Research Topics
Genomes and GenesSpecies | Joseph CuschieriSummaryAffiliation: University of Cincinnati Country: USA Publications
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Publications
Modulation of macrophage responsiveness to lipopolysaccharide by IRAK-1 manipulationJoseph Cuschieri
Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45267, USA
Shock 21:182-8. 2004..Therefore, IRAK-1 appears to be the central kinase involved in the activation of the macrophage at distant sites during septic shock but is not necessary for activation in areas of local infection...- Implications of proteasome inhibition: an enhanced macrophage phenotypeJoseph Cuschieri
Department of Surgery, University of Cincinnati, 231 Albert Sabin Way, ML 558 Cincinnati, OH 45267 0558, USA
Cell Immunol 227:140-7. 2004..Thus, this study demonstrates that cellular proteasome is critical to regulation of LPS-induced signaling within the macrophage, and inhibition of the proteasome results in a conversion to an anti-inflammatory phenotype... - Modulation of endotoxin-induced endothelial function by calcium/calmodulin-dependent protein kinaseJoseph Cuschieri
Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45267, USA
Shock 20:176-82. 2003..Therefore, CaMK inhibition of endothelial cells, characteristic of sustained increases in intracellular calcium, appears to result in a dysregulated proadhesive phenotype... - Calcium/calmodulin-dependent kinase II is required for platelet-activating factor primingJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, WA 98104, USA
Shock 23:99-106. 2005..This priming event is mediated in part by modulation of ERK 1/2, JNK/SAPK, NF-kappaB, and AP-1 activation. CaMK IV, on the other hand, is not specific for priming by PAF and appears to have a direct link in TLR4-mediated events... - Modulation of endotoxin-induced endothelial activity by microtubule depolymerizationJoseph Cuschieri
Department of Surgery, Division of Trauma Critical Care, University of Cincinnati, 231 Albert Sabin Way, ML 558, Cincinnati, OH 45267 0558, USA
J Trauma 54:104-12; discussion 112-3. 2003..The potential role of this polymerization event, however, is unknown... - Insulin regulates macrophage activation through activin AJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington, USA
Shock 29:285-90. 2008..As a result, we set out to investigate the role and means of activation of this pathway by insulin on endotoxin-mediated activation of tissue-fixed macrophages... - Platelet-activating factor priming of inflammatory cell activity requires cellular adherenceJoseph Cuschieri
Department of Surgery, University of Cincinnati, Ohio 45267-0558, USA
Surgery 132:157-66. 2002..This, therefore, would limit PAF-induced priming in vivo to foci of stimulated adherent inflammatory cells with little effect systemically on circulating cells... - Hypothermia in massive transfusion: have we been paying enough attention to it?Benjamin R Reynolds
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
J Trauma Acute Care Surg 73:486-91. 2012..We sought to characterize the importance of temperature as a risk factor for poor outcome relative to the changes in MT resuscitation that have occurred... - Slow channel calcium inhibition blocks proinflammatory gene signaling and reduces macrophage responsivenessJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington 98104, USA
J Trauma 52:434-42. 2002..This study investigates the possible intracellular mechanisms responsible for calcium antagonist protection in tissue-fixed macrophages, a central modulator of the proinflammatory phenotype... 
Body adipose content is independently associated with a higher risk of organ failure and nosocomial infection in the nonobese patient postinjuryRebecca D Edmonds
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
J Trauma 70:292-8. 2011..We sought to avoid the confounding effects attributable to obesity and determine the association of BMI and outcomes following injury in nonobese patients...- Differences in outcome between obese and nonobese patients following severe blunt trauma are not consistent with an early inflammatory genomic responseRobert D Winfield
Department of Surgery, Laboratory of Inflammation Biology and Surgical Science, University of Florida College of Medicine, Gainesville, FL, USA
Crit Care Med 38:51-8. 2010..The effect of obesity on severely injured patients, however, remains incompletely defined. We sought to unravel potential physiologic and genomic alterations induced by obesity in severely injured blunt trauma patients... - Timing of intubation and ventilator-associated pneumonia following injuryHeather L Evans
Department of Surgery, Harborview Medical Center, University of Washington, Seattle, 98104, USA
Arch Surg 145:1041-6. 2010.... - Acid sphingomyelinase is required for lipid Raft TLR4 complex formationJoseph Cuschieri
Department of Surgery, University of Washington, Harborview Medical Center, 325 Ninth Avenue, Seattle, WA 98104, USA
Surg Infect (Larchmt) 8:91-106. 2007..However, the mechanism responsible for these events remains unknown... - Obese patients show a depressed cytokine profile following severe blunt injuryRobert D Winfield
Laboratory of Inflammation Biology and Surgical Science, Department of Surgery, University of Florida College of Medicine, Gainesville, USA
Shock 37:253-6. 2012..This may confer increased susceptibility to nosocomial infection and later MOF. Further study of immune dysfunction in the postinjury obese patient should assess the possibility of early immune suppression... - PKC-zeta is essential for endotoxin-induced macrophage activationJoseph Cuschieri
Department of Surgery, University of Cincinnati, 231 Albert Sabin Way, ML 558, Cincinnati, OH 45267 0558, USA
J Surg Res 121:76-83. 2004..The aim of the current study was to further clarify the mechanism of LPS activation through the TLR4 receptor complex by examining the roles of the various isoforms of PKC... - Inflammation and the host response to injury, a large-scale collaborative project: patient-oriented research core--standard operating procedures for clinical care VII--Guidelines for antibiotic administration in severely injured patientsMichael A West
Department of Surgery, University of California, San Francisco, San Francisco, California 94110, USA
J Trauma 65:1511-9. 2008..It should also be noted that not all patients with increased temperature/WBC have an infection. Discontinuation of antibiotics is appropriate if cultures and other diagnostic studies are negative... - Ventilator-associated pneumonia: bacteremia and death after traumatic injuryGRANT E O'KEEFE
Department of Surgery, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
J Trauma Acute Care Surg 72:713-9. 2012..We tested the hypothesis that bacteremia was associated with death in trauma patients who developed VAP... 
