Douglas S Darling

Summary

Affiliation: University of Louisville
Country: USA

Publications

  1. pmc Expression of Zfhep/deltaEF1 protein in palate, neural progenitors, and differentiated neurons
    Douglas S Darling
    Periodontics, Endodontics and Dental Hygiene, University of Louisville School of Dentistry, Health Sciences Center, 501 South Preston Street, Louisville, KY 40292, USA
    Gene Expr Patterns 3:709-17. 2003
  2. pmc Zeb1 represses Mitf and regulates pigment synthesis, cell proliferation, and epithelial morphology
    Yongqing Liu
    James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA
    Invest Ophthalmol Vis Sci 50:5080-8. 2009
  3. pmc Analysis of Zfhx1a mutant mice reveals palatal shelf contact-independent medial edge epithelial differentiation during palate fusion
    Jiu Zhen Jin
    Department of Molecular, Cellular and Craniofacial Biology, University of Louisville, Louisville, KY 40202, USA
    Cell Tissue Res 333:29-38. 2008
  4. pmc Zeb1 mutant mice as a model of posterior corneal dystrophy
    Yongqing Liu
    Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, 301 E Muhammad Ali Boulevard, Louisville, KY 40202, USA
    Invest Ophthalmol Vis Sci 49:1843-9. 2008
  5. pmc Zeb1 links epithelial-mesenchymal transition and cellular senescence
    Yongqing Liu
    James Graham Brown Cancer Center, Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Development 135:579-88. 2008
  6. pmc The zinc finger transcription factor ZFHX1A is linked to cell proliferation by Rb-E2F1
    Yongqing Liu
    James Graham Brown Cancer Center, Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Biochem J 408:79-85. 2007
  7. pmc Combination of a zinc finger and homeodomain required for protein-interaction
    Gregory E Smith
    Biochemistry and Molecular Biology, Center for Genetics and Molecular Medicine, University of Louisville Health Sciences Center, Louisville, KY 40292, USA
    Mol Biol Rep 30:199-206. 2003
  8. ncbi request reprint Validation of a mathematical model of gene transcription in aggregated cellular systems: application to l1 retrotransposition
    Grzegorz A Rempala
    Department of Mathematics, University of Louisville, Louisville, Kentucky 40292, USA
    J Comput Biol 14:339-49. 2007
  9. ncbi request reprint A stochastic model of gene transcription: an application to L1 retrotransposition events
    Grzegorz A Rempala
    Department of Mathematics, University of Louisville, Louisville, KY 40292, USA
    J Theor Biol 242:101-16. 2006
  10. pmc Cell-specific phosphorylation of Zfhep transcription factor
    Mary E Costantino
    Department of Biochemistry and Molecular Biology, University of Louisville Health Sciences Center, 501 South Preston St, Room 315, Louisville, KY 40292, USA
    Biochem Biophys Res Commun 296:368-73. 2002

Research Grants

  1. Mathematical Model of Parotid Acinar Differentiation
    Douglas Darling; Fiscal Year: 2009
  2. Ocular defects caused by TCF8/Zfhx1a mutations
    Douglas Darling; Fiscal Year: 2007
  3. SELECTIVE AGGREGATION AND SORTING OF SALIVARY PROTEINS
    Douglas Darling; Fiscal Year: 2007
  4. Identifying Periodontal Antigens By Protein Microarray
    Douglas Darling; Fiscal Year: 2006
  5. TRANSCRIPTION FACTORS REGULATING PALATOGENESIS
    Douglas Darling; Fiscal Year: 2002
  6. Mathematical Model of Parotid Acinar Differentiation
    Grzegorz A Rempala; Fiscal Year: 2010

