M T Cushion

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. pmc Assembly and annotation of Pneumocystis jirovecii from the human lung microbiome
    Melanie T Cushion
    University of Cincinnati, College of Medicine, Cincinnati, Ohio, USA
    MBio 4:e00224. 2013
  2. ncbi request reprint Preclinical drug discovery for new anti-pneumocystis compounds
    Melanie T Cushion
    Research Service 151, VA Medical Center, 3200 Vine Street, Cincinnati, OH 45220 USA
    Curr Med Chem 16:2514-30. 2009
  3. pmc Echinocandin treatment of pneumocystis pneumonia in rodent models depletes cysts leaving trophic burdens that cannot transmit the infection
    Melanie T Cushion
    College of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of America
    PLoS ONE 5:e8524. 2010
  4. pmc Transcriptome of Pneumocystis carinii during fulminate infection: carbohydrate metabolism and the concept of a compatible parasite
    Melanie T Cushion
    University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, Cincinnati, Ohio, United States of America
    PLoS ONE 2:e423. 2007
  5. pmc Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds
    Melanie T Cushion
    University of Cincinnati College of Medicine, Division of Infectious Diseases, 231 Albert Sabin Way, Cincinnati, OH 45267 0560, USA
    Antimicrob Agents Chemother 48:4209-16. 2004
  6. pmc Time between inoculations and karyotype forms of Pneumocystis carinii f. sp. Carinii influence outcome of experimental coinfections in rats
    M T Cushion
    Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0560, USA
    Infect Immun 69:97-107. 2001
  7. pmc Biofilm formation by Pneumocystis spp
    Melanie T Cushion
    University of Cincinnati College of Medicine, 231 Albert Sabin Way, ML 560, Cincinnati, OH 45267 0560, USA
    Eukaryot Cell 8:197-206. 2009
  8. pmc Effects of atovaquone and diospyrin-based drugs on the cellular ATP of Pneumocystis carinii f. sp. carinii
    M T Cushion
    Department of Internal Medicine, University of Cincinnati College of Medicine, and Veterans Affairs Medical Center, Ohio, USA
    Antimicrob Agents Chemother 44:713-9. 2000
  9. pmc In vitro and in vivo effects of quinupristin-dalfopristin against Pneumocystis carinii
    P D Walzer
    Research Service, Veterans Affairs Medical Center, Cincinnati, Ohio 45220, USA
    Antimicrob Agents Chemother 45:3234-7. 2001
  10. ncbi request reprint Anti-human immunodeficiency virus drugs are ineffective against Pneumocystis carinii in vitro and in vivo
    P D Walzer
    Research Service, Veterans Affairs Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Infect Dis 184:1355-7. 2001

Research Grants

  1. Biofilm Formation by Pneumocystis
    Melanie T Cushion; Fiscal Year: 2010
  2. PNEUMOCYSTIS GENOME PROJECT
    Melanie Cushion; Fiscal Year: 2003
  3. Eighth International Workshops on Opportunistic Protists
    Melanie Cushion; Fiscal Year: 2003
  4. PNEUMOCYSTIS GENOME PROJECT
    Melanie Cushion; Fiscal Year: 2004
  5. New Approaches for Development of PcP Therapy
    Melanie Cushion; Fiscal Year: 2006
  6. Biofilm Formation by Pneumocystis
    Melanie Cushion; Fiscal Year: 2009
  7. TRANSMISSION OF PNEUMOCYSTIS INFECTION
    Melanie Cushion; Fiscal Year: 2001
  8. TRANSMISSION OF PNEUMOCYSTIS INFECTION
    Melanie Cushion; Fiscal Year: 1993

Collaborators

Detail Information

Publications49

  1. pmc Assembly and annotation of Pneumocystis jirovecii from the human lung microbiome
    Melanie T Cushion
    University of Cincinnati, College of Medicine, Cincinnati, Ohio, USA
    MBio 4:e00224. 2013
    ..6 kb. Knowledge of this genome will open new avenues of research, including the identification of nutritional requirements for in vitro cultivation as well as the identification of new and novel drug and vaccine targets...
