Thomas J Deerinck

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Multicolor and electron microscopic imaging of connexin trafficking
    Guido Gaietta
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 296:503-7. 2002
  2. pmc Roles of KChIP1 in the regulation of GABA-mediated transmission and behavioral anxiety
    Kun Xia
    State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410078, China
    Mol Brain 3:23. 2010
  3. pmc PKA, PKC, and AKAP localization in and around the neuromuscular junction
    G A Perkins
    Department of Neurosciences and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093 0608, USA
    BMC Neurosci 2:17. 2001
  4. pmc The application of fluorescent quantum dots to confocal, multiphoton, and electron microscopic imaging
    Thomas J Deerinck
    National Center of Microscopy and Imaging Research NCMIR, Center for Research on Biological Systems, University of California, San Diego UCSD School of Medicine, 1000 BSB, 9500 Gilman Drive, MC0608, La Jolla, CA 92093 0608, USA
    Toxicol Pathol 36:112-6. 2008
  5. pmc Visualizing flock house virus infection in Drosophila cells with correlated fluorescence and electron microscopy
    Jason Lanman
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 161:439-46. 2008
  6. ncbi request reprint Markers for correlated light and electron microscopy
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research and Center for Research in Biological Systems, University of California, San Diego, La Jolla, California 92093, USA
    Methods Cell Biol 79:575-91. 2007
  7. ncbi request reprint Isoform-specific PKA dynamics revealed by dye-triggered aggregation and DAKAP1alpha-mediated localization in living cells
    Brent R Martin
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Chem Biol 14:1031-42. 2007
  8. ncbi request reprint Light and electron microscopic localization of multiple proteins using quantum dots
    Thomas J Deerinck
    Department of Neurosciences, The National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, USA
    Methods Mol Biol 374:43-53. 2007
  9. pmc The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 1070 Basic Science Building MC 0608, 9500 Gilman Drive, La Jolla, CA 92093 0608, USA
    J Struct Biol 161:359-71. 2008
  10. pmc Investigating synapse formation and function using human pluripotent stem cell-derived neurons
    Ji Eun Kim
    Neurobiology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 108:3005-10. 2011

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Multicolor and electron microscopic imaging of connexin trafficking
    Guido Gaietta
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 296:503-7. 2002
    ..Selective imaging by correlated optical and electron microscopy of protein molecules of known ages will clarify fundamental processes of protein trafficking in situ...
  2. pmc Roles of KChIP1 in the regulation of GABA-mediated transmission and behavioral anxiety
    Kun Xia
    State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410078, China
    Mol Brain 3:23. 2010
    ..Our study suggests that KChIP1 is a synaptic protein that regulates behavioral anxiety by modulating inhibitory synaptic transmission, and drugs that act on KChIP1 may help to treat patients with mood disorders including anxiety...
  3. pmc PKA, PKC, and AKAP localization in and around the neuromuscular junction
    G A Perkins
    Department of Neurosciences and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093 0608, USA
    BMC Neurosci 2:17. 2001
    ..We found previously that a new class of AKAPs, dual-specific AKAPs, denoted D-AKAP1 and D-AKAP2, bind to RIalpha in addition to the RII subunits...
  4. pmc The application of fluorescent quantum dots to confocal, multiphoton, and electron microscopic imaging
    Thomas J Deerinck
    National Center of Microscopy and Imaging Research NCMIR, Center for Research on Biological Systems, University of California, San Diego UCSD School of Medicine, 1000 BSB, 9500 Gilman Drive, MC0608, La Jolla, CA 92093 0608, USA
    Toxicol Pathol 36:112-6. 2008
    ..Considerations for their application to immunolabeling for correlated light and electron microscopic analysis are discussed...
  5. pmc Visualizing flock house virus infection in Drosophila cells with correlated fluorescence and electron microscopy
    Jason Lanman
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 161:439-46. 2008
    ..Two features of the mitochondrial modification are approximately 60 nm spherules that line the outer membrane and the large chamber created by the convolution induced in the entire organelle...
  6. ncbi request reprint Markers for correlated light and electron microscopy
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research and Center for Research in Biological Systems, University of California, San Diego, La Jolla, California 92093, USA
    Methods Cell Biol 79:575-91. 2007
  7. ncbi request reprint Isoform-specific PKA dynamics revealed by dye-triggered aggregation and DAKAP1alpha-mediated localization in living cells
    Brent R Martin
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Chem Biol 14:1031-42. 2007
    ..Overall, effective separation of type I PKA is substrate dependent, whereas type II PKA dissociation relies on autophosphorylation...
  8. ncbi request reprint Light and electron microscopic localization of multiple proteins using quantum dots
    Thomas J Deerinck
    Department of Neurosciences, The National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, USA
    Methods Mol Biol 374:43-53. 2007
    ..Here we demonstrate an approach using quantum dots for pre-embedding immunolabeling of multiple diverse proteins for both LM and EM that overcomes many of these problems...
