Biophys 488:105-8. 2009
    ..Furthermore, treatment with 1,25(OH)(2)D(3) suppressed EAE in CT/CGRP knock-out mice equal to that in wild type mice. Therefore, we conclude that CT is not necessary for 1,25(OH)(2)D(3)-mediated suppression of EAE...
  64. ncbi Parathyroid hormone decreases renal vitamin D receptor expression in vivo
    Kevin D Healy
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:4724-8. 2005
    ..001). These results demonstrate that PTH is a potent down-regulator of VDR expression in vivo...
  65. ncbi Regulation of renal vitamin D receptor is an important determinant of 1alpha,25-dihydroxyvitamin D(3) levels in vivo
    Matthew J Beckman
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, 53706 1544, USA
    Arch Biochem Biophys 401:44-52. 2002
    ....
  66. ncbi Reproductive defects are corrected in vitamin d-deficient female rats fed a high calcium, phosphorus and lactose diet
    Laura E Johnson
    Department of Biochemistry, University of Wisconsin-Madison, Madison 53706, USA
    J Nutr 132:2270-3. 2002
    ..It appears likely that the diminished reproductive performance attributed to vitamin D deficiency is the result of hypocalcemia and/or hypophosphatemia caused by vitamin D deficiency...
  67. ncbi Synthesis and biological evaluation of 6-methyl analog of 1alpha,25-dihydroxyvitamin D3
    Katarzyna Sokolowska
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Steroid Biochem Mol Biol 121:29-33. 2010
    ....
  68. ncbi Characterization of intestinal phosphate absorption using a novel in vivo method
    Katie Beth Williams
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Am J Physiol Endocrinol Metab 292:E1917-21. 2007
    ..Finally, unlike findings resulting from in vitro or ex vivo experiments, no evidence in vivo was obtained that phosphate absorption requires sodium or is inhibited by potassium...
  69. ncbi Enhancement of 1,25-dihydroxyvitamin D3-mediated suppression of experimental autoimmune encephalomyelitis by calcitonin
    Bryan R Becklund
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706 1544
    Proc Natl Acad Sci U S A 106:5276-81. 2009
    ..Moreover, CT decreased the dose of 1,25(OH)(2)D(3) required for disease suppression. Our results suggest that CT may be a significant factor but cannot account entirely for 1,25(OH)(2)D(3)-mediated suppression of EAE...
  70. ncbi Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium
    Kevin D Healy
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:9733-7. 2003
    ..This in vivo study demonstrates that 1,25(OH)2D3 and calcium are both required for renal VDR mRNA expression above a basal level, furthering our understanding of the complex regulation of renal VDR by 1,25(OH)2D3 and calcium...
  71. ncbi Oral administration of 1,25-dihydroxyvitamin D3 completely protects NOD mice from insulin-dependent diabetes mellitus
    Julia B Zella
    Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 417:77-80. 2003
    ..Our results indicate that vitamin D status is a determining factor of disease susceptibility and oral administration of 1,25(OH)(2)D(3) prevents diabetes onset in NOD mice through 200 days of age...
  72. ncbi 1-desoxy analog of 2MD: synthesis and biological activity of (20S)-25-hydroxy-2-methylene-19-norvitamin D3
    Izabela Sibilska
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Steroid Biochem Mol Biol 121:51-5. 2010
    ..The results of the biological tests in vivo are also discussed...
  73. ncbi Gene expression profiles in rat intestine identify pathways for 1,25-dihydroxyvitamin D(3) stimulated calcium absorption and clarify its immunomodulatory properties
    Galina D Kutuzova
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544, USA
    Arch Biochem Biophys 432:152-66. 2004
    ..Several other genes related to the immune system and angiogenesis whose expression was changed in response to 1,25-(OH)(2)D(3) provided evidence for an immunomodulatory and anti-angiogenic role of 1,25-(OH)(2)D(3)...
  74. ncbi A single receptor identical with that from intestine/T47D cells mediates the action of 1,25-dihydroxyvitamin D-3 in HL-60 cells
    H Goto
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706
    Biochim Biophys Acta 1132:103-8. 1992
    ..The substitution of this particular base does not alter the amino acid sequence however. Thus, the same receptor likely operates in differentiation and calcium transport functions...
  75. ncbi Retinoic acid is detected at relatively high levels in the CNS of adult rats
    Elizabeth A Werner
    Department of Biochemistry, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA
    Am J Physiol Endocrinol Metab 282:E672-8. 2002
    ..Because RA is not transported preferentially to brain, this tissue likely synthesizes RA more efficiently than other target tissues...
  76. ncbi Hypophosphatemia is responsible for skeletal muscle weakness of vitamin D deficiency
    Laurie Schubert
    Department of Biochemistry, University of Wisconsin Madison, 53706 1544, United States
    Arch Biochem Biophys 500:157-61. 2010
    ..Further, hypocalcemia together with vitamin D deficiency did not produce muscle weakness. These results strongly suggest that muscle weakness noted in rachitic patients is the result of the hypophosphatemia of vitamin D deficiency...
