Research Topics
Species | W DavidsonSummaryAffiliation: University of Cincinnati Country: USA Publications
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Publications
The structure of apolipoprotein A-II in discoidal high density lipoproteinsR A Gangani D Silva
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
J Biol Chem 282:9713-21. 2007..The data clearly refute a parallel model but support two antiparallel models, especially a "double hairpin" form. These models form the basis for understanding apoA-II structure in more complex HDL particles...- The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteinsW Sean Davidson
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
J Lipid Res 47:440-9. 2006..5 h, consuming only 120 microg of apolipoprotein in total. The benefits and potential drawbacks of the assay are discussed... 
Proteomic analysis of defined HDL subpopulations reveals particle-specific protein clusters: relevance to antioxidative functionW Sean Davidson
Department of Pathobiology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
Arterioscler Thromb Vasc Biol 29:870-6. 2009..We hypothesized that distinct clusters of protein components may distinguish between physicochemically-defined subpopulations of HDL particles, and that such clusters may exert specific biological function(s)...- The structure of apolipoprotein A-I in high density lipoproteinsW Sean Davidson
Department of Pathology and Laboratory Medicine, University of Cincinnati, OH 45237, USA
J Biol Chem 282:22249-53. 2007 - The spatial organization of apolipoprotein A-I on the edge of discoidal high density lipoprotein particles: a mass specrometry studyW Sean Davidson
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529
J Biol Chem 278:27199-207. 2003.... - Apolipoprotein structural organization in high density lipoproteins: belts, bundles, hinges and hairpinsW Sean Davidson
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
Curr Opin Lipidol 16:295-300. 2005..To summarize recent advances towards an understanding of the three-dimensional structures of the apolipoprotein components of HDL with a specific focus on high resolution models of apolipoprotein A-I... - 5th Annual International Conference on HDL Cholesterol: metabolic pathways and drug developmentsW Sean Davidson
Department of Pathology, University of Cincinnati Genome Research Institute, 2120 East Galbraith Road, Building A ML 0507, Cincinnati, OH 45237, USA
Expert Opin Ther Targets 8:359-66. 2004..A detailed understanding of the molecular basis for the protective effect of HDL will hopefully lead to the development of new therapeutics that exploit this pathway... - Apolipoprotein A-I alpha -helices 7 and 8 modulate high density lipoprotein subclass distributionErica J Reschly
Department of Pathology, University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 277:9645-54. 2002..Human apoA-I self-associates more and activates human lecithin-cholesterol acyltransferase better than mouse apoA-I. These differential characteristics of human and mouse apoA-I are not dependent on helices 7/8... - Specific sequences in the N and C termini of apolipoprotein A-IV modulate its conformation and lipid associationKevin Pearson
Department of Pathology and Laboratory Medicine, The University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
J Biol Chem 280:38576-82. 2005..We propose a structural model in which these sequences can modulate the conformation and lipid affinity of apoA-IV... - A three-dimensional homology model of lipid-free apolipoprotein A-IV using cross-linking and mass spectrometryMatthew R Tubb
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
J Biol Chem 283:17314-23. 2008..This first structural model of lipid-free apoA-IV should prove useful for designing studies aimed at understanding how apoA-IV interacts with lipids and possibly with unknown protein partners... - Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizesR A Gangani D Silva
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237, USA
Proc Natl Acad Sci U S A 105:12176-81. 2008..We propose the first experiment-based molecular model of apoA-I in spherical HDL particles. This model provides a new foundation for understanding how apoA-I structure modulates HDL function and metabolism... - Expression of biologically active rat apolipoprotein AIV in Escherichia coliMin Liu
Department of Pathology, University of Cincinnati School of Medicine, Cincinnati, OH 45267 0529, USA
Physiol Behav 78:149-55. 2003.... - Modulation of apolipoprotein A-IV lipid binding by an interaction between the N and C terminiMatthew R Tubb
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237, USA
J Biol Chem 282:28385-94. 2007..This interaction could represent a unique "switch" mechanism by which apoA-IV changes lipid avidity in vivo... - Proteomic characterization of human plasma high density lipoprotein fractionated by gel filtration chromatographyScott M Gordon
