Richard Darveau

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts
    Thanuja D K Herath
    Faculty of Dentistry, Periodontology, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR, China
    BMC Microbiol 13:73. 2013
  2. pmc Porphyromonas gingivalis as a potential community activist for disease
    R P Darveau
    University of Washington, 1959 NE Pacific Street, Room D 570, UW Mailbox 357444, Seattle, WA 98195 7444, USA
    J Dent Res 91:816-20. 2012
  3. doi request reprint Periodontitis: a polymicrobial disruption of host homeostasis
    Richard P Darveau
    University of Washington, Seattle, Washington 98195, USA
    Nat Rev Microbiol 8:481-90. 2010
  4. pmc The oral microbial consortium's interaction with the periodontal innate defense system
    Richard P Darveau
    Department of Periodontics, University of Washington, Seattle, 98195 7444, USA
    DNA Cell Biol 28:389-95. 2009
  5. pmc Local chemokine paralysis, a novel pathogenic mechanism for Porphyromonas gingivalis
    R P Darveau
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA
    Infect Immun 66:1660-5. 1998
  6. pmc Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4
    Richard P Darveau
    Department of Periodontics, University of Washington, Health Sciences Center, Box 357444, Seattle, WA 98195, USA
    Infect Immun 72:5041-51. 2004
  7. pmc Porphyromonas gingivalis lipopolysaccharide is both agonist and antagonist for p38 mitogen-activated protein kinase activation
    Richard P Darveau
    Department of Periodontics, University of Washington, Seattle, Washington 98181, USA
    Infect Immun 70:1867-73. 2002
  8. ncbi request reprint Lipid A diversity and the innate host response to bacterial infection
    R P Darveau
    Department of Periodontics, Box 357444, School of Dentistry, Universityof Washington, Seattle, WA 98195 7444, USA
    Curr Opin Microbiol 1:36-42. 1998
  9. ncbi request reprint Porphyromonas gingivalis lipopolysaccharide displays functionally diverse interactions with the innate host defense system
    Brian W Bainbridge
    Department of Periodontics, University of Washington, Seattle, Washington 98195, USA
    Ann Periodontol 7:29-37. 2002
  10. ncbi request reprint Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity: differential activities of tetra- and penta-acylated lipid A structures on E-selectin expression and TLR4 recognition
    Robert A Reife
    Department of Periodontics, University of Washington, Seattle, WA 98195, USA
    Cell Microbiol 8:857-68. 2006

