Research Topics
Genomes and Genes | C William DavisSummaryAffiliation: University of North Carolina Country: USA Publications
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Detail Information
Publications
Regulation of mucin secretion from human bronchial epithelial cells grown in murine hosted xenograftsJason D Conway
Cystic Fibrosis Pulmonary Research and Treatment Center and Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
Am J Physiol Lung Cell Mol Physiol 284:L945-54. 2003..Hence, human airway goblet cells, like those of other species, appear to be regulated primarily via phospholipase C pathways, activated particularly by apical membrane P2Y(2)-R agonists...- Regulated airway goblet cell mucin secretionC William Davis
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599 7248, USA
Annu Rev Physiol 70:487-512. 2008..In contrast, synaptotagmin 2, a fast Ca2+ sensor for the SNARE complex, is essential for regulated secretion. Interestingly, these early results suggest that there are two pathways for excocytic mucin release from goblet cells... 
Coupling of airway ciliary activity and mucin secretion to mechanical stresses by purinergic signalingC William Davis
Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599 7248, United States
Respir Physiol Neurobiol 163:208-13. 2008..Of these, ATP, adenosine, UTP, and UDP act as agonists at apical P2Y2 (ATP, UTP), P2Y6 (UDP), and A2B (adenosine) receptors on ciliated and/or goblet cells to regulate mucociliary clearance...- Munc13-2-/- baseline secretion defect reveals source of oligomeric mucins in mouse airwaysYunxiang Zhu
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599 7248, USA
J Physiol 586:1977-92. 2008.... - Barrier role of actin filaments in regulated mucin secretion from airway goblet cellsCamille Ehre
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
Am J Physiol Cell Physiol 288:C46-56. 2005..We conclude that actin filaments negatively regulate mucin secretion basally in airway goblet cells and are dynamically remodeled in agonist-stimulated cells to promote exocytosis... - Coupled nucleotide and mucin hypersecretion from goblet-cell metaplastic human airway epitheliumSeiko F Okada
Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
Am J Respir Cell Mol Biol 45:253-60. 2011.... - nPKCepsilon, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cellsCamille Ehre
CCystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
Am J Physiol Cell Physiol 293:C1445-54. 2007..The translocation of nPKCdelta observed in activated cells is likely not related to mucin secretion but to some other aspect of goblet cell biology... - Regulation of mucin secretion from in vitro cellular modelsC William Davis
Department of Cell and Molecular Physiology, and Cystic Fibrosis/Pulmonary Research and Treatment Center, 6009 Thurston-Bowles, University of North Carolina, Chapel Hill, NC 27599-7248, USA
Novartis Found Symp 248:113-25; discussion 125-31, 277-82. 2002.... - Mucociliary interactions and mucus dynamics in ciliated human bronchial epithelial cell culturesPatrick R Sears
Cystic Fibrosis Center, University of North Carolina, Chapel Hill, NC 27599, USA
Am J Physiol Lung Cell Mol Physiol 301:L181-6. 2011..The significance of these findings is that aberrant modulation of such an intrinsic property may explain the initiation of highly adherent mucus in cystic fibrosis lung disease... - Calcium signaling in human airway goblet cells following purinergic activationAndrea H Rossi
CF Pulmonary Research and Treatment Center, Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC 27599 7248, USA
Am J Physiol Lung Cell Mol Physiol 292:L92-8. 2007..1 +/- 7% of the ATPgammaS control peak), brief rise in Ca(i)(2+). This diminutive signal likely denotes a local Ca(2+) gradient, which may be associated with the mucin granule exocytotic process... - Regulation of airway goblet cell mucin secretion by tyrosine phosphorylation signaling pathwaysLubna H Abdullah
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Am J Physiol Lung Cell Mol Physiol 293:L591-9. 2007..Hence, PLC signaling may play a key role in regulated mucin secretion, whether the event is initiated by mediators interacting with GPCRs or RYKs... - Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cellsSilvia M Kreda
Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 7017 Thurston Bowles Building, Chapel Hill, NC 27599 7248, USA
J Physiol 584:245-59. 2007..Our data suggest that nucleotide release is a mechanism by which mucin-secreting goblet cells produce paracrine signals for mucin hydration within the ASL... - Mechanosensitivity of mouse tracheal ciliary beat frequency: roles for Ca2+, purinergic signaling, tonicity, and viscosityScot L Winters
Department of Medicine, Cystic Fibrosis Pulmonary Research and Tteatment Center, University of North Carolina, Chapel Hill, NC 27599 7248, USA
Am J Physiol Lung Cell Mol Physiol 292:L614-24. 2007.... - Mucin secretion and PKC isoforms in SPOC1 goblet cells: differential activation by purinergic agonist and PMALubna H Abdullah
Cystic Fibrosis/Pulmonary Treatment and Research Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599-7248, USA
Am J Physiol Lung Cell Mol Physiol 285:L149-60. 2003..Hence, P2Y2 agonists activate nPKCdelta in SPOC1 cells. PMA activates cPKCalpha and nPKCdelta at high affinity and stimulates a lower affinity PKC-independent pathway that leads to mucin secretion... 