  77. ncbi UV radiation suppresses experimental autoimmune encephalomyelitis independent of vitamin D production
    Bryan R Becklund
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 107:6418-23. 2010
    ..These results suggest that UVR is likely suppressing disease independent of vitamin D production, and that vitamin D supplementation alone may not replace the ability of sunlight to reduce MS susceptibility...
  78. ncbi TRPV6 is not required for 1alpha,25-dihydroxyvitamin D3-induced intestinal calcium absorption in vivo
    Galina D Kutuzova
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 105:19655-9. 2008
    ..These and previous results using calbindin D9k null mutant mice illustrate that molecular events in the intestinal calcium absorption process in response to the active form of vitamin D remain to be defined...
  79. ncbi The vitamin D receptor is necessary for 1alpha,25-dihydroxyvitamin D(3) to suppress experimental autoimmune encephalomyelitis in mice
    Terrence F Meehan
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 53706, USA
    Arch Biochem Biophys 408:200-4. 2002
    ..We conclude that 1alpha,25-dihydroxyvitamin D(3) functions to suppress EAE through the well-known VDR and not through an undiscovered receptor or through a "nongenomic" mechanism...
  80. ncbi Calbindin D(9k) knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level
    Galina D Kutuzova
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 103:12377-81. 2006
    ..Thus, calbindin D(9k) is not required for viability, reproduction, or calcium homeostasis...
  81. ncbi Nuclear receptor 4A2 and C/EBPbeta regulate the parathyroid hormone-mediated transcriptional regulation of the 25-hydroxyvitamin D3-1alpha-hydroxylase
    Claudia Zierold
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Arch Biochem Biophys 460:233-9. 2007
    ..In addition, C/EBPbeta over-expression decreases the endogenous levels of both NR4A2 and 1alpha-hydroxylase mRNA...
  82. ncbi Biologically active noncalcemic analogs of 1alpha,25-dihydroxyvitamin D with an abbreviated side chain containing no hydroxyl
    Lori A Plum
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 101:6900-4. 2004
    ....
  83. ncbi 2-Methylene-19-nor-1alpha-hydroxyvitamin D3 analogs inhibit adipocyte differentiation and PPARgamma2 gene transcription
    Brian Thomson
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 460:192-201. 2007
    ....
  84. ncbi 2-Methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 potently stimulates gene-specific DNA binding of the vitamin D receptor in osteoblasts
    Hironori Yamamoto
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 278:31756-65. 2003
    ....
  85. ncbi Regulation of 25-hydroxyvitamin D3-24-hydroxylase mRNA by 1,25-dihydroxyvitamin D3 and parathyroid hormone
    Claudia Zierold
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 50706-1544, USA
    J Cell Biochem 88:234-7. 2003
    ..2-fold in AOK-B50 cells by PTH. Untranslated regions of the 24-hydroxylase mRNA in reporter gene assays did not confer PTH responsiveness. Further analysis of the coding region of the rat 24-hydroxylase may reveal sites of action of PTH...
  86. ncbi Vitamin D and autoimmune diabetes
    Julia B Zella
    Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    J Cell Biochem 88:216-22. 2003
    ....
  87. ncbi Differential recruitment of coactivators to the vitamin D receptor transcriptional complex by 1alpha,25-dihydroxyvitamin D3 analogs
    Marie K Schwinn
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 465:443-51. 2007
    ....
  88. ncbi Is the vitamin d receptor found in muscle?
    Yongji Wang
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706 1544, USA
    Endocrinology 152:354-63. 2011
    ....
  89. ncbi The importance of stereochemistry on the actions of vitamin D
    G Chiellini
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Curr Top Med Chem 11:840-59. 2011
    ..Based on current information, suggestions for the structure-based design of therapeutically valuable vitamin D analogs will conclude the review...
  90. ncbi CD8(+) T cells are not necessary for 1 alpha,25-dihydroxyvitamin D(3) to suppress experimental autoimmune encephalomyelitis in mice
    Terrence F Meehan
    Department of Biochemistry, University of Wisconsin, College of Agricultural and Life Sciences, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 99:5557-60. 2002
    ..We conclude that CD8(+) T cells neither are needed nor do they play a role in the prevention of EAE by 1,25-(OH)(2)D(3)...
  91. ncbi A ligand for the aryl hydrocarbon receptor isolated from lung
    Jiasheng Song
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706, USA
    Proc Natl Acad Sci U S A 99:14694-9. 2002
    ....
  92. ncbi 19nor-1,25-dihydroxyvitamin D2 specifically induces CYP3A9 in rat intestine more strongly than 1,25-dihydroxyvitamin D3 in vivo and in vitro
    Claudia Zierold
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706, USA
    Mol Pharmacol 69:1740-7. 2006
    ....