Collaborators

Detail Information

Publications29

  1. pmc The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts
    Thanuja D K Herath
    Faculty of Dentistry, Periodontology, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR, China
    BMC Microbiol 13:73. 2013
    ..gingivalis LPS1435/1449 and LPS1690 and hexa-acylated E. coli LPS as a reference. The expression of MMPs 1-3 and TIMP-1 was evaluated by real-time PCR and ELISA...
  2. pmc Porphyromonas gingivalis as a potential community activist for disease
    R P Darveau
    University of Washington, 1959 NE Pacific Street, Room D 570, UW Mailbox 357444, Seattle, WA 98195 7444, USA
    J Dent Res 91:816-20. 2012
    ..Thus, P. gingivalis creates a dysbiosis between the host and dental plaque, and this may represent one mechanism by which periodontitis can be initiated. We have therefore termed P. gingivalis a keystone pathogen...
  3. doi request reprint Periodontitis: a polymicrobial disruption of host homeostasis
    Richard P Darveau
    University of Washington, Seattle, Washington 98195, USA
    Nat Rev Microbiol 8:481-90. 2010
    ..Furthermore, disease may result from 'community-based' attack on the host. Here, I describe the interaction of the host immune system with the oral bacteria in healthy states and in diseased states...
  4. pmc The oral microbial consortium's interaction with the periodontal innate defense system
    Richard P Darveau
    Department of Periodontics, University of Washington, Seattle, 98195 7444, USA
    DNA Cell Biol 28:389-95. 2009
    ....
  5. pmc Local chemokine paralysis, a novel pathogenic mechanism for Porphyromonas gingivalis
    R P Darveau
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA
    Infect Immun 66:1660-5. 1998
    ..gingivalis colonization (local chemokine paralysis) will severely impair mucosal defense and represents a novel mechanism for bacterial colonization of host tissue...
  6. pmc Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4
    Richard P Darveau
    Department of Periodontics, University of Washington, Health Sciences Center, Box 357444, Seattle, WA 98195, USA
    Infect Immun 72:5041-51. 2004
    ..gingivalis LPS preparations is not currently understood, it is proposed that the presence of multiple lipid A species contributes to cell activation through both TLR2 and TLR4...
  7. pmc Porphyromonas gingivalis lipopolysaccharide is both agonist and antagonist for p38 mitogen-activated protein kinase activation
    Richard P Darveau
    Department of Periodontics, University of Washington, Seattle, Washington 98181, USA
    Infect Immun 70:1867-73. 2002
    ..These data also suggest that although LPS is generally considered a bacterial component that alerts the host to infection, LPS from P. gingivalis may selectively modify the host response as a means to facilitate colonization...
  8. ncbi request reprint Lipid A diversity and the innate host response to bacterial infection
    R P Darveau
    Department of Periodontics, Box 357444, School of Dentistry, Universityof Washington, Seattle, WA 98195 7444, USA
    Curr Opin Microbiol 1:36-42. 1998
    ..Recent data indicate that bacteria can regulate this molecule in response to different host microenvironments. Host factors that induce lipid A modifications and the resultant changes in host response remain to be determined...
  9. ncbi request reprint Porphyromonas gingivalis lipopolysaccharide displays functionally diverse interactions with the innate host defense system
    Brian W Bainbridge
    Department of Periodontics, University of Washington, Seattle, Washington 98195, USA
    Ann Periodontol 7:29-37. 2002
    ..We speculate that P. gingivalis lipid A structural heterogeneity contributes to the unusual innate host response to this LPS and its ability to interact with different TLR molecules...
  10. ncbi request reprint Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity: differential activities of tetra- and penta-acylated lipid A structures on E-selectin expression and TLR4 recognition
    Robert A Reife
    Department of Periodontics, University of Washington, Seattle, WA 98195, USA
    Cell Microbiol 8:857-68. 2006
    ..gingivalis LPS, with opposing effects on the E-selectin response suggests that this organism is able to modulate innate host responses by alterations in the relative amount of these lipid A structures...
  11. ncbi request reprint Expression of a Porphyromonas gingivalis lipid A palmitylacyltransferase in Escherichia coli yields a chimeric lipid A with altered ability to stimulate interleukin-8 secretion
    Brian W Bainbridge
    Department of Oral Biology, University of Washington, Seattle, WA 98195, USA
    Cell Microbiol 8:120-9. 2006
    ..coli lipid A altered the activity of the LPS in monocytes but not endothelial cell assays and the difference in recognition does not appear to be related to differences in Toll-like receptor utilization...
  12. ncbi request reprint Correlates of periodontal decline and biologic markers in older adults
    Jessica R Swoboda
    Department of Periodontics, University of Washington, Seattle, WA, USA
    J Periodontol 79:1920-6. 2008
    ..This study measured bacterial and serum cytokine and high-sensitivity C-reactive protein (hsCRP) levels in older persons...
  13. pmc Porphyromonas gingivalis resistance to polymyxin B is determined by the lipid A 4'-phosphatase, PGN_0524
    Stephen R Coats
    Department of Periodontics, School of Dentistry, University of Washington, Seattle, Washington 98195, USA
    Int J Oral Sci 1:126-35. 2009
    ..To elucidate the genetic basis for the pronounced resistance that the oral pathogen, Porphyromonas gingivalis (P. gingivalis), exhibits towards the cationic antimicrobial peptide, polymyxin B...
  14. pmc Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1- and 4'-phosphatase activities
    Stephen R Coats
    Department of Periodontics, School of Dentistry, University of Washington, 1959 NE Pacific St, Seattle, WA 98195 7444, USA
    Cell Microbiol 11:1587-99. 2009
    ..gingivalis lipid A activity from TLR4 evasive to TLR4 suppressive, potentially altering critical interactions between this bacterium, the local microbial community and the host innate immune system...
  15. pmc Porphyromonas gingivalis lipopolysaccharide antagonizes Escherichia coli lipopolysaccharide at toll-like receptor 4 in human endothelial cells
    Stephen R Coats
    Department of Periodontics, University of Washington, Seattle, Washington 98195, USA
    Infect Immun 71:6799-807. 2003
