Valerie De Crecy Lagard

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. pmc The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA
    Basma El Yacoubi
    Department of Microbiology, University of Florida, Gainesville, FL 32611 0700, USA
    Nucleic Acids Res 37:2894-909. 2009
  2. pmc A genetic investigation of the KEOPS complex in halophilic Archaea
    Adit Naor
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    PLoS ONE 7:e43013. 2012
  3. pmc Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii
    Ian K Blaby
    Department of Microbiology and Cell Science, University of Florida, Gainesville, 32611 0700, USA
    Archaea 2010:426239. 2010
  4. pmc Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Department of Microbiology and Cell Science, University of Florida, P O Box 110700, Gainesville, Florida 32611 0700, United States
    ACS Chem Biol 7:1807-16. 2012
  5. pmc Comparative genomic analysis of the DUF71/COG2102 family predicts roles in diphthamide biosynthesis and B12 salvage
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
    Biol Direct 7:32. 2012
  6. doi request reprint Biosynthesis and function of posttranscriptional modifications of transfer RNAs
    Basma El Yacoubi
    Department of Microbiology, University of Florida, Gainesville, Florida 32611 0700, USA
    Annu Rev Genet 46:69-95. 2012
  7. pmc YeiR: a metal-binding GTPase from Escherichia coli involved in metal homeostasis
    Crysten E Blaby-Haas
    Department of Microbiology and Cell Science, University of Florida, PO Box 110700, Gainesville, FL 32611 0700, USA
    Metallomics 4:488-97. 2012
  8. pmc Predicting the pathway involved in post-translational modification of elongation factor P in a subset of bacterial species
    Marc Bailly
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
    Biol Direct 5:3. 2010
  9. pmc Synergistic use of plant-prokaryote comparative genomics for functional annotations
    Svetlana Gerdes
    Fellowship for Interpretation of Genomes, Burr Ridge, IL, USA
    BMC Genomics 12:S2. 2011
  10. pmc SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes
    Paul A Gulig
    Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA
    BMC Genomics 11:512. 2010

Research Grants

  1. Biosynthesis of hypermodified guanosines
    Valerie De Crecy Lagard; Fiscal Year: 2006
  2. Biosynthesis of hypermodified guanosines
    Valerie De Crecy Lagard; Fiscal Year: 2007
  3. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2007
  4. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2008
  5. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2009
  6. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2010
  7. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2009