Collaborators

  • Kenneth Ramos
  • Grzegorz A Rempala
  • Qun Li
  • Fei Ye
  • Yujiro Higashi
  • Yongqing Liu
  • Douglas C Dean
  • Mary E Costantino
  • Gregory E Smith
  • Henry J Kaplan
  • Meenakshi Singh
  • Jiu Zhen Jin
  • Takanori Kowase
  • Pablo A Manavella
  • Shigeo Tamiya
  • Kathryn B Horwitz
  • Erin Howe
  • Jinlian Tan
  • Kenneth R Shroyer
  • Xiaoyan Peng
  • Hilda R Clark
  • Jixiang Ding
  • Jennifer K Richer
  • Shahenda El-Naggar
  • Russell R Broaddus
  • Nicole S Spoelstra
  • Kathleen C Torkko
  • Annie Jean
  • Margaret A Shupnik
  • Ana M Cabanillas
  • Heidi E Walsh
  • Diego Montoya-Durango
  • Gonzalo Roqueiro
  • Randi P Stearman

Detail Information

Publications13

  1. pmc Expression of Zfhep/deltaEF1 protein in palate, neural progenitors, and differentiated neurons
    Douglas S Darling
    Periodontics, Endodontics and Dental Hygiene, University of Louisville School of Dentistry, Health Sciences Center, 501 South Preston Street, Louisville, KY 40292, USA
    Gene Expr Patterns 3:709-17. 2003
    ..In vitro studies have implicated Zfhep as a repressor of myogenesis, however, we find that Zfhep protein expression increases during muscle differentiation...
  2. pmc Zeb1 represses Mitf and regulates pigment synthesis, cell proliferation, and epithelial morphology
    Yongqing Liu
    James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA
    Invest Ophthalmol Vis Sci 50:5080-8. 2009
    ..The purpose of this study was to investigate the potential role for Zeb1 in EMT leading to the dedifferentiation of retinal pigment epithelial (RPE) cells...
  3. pmc Analysis of Zfhx1a mutant mice reveals palatal shelf contact-independent medial edge epithelial differentiation during palate fusion
    Jiu Zhen Jin
    Department of Molecular, Cellular and Craniofacial Biology, University of Louisville, Louisville, KY 40202, USA
    Cell Tissue Res 333:29-38. 2008
    ..5 in a contact-independent manner, suggesting that differentiation of the medial edge epithelium was largely programmed through an intrinsic mechanism within the palate shelf...
  4. pmc Zeb1 mutant mice as a model of posterior corneal dystrophy
    Yongqing Liu
    Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, 301 E Muhammad Ali Boulevard, Louisville, KY 40202, USA
    Invest Ophthalmol Vis Sci 49:1843-9. 2008
    ..The purpose of this study is to determine whether Zeb1 null or heterozygous mice may provide an animal model for PPCD...
  5. pmc Zeb1 links epithelial-mesenchymal transition and cellular senescence
    Yongqing Liu
    James Graham Brown Cancer Center, Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Development 135:579-88. 2008
    ..We demonstrate that this ectopic expression of p15Ink4b extends in vivo to sites of diminished progenitor cell proliferation and developmental defects in Zeb1-null mice...
  6. pmc The zinc finger transcription factor ZFHX1A is linked to cell proliferation by Rb-E2F1
    Yongqing Liu
    James Graham Brown Cancer Center, Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Biochem J 408:79-85. 2007
    ..We propose that the differential effects of the Rb family/E2F pathway on expression of these E-box-binding proteins are important in maintaining their distinct patterns (and thus distinct functions) during embryogenesis...
  7. pmc Combination of a zinc finger and homeodomain required for protein-interaction
    Gregory E Smith
    Biochemistry and Molecular Biology, Center for Genetics and Molecular Medicine, University of Louisville Health Sciences Center, Louisville, KY 40292, USA
    Mol Biol Rep 30:199-206. 2003
    ..This is the first demonstration of a function for the HD region of Zfhep, which is the first case of a bi-partite domain requiring both a zinc finger and a HD for binding to protein...
  8. ncbi request reprint Validation of a mathematical model of gene transcription in aggregated cellular systems: application to l1 retrotransposition
    Grzegorz A Rempala
    Department of Mathematics, University of Louisville, Louisville, Kentucky 40292, USA
    J Comput Biol 14:339-49. 2007
    ..The procedure compares model predicted values with the RT-PCR data for L1 by means of the standard Bayesian statistical techniques with the help of modern Markov-Chain Monte-Carlo methodology...
  9. ncbi request reprint A stochastic model of gene transcription: an application to L1 retrotransposition events
    Grzegorz A Rempala
    Department of Mathematics, University of Louisville, Louisville, KY 40292, USA
    J Theor Biol 242:101-16. 2006
    ..The procedure compares model predicted values with the laboratory data via the standard Bayesian techniques with the help of modern Markov-Chain Monte-Carlo methodology...
  10. pmc Cell-specific phosphorylation of Zfhep transcription factor
    Mary E Costantino
    Department of Biochemistry and Molecular Biology, University of Louisville Health Sciences Center, 501 South Preston St, Room 315, Louisville, KY 40292, USA
    Biochem Biophys Res Commun 296:368-73. 2002
    ..These data provide the first demonstration that Zfhep is post-translationally modified...
  11. pmc The ZFHX1A gene is differentially autoregulated by its isoforms
    Pablo A Manavella
    CIBICI CONICET, Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Haya de la Torre y Medina Allende, 5000 Cordoba, Argentina
    Biochem Biophys Res Commun 360:621-6. 2007
    ..Hence, the Zfhx1a-1 gene is autoregulated in part by negative feedback on its own promoter which is, in turn, modified by the availability of the negative dominant isoform Zfhx1a-2...
  12. ncbi request reprint Estrogen enhances gonadotropin-releasing hormone-stimulated transcription of the luteinizing hormone subunit promoters via altered expression of stimulatory and suppressive transcription factors
    Takanori Kowase
    Division of Endocrinology, Department of Medicine, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Endocrinology 148:6083-91. 2007
    ..Thus, one mechanism by which E may enhance GnRH-stimulated LH subunit promoter activity is through regulation of both activators and suppressors of transcription...
  13. doi request reprint ZEB1 expression in type I vs type II endometrial cancers: a marker of aggressive disease
    Meenakshi Singh
    Department of Pathology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    Mod Pathol 21:912-23. 2008
    ..It may help identify women who would most benefit from chemotherapy. Furthermore, if expression of ZEB1 in type II endometrial cancers could be reversed, it might be exploited as therapy for these highly aggressive tumors...

