Research Topics
Genomes and Genes | Russell A DeBose-BoydSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
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Publications
Opening up new fronts in the fight against cholesterolRussell A DeBose-Boyd
is at the Department of Molecular Genetics and the Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, United States
elife 2:e00663. 2013....
Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductaseRussell A DeBose-Boyd
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
Cell Res 18:609-21. 2008..Thus, sterol-accelerated degradation of reductase represents an example of how a general cellular process (ER-associated degradation) is used to control an important metabolic pathway (cholesterol synthesis)...
A helping hand for cytochrome p450 enzymesRussell A DeBose-Boyd
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Cell Metab 5:81-3. 2007..This action indicates an important role for Dap1/PGRMC1 in P450-catalyzed reactions, some of which are involved in the metabolism of sterols and pharmaceutical compounds...- Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductaseNavdar Sever
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 279:43136-47. 2004..These results provide formal genetic proof for the essential role of Insig-1 in feedback control of lipid synthesis in cultured cells... - Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domainNavdar Sever
Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
Mol Cell 11:25-33. 2003..Insig-1 appears to play an essential role in the sterol-mediated trafficking of two proteins with sterol-sensing domains, HMG CoA reductase and SCAP... - Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8Youngah Jo
Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
Proc Natl Acad Sci U S A 108:20503-8. 2011..Variations in the concentrations of any one of these proteins may account for differences in cell- and/or tissue-specific regulation of reductase degradation... - Ubiquitination of 3-hydroxy-3-methylglutaryl-CoA reductase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligaseBao Liang Song
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
J Biol Chem 279:28798-806. 2004.... - Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78Joon No Lee
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Biol Chem 281:39308-15. 2006.... - Sterol-induced dislocation of 3-hydroxy-3-methylglutaryl coenzyme A reductase from endoplasmic reticulum membranes into the cytosol through a subcellular compartment resembling lipid dropletsIsamu Z Hartman
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 74390 9046, USA
J Biol Chem 285:19288-98. 2010..Genetic, biochemical, and localization studies suggest a model in which reductase is dislodged into the cytosol from an ER subdomain closely associated with lipid droplets... - Isolation of sterol-resistant Chinese hamster ovary cells with genetic deficiencies in both Insig-1 and Insig-2Peter C W Lee
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9046, USA
J Biol Chem 280:25242-9. 2005..These results demonstrate an absolute requirement for Insig proteins in the regulatory system that mediates lipid homeostasis in animal cells... - Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductaseBao Liang Song
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Mol Cell 19:829-40. 2005..The current results identify gp78 as the E3 that initiates sterol-accelerated degradation of reductase, and Insig-1 as a bridge between gp78/VCP and the reductase substrate... - Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterolBao Liang Song
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Cell Metab 1:179-89. 2005.... - Insig-mediated, sterol-accelerated degradation of the membrane domain of hamster 3-hydroxy-3-methylglutaryl-coenzyme A reductase in insect cellsAndrew D Nguyen
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 284:26778-88. 2009..These studies define Insigs as the minimal requirement for sterol-accelerated degradation of the membrane domain of reductase in Drosophila S2 cells... - Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienolsBao Liang Song
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 281:25054-61. 2006..Taken together, these results provide a biochemical mechanism for the hypocholesterolemic effects of vitamin E that have been observed in animals and humans... - Membrane-associated ubiquitin ligase complex containing gp78 mediates sterol-accelerated degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductaseYoungah Jo
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 286:15022-31. 2011..These studies mark the initial steps in the characterization of the mammalian gp78 ubiquitin ligase complex, the further elucidation of which may yield important insights into mechanisms underlying gp78-mediated ERAD... - Hypoxia stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase through accumulation of lanosterol and hypoxia-inducible factor-mediated induction of insigsAndrew D Nguyen
Departments of Molecular Genetics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
J Biol Chem 282:27436-46. 2007..These results define a novel oxygen-sensing mechanism mediated by the combined actions of methylated intermediates in cholesterol synthesis and the hypoxia-activated transcription factor HIF-1alpha... - Amplification of the gene for SCAP, coupled with Insig-1 deficiency, confers sterol resistance in mutant Chinese hamster ovary cellsPeter C W Lee
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
J Lipid Res 48:1944-54. 2007..These results highlight the importance of SCAP/Insig ratios in normal sterol-regulated processing of SREBPs in cultured cells... - Mutations within the membrane domain of HMG-CoA reductase confer resistance to sterol-accelerated degradationPeter C W Lee
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
J Lipid Res 48:318-27. 2007..Thus, three different point mutations in reductase, all of which localize to the membrane domain, disrupt Insig binding and abolish sterol-accelerated degradation of the enzyme... - Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranesYoungah Jo
Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046
Mol Biol Cell 24:169-83. 2013.... - Protein sensors for membrane sterolsJoseph L Goldstein
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Cell 124:35-46. 2006..Here, we discuss recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake... - Control of cholesterol synthesis through regulated ER-associated degradation of HMG CoA reductaseYoungah Jo
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA
Crit Rev Biochem Mol Biol 45:185-98. 2010.... - Lipid-regulated degradation of HMG-CoA reductase and Insig-1 through distinct mechanisms in insect cellsRebecca A Faulkner
Howard Hughes Medical Institute, Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
J Lipid Res 54:1011-22. 2013..Together, these results establish Drosophila S2 cells as a model system to elucidate mechanisms through which lipid constituents of cell membranes (i.e., sterols and fatty acids) modulate the ERAD of Insig-1 and reductase... - Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3-methylglutaryl-CoA reductase stimulated by sterols and geranylgeraniolNavdar Sever
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 278:52479-90. 2003.... - Knowing when to let go: endosomal release of LDL from the LDL-ReceptorRussell A DeBose-Boyd
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Mol Cell 16:160-2. 2004.... - Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductaseJian Cao
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
Cell Metab 6:115-28. 2007..In summary, our study identifies Ufd1 as a cofactor of gp78, reveals an unappreciated function of Ufd1 in the ubiquitination reaction during ERAD, and illustrates that Ufd1 plays a critical role in cholesterol metabolism...
Research Grants
- Dissecting ER-Associated Degradation of a Membrane Protein in Drosophila S2 CellsRUSSELL ALFRED DEBOSE BOYD; Fiscal Year: 2010....