Collaborators

Detail Information

Publications27

  1. pmc The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA
    Basma El Yacoubi
    Department of Microbiology, University of Florida, Gainesville, FL 32611 0700, USA
    Nucleic Acids Res 37:2894-909. 2009
    ..This work lays the foundation for elucidating the function of a protein family found in every sequenced genome to date and understanding the role of t(6)A in vivo...
  2. pmc A genetic investigation of the KEOPS complex in halophilic Archaea
    Adit Naor
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    PLoS ONE 7:e43013. 2012
    ..volcanii. Deletion of pcc1 led to pleiotropic phenotypes, including decreased growth rate, reduced levels of t(6)A modification, and elevated levels of intra-cellular glycation products...
  3. pmc Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii
    Ian K Blaby
    Department of Microbiology and Cell Science, University of Florida, Gainesville, 32611 0700, USA
    Archaea 2010:426239. 2010
    ..We conclude that these high-throughput methods are suitable for a rapid investigation of an H. volcanii mutant library and suggest that they should form the basis of a larger genome-wide experiment...
  4. pmc Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Department of Microbiology and Cell Science, University of Florida, P O Box 110700, Gainesville, Florida 32611 0700, United States
    ACS Chem Biol 7:1807-16. 2012
    ..The discovery of these missing genes solves a long-standing mystery and provides novel examples of convergent evolutions where proteins of dissimilar architectures perform the same biochemical function...
  5. pmc Comparative genomic analysis of the DUF71/COG2102 family predicts roles in diphthamide biosynthesis and B12 salvage
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
    Biol Direct 7:32. 2012
    ..Here, we illustrate this shift with the analysis of the DUF71/COG2102 family, a subgroup of the PP-loop ATPase family...
  6. doi request reprint Biosynthesis and function of posttranscriptional modifications of transfer RNAs
    Basma El Yacoubi
    Department of Microbiology, University of Florida, Gainesville, Florida 32611 0700, USA
    Annu Rev Genet 46:69-95. 2012
    ....
  7. pmc YeiR: a metal-binding GTPase from Escherichia coli involved in metal homeostasis
    Crysten E Blaby-Haas
    Department of Microbiology and Cell Science, University of Florida, PO Box 110700, Gainesville, FL 32611 0700, USA
    Metallomics 4:488-97. 2012
    ..These results support the predicted connection between the COG0523 P-loop GTPases and roles in metal homeostasis...
  8. pmc Predicting the pathway involved in post-translational modification of elongation factor P in a subset of bacterial species
    Marc Bailly
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
    Biol Direct 5:3. 2010
    ..The homologous lysine of EF-P is also modified to a spermidine derivative in Escherichia coli. However, the biosynthesis pathway of this modification in the bacterial EF-P is yet to be elucidated...
  9. pmc Synergistic use of plant-prokaryote comparative genomics for functional annotations
    Svetlana Gerdes
    Fellowship for Interpretation of Genomes, Burr Ridge, IL, USA
    BMC Genomics 12:S2. 2011
    ..This approach bridges the gap between automated homology-based annotations and the classical gene discovery efforts of experimentalists, and is more powerful than purely computational approaches to identifying gene-function associations...
  10. pmc SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes
    Paul A Gulig
    Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA
    BMC Genomics 11:512. 2010
    ..SOLiD sequencing of four V. vulnificus strains representing different clades (1 and 2) and biotypes (1 and 2) was used for comparative genomic analysis...
  11. pmc A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life
    Crysten E Haas
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
    BMC Genomics 10:470. 2009
    ..Even though the first COG0523-encoding gene, cobW, was identified almost 20 years ago, little is known concerning the function of other members belonging to this ubiquitous family...
  12. pmc RNomics and Modomics in the halophilic archaea Haloferax volcanii: identification of RNA modification genes
    Henri Grosjean
    Department of Microbiology, University of Florida, Gainsville, FL 32611, Florida, USA
    BMC Genomics 9:470. 2008
    ....
  13. ncbi request reprint Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Cell Science, University of Florida, FL, USA
    Mol Biol Evol 27:2062-77. 2010
    ..Finally, we proposed that the duplication of the trm5 gene in some Archaea led to a change in function from N1 methylation of guanosine to C7 methylation of 4-demethylwyosine (imG-14)...
  14. pmc Comparative genomics of bacterial and plant folate synthesis and salvage: predictions and validations
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
    BMC Genomics 8:245. 2007
    ..This study reports the results of the analysis and integrates them with new and existing experimental data...
  15. ncbi request reprint Comparative RNomics and modomics in Mollicutes: prediction of gene function and evolutionary implications
    VALERIE DE CRECY-LAGARD
    Department of Microbiology, University of Florida, Gainesville 32611 0700, USA
    IUBMB Life 59:634-58. 2007
    ....
  16. ncbi request reprint Finding novel metabolic genes through plant-prokaryote phylogenomics
