Russell A DeBose-Boyd

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Opening up new fronts in the fight against cholesterol
    Russell A DeBose-Boyd
    is at the Department of Molecular Genetics and the Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, United States
    elife 2:e00663. 2013
  2. pmc Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase
    Russell A DeBose-Boyd
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Cell Res 18:609-21. 2008
  3. ncbi request reprint A helping hand for cytochrome p450 enzymes
    Russell A DeBose-Boyd
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell Metab 5:81-3. 2007
  4. ncbi request reprint Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 279:43136-47. 2004
  5. ncbi request reprint Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain
    Navdar Sever
    Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 11:25-33. 2003
  6. pmc Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8
    Youngah Jo
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 108:20503-8. 2011
  7. ncbi request reprint Ubiquitination of 3-hydroxy-3-methylglutaryl-CoA reductase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligase
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Biol Chem 279:28798-806. 2004
  8. ncbi request reprint Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78
    Joon No Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 281:39308-15. 2006
  9. pmc Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes
    Youngah Jo
    Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Mol Biol Cell 24:169-83. 2013
  10. pmc Sterol-induced dislocation of 3-hydroxy-3-methylglutaryl coenzyme A reductase from endoplasmic reticulum membranes into the cytosol through a subcellular compartment resembling lipid droplets
    Isamu Z Hartman
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 74390 9046, USA
    J Biol Chem 285:19288-98. 2010