Center for Lipid and Arteriosclerosis Science, University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
J Proteome Res 9:5239-49. 2010..The observed heterogeneity across subfractions suggests the presence of HDL particle subpopulations each with distinct protein components that may prove to impart distinct physiological functions... - A three-dimensional molecular model of lipid-free apolipoprotein A-I determined by cross-linking/mass spectrometry and sequence threadingR A Gangani D Silva
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
Biochemistry 44:2759-69. 2005..Furthermore, our data suggest that the self-association of lipid-free apoA-I occurs via C- and N-termini of the protein based on the locations of six cross-links that are unique to the cross-linked dimeric form of apoA-I... - Identification and structural ramifications of a hinge domain in apolipoprotein A-I discoidal high-density lipoproteins of different sizeJ Nicholas Maiorano
Department of Pathology and Laboratory Medicine, University of Cincinnati, 2120 East Galbraith Road, Cincinnati, Ohio 45237 0507, USA
Biochemistry 43:11717-26. 2004..From these results, we present a model for a putative hinge domain in the context of recent "belt" and "hairpin" models of apoA-I structure in discoidal HDL particles... - The role of apolipoprotein A-I helix 10 in apolipoprotein-mediated cholesterol efflux via the ATP-binding cassette transporter ABCA1Stacey E Panagotopulos
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0529, USA
J Biol Chem 277:39477-84. 2002..From these observations, we propose an alternative model for apolipoprotein-mediated efflux... - The role of hydrophobic and negatively charged surface patches of lipid-free apolipoprotein A-I in lipid binding and ABCA1-mediated cholesterol effluxLoren E Smith
Department of Pathology and Laboratory Medicine, University of Cincinnati, 2120 East Galbraith Road, Cincinnati, OH 45237 0507, USA
Biochim Biophys Acta 1801:64-9. 2010..The hydrophobic surface patch may be important to the structural stability of lipid-free apoA-I or may be a necessary permissive structural element for lipid binding... - A mass spectrometric determination of the conformation of dimeric apolipoprotein A-I in discoidal high density lipoproteinsR A Gangani D Silva
Department of Pathology and Laboratory Medicine, The University of Cincinnati, Cincinnati, Ohio 45237 0507, USA
Biochemistry 44:8600-7. 2005..A comparison of this work to a previous study is suggestive that a third molecule of apoA-I can form a hairpin in larger particles containing three molecules of apoA-I... - Structure of human apolipoprotein A-IV: a distinct domain architecture among exchangeable apolipoproteins with potential functional implicationsKevin Pearson
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
Biochemistry 43:10719-29. 2004.... - Effect of intraperitoneal and intravenous administration of cholecystokinin-8 and apolipoprotein AIV on intestinal lymphatic CCK-8 and apo AIV concentrationChun Min Lo
Cincinnati Obesity Research Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45237, USA
Am J Physiol Regul Integr Comp Physiol 296:R43-50. 2009.... - Bacterial expression and characterization of rat apolipoprotein EKevin Pearson
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
Protein Expr Purif 41:447-53. 2005..The recombinant protein was then compared both structurally and functionally to rat plasma apoE. This expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats... - Estradiol increases the anorectic effect of central apolipoprotein A-IVLing Shen
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45237 0507, USA
Endocrinology 151:3163-8. 2010..These data indicate that an increased signaling of endogenous apo A-IV may partially mediate estradiol-induced inhibitory effect on feeding... - Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus proteaseMatthew R Tubb
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237 0507, USA
J Lipid Res 50:1497-504. 2009..In addition to cost-effectiveness, advantages of the tobacco etch virus protease include a short cleavage time, low reaction temperature, and easy removal using the protease's own histidine-tag... - Carboxyl ester lipase expression in macrophages increases cholesteryl ester accumulation and promotes atherosclerosisAhmer Kodvawala
Department of Pathology and Laboratory Medicine, Genome Research Institute, University of Cincinnati College of Medicine, Cincinnati, Ohio 45237, USA
J Biol Chem 280:38592-8. 2005.... - Interaction of apolipoprotein AIV with cholecystokinin on the control of food intakeChun Min Lo
Dept of Pathology and Laboratory Medicine, Univ of Cincinnati, 2120 E Galbraith Rd, Cincinnati, OH 45237 0507, USA
Am J Physiol Regul Integr Comp Physiol 293:R1490-4. 2007.... - Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulationKara E Schmid
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267, USA
