TRISHA DAVIS

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc The Yeast Resource Center Public Data Repository
    Michael Riffle
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 33:D378-82. 2005
  2. pmc Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B
    Jerry F Tien
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 189:713-23. 2010
  3. pmc Identification of Saccharomyces cerevisiae spindle pole body remodeling factors
    Kristen B Greenland
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 5:e15426. 2010
  4. pmc The Yeast Resource Center Public Image Repository: A large database of fluorescence microscopy images
    Michael Riffle
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    BMC Bioinformatics 11:263. 2010
  5. pmc Rings, bracelets, sleeves, and chevrons: new structures of kinetochore proteins
    Trisha N Davis
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Trends Cell Biol 17:377-82. 2007
  6. ncbi request reprint Protein localization in proteomics
    Trisha N Davis
    Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
    Curr Opin Chem Biol 8:49-53. 2004
  7. pmc Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase
    Michelle M Shimogawa
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Curr Biol 16:1489-501. 2006
  8. pmc Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces
    Tennessee J Yoder
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Biol Cell 16:141-52. 2005
  9. ncbi request reprint Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe
    Mark R Flory
    Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195, USA
    Cell Growth Differ 13:47-58. 2002
  10. pmc Reconstitution and characterization of budding yeast gamma-tubulin complex
    Dani B N Vinh
    Departments of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Biol Cell 13:1144-57. 2002

