W Davidson

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. ncbi request reprint The structure of apolipoprotein A-II in discoidal high density lipoproteins
    R A Gangani D Silva
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
    J Biol Chem 282:9713-21. 2007
  2. pmc Apolipoprotein A-I structural organization in high-density lipoproteins isolated from human plasma
    Rong Huang
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio, USA
    Nat Struct Mol Biol 18:416-22. 2011
  3. pmc Proteomic analysis of defined HDL subpopulations reveals particle-specific protein clusters: relevance to antioxidative function
    W Sean Davidson
    Department of Pathobiology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Arterioscler Thromb Vasc Biol 29:870-6. 2009
  4. ncbi request reprint The structure of apolipoprotein A-I in high density lipoproteins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, OH 45237, USA
    J Biol Chem 282:22249-53. 2007
  5. ncbi request reprint The spatial organization of apolipoprotein A-I on the edge of discoidal high density lipoprotein particles: a mass specrometry study
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529
    J Biol Chem 278:27199-207. 2003
  6. ncbi request reprint The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
    J Lipid Res 47:440-9. 2006
  7. ncbi request reprint Apolipoprotein structural organization in high density lipoproteins: belts, bundles, hinges and hairpins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    Curr Opin Lipidol 16:295-300. 2005
  8. ncbi request reprint 5th Annual International Conference on HDL Cholesterol: metabolic pathways and drug developments
    W Sean Davidson
    Department of Pathology, University of Cincinnati Genome Research Institute, 2120 East Galbraith Road, Building A ML 0507, Cincinnati, OH 45237, USA
    Expert Opin Ther Targets 8:359-66. 2004
  9. ncbi request reprint Apolipoprotein A-I alpha -helices 7 and 8 modulate high density lipoprotein subclass distribution
    Erica J Reschly
    Department of Pathology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 277:9645-54. 2002
  10. ncbi request reprint Specific sequences in the N and C termini of apolipoprotein A-IV modulate its conformation and lipid association
    Kevin Pearson
    Department of Pathology and Laboratory Medicine, The University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    J Biol Chem 280:38576-82. 2005