Studying mucin secretion from human bronchial epithelial cell primary culturesLubna H Abdullah
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC, USA
Methods Mol Biol 842:259-77. 2012..These results indicate that the more nonspecific assays are suitable to assess overall secretory responses by goblet cells, but are likely unsuited for specific measurements of polymeric mucins, per se...- Ca2+ dependency of 'Ca2+-independent' exocytosis in SPOC1 airway goblet cellsAndrea H Rossi
6009 Thurston-Bowles, University of North Carolina, Chapel Hill, NC 27599-7248, USA
J Physiol 559:555-65. 2004..PMA-induced secretion in EGTA was insensitive to heparin. These results suggest that Ca2+ is released locally during PMA-induced exocytosis, by an IP3-independent mechanism... - Force generation and dynamics of individual cilia under external loadingDavid B Hill
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Biophys J 98:57-66. 2010..By applying a quasistatic force model, we deduce that axoneme stiffness is dominated by the rigidity of the microtubules, and that cilia can exert 62 +/- 18 pN of force at the tip via the generation of 5.6 +/- 1.6 pN/dynein head... - Human airway ciliary dynamicsPatrick R Sears
Cystic Fibrosis Center, University of North Carolina, 6026 Thurston Bowles Bldg, CB7248, Chapel Hill, NC 27599, USA
Am J Physiol Lung Cell Mol Physiol 304:L170-83. 2013..31(0.04), 0.25(0.06), 0.50(0.12), 0.50(0.10), μm, respectively. In contrast, the same measures for three different PCD waveforms had values far outside this range... - CFTR, mucins, and mucus obstruction in cystic fibrosisSilvia M Kreda
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27517 7248, USA
Cold Spring Harb Perspect Med 2:a009589. 2012..In any event, the path between CFTR dysfunction and mucus hyperproduction has proven tortuous, and its unraveling continues to offer its own twists and turns, along with fascinating glimpses into biology... - Mucins and their O-Glycans from human bronchial epithelial cell culturesJessica M Holmén
Department of Medical Biochemistry, Göteborgs Universitet, 405 30 Gothenburg, Sweden
Am J Physiol Lung Cell Mol Physiol 287:L824-34. 2004..We conclude that the differences observed in the mucins sampled directly from patients are most likely due to environmental factors relating to infection and/or inflammation... - Airway epithelium, inflammation, and mechanisms of disease: A tribute to Carol B. BasbaumKenneth B Adler
Am J Respir Cell Mol Biol 34:523-6. 2006
Research Grants
- INTRACELLULAR PATHWAYS MEDIATING AIRWAY MUCIN SECRETIONC Davis; Fiscal Year: 2003..Lastly, we will exploit the different binding kinetics of BAPTA and EGTRA to test the degree of independence between PKC and Ca2+ in the final steps of exocytosis. ..
- Regulation of Airway Goblet Cell Mucin SecretionC Davis; Fiscal Year: 2007..abstract_text> ..
- Molecular Pathways Regulating Airway Goblet Cell Mucin SecretionBurton F Dickey; Fiscal Year: 2010....