Research Grants15

  1. Mathematical Model of Parotid Acinar Differentiation
    Douglas Darling; Fiscal Year: 2009
    ..This research is a necessary foundation for developing new technologies such as gene transfer therapy and biologics for treating or alleviating the oral symptoms of xerostomia. ..
  2. Ocular defects caused by TCF8/Zfhx1a mutations
    Douglas Darling; Fiscal Year: 2007
    ..A very early therapeutic treatment is needed to prevent later secondary defects. This directly relates to the National Eye Institute program's goal to investigate the development of the visual system. ..
  3. SELECTIVE AGGREGATION AND SORTING OF SALIVARY PROTEINS
    Douglas Darling; Fiscal Year: 2007
    ..This will lead to a better understanding of the formation of saliva and provide the basis for efficient delivery of therapeutic proteins from salivary glands. ..
  4. Identifying Periodontal Antigens By Protein Microarray
    Douglas Darling; Fiscal Year: 2006
    ..The R21 Exploratory Grant is an appropriate mechanism since proteomics have not been used for periodontal disease, and bacterial genomic expression libraries and antigen microarrays need to be developed. ..
  5. TRANSCRIPTION FACTORS REGULATING PALATOGENESIS
    Douglas Darling; Fiscal Year: 2002
    ..Subsequent work will investigate the molecular mechanism(s) of Zfhep action during craniofacial development. ..
  6. Mathematical Model of Parotid Acinar Differentiation
    Grzegorz A Rempala; Fiscal Year: 2010
    ..This research is a necessary foundation for developing new technologies such as gene transfer therapy and biologics for treating or alleviating the oral symptoms of xerostomia. ..