Collaborators

Detail Information

Publications38

  1. ncbi request reprint The structure of apolipoprotein A-II in discoidal high density lipoproteins
    R A Gangani D Silva
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
    J Biol Chem 282:9713-21. 2007
    ..The data clearly refute a parallel model but support two antiparallel models, especially a "double hairpin" form. These models form the basis for understanding apoA-II structure in more complex HDL particles...
  2. pmc Apolipoprotein A-I structural organization in high-density lipoproteins isolated from human plasma
    Rong Huang
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio, USA
    Nat Struct Mol Biol 18:416-22. 2011
    ..This understanding offers insights into how apoA-I structure modulates HDL function and its interactions with other apolipoproteins...
  3. pmc Proteomic analysis of defined HDL subpopulations reveals particle-specific protein clusters: relevance to antioxidative function
    W Sean Davidson
    Department of Pathobiology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Arterioscler Thromb Vasc Biol 29:870-6. 2009
    ..We hypothesized that distinct clusters of protein components may distinguish between physicochemically-defined subpopulations of HDL particles, and that such clusters may exert specific biological function(s)...
  4. ncbi request reprint The structure of apolipoprotein A-I in high density lipoproteins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, OH 45237, USA
    J Biol Chem 282:22249-53. 2007
  5. ncbi request reprint The spatial organization of apolipoprotein A-I on the edge of discoidal high density lipoprotein particles: a mass specrometry study
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529
    J Biol Chem 278:27199-207. 2003
    ....
  6. ncbi request reprint The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
    J Lipid Res 47:440-9. 2006
    ..5 h, consuming only 120 microg of apolipoprotein in total. The benefits and potential drawbacks of the assay are discussed...
  7. ncbi request reprint Apolipoprotein structural organization in high density lipoproteins: belts, bundles, hinges and hairpins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    Curr Opin Lipidol 16:295-300. 2005
    ..To summarize recent advances towards an understanding of the three-dimensional structures of the apolipoprotein components of HDL with a specific focus on high resolution models of apolipoprotein A-I...
  8. ncbi request reprint 5th Annual International Conference on HDL Cholesterol: metabolic pathways and drug developments
    W Sean Davidson
    Department of Pathology, University of Cincinnati Genome Research Institute, 2120 East Galbraith Road, Building A ML 0507, Cincinnati, OH 45237, USA
    Expert Opin Ther Targets 8:359-66. 2004
    ..A detailed understanding of the molecular basis for the protective effect of HDL will hopefully lead to the development of new therapeutics that exploit this pathway...
  9. ncbi request reprint Apolipoprotein A-I alpha -helices 7 and 8 modulate high density lipoprotein subclass distribution
    Erica J Reschly
    Department of Pathology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 277:9645-54. 2002
    ..Human apoA-I self-associates more and activates human lecithin-cholesterol acyltransferase better than mouse apoA-I. These differential characteristics of human and mouse apoA-I are not dependent on helices 7/8...
  10. ncbi request reprint Specific sequences in the N and C termini of apolipoprotein A-IV modulate its conformation and lipid association
    Kevin Pearson
    Department of Pathology and Laboratory Medicine, The University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    J Biol Chem 280:38576-82. 2005
    ..We propose a structural model in which these sequences can modulate the conformation and lipid affinity of apoA-IV...
  11. pmc Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes
    R A Gangani D Silva
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237, USA
    Proc Natl Acad Sci U S A 105:12176-81. 2008
    ..We propose the first experiment-based molecular model of apoA-I in spherical HDL particles. This model provides a new foundation for understanding how apoA-I structure modulates HDL function and metabolism...
  12. pmc A three-dimensional homology model of lipid-free apolipoprotein A-IV using cross-linking and mass spectrometry
    Matthew R Tubb
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
    J Biol Chem 283:17314-23. 2008
    ..This first structural model of lipid-free apoA-IV should prove useful for designing studies aimed at understanding how apoA-IV interacts with lipids and possibly with unknown protein partners...
  13. ncbi request reprint Modulation of apolipoprotein A-IV lipid binding by an interaction between the N and C termini
    Matthew R Tubb
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
    J Biol Chem 282:28385-94. 2007
    ..This interaction could represent a unique "switch" mechanism by which apoA-IV changes lipid avidity in vivo...
  14. ncbi request reprint Expression of biologically active rat apolipoprotein AIV in Escherichia coli
    Min Liu
    Department of Pathology, University of Cincinnati School of Medicine, Cincinnati, OH 45267 0529, USA
    Physiol Behav 78:149-55. 2003
    ....
  15. pmc Proteomic characterization of human plasma high density lipoprotein fractionated by gel filtration chromatography
    Scott M Gordon
    Center for Lipid and Arteriosclerosis Science, University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    J Proteome Res 9:5239-49. 2010
    ..The observed heterogeneity across subfractions suggests the presence of HDL particle subpopulations each with distinct protein components that may prove to impart distinct physiological functions...
  16. ncbi request reprint Identification and structural ramifications of a hinge domain in apolipoprotein A-I discoidal high-density lipoproteins of different size
    J Nicholas Maiorano
    Department of Pathology and Laboratory Medicine, University of Cincinnati, 2120 East Galbraith Road, Cincinnati, Ohio 45237 0507, USA
    Biochemistry 43:11717-26. 2004
    ..From these results, we present a model for a putative hinge domain in the context of recent "belt" and "hairpin" models of apoA-I structure in discoidal HDL particles...
  17. ncbi request reprint A three-dimensional molecular model of lipid-free apolipoprotein A-I determined by cross-linking/mass spectrometry and sequence threading
    R A Gangani D Silva
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Biochemistry 44:2759-69. 2005
    ..Furthermore, our data suggest that the self-association of lipid-free apoA-I occurs via C- and N-termini of the protein based on the locations of six cross-links that are unique to the cross-linked dimeric form of apoA-I...
  18. ncbi request reprint A mass spectrometric determination of the conformation of dimeric apolipoprotein A-I in discoidal high density lipoproteins
    R A Gangani D Silva
    Department of Pathology and Laboratory Medicine, The University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
    Biochemistry 44:8600-7. 2005
    ..A comparison of this work to a previous study is suggestive that a third molecule of apoA-I can form a hairpin in larger particles containing three molecules of apoA-I...
  19. ncbi request reprint Structure of human apolipoprotein A-IV: a distinct domain architecture among exchangeable apolipoproteins with potential functional implications