Research Grants

Collaborators

Detail Information

Publications32

  1. pmc The Yeast Resource Center Public Data Repository
    Michael Riffle
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 33:D378-82. 2005
    ..All of the data are accessible via searching by gene or protein name, and are available on the Web at http://www.yeastrc.org/pdr/...
  2. pmc Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B
    Jerry F Tien
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Cell Biol 189:713-23. 2010
    ..We propose that the action of the Dam1 complex as a processivity factor in kinetochore-microtubule attachment is regulated by conserved signals for error correction...
  3. pmc Identification of Saccharomyces cerevisiae spindle pole body remodeling factors
    Kristen B Greenland
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 5:e15426. 2010
    ..These findings implicate the nuclear pore, urmylation, and ubiquitination in SPB remodeling and represent novel functions for these genes...
  4. pmc The Yeast Resource Center Public Image Repository: A large database of fluorescence microscopy images
    Michael Riffle
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    BMC Bioinformatics 11:263. 2010
    ..Training and validating algorithms used in image analysis research typically rely on large sets of image data, and would benefit from a large, well-annotated and highly-available database of images and associated metadata...
  5. pmc Rings, bracelets, sleeves, and chevrons: new structures of kinetochore proteins
    Trisha N Davis
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Trends Cell Biol 17:377-82. 2007
    ..In this review, we compare and contrast the structure of these proteins and their interactions with microtubules to illustrate how they attach to and modulate the dynamics of microtubules...
  6. ncbi request reprint Protein localization in proteomics
    Trisha N Davis
    Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
    Curr Opin Chem Biol 8:49-53. 2004
    ..Alternatives to localizing tagged proteins are to use antibodies or aptamers to detect the untagged protein...
  7. pmc Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase
    Michelle M Shimogawa
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Curr Biol 16:1489-501. 2006
    ..Here we describe a phosphorylation event that promotes the coupling of kinetochores to microtubule plus ends...
  8. pmc Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces
    Tennessee J Yoder
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Biol Cell 16:141-52. 2005
    ..Our results provide insights into the relative contributions of the kinetochore and cytoplasmic microtubules to the forces involved in formation of a bipolar spindle...
  9. ncbi request reprint Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe
    Mark R Flory
    Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195, USA
    Cell Growth Differ 13:47-58. 2002
    ....
  10. pmc Reconstitution and characterization of budding yeast gamma-tubulin complex
    Dani B N Vinh
    Departments of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Biol Cell 13:1144-57. 2002
    ..Finally, we report the identification of a large 22 S Tub4p complex in yeast extract that contains multimers of Spc97p similar to gamma-tubulin ring complexes found in higher eukaryotic cells...
  11. pmc Phosphorylation of the chromosomal passenger protein Bir1 is required for localization of Ndc10 to the spindle during anaphase and full spindle elongation
    Per O Widlund
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Mol Biol Cell 17:1065-74. 2006
    ..We conclude that phosphorylation of the middle region of Bir1 is required to bring Ndc10 to the spindle at anaphase, which is required for full spindle elongation...
  12. pmc Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation
    Daniel R Gestaut
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 USA
    Nat Cell Biol 10:407-14. 2008
    ..Thus, even a small number of binding elements could provide a dynamic, phosphoregulated microtubule attachment and thereby facilitate accurate chromosome segregation...
  13. pmc Bir1 is required for the tension checkpoint
    Michelle M Shimogawa
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Mol Biol Cell 20:915-23. 2009
    ..Our results suggest that the establishment of tension removes Ipl1, Bir1, and Sli15, and their kinetochore detachment activity, from the vicinity of kinetochores and allows cells to proceed through the tension checkpoint...
  14. pmc Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis
    Andrew D Franck
    Department of Physiology, University of Washington, Seattle, WA 98195, USA
    Nat Cell Biol 9:832-7. 2007
    ..These effects are similar to the effects of tension on kinetochore-attached microtubule fibres in many cell types, suggesting that we have reconstituted a direct mechanism for microtubule-length control in mitosis...
  15. pmc The organization of the core proteins of the yeast spindle pole body
    Eric G D Muller
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Mol Biol Cell 16:3341-52. 2005
    ..One prediction of the model, the dimerization of the calmodulin-binding domains of Spc110, was confirmed by in vitro analysis...
  16. pmc The Saccharomyces cerevisiae spindle pole body is a dynamic structure
    Tennessee J Yoder
    Program in Molecular and Cellular Biology, University of Washington, Seattle, Washington 98195, USA