  2. ncbi request reprint Preclinical drug discovery for new anti-pneumocystis compounds
    Melanie T Cushion
    Research Service 151, VA Medical Center, 3200 Vine Street, Cincinnati, OH 45220 USA
    Curr Med Chem 16:2514-30. 2009
    ..Despite the significant obstacles for drug discovery, progress in identifying novel agents has been made with current systems and the promise of future new targets is expected with the annotation of the Pneumocystis genome...
  3. pmc Echinocandin treatment of pneumocystis pneumonia in rodent models depletes cysts leaving trophic burdens that cannot transmit the infection
    Melanie T Cushion
    College of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of America
    PLoS ONE 5:e8524. 2010
    ..Importantly, the echinocandins provide novel and powerful chemical tools to probe the still poorly understood bi-phasic life cycle of this genus of fungal pathogens...
  4. pmc Transcriptome of Pneumocystis carinii during fulminate infection: carbohydrate metabolism and the concept of a compatible parasite
    Melanie T Cushion
    University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, Cincinnati, Ohio, United States of America
    PLoS ONE 2:e423. 2007
    ..This study represents the first characterization of the expressed genes of a non-culturable fungal pathogen of mammals during the infective process...
  5. pmc Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds
    Melanie T Cushion
    University of Cincinnati College of Medicine, Division of Infectious Diseases, 231 Albert Sabin Way, Cincinnati, OH 45267 0560, USA
    Antimicrob Agents Chemother 48:4209-16. 2004
    ..The strategy of substitution of 1,4-piperazine-linked bisbenzamidines produced compounds with the highest level of activity observed in the ATP assay and holds great promise for the development of efficacious anti-P. carinii therapy...
  6. pmc Time between inoculations and karyotype forms of Pneumocystis carinii f. sp. Carinii influence outcome of experimental coinfections in rats
    M T Cushion
    Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0560, USA
    Infect Immun 69:97-107. 2001
    ..carinii f. sp. carinii karyotype profiles were associated with differences in biological responses. This model provides a useful method for the study of P. carinii coinfections and their transmission in humans...
  7. pmc Biofilm formation by Pneumocystis spp
    Melanie T Cushion
    University of Cincinnati College of Medicine, 231 Albert Sabin Way, ML 560, Cincinnati, OH 45267 0560, USA
    Eukaryot Cell 8:197-206. 2009
    ..Identification of this biofilm process provides a tractable in vitro system that should fundamentally advance the study of Pneumocystis...
  8. pmc Effects of atovaquone and diospyrin-based drugs on the cellular ATP of Pneumocystis carinii f. sp. carinii
    M T Cushion
    Department of Internal Medicine, University of Cincinnati College of Medicine, and Veterans Affairs Medical Center, Ohio, USA
    Antimicrob Agents Chemother 44:713-9. 2000
    ..carinii at nanomolar concentrations. These data indicate that although naphthoquinones inhibit the electron transport chain, the molecular targets in a given organism are likely to be distinct among members of this class of compounds...
  9. pmc In vitro and in vivo effects of quinupristin-dalfopristin against Pneumocystis carinii
    P D Walzer
    Research Service, Veterans Affairs Medical Center, Cincinnati, Ohio 45220, USA
    Antimicrob Agents Chemother 45:3234-7. 2001
    ..We conclude that Q-D has activity against P. carinii in vitro and in vivo...
  10. ncbi request reprint Anti-human immunodeficiency virus drugs are ineffective against Pneumocystis carinii in vitro and in vivo
    P D Walzer
    Research Service, Veterans Affairs Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Infect Dis 184:1355-7. 2001
    ..carinii in these in vitro and in vivo systems. Caution should be used when interpreting reports of the susceptibility of P. carinii to anti-HIV drugs on the basis of in vitro testing only...