    Kevin Pearson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Biochemistry 43:10719-29. 2004
    ....
  20. ncbi request reprint The role of apolipoprotein A-I helix 10 in apolipoprotein-mediated cholesterol efflux via the ATP-binding cassette transporter ABCA1
    Stacey E Panagotopulos
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529, USA
    J Biol Chem 277:39477-84. 2002
    ..From these observations, we propose an alternative model for apolipoprotein-mediated efflux...
  21. pmc The role of hydrophobic and negatively charged surface patches of lipid-free apolipoprotein A-I in lipid binding and ABCA1-mediated cholesterol efflux
    Loren E Smith
    Department of Pathology and Laboratory Medicine, University of Cincinnati, 2120 East Galbraith Road, Cincinnati, OH 45237 0507, USA
    Biochim Biophys Acta 1801:64-9. 2010
    ..The hydrophobic surface patch may be important to the structural stability of lipid-free apoA-I or may be a necessary permissive structural element for lipid binding...
  22. ncbi request reprint Bacterial expression and characterization of rat apolipoprotein E
    Kevin Pearson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    Protein Expr Purif 41:447-53. 2005
    ..The recombinant protein was then compared both structurally and functionally to rat plasma apoE. This expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats...
  23. pmc Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease
    Matthew R Tubb
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
    J Lipid Res 50:1497-504. 2009
    ..In addition to cost-effectiveness, advantages of the tobacco etch virus protease include a short cleavage time, low reaction temperature, and easy removal using the protease's own histidine-tag...
  24. pmc Effect of intraperitoneal and intravenous administration of cholecystokinin-8 and apolipoprotein AIV on intestinal lymphatic CCK-8 and apo AIV concentration
    Chun Min Lo
    Cincinnati Obesity Research Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45237, USA
    Am J Physiol Regul Integr Comp Physiol 296:R43-50. 2009
    ....
  25. pmc Estradiol increases the anorectic effect of central apolipoprotein A-IV
    Ling Shen
    Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45237 0507, USA
    Endocrinology 151:3163-8. 2010
    ..These data indicate that an increased signaling of endogenous apo A-IV may partially mediate estradiol-induced inhibitory effect on feeding...
  26. ncbi request reprint Hypothalamic apolipoprotein A-IV is regulated by leptin
    Ling Shen
    Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45237 0507, USA
    Endocrinology 148:2681-9. 2007
    ..5 microg) reduction of feeding, indicating the existence of a functional synergistic interaction between leptin and apo A-IV, leading to suppression of food intake...
  27. ncbi request reprint Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation
    Kara E Schmid
    Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267, USA
    J Lipid Res 44:1909-18. 2003
    ..These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side)...
  28. ncbi request reprint Interaction of apolipoprotein AIV with cholecystokinin on the control of food intake
    Chun Min Lo
    Dept of Pathology and Laboratory Medicine, Univ of Cincinnati, 2120 E Galbraith Rd, Cincinnati, OH 45237 0507, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1490-4. 2007
    ....
  29. ncbi request reprint Apolipoprotein A-IV interacts synergistically with melanocortins to reduce food intake
    Koro Gotoh
    Department of Psychiatry, University of Cincinnati, 2170 East Galbraith Road, Cincinnati, OH 45237, USA
    Am J Physiol Regul Integr Comp Physiol 290:R202-7. 2006
    ..In addition, c-Fos expression was not colocalized with proopiomelanocortin-positive neurons. These data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate...
  30. ncbi request reprint Carboxyl ester lipase expression in macrophages increases cholesteryl ester accumulation and promotes atherosclerosis
    Ahmer Kodvawala
    Department of Pathology and Laboratory Medicine, Genome Research Institute, University of Cincinnati College of Medicine, Cincinnati, Ohio 45237, USA
    J Biol Chem 280:38592-8. 2005
    ....
  31. pmc Characterization of apolipoprotein A-IV in brain areas involved in energy homeostasis
    Ling Shen
    Obesity Research Center, University of Cincinnati College of Medicine, Cincinnati, OH 45237, USA
    Physiol Behav 95:161-7. 2008
    ..These data collectively support the hypothesis that apo A-IV, produced by neuronal cells, may exert its anorectic action by interacting with catabolic regulatory neuropeptides...
  32. pmc Enhanced placental cholesterol efflux by fetal HDL in Smith-Lemli-Opitz syndrome
    Katie T Jenkins
    Department of Pathology, Genome Research Institute, University of Cincinnati Medical School, 2180 E Galbraith Road, Cincinnati, OH 45237 0507, USA
    Mol Genet Metab 94:240-7. 2008
    ....
  33. ncbi request reprint Ceramide structural features required to stimulate ABCA1-mediated cholesterol efflux to apolipoprotein A-I
    Amy B Ghering
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
    J Lipid Res 47:2781-8. 2006
    ..These data show that the overall ceramide shape and the amide bond are critical for the cholesterol efflux effect and suggest that ceramide acts through a protein-mediated pathway to affect ABCA1 activity...
  34. ncbi request reprint Ceramide enhances cholesterol efflux to apolipoprotein A-I by increasing the cell surface presence of ATP-binding cassette transporter A1
    Scott R Witting
    Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529, USA
    J Biol Chem 278:40121-7. 2003
    ..These data suggest that ceramide may increase the plasma membrane content of ABCA1, leading to increased apoA-I binding and cholesterol efflux...
  35. pmc An amphipathic helical region of the N-terminal barrel of phospholipid transfer protein is critical for ABCA1-dependent cholesterol efflux
    John F Oram
    Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Box 356426, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 283:11541-9. 2008
    ..Direct interactions with ABCA1 and activation of signaling pathways likely involve other structural determinants of PLTP...
  36. ncbi request reprint Helix orientation of the functional domains in apolipoprotein e in discoidal high density lipoprotein particles
    Vasanthy Narayanaswami
    Lipid Biology in Health and Disease Research Group, Children s Hospital Oakland Research Institute, Oakland, California 94609, USA
    J Biol Chem 279:14273-9. 2004
    ..In this alignment, the residues of helix 4 are arrayed in a positively charged, curved helical segment for optimal receptor interaction...
  37. ncbi request reprint ABCA1-induced cell surface binding sites for ApoA-I
    Charulatha Vedhachalam
    Division of GI Nutrition, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4318, USA
    Arterioscler Thromb Vasc Biol 27:1603-9. 2007
    ..The purpose of this study was to understand the interactions of apoA-I with cells expressing ABCA1...
  38. pmc Apolipoprotein A-IV inhibits experimental colitis
    Thorsten Vowinkel
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport 71130 3932, USA
    J Clin Invest 114:260-9. 2004
    ..This anti-inflammatory effect likely involves the inhibition of P-selectin-mediated leukocyte and platelet adhesive interactions...