    Mol Biol Cell 14:3494-505. 2003
    ..Moreover, rather than being considered a conservative or dispersive process, the assembly of Spc110p into the SPB is more readily considered in terms of growth and exchange...
  17. pmc The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement
    Charles L Asbury
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:9873-8. 2006
    ..Our findings demonstrate how the Dam1 complex may contribute directly to MT-driven chromosome movement...
  18. pmc Reconstitution and functional analysis of kinetochore subcomplexes
    Daniel R Gestaut
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Methods Cell Biol 95:641-56. 2010
    ..Here we outline methods for rapid cloning of polycistronic vectors for expression of kinetochore subcomplexes, their purification, and techniques for functional analysis using total internal reflection fluorescence microscopy (TIRFM)...
  19. pmc The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion
    Andrew F Powers
    Department of Physiology and Biophysics, University of Washington, Seattle, 98195, USA
    Cell 136:865-75. 2009
    ..Our findings demonstrate how an ensemble of Ndc80 complexes may provide the combination of plasticity and strength that allows kinetochores to maintain load-bearing tip attachments during both microtubule assembly and disassembly...
  20. ncbi request reprint The centrosomal proteins pericentrin and kendrin are encoded by alternatively spliced products of one gene
    Mark R Flory
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA, 98195 USA
    Genomics 82:401-5. 2003
    ....
  21. pmc Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology
    Lars Malmström
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS Biol 5:e76. 2007
    ..The domain predictions and structural information are available in an online database at http://rd.plos.org/10.1371_journal.pbio.0050076_01...
  22. ncbi request reprint Localization of proteins that are coordinately expressed with Cln2 during the cell cycle
    Bryan A Sundin
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Yeast 21:793-800. 2004
    ..A complete list of localizations, along with images, can be found at our website (http://www.yeastrc.org/cln2/)...
  23. pmc Isotope signatures allow identification of chemically cross-linked peptides by mass spectrometry: a novel method to determine interresidue distances in protein structures through cross-linking
    Alex Zelter
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    J Proteome Res 9:3583-9. 2010
    ..The remaining 3 cross-links are likely due to protein aggregation. The accuracy and rapid throughput of our workflow will advance the use of protein cross-linking in structural biology...
  24. pmc A high-efficiency method to replace essential genes with mutant alleles in yeast
    Per O Widlund
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Yeast 22:769-74. 2005
    ..We have used this method to integrate a TS allele of SPC110 that could not be integrated by standard methods...
  25. pmc Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp
    Andrew D Franck
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Methods 51:242-50. 2010
    ....
  26. ncbi request reprint Assigning function to yeast proteins by integration of technologies
    Tony R Hazbun
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Mol Cell 12:1353-65. 2003
    ..This combination of technologies, coupled with annotation using GO, is a powerful approach to classifying genes...
  27. doi request reprint Insights into the kinetochore
    Charles L Asbury
    Structure 16:834-6. 2008
    ..Recently, Ciferri et al. (2008) published an atomic-level structure of the complex with implications for kinetochore architecture and for the generation and control of chromosome movements during mitosis...
  28. pmc The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation
    Justin M Kollman
    Department of Biochemistry and Biophysics and the Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 19:207-15. 2008
    ..We propose that further movement of the mobile arm is required to bring the gamma-tubulins together in microtubule-like interactions, and provide a template for microtubule growth...
  29. pmc Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore
    Vivien Measday
    Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4, Canada
    Genes Dev 16:101-13. 2002
    ..In contrast, Ctf3p and Ctf19p fail to bind properly to the centromere in a cse4-1 mutant strain. We conclude that the requirements for CENP-A loading onto centromere DNA differ in fission versus budding yeast...
  30. pmc In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae
    John Mc Intyre
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 26:3783-93. 2007
    ..Using FRET, we did not observe interactions between more than one cohesin complex in vivo...
  31. pmc Chl4p and iml3p are two new members of the budding yeast outer kinetochore
    Isabelle Pot
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada V5Z 4H4
    Mol Biol Cell 14:460-76. 2003
    ..These physical interaction dependencies provide insights into the molecular architecture and centromere DNA loading requirements of the outer kinetochore complex...
  32. ncbi request reprint Fluorescence resonance energy transfer using color variants of green fluorescent protein
    Dale W Hailey
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    Methods Enzymol 351:34-49. 2002