  11. pmc In vitro selection and in vivo efficacy of piperazine- and alkanediamide-linked bisbenzamidines against Pneumocystis pneumonia in mice
    Melanie T Cushion
    Research Service, Veterans Affairs Medical Center, Cincinnati, OH 45220, USA
    Antimicrob Agents Chemother 50:2337-43. 2006
    ..We conclude that a number of these bisbenzamidine compounds, especially compound 100, may show promise as new anti-Pneumocystis drugs...
  12. ncbi request reprint Comparative genomics of Pneumocystis carinii with other protists: implications for life style
    Melanie T Cushion
    University of Cincinnati College of Medicine, Division of Infectious Diseases, Cincinnati, Ohio 45267 0560, USA
    J Eukaryot Microbiol 51:30-7. 2004
    ....
  13. ncbi request reprint Expression profiling of the responses of Pneumocystis carinii to drug treatment using DNA macroarrays
    Margaret S Collins
    University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    J Eukaryot Microbiol 50:605-6. 2003
  14. pmc Noninvasive method for monitoring Pneumocystis carinii pneumonia
    Michael J Linke
    Veterans Affairs Medical Center, Cincinnati, Ohio, USA
    Emerg Infect Dis 9:1613-6. 2003
    ..carinii organisms in the rats' lungs, as enumerated by microscopic methods. This report is the first of a noninvasive, antemortem method that can be used to monitor infection in a host over time...
  15. doi request reprint Stealth and opportunism: alternative lifestyles of species in the fungal genus Pneumocystis
    Melanie T Cushion
    Department of Internal Medicine, University of Cincinnati College of Medicine, Division of Infectious Diseases, Cincinnati, Ohio 45267 0560, USA
    Annu Rev Microbiol 64:431-52. 2010
    ....
  16. ncbi request reprint The genome of Pneumocystis carinii
    J R Stringer
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267 0560, USA
    FEMS Immunol Med Microbiol 22:15-26. 1998
    ..carinii genome is both practicable and of primary importance to the understanding of this organism. The small size of the P. carinii genome and its packaging into chromosomes that are resolvable by PFGE will facilitate sequence analysis...
  17. pmc Inhibitors of sterol biosynthesis and amphotericin B reduce the viability of pneumocystis carinii f. sp. carinii
    E S Kaneshiro
    Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221, USA
    Antimicrob Agents Chemother 44:1630-8. 2000
    ..carinii infections at clinical doses; however, this drug apparently binds to sterols and causes permeability changes in P. carinii membranes, since it reduced cellular ATP levels in a dose-dependent fashion...
  18. pmc A cytotoxicity assay for evaluation of candidate anti-Pneumocystis carinii agents
    M T Cushion
    Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio 45267 0560, USA
    Antimicrob Agents Chemother 41:379-84. 1997
    ..The cytotoxicity assay provides a rapid assessment of response, does not rely on replicating organisms, and should be useful for assessment of structure-function relationships...
  19. pmc Functional characterization and localization of Pneumocystis carinii lanosterol synthase
    Tiffany M Joffrion
    Department of Internal Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0560, USA
    Eukaryot Cell 9:107-15. 2010
    ..carinii. Moreover, the yeast heterologous system should be a useful tool for further analysis of the P. carinii sterol pathway...
  20. ncbi request reprint The International Workshops on Opportunistic Protists
    Edna S Kaneshiro
    Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221 0006, USA
    J Eukaryot Microbiol 53:S1-7. 2006
  21. ncbi request reprint Large-scale characterization of introns in the Pneumocystis carinii genome
    Bradley E Slaven
    University of Cincinnati College of Medicine, Cincinnati, Ohio 46267 0560, USA
    J Eukaryot Microbiol 53:S151-3. 2006
  22. ncbi request reprint Draft assembly and annotation of the Pneumocystis carinii genome
    Bradley E Slaven
    University of Cincinnati College of Medicine, Cincinnati, Ohio 46267 0560, USA
    J Eukaryot Microbiol 53:S89-91. 2006
  23. doi request reprint Sterol biosynthesis and sterol uptake in the fungal pathogen Pneumocystis carinii
    Tiffany M Joffrion
    Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA
    FEMS Microbiol Lett 311:1-9. 2010
    ..carinii sterol genes, along with biochemical analyses of the lipid content of P. carinii cellular membranes, have provided an insight into sterol biosynthesis and the sterol-scavenging mechanisms used by these fungi...