    ..gingivalis LPS-dependent antagonism of E. coli LPS in human endothelial cells likely involves the ability of P. gingivalis LPS to directly compete with E. coli LPS at the TLR4 signaling complex...
  16. ncbi request reprint Antagonistic lipopolysaccharides block E. coli lipopolysaccharide function at human TLR4 via interaction with the human MD-2 lipopolysaccharide binding site
    Stephen R Coats
    Department of Periodontics, University of Washington School of Dentistry, Seattle, WA 98195, USA
    Cell Microbiol 9:1191-202. 2007
    ..coli lipopolysaccharide-hMD-2 complexes function at hTLR4. It is also shown that both hTLR4 and hMD-2 contribute to the species-specific recognition of msbB and P. gingivalis lipopolysaccharides as antagonists at the hTLR4 complex...
  17. ncbi request reprint Modulation of the innate immune response within the periodontium
    Douglas R Dixon
    United States Army Dental Corps and Department of Periodontics and Oral Biology, School of Dentistry, University of Washington, Seattle, USA
    Periodontol 2000 35:53-74. 2004
  18. pmc The lipid A phosphate position determines differential host Toll-like receptor 4 responses to phylogenetically related symbiotic and pathogenic bacteria
    Stephen R Coats
    Department of Periodontics, School of Dentistry, University of Washington, 1959 NE Pacific St, HSB, Box 357444, Seattle, WA 98195, USA
    Infect Immun 79:203-10. 2011
    ..We propose that the distinct lipid A phosphate positions observed for the B. thetaiotaomicron and P. gingivalis LPS contributes to the manifestation of these bacteria as commensal or pathogen within the human host...
  19. ncbi request reprint MD-2 mediates the ability of tetra-acylated and penta-acylated lipopolysaccharides to antagonize Escherichia coli lipopolysaccharide at the TLR4 signaling complex
    Stephen R Coats
    Department of Periodontics, University of Washington School of Dentistry, Seattle 98195, USA
    J Immunol 175:4490-8. 2005
    ..However, MD-2 represents the principal molecular component that tetra-acylated P. gingivalis LPS and penta-acylated msbB LPS use to antagonize hexa-acylated E. coli LPS at the TLR4 signaling complex...
  20. ncbi request reprint Oral and serum IL-6 levels in oral lichen planus patients
    Gao Man Gu
    Dept of Orthodontics, School of Dentistry, University of Washington, Seattle, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98:673-8. 2004
    ..Venous blood was processed to serum for ELISA assay. Oral IL-6 was expressed as both pg/mL and pg/mug protein, and serum IL-6 was expressed as pg/mL...
  21. ncbi request reprint Hierarchical gene expression profiles of HUVEC stimulated by different lipid A structures obtained from Porphyromonas gingivalis and Escherichia coli
    Casey Chen
    Department of Periodontics and Oral Biology, University of Washington, Seattle, WA 98195, USA
    Cell Microbiol 9:1028-38. 2007
    ..A unique gene expression profile for the weak TLR4 agonist PgLPS(1690) was observed and represents a TLR4 hierarchy in endothelial cell gene activation...
  22. pmc The structurally similar, penta-acylated lipopolysaccharides of Porphyromonas gingivalis and Bacteroides elicit strikingly different innate immune responses
    Alex B Berezow
    Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Microb Pathog 47:68-77. 2009
    ..Further, we show the difference in potency between Bacteroides and P. gingivalis LPS is TLR4-dependent. Altogether, the data suggest subtle changes in lipid A structure may profoundly impact the host's innate immune response...
  23. pmc Hemin-dependent modulation of the lipid A structure of Porphyromonas gingivalis lipopolysaccharide
    Montaser N Al-Qutub
    Department of Periodontics, University of Washington, Box 357444, Seattle, Washington 98195, USA
    Infect Immun 74:4474-85. 2006
    ..gingivalis lipid A structures have opposing effects on Toll-like receptor 4 activation, the alteration of the lipid A structural content may have significant effects on the host response to this bacterium...
  24. pmc Acyl chain specificity of the acyltransferases LpxA and LpxD and substrate availability contribute to lipid A fatty acid heterogeneity in Porphyromonas gingivalis
    Brian W Bainbridge
    Department of Periodontics and Oral Biology and Medicine, University of Washington, D 652 Health Sciences Building, 1959 NE Pacific St, Seattle, Washington 98195 7444, USA
    J Bacteriol 190:4549-58. 2008
    ..gingivalis lipid A acyltransferases and the substrate availability account for the lipid A structural clusters that differ by 14 mass units observed in P. gingivalis lipopolysaccharide preparations...
  25. doi request reprint Antimicrobial photodynamic therapy may promote periodontal healing through multiple mechanisms
    Pam Braham
    Department of Periodontics, University of Washington, Seattle, WA 98195 7444, USA
    J Periodontol 80:1790-8. 2009
    ..It also inactivates host destructive cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1 beta...
  26. pmc P. gingivalis and E. coli lipopolysaccharides exhibit different systemic but similar local induction of inflammatory markers
    Rongkun Liu
    Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, MA 02118, USA
    J Periodontol 79:1241-7. 2008
    ..gingivalis lipopolysaccharide (PgLPS), PgLPS(1435)(/1449) and PgLPS(1690), exhibit differences in their capacity to stimulate systemic versus local responses compared to Escherichia coli lipopolysaccharide (LPS)...
  27. ncbi request reprint The expression profile of lipopolysaccharide-binding protein, membrane-bound CD14, and toll-like receptors 2 and 4 in chronic periodontitis
    Lei Ren
    Faculty of Dentistry, Periodontology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Periodontol 76:1950-9. 2005
    ....
  28. pmc Mouse paneth cell secretory responses to cell surface glycolipids of virulent and attenuated pathogenic bacteria
    Hiroki Tanabe
    Department of Pathology, School of Medicine, College of Health Sciences, University of California, Irvine, CA 92697 4800, USA
    Infect Immun 73:2312-20. 2005
    ..Thus, mouse Paneth cells respond equivalently to purified bacterial cell envelope glycolipids, regardless of functional Tlr4, the structural properties of glycolipid acyl chains, or their association with virulence in humans...
  29. ncbi request reprint The in vivo expression of membrane-bound CD14 in periodontal health and disease
    Lijian Jin
    Faculty of Dentistry, Periodontology, The University of Hong Kong, Hong Kong
    J Periodontol 75:578-85. 2004
    ..This study investigated the in vivo expression profile and levels of mCD14 in healthy and diseased gingival tissues...