Research Grants51

  1. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    Trisha N Davis; Fiscal Year: 2010
    ..Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material. ..
  2. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2004
    ..The goals of this proposal are to characterize the assembly of the SPB in vivo, to reconstitute the nucleating activity of the SPB in vitro, and to study how the cell ensures assembly occurs properly. ..
  3. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2006
    ..The goals of this proposal are to characterize the assembly of the SPB in vivo, to reconstitute the nucleating activity of the SPB in vitro, and to study how the cell ensures assembly occurs properly. ..
  4. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 1999
    ..We will work towards developing assays for spindle pole body assembly by studying the incorporation of Spc110p and its interacting partners into the spindle pole body. ..
  5. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2002
    ..The goals of this proposal are to characterize the assembly of the SPB in vivo, to reconstitute the nucleating activity of the SPB in vitro, and to study how the cell ensures assembly occurs properly. ..
  6. MOLECULAR ANALYSIS OF CALMODULIN FUNCTION IN CELL GROWTH
    TRISHA DAVIS; Fiscal Year: 1992
    ..In the last two years of the proposal, the target proteins will be more thoroughly characterized and a mutational analysis of their function begun...
  7. MOLECULAR ANALYSIS OF CALMODULIN FUNCTION IN CELL GROWTH
    TRISHA DAVIS; Fiscal Year: 1990
    ..In the last two years of the proposal, the target proteins will be more thoroughly characterized and a mutational analysis of their function begun...
  8. MOLECULAR ANALYSIS OF CALMODULIN FUNCTION IN CELL GROWTH
    TRISHA DAVIS; Fiscal Year: 1993
    ..A fragment that hybridizes to HCM1 homolog. Finally, heat-sensitive calmodulin mutants of Schizosaccharomyces pombe will be isolated and characterized to discover if calmodulin performs a similar function in fission yeast...
  9. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2007
    ..Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material. ..
  10. Comprehensive Biology: Exploiting the Yeast Genome
    TRISHA DAVIS; Fiscal Year: 2006
    ..The multifaceted approach will develop the technologies needed to push biology forward, impacting not just the community of yeast biologists but also those working on all aspects of basic cell biology and human disease research. ..
  11. Comprehensive Biology: Exploiting the Yeast Genome
    TRISHA DAVIS; Fiscal Year: 2007
    ..The multifaceted approach will develop the technologies needed to push biology forward, impacting not just the community of yeast biologists but also those working on all aspects of basic cell biology and human disease research. ..
  12. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2009
    ..Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material. ..
  13. Comprehensive Biology: Exploiting the Yeast Genome
    TRISHA DAVIS; Fiscal Year: 2009
    ..The multifaceted approach will develop the technologies needed to push biology forward, impacting not just the community of yeast biologists but also those working on all aspects of basic cell biology and human disease research. ..
  14. Comprehensive Biology: Exploiting the Yeast Genome
    Trisha N Davis; Fiscal Year: 2010
    ..The multifaceted approach will develop the technologies needed to push biology forward, impacting not just the community of yeast biologists but also those working on all aspects of basic cell biology and human disease research. ..
  15. Comprehensive Biology: Exploiting the Yeast Genome
    TRISHA DAVIS; Fiscal Year: 2009
    ..The multifaceted approach will develop the technologies needed to push biology forward, impacting not just the community of yeast biologists but also those working on all aspects of basic cell biology and human disease research. ..
  16. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2009
    ..Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material. ..
  17. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2000
    ..We will work towards developing assays for spindle pole body assembly by studying the incorporation of Spc110p and its interacting partners into the spindle pole body. ..
  18. MOLECULAR ANALYSIS OF CALMODULIN FUNCTION IN CELL GROWTH
    TRISHA DAVIS; Fiscal Year: 1991
    ..In the last two years of the proposal, the target proteins will be more thoroughly characterized and a mutational analysis of their function begun...
  19. DISSECT ORGANIZATION OF SPINDLE POLE BODY USING FRET & DECONVOLUTION MICROSCOPY
    TRISHA DAVIS; Fiscal Year: 1999
    ..A linear increase in search time is expected for each additional processor used. The system is operational and is being optimized for speed. ..
  20. DISSECT ORGANIZATION OF SPINDLE POLE BODY USING FRET & DECONVOLUTION MICROSCOPY
    TRISHA DAVIS; Fiscal Year: 2000
    ..A linear increase in search time is expected for each additional processor used. The system is operational and is being optimized for speed. ..
  21. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2001
    ..We will work towards developing assays for spindle pole body assembly by studying the incorporation of Spc110p and its interacting partners into the spindle pole body. ..
  22. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2005
    ..The goals of this proposal are to characterize the assembly of the SPB in vivo, to reconstitute the nucleating activity of the SPB in vitro, and to study how the cell ensures assembly occurs properly. ..
  23. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2003
    ..The goals of this proposal are to characterize the assembly of the SPB in vivo, to reconstitute the nucleating activity of the SPB in vitro, and to study how the cell ensures assembly occurs properly. ..
  24. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2007
    ..Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material. ..
  25. MOLECULAR ANALYSIS OF THE SPINDLE POLE BODY
    TRISHA DAVIS; Fiscal Year: 2009
    ..Crucial to proper partitioning of the chromosomes during cell division is the establishment of the mitotic spindle, a microtubule- based molecular machine. The kinetochore is essential for this process. ..