  24. pmc Early acquisition of Pneumocystis carinii in neonatal rats as evidenced by PCR and oral swabs
    Crystal R Icenhour
    Department of Infectious Disease, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0560, USA
    Eukaryot Cell 1:414-9. 2002
    ..No P. carinii amplicons were produced from 48 fetuses or their placentae taken by cesarean section. Thus, P. carinii is acquired almost immediately after birth, and placental transmission occurs rarely, if ever, in rats...
  25. pmc Gene arrays at Pneumocystis carinii telomeres
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Genetics 170:1589-600. 2005
    ..Long mononucleotide tracts were present in some MSR genes. These unstable sequences can be expected to suffer frequent frameshift mutations, providing P. carinii with another mechanism to generate antigen variation...
  26. doi request reprint Highlights and summaries of the 11th International Workshops on Opportunistic Protists
    Edna S Kaneshiro
    Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221 0006, USA
    J Eukaryot Microbiol 58:1-6. 2011
    ....
  27. ncbi request reprint Phylogenetic identification of Pneumocystis murina sp. nov., a new species in laboratory mice
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0524, USA
    Microbiology 150:1153-65. 2004
    ..murina, the results indicated that P. murina is as old as the mouse. Taken together, these data support previous recognition of multiple species in the genus and indicate that P. murina is a phylogenetic species as well...
  28. doi request reprint Sequence and structure of the linear mitochondrial genome of Pneumocystis carinii
    Thomas M Sesterhenn
    Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45220 0560, USA
    Mol Genet Genomics 283:63-72. 2010
    ..Together with the identification of terminal repeats similar to those found in other linear fungal mt genomes and the inability to join the ends by PCR, these data provide strong evidence that the mt genome of P. carinii is linear...
  29. pmc Widespread occurrence of Pneumocystis carinii in commercial rat colonies detected using targeted PCR and oral swabs
    C R Icenhour
    Department of Infectious Disease, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0560, USA
    J Clin Microbiol 39:3437-41. 2001
    ..carinii f. sp. carinii DNA in all rat lungs, illustrating widespread occurrence of Pneumocystis in commercial rat colonies. Thus, oral swab/PCR is a rapid, nonlethal, and sensitive method for the assessment of Pneumocystis exposure...
  30. ncbi request reprint Pneumocystis: unraveling the cloak of obscurity
    Melanie T Cushion
    Cincinnati VAMC and the University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0560, USA
    Trends Microbiol 12:243-9. 2004
  31. ncbi request reprint Microaerophilic conditions increase viability and affect responses of Pneumocystis carinii to drugs in vitro
    Tiffany M Joffrion
    University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0560, USA
    J Eukaryot Microbiol 53:S117-8. 2006
  32. pmc Diversity at the locus associated with transcription of a variable surface antigen of Pneumocystis carinii as an index of population structure and dynamics in infected rats
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
    Infect Immun 71:47-60. 2003
    ..By contrast, shifts in population structure occurred in rats given 10(4) P. carinii organisms, suggesting that a small fraction of these proliferated...
  33. ncbi request reprint Pneumocystis: not just pneumonia
    J Chad Peterson
    Department of Infectious Disease, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0560, USA
    Curr Opin Microbiol 8:393-8. 2005
    ..Also, the role of Pneumocystis has been investigated as a possible co-morbidity factor...