Research Grants22

  1. Apolipoprotein A-I structure in high density lipoproteins
    W Sean Davidson; Fiscal Year: 2010
    ....
  2. APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUX
    W Davidson; Fiscal Year: 2001
    ..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..
  3. Apolipoprotein-mediated cholesterol efflux
    W Davidson; Fiscal Year: 2007
    ..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..
  4. The roles of apolipoprotein A-IV structure and lipid affinity in its function
    W Davidson; Fiscal Year: 2007
    ....
  5. Apolipoprotein A-I structure in high density lipoproteins
    W Davidson; Fiscal Year: 2007
    ....
  6. Apolipoprotein A-I structure in high density lipoproteins
    W Davidson; Fiscal Year: 2009
    ....
  7. The roles of apolipoprotein A-IV structure and lipid affinity in its function
    W Davidson; Fiscal Year: 2009
    ....
  8. Roles of apolipoprotein A-IV structure/lipid affinity
    W Davidson; Fiscal Year: 2006
    ....
  9. Apolipoprotein-mediated cholesterol efflux
    W Davidson; Fiscal Year: 2006
    ..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..
  10. Apolipoprotein A-I Structure in High Density Lipoprotein
    W Davidson; Fiscal Year: 2005
    ..This information will provide a basis for highly targeted mutagenesis strategies resulting in apoA-I variants that can be used in vivo to dissect out the cardio-protective functions of apoA-I. ..
  11. APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUX
    W Davidson; Fiscal Year: 2000
    ..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..
  12. APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUX
    W Davidson; Fiscal Year: 2002
    ..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..
  13. Apolipoprotein A-I Structure in High Density Lipoprotein
    W Davidson; Fiscal Year: 2002
    ..This information will provide a basis for highly targeted mutagenesis strategies resulting in apoA-I variants that can be used in vivo to dissect out the cardio-protective functions of apoA-I. ..
  14. Apolipoprotein A-I Structure in High Density Lipoprotein
    W Davidson; Fiscal Year: 2003
    ..This information will provide a basis for highly targeted mutagenesis strategies resulting in apoA-I variants that can be used in vivo to dissect out the cardio-protective functions of apoA-I. ..
  15. APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUX
    W Davidson; Fiscal Year: 2003
    ..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..
  16. Apolipoprotein-mediated cholesterol efflux
    W Davidson; Fiscal Year: 2004
    ..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..
  17. Apolipoprotein A-I Structure in High Density Lipoprotein
    W Davidson; Fiscal Year: 2004
    ..This information will provide a basis for highly targeted mutagenesis strategies resulting in apoA-I variants that can be used in vivo to dissect out the cardio-protective functions of apoA-I. ..
  18. Apolipoprotein-mediated cholesterol efflux
    W Davidson; Fiscal Year: 2005
    ..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..
  19. APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUX
    W Davidson; Fiscal Year: 1999
    ..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..