    VALERIE DE CRECY-LAGARD
    Microbiology and Cell Science Department, University of Florida, Gainesville, FL 32611, USA
    Trends Microbiol 15:563-70. 2007
    ..This breaks with the past pattern of gene discovery, in which the information flow was most often unidirectional from prokaryotes to plants...
  17. ncbi request reprint Consistent alignment of metabolic pathways without abstraction
    Ferhat Ay
    Department of Computer Science and Engineering, University of Florida, Gainesville, FL 32611, USA
    Comput Syst Bioinformatics Conf 7:237-48. 2008
    ..Our experiments show that, our method finds biologically and statistically significant alignments in the order of seconds for pathways with approximately 100 entities...
  18. pmc 'Unknown' proteins and 'orphan' enzymes: the missing half of the engineering parts list--and how to find it
    Andrew D Hanson
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Biochem J 425:1-11. 2010
    ..Furthermore, it is cost-efficient and increasingly straightforward to deploy owing to a proliferation of databases and associated tools...
  19. ncbi request reprint A fast and accurate algorithm for comparative analysis of metabolic pathways
    Ferhat Ay
    Department of Computer Science and Engineering, University of Florida, Gainesville, FL 32611, USA
    J Bioinform Comput Biol 7:389-428. 2009
    ..Our experiments show that our method finds biologically and statistically significant alignments in the order of milliseconds...
  20. pmc Identification of genes encoding tRNA modification enzymes by comparative genomics
    VALERIE DE CRECY-LAGARD
    Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
    Methods Enzymol 425:153-83. 2007
    ..Methods that link gene and function but do not rely on sequence homology will be detailed, with examples taken from the tRNA modification field...
  21. pmc 6-pyruvoyltetrahydropterin synthase paralogs replace the folate synthesis enzyme dihydroneopterin aldolase in diverse bacteria
    Anne Pribat
    Horticultural Sciences Department, University of Florida, Gainesville, Florida 32611, USA
    J Bacteriol 191:4158-65. 2009
    ....
  22. pmc Biosynthesis of 7-deazaguanosine-modified tRNA nucleosides: a new role for GTP cyclohydrolase I
    Gabriella Phillips
    Department of Microbiology, University of Florida, Gainesville, FL 32611 0700, USA
    J Bacteriol 190:7876-84. 2008
    ..These results link the production of a tRNA-modified base to primary metabolism and further clarify the biosynthetic pathway for these complex modified nucleosides...
  23. pmc Phylogenomic and functional analysis of pterin-4a-carbinolamine dehydratase family (COG2154) proteins in plants and microorganisms
    Valeria Naponelli
    Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 146:1515-27. 2008
    ..Besides this role, we propose that partnerless PCDs support the function of as yet unrecognized pterin-dependent enzymes...
  24. pmc Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA
    Gabriela Phillips
    Department of Microbiology, University of Florida, Gainesville, Florida 32611 0700, USA
    J Biol Chem 285:12706-13. 2010
    ..This is the only example of the conversion of a nitrile to a formamidine known in biology and represents a new class of amidinotransferase chemistry...
  25. pmc FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosa
    Anne Pribat
    University of Florida, Horticultural Sciences Department, Gainesville, FL 32611, USA
    J Bacteriol 192:475-82. 2010
    ....
  26. pmc A Gateway platform for functional genomics in Haloferax volcanii: deletion of three tRNA modification genes
    Basma El Yacoubi
    Department of Microbiology and Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA
    Archaea 2:211-9. 2009
    ..Preliminary phenotypic analysis of the deletion mutants was conducted, and confirmed all three predictions...
  27. pmc A role for tetrahydrofolates in the metabolism of iron-sulfur clusters in all domains of life
    Jeffrey C Waller
    Horticultural Sciences, Food Science and Human Nutrition, Microbiology and Cell Science Departments, and Department of Biochemistry and Molecular Biology and National High Magnetic Field Laboratory, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 107:10412-7. 2010
    ..Thus COG0354 proteins have an ancient, conserved, folate-dependent function in the activity of certain Fe/S cluster enzymes...

Research Grants7

  1. Biosynthesis of hypermodified guanosines
    Valerie De Crecy Lagard; Fiscal Year: 2006
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. [unreadable] [unreadable] [unreadable]..
  2. Biosynthesis of hypermodified guanosines
    Valerie De Crecy Lagard; Fiscal Year: 2007
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. [unreadable] [unreadable] [unreadable]..
  3. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2007
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. ..
  4. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2008
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. ..
  5. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2009
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. ..
  6. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2010
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. ..
  7. Biosynthesis of hypermodified gaunosines.
    Valerie De Crecy Lagard; Fiscal Year: 2009
    ..Furthermore, one of the enzymes is a novel nitrile oxidoreductase that may have applications in industrial biocatalysis. ..