Research Grants

Collaborators

Detail Information

Publications25

  1. pmc Opening up new fronts in the fight against cholesterol
    Russell A DeBose-Boyd
    is at the Department of Molecular Genetics and the Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, United States
    elife 2:e00663. 2013
    ....
  2. pmc Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase
    Russell A DeBose-Boyd
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Cell Res 18:609-21. 2008
    ..Thus, sterol-accelerated degradation of reductase represents an example of how a general cellular process (ER-associated degradation) is used to control an important metabolic pathway (cholesterol synthesis)...
  3. ncbi request reprint A helping hand for cytochrome p450 enzymes
    Russell A DeBose-Boyd
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell Metab 5:81-3. 2007
    ..This action indicates an important role for Dap1/PGRMC1 in P450-catalyzed reactions, some of which are involved in the metabolism of sterols and pharmaceutical compounds...
  4. ncbi request reprint Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 279:43136-47. 2004
    ..These results provide formal genetic proof for the essential role of Insig-1 in feedback control of lipid synthesis in cultured cells...
  5. ncbi request reprint Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain
    Navdar Sever
    Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 11:25-33. 2003
    ..Insig-1 appears to play an essential role in the sterol-mediated trafficking of two proteins with sterol-sensing domains, HMG CoA reductase and SCAP...
  6. pmc Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8
    Youngah Jo
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 108:20503-8. 2011
    ..Variations in the concentrations of any one of these proteins may account for differences in cell- and/or tissue-specific regulation of reductase degradation...
  7. ncbi request reprint Ubiquitination of 3-hydroxy-3-methylglutaryl-CoA reductase in permeabilized cells mediated by cytosolic E1 and a putative membrane-bound ubiquitin ligase
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Biol Chem 279:28798-806. 2004
    ....
  8. ncbi request reprint Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78
    Joon No Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 281:39308-15. 2006
    ....
  9. pmc Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes
    Youngah Jo
    Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Mol Biol Cell 24:169-83. 2013
    ....
  10. pmc Sterol-induced dislocation of 3-hydroxy-3-methylglutaryl coenzyme A reductase from endoplasmic reticulum membranes into the cytosol through a subcellular compartment resembling lipid droplets
    Isamu Z Hartman
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 74390 9046, USA
    J Biol Chem 285:19288-98. 2010
    ..Genetic, biochemical, and localization studies suggest a model in which reductase is dislodged into the cytosol from an ER subdomain closely associated with lipid droplets...
  11. ncbi request reprint Isolation of sterol-resistant Chinese hamster ovary cells with genetic deficiencies in both Insig-1 and Insig-2
    Peter C W Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 280:25242-9. 2005
    ..These results demonstrate an absolute requirement for Insig proteins in the regulatory system that mediates lipid homeostasis in animal cells...
  12. ncbi request reprint Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 19:829-40. 2005
    ..The current results identify gp78 as the E3 that initiates sterol-accelerated degradation of reductase, and Insig-1 as a bridge between gp78/VCP and the reductase substrate...
  13. ncbi request reprint Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Metab 1:179-89. 2005
    ....
  14. pmc Insig-mediated, sterol-accelerated degradation of the membrane domain of hamster 3-hydroxy-3-methylglutaryl-coenzyme A reductase in insect cells
    Andrew D Nguyen
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 284:26778-88. 2009
    ..These studies define Insigs as the minimal requirement for sterol-accelerated degradation of the membrane domain of reductase in Drosophila S2 cells...
  15. ncbi request reprint Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 281:25054-61. 2006
    ..Taken together, these results provide a biochemical mechanism for the hypocholesterolemic effects of vitamin E that have been observed in animals and humans...
  16. pmc Membrane-associated ubiquitin ligase complex containing gp78 mediates sterol-accelerated degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    Youngah Jo
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 286:15022-31. 2011
    ..These studies mark the initial steps in the characterization of the mammalian gp78 ubiquitin ligase complex, the further elucidation of which may yield important insights into mechanisms underlying gp78-mediated ERAD...
  17. ncbi request reprint Hypoxia stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase through accumulation of lanosterol and hypoxia-inducible factor-mediated induction of insigs
    Andrew D Nguyen
    Departments of Molecular Genetics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Biol Chem 282:27436-46. 2007
    ..These results define a novel oxygen-sensing mechanism mediated by the combined actions of methylated intermediates in cholesterol synthesis and the hypoxia-activated transcription factor HIF-1alpha...
  18. ncbi request reprint Amplification of the gene for SCAP, coupled with Insig-1 deficiency, confers sterol resistance in mutant Chinese hamster ovary cells
    Peter C W Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Lipid Res 48:1944-54. 2007
    ..These results highlight the importance of SCAP/Insig ratios in normal sterol-regulated processing of SREBPs in cultured cells...
  19. ncbi request reprint Mutations within the membrane domain of HMG-CoA reductase confer resistance to sterol-accelerated degradation
    Peter C W Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Lipid Res 48:318-27. 2007
    ..Thus, three different point mutations in reductase, all of which localize to the membrane domain, disrupt Insig binding and abolish sterol-accelerated degradation of the enzyme...
  20. ncbi request reprint Protein sensors for membrane sterols
    Joseph L Goldstein
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell 124:35-46. 2006
    ..Here, we discuss recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake...
  21. pmc Control of cholesterol synthesis through regulated ER-associated degradation of HMG CoA reductase
    Youngah Jo
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Crit Rev Biochem Mol Biol 45:185-98. 2010
    ....
  22. pmc Lipid-regulated degradation of HMG-CoA reductase and Insig-1 through distinct mechanisms in insect cells
    Rebecca A Faulkner
    Howard Hughes Medical Institute, Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Lipid Res 54:1011-22. 2013
    ..Together, these results establish Drosophila S2 cells as a model system to elucidate mechanisms through which lipid constituents of cell membranes (i.e., sterols and fatty acids) modulate the ERAD of Insig-1 and reductase...
  23. ncbi request reprint Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3-methylglutaryl-CoA reductase stimulated by sterols and geranylgeraniol
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 278:52479-90. 2003
    ....
  24. ncbi request reprint Knowing when to let go: endosomal release of LDL from the LDL-Receptor
    Russell A DeBose-Boyd
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 16:160-2. 2004
    ....
  25. ncbi request reprint Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase
    Jian Cao
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Cell Metab 6:115-28. 2007
    ..In summary, our study identifies Ufd1 as a cofactor of gp78, reveals an unappreciated function of Ufd1 in the ubiquitination reaction during ERAD, and illustrates that Ufd1 plays a critical role in cholesterol metabolism...

Research Grants1