  9. pmc The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 1070 Basic Science Building MC 0608, 9500 Gilman Drive, La Jolla, CA 92093 0608, USA
    J Struct Biol 161:359-71. 2008
    ....
  10. pmc Investigating synapse formation and function using human pluripotent stem cell-derived neurons
    Ji Eun Kim
    Neurobiology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 108:3005-10. 2011
    ..These findings establish human pluripotent stem cell-derived neurons as a viable model for the study of synaptic differentiation and function under normal and disorder-associated conditions...
  11. pmc Picking faces out of a crowd: genetic labels for identification of proteins in correlated light and electron microscopy imaging
    Mark H Ellisman
    National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093 0608, USA
    Methods Cell Biol 111:139-55. 2012
    ..Here we review and discuss the current generation of genetic labels for direct protein identification by CLEM, addressing their relative strengths and weaknesses and in what experiments they would be most useful...
  12. pmc Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
    Guido M Gaietta
    National Center for Microscopy and Imaging Research, Center for Research on Biological Structure, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:17777-82. 2006
    ..Our studies provide previously undescribed insights into Golgi disassociation and reassembly during mitosis and offer a powerful approach to follow recombinant protein distribution in 4D imaging and correlated high-resolution analysis...
  13. ncbi request reprint Development of a model for microphysiological simulations: small nodes of ranvier from peripheral nerves of mice reconstructed by electron tomography
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Department of Neurosciences and the Center for Research on Biological Systems, University of California, San Diego, CA, USA
    Neuroinformatics 3:133-62. 2005
    ....
  14. ncbi request reprint Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Commun Adhes 10:181-6. 2003
    ....
  15. pmc Fibrinogen-induced perivascular microglial clustering is required for the development of axonal damage in neuroinflammation
    Dimitrios Davalos
    Gladstone Institute of Neurological Disease, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Nat Commun 3:1227. 2012
    ..Thus, early and progressive perivascular microglial clustering triggered by fibrinogen leakage upon blood-brain barrier disruption contributes to axonal damage in neuroinflammatory disease...
  16. pmc Loss of acinar cell IKK╬▒ triggers spontaneous pancreatitis in mice
    Ning Li
    Laboratory of Gene Regulation and Signal Transduction, Departments of Pharmacology and Pathology, School of Medicine, UCSD, La Jolla, California 92093 0723, USA
    J Clin Invest 123:2231-43. 2013
    ....
  17. pmc A structural basis for the assembly and functions of a viral polymer that inactivates multiple tumor suppressors
    Horng D Ou
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 151:304-19. 2012
    ..E4-ORF3 assembly creates avidity-driven interactions with PML and an emergent MRN binding interface. This reveals an elegant structural solution whereby a small protein forms a multivalent matrix that traps disparate tumor suppressors...
  18. pmc STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane
    Shenyuan L Zhang
    Department of Physiology and Biophysics and Center for Immunology, University of California, Irvine, California 92697, USA
    Nature 437:902-5. 2005
    ..We propose that STIM1 functions as the missing link between Ca2+ store depletion and SOC influx, serving as a Ca2+ sensor that translocates upon store depletion to the plasma membrane to activate CRAC channels...
  19. pmc Structure-function studies of blood and air capillaries in chicken lung using 3D electron microscopy
    John B West
    Department of Medicine, and National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, CA 92093 0623, USA
    Respir Physiol Neurobiol 170:202-9. 2010
    ..These EM studies show the supporting structures in greater detail than has previously been possible, particularly in 3D, and they allow a more complete analysis of the mechanical forces affecting avian pulmonary capillaries...
  20. ncbi request reprint Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots
    Ben N G Giepmans
    Department of Neurosciences, National Center for Microscopy and Imaging Research, and Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, 92093 0608, USA
    Nat Methods 2:743-9. 2005
    ..We provide examples of double and triple immunolabeling using light, electron and correlated microscopy in rat cells and mouse tissue. We conclude that QDs aid precise high-throughput determination of protein distribution...
  21. pmc Spinophilin facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling at the axonal wrist
    Stephanie L Bielas
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 129:579-91. 2007
    ..These findings suggest that spatially restricted adaptor-specific MAP reactivation through dephosphorylation is important in organization of the neuronal cytoskeleton...
  22. pmc Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells
    Alla F Fomina
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Exp Cell Res 291:150-66. 2003
    ..The present study demonstrates the utility of FM1-43 as a marker of membrane trafficking in T cells and reveals possible mechanisms of its modulation during T cell activation...
  23. pmc Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei
    Paula A Bubulya
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    J Cell Biol 167:51-63. 2004
    ..This work demonstrates a previously unrecognized role of NAPs in splicing factor trafficking and nuclear speckle biogenesis...
  24. ncbi request reprint Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors
    William Ju
    Nancy Friend Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, 1201 Welch Road, Stanford University School of Medicine, Palo Alto, California 94304, USA
    Nat Neurosci 7:244-53. 2004
    ....