  34. pmc The ste3 pheromone receptor gene of Pneumocystis carinii is surrounded by a cluster of signal transduction genes
    A G Smulian
    Infectious Disease Division, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0560, USA
    Genetics 157:991-1002. 2001
    ..The P. carinii genome contains much of the genetic machinery necessary for pheromone responsiveness, and these data support the existence of a sexual replication cycle...
  35. ncbi request reprint Pneumocystis 2006: summary of the research presented at the ninth International Workshop on Opportunistic Protists
    Melanie T Cushion
    University of Cincinnati College of Medicine, and the Veterans Affairs Medical Center, Cincinnati, Ohio 45220, USA
    J Eukaryot Microbiol 53:S80-4. 2006
  36. ncbi request reprint Sequence of the mitochondrial genome of Pneumocystis carinii: implications for biological function and identification of potential drug targets
    Thomas M Sesterhenn
    University of Cincinnati, Cincinnati, Ohio, USA
    J Eukaryot Microbiol 53:S154-5. 2006
  37. pmc Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription
    Scott P Keely
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA
    Fungal Genet Biol 44:905-19. 2007
    ..carinii, but the block of conserved sequence in P. murina is nearly five times longer than in P. carinii, and much shorter than in P. wakefieldiae. These data indicate that the P. murina expression-site locus has a distinct structure...
  38. ncbi request reprint Generation of sequencing libraries for the Pneumocystis Genome project
    Thomas M Sesterhenn
    University of Cincinnati, Cincinnati, OH, USA
    J Eukaryot Microbiol 50:663-5. 2003
  39. pmc Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool
    Zheng Xu
    Department of Genetics, University of Georgia, Athens, Georgia 30602, USA
    Genetics 163:1299-313. 2003
    ..e., MES1) in Saccharomyces cerevisiae. Physical mapping data are consistent with the 16S, 5.8S, and 26S rDNA genes being single copy in P. carinii. No other fungus outside this genus is known to have the rDNA genes in single copy...
  40. ncbi request reprint Fine analysis of the Pneumocystis carinii f. sp. carinii genome by two-dimensional pulsed-field gel electrophoresis
    Emmanuel Cornillot
    Parasitologie Moléculaire et Cellulaire, LBP, UMR CNRS 6023, Universite Blaise Pascal, Bâtiment Biologie A, Campus Universitaire des Cezeaux, Aubiere, France
    Gene 293:87-95. 2002
    ..Hybridization data indicated that insertion/deletion events may have occurred within subtelomeric regions which carry genes encoding the major surface glycoprotein (MSG) of Pneumocystis...
  41. ncbi request reprint Novel bisbenzamidines as potential drug candidates for the treatment of Pneumocystis carinii pneumonia
    Jean Jacques Vanden Eynde
    Xavier University of Louisiana, College of Pharmacy, Division of Basic Pharmaceutical Sciences, 1 Drexel Drive, New Orleans 70125, USA
    Bioorg Med Chem Lett 14:4545-8. 2004
    ....
  42. ncbi request reprint Competitive coexistence of two Pneumocystis species
    Crystal R Icenhour
    Mayo Clinic and Foundation, Thoracic Disease Research Unit, Rochester, MN 55905, USA
    Infect Genet Evol 6:177-86. 2006
    ..To our knowledge, this is the first report to evaluate interactions of pathogenic fungal species within a mammalian host using ecological models...
  43. ncbi request reprint Anti-Pneumocystis carinii and antiplasmodial activities of primaquine-derived imidazolidin-4-ones
    Nuno Vale
    CIQUP Centro de Investigação em Química da Universidade do Porto, Departamento de Quimica, Faculdade de Ciencias, Universidade do Porto, P 4169 007 Porto, Portugal
    Bioorg Med Chem Lett 18:485-8. 2008
    ..carinii 8-aminoquinolines with increased stability and resistance to metabolic inactivation...