J Lipid Res 44:1909-18. 2003..These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side)... - Apolipoprotein A-IV interacts synergistically with melanocortins to reduce food intakeKoro Gotoh
Department of Psychiatry, University of Cincinnati, 2170 East Galbraith Road, Cincinnati, OH 45237, USA
Am J Physiol Regul Integr Comp Physiol 290:R202-7. 2006..In addition, c-Fos expression was not colocalized with proopiomelanocortin-positive neurons. These data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate... - Hypothalamic apolipoprotein A-IV is regulated by leptinLing Shen
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45237 0507, USA
Endocrinology 148:2681-9. 2007..5 microg) reduction of feeding, indicating the existence of a functional synergistic interaction between leptin and apo A-IV, leading to suppression of food intake... - Characterization of apolipoprotein A-IV in brain areas involved in energy homeostasisLing Shen
Obesity Research Center, University of Cincinnati College of Medicine, Cincinnati, OH 45237, USA
Physiol Behav 95:161-7. 2008..These data collectively support the hypothesis that apo A-IV, produced by neuronal cells, may exert its anorectic action by interacting with catabolic regulatory neuropeptides... - Enhanced placental cholesterol efflux by fetal HDL in Smith-Lemli-Opitz syndromeKatie T Jenkins
Department of Pathology, Genome Research Institute, University of Cincinnati Medical School, 2180 E Galbraith Road, Cincinnati, OH 45237 0507, USA
Mol Genet Metab 94:240-7. 2008.... - Ceramide enhances cholesterol efflux to apolipoprotein A-I by increasing the cell surface presence of ATP-binding cassette transporter A1Scott R Witting
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0529, USA
J Biol Chem 278:40121-7. 2003..These data suggest that ceramide may increase the plasma membrane content of ABCA1, leading to increased apoA-I binding and cholesterol efflux... - Ceramide structural features required to stimulate ABCA1-mediated cholesterol efflux to apolipoprotein A-IAmy B Ghering
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237-0507, USA
J Lipid Res 47:2781-8. 2006..These data show that the overall ceramide shape and the amide bond are critical for the cholesterol efflux effect and suggest that ceramide acts through a protein-mediated pathway to affect ABCA1 activity... - Helix orientation of the functional domains in apolipoprotein e in discoidal high density lipoprotein particlesVasanthy Narayanaswami
Lipid Biology in Health and Disease Research Group, Children s Hospital Oakland Research Institute, Oakland, California 94609, USA
J Biol Chem 279:14273-9. 2004..In this alignment, the residues of helix 4 are arrayed in a positively charged, curved helical segment for optimal receptor interaction... - An amphipathic helical region of the N-terminal barrel of phospholipid transfer protein is critical for ABCA1-dependent cholesterol effluxJohn F Oram
Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Box 356426, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 283:11541-9. 2008..Direct interactions with ABCA1 and activation of signaling pathways likely involve other structural determinants of PLTP... - ABCA1-induced cell surface binding sites for ApoA-ICharulatha Vedhachalam
Division of GI Nutrition, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4318, USA
Arterioscler Thromb Vasc Biol 27:1603-9. 2007..The purpose of this study was to understand the interactions of apoA-I with cells expressing ABCA1... - Apolipoprotein A-IV inhibits experimental colitisThorsten Vowinkel
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport 71130 3932, USA
J Clin Invest 114:260-9. 2004..This anti-inflammatory effect likely involves the inhibition of P-selectin-mediated leukocyte and platelet adhesive interactions...
Research Grants
- Apolipoprotein-mediated cholesterol effluxW Davidson; Fiscal Year: 2007..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..
- The roles of apolipoprotein A-IV structure and lipid affinity in its functionW Davidson; Fiscal Year: 2007....
- Apolipoprotein A-I structure in high density lipoproteinsW Davidson; Fiscal Year: 2007....
- The roles of apolipoprotein A-IV structure and lipid affinity in its functionW Davidson; Fiscal Year: 2009....
- Apolipoprotein A-I Structure in High Density LipoproteinW Davidson; Fiscal Year: 2005..This information will provide a basis for highly targeted mutagenesis strategies resulting in apoA-I variants that can be used in vivo to dissect out the cardio-protective functions of apoA-I. ..
- APOLIPOPROTEIN-MEDIATED CHOLESTEROL EFFLUXW Davidson; Fiscal Year: 2003..Finally, the effect of those modifications on the ability of apoAI to promote cholesterol removal from cells will be determined in cell culture-based studies. ..
- Apolipoprotein A-I structure in high density lipoproteinsW Sean Davidson; Fiscal Year: 2010....