Research Grants21

  1. Oral Commensal Bacterial Modulation of the Periodontal Innate Host Response
    Richard Darveau; Fiscal Year: 2007
    ....
  2. P Gingivalis LPS: Hemin-induced lipid A structural remodelling
    RICHARD PETERS DARVEAU; Fiscal Year: 2010
    ..We suspect the modulation of the host immune response contributes to its ability to cause disease. ..
  3. P. GINGIVALIS LPS-MODULATION OF INNATE HOST DEFENSE
    Richard Darveau; Fiscal Year: 2007
    ..gingivalis lipid A diversity is generated. The third Aim will examine potential TLR mediated innate host interactions of the different lipid A species generated in Aims 1 and 2. ..
  4. P. gingivalis lipid A species modulation of endothelial cell gene activation prog
    Richard Darveau; Fiscal Year: 2007
    ..gingivalis interactions with TLR4 or other novel LPS receptors. Furthermore, the microarray analysis results will be made public by deposition at the European Bioinformatics Institute Array Express database. ..
  5. Oral Commensal Bacterial Modulation of the Periodontal Innate Host Response
    RICHARD PETERS DARVEAU; Fiscal Year: 2010
    ....
  6. ASM Conf. on Beneficial Microbial Symbionts in Animals
    Richard Darveau; Fiscal Year: 2005
    ..It is an intellectually stimulating subject that attracts pioneering graduate students and post-doctoral fellows who will benefit from participation in a focused and dynamic conference; ..
  7. LBP/CD14 interactions with bacterial components
    Richard Darveau; Fiscal Year: 2005
    ..These studies will provide further insight into how the innate host defense system recognizes and responds to different bacteria, a key component of both oral health and disease. ..
  8. PGINGIVALIS LPS--INHIBITION OF INNATE HOST DEFENSE
    Richard Darveau; Fiscal Year: 2002
    ..The overall goal of this application is to identify new therapies based upon this unique virulence characteristic of P. gingivalis LPS. ..