  44. doi request reprint Novel bisbenzimidazoles with antileishmanial effectiveness
    Annie Mayence
    Xavier University of Louisiana, College of Pharmacy, Division of Basic Pharmaceutical Sciences, 1 Drexel Drive, New Orleans, LA 70125, USA
    Bioorg Med Chem Lett 18:2658-61. 2008
    ..Among the six tested compounds two derivatives emerged as promising hits characterized by IC(50) values lower than that determined for pentamidine against L. donovani...
  45. ncbi request reprint Evidence for multiple sterol methyl transferase pathways in Pneumocystis carinii
    Wenxu Zhou
    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409 1061, USA
    Lipids 37:1177-86. 2002
    ..carinii with no counterpart in human biochemistry suggests a close taxonomic affinity with fungi and provides a basis for mechanism-based inactivation of SMT enzyme to treat Pneumocystis pneumonia...
  46. ncbi request reprint Has the name really been changed? It has for most researchers
    Melanie T Cushion
    Clin Infect Dis 41:1756-8. 2005
  47. ncbi request reprint Parallel solution-phase synthesis of conformationally restricted congeners of pentamidine and evaluation of their antiplasmodial activities
    Annie Mayence
    College of Pharmacy, Division of Basic Pharmaceutical Sciences, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, Louisiana 70125, USA
    J Med Chem 47:2700-5. 2004
    ..DNA binding affinity, as estimated by DeltaT(m), did not correlate with either antiparasite effects or cytotoxicity. Each of the active bisbenzamidines interfered with the formation of hemozoin in cell-free systems...
  48. ncbi request reprint Pneumocystis and Trypanosoma cruzi: nomenclature and typifications
    Scott A Redhead
    National Program on Environmental Health Biodiversity, Agriculture and Agri Food Canada, Central Experimental Farm, KW Neatby Building, 960 Carling Avenue, Ottawa, Ontario K1A 0C6, Canada
    J Eukaryot Microbiol 53:2-11. 2006
    ..The classification and nomenclature of Pneumocystis is reviewed and guidelines given for the publication of new species...

Research Grants25

  1. Biofilm Formation by Pneumocystis
    Melanie T Cushion; Fiscal Year: 2010
    ..We identified a biofilm system for the fungal pathogen, Pneumocystis, that will be used to understand the strategies it employs to survive in the lung and cause pneumonia. ..
  2. PNEUMOCYSTIS GENOME PROJECT
    Melanie Cushion; Fiscal Year: 2003
    ..abstract_text> ..
  3. Eighth International Workshops on Opportunistic Protists
    Melanie Cushion; Fiscal Year: 2003
    ..Mead's and Cushion's ancestors include native Americans; Marciano-Cabral is Hispanic; Kaneshiro has Pacific-Asian ancestry; Lindsay, Weiss are white. Lindsay is a physically handicapped individual. ..
  4. PNEUMOCYSTIS GENOME PROJECT
    Melanie Cushion; Fiscal Year: 2004
    ..Thus, there is but a single aim to this proposal: 1. To finish sequencing the genome of pneumocystis carinii and provide a completed assembly. ..
  5. New Approaches for Development of PcP Therapy
    Melanie Cushion; Fiscal Year: 2006
    ..Finally, they will select the most efficacious combinations of agents and evaluate their effects on gene expression again using the macroarray approach. ..
  6. Biofilm Formation by Pneumocystis
    Melanie Cushion; Fiscal Year: 2009
    ..We identified a biofilm system for the fungal pathogen, Pneumocystis, that will be used to understand the strategies it employs to survive in the lung and cause pneumonia. ..
  7. TRANSMISSION OF PNEUMOCYSTIS INFECTION
    Melanie Cushion; Fiscal Year: 2001
    ..These studies will result in a clearer understanding of the biology leading to infection with Pc and will provide a rational basis for clinical management and therapeutic intervention. ..
  8. TRANSMISSION OF PNEUMOCYSTIS INFECTION
    Melanie Cushion; Fiscal Year: 1993
    ....