Crystalloid to packed red blood cell transfusion ratio in the massively transfused patient: when a little goes a long wayMatthew D Neal
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
J Trauma Acute Care Surg 72:892-8. 2012..We hypothesized that an increased crystalloid:PRBC (C:PRBC) ratio would be associated with increased morbidity and poor outcome after MT...- The priming effect of C5a on monocytes is predominantly mediated by the p38 MAPK pathwayValerie Schaeffer
Department of Surgery, Harborview Medical Center, Seattle, Washington 98104, USA
Shock 27:623-30. 2007..On the contrary, in PBMCs, C5a activates the p38 cascade, and this pathway plays a major role in the C5a enhancement of LPS-induced IL-6 and TNF-alpha production... - Antiplatelet therapy is associated with decreased transfusion-associated risk of lung dysfunction, multiple organ failure, and mortality in trauma patientsJeffrey N Harr
Department of Surgery, University of Colorado Denver, Aurora, CO 80045, USA
Crit Care Med 41:399-404. 2013..To determine whether prehospital antiplatelet therapy was associated with reduced incidence of acute lung dysfunction, multiple organ failure, and mortality in blunt trauma patients... - Oxidant alterations in CD16 expression are cytoskeletal inducedJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington, USA
Shock 32:572-7. 2009.... - Early elevation in random plasma IL-6 after severe injury is associated with development of organ failureJoseph Cuschieri
Department of Surgery, Harborview Medical Center, University of Washington, Seattle, Washington 98104, USA
Shock 34:346-51. 2010..This measurement may be useful as a biomarker for prognosis and serve to identify patients at higher risk of adverse outcome that would benefit from novel therapeutic interventions... - Fresh frozen plasma is independently associated with a higher risk of multiple organ failure and acute respiratory distress syndromeGregory A Watson
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
J Trauma 67:221-7; discussion 228-30. 2009..We hypothesized that plasma-rich transfusion components would be independently associated with a lower risk of mortality but result in a greater risk of morbid complications... - Platelet-activating factor acetylhydrolase inhibits alveolar macrophage activation in vivoEileen M Bulger
Department of Surgery, University of Washington, Harborview Medical Center, 325 Ninth Avenue, Seattle, WA 98104, USA
Shock 20:17-22. 2003..This effect of PAF-AH may be mediated by the inhibition of intracellular signaling via inhibition of ERK kinase and NF-kappaB activation... - Hypertonic preconditioning inhibits macrophage responsiveness to endotoxinJoseph Cuschieri
Department of Surgery, University of Washington, 325 Ninth Avenue, Seattle, WA 98104, USA
J Immunol 168:1389-96. 2002..e., urea) had no significant effect. Hypertonic saline or mannitol resuscitation, therefore, may help protect against multiple-organ dysfunction syndrome as a result of this reduced proinflammatory responsiveness... - Implications of lipid raft disintegration: enhanced anti-inflammatory macrophage phenotypeJoseph Cuschieri
Department of Surgery, University of Cincinnati, Ohio USA
Surgery 136:169-75. 2004..Therefore, modulation of lipid raft cholesterol content may represent a potential mechanism for regulation of macrophage phenotypic differentiation... - Male gender is associated with excessive IL-6 expression following severe injuryJason L Sperry
Division of Trauma and General Surgery, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
J Trauma 64:572-8; discussion 578-9. 2008.... - Early supplemental parenteral nutrition is associated with increased infectious complications in critically ill trauma patientsMatthew J Sena
Department of Surgery, University of California Davis Medical Center, Sacramento, CA, USA
J Am Coll Surg 207:459-67. 2008..The purpose of this study is to determine whether early administration of parenteral nutrition is associated with an increased risk for infection after severe injury... - Characterization of acute coagulopathy and sexual dimorphism after injury: females and coagulopathy just do not mixJoshua B Brown
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
J Trauma Acute Care Surg 73:1395-400; discussion 1400. 2012..It remains unclear whether presence of ATC alters sex-based outcomes after injury. This study's objective was to characterize the sex dimorphism after severe injury in the presence and absence of ATC... - A genomic storm in critically injured humansWenzhong Xiao
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
J Exp Med 208:2581-90. 2011..Based on these transcriptional data, we propose a new paradigm for the human immunological response to severe injury... - Inflammation and the host response to injury a large-scale collaborative project: patient-oriented research core standard operating procedure for clinical care X. Guidelines for venous thromboembolism prophylaxis in the trauma patientJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington, USA
J Trauma 65:944-50. 2008 - Early blood product and crystalloid volume resuscitation: risk association with multiple organ dysfunction after severe blunt traumatic injuryScott C Brakenridge
Department of Surgery, Division of Burn Trauma Critical Care, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9158, USA
J Trauma 71:299-305. 2011..We sought to further delineate the relevance of the major individual components of early volume resuscitation to onset of MOD after severe blunt traumatic injury... - Vitamin E inhibits endotoxin-mediated transport of phosphatases to lipid raftsJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington, USA
Shock 27:19-24. 2007..Antioxidant exposure in the form of vitamin E seems to attenuate endotoxin-mediated SHIP activation resulting in increased AKT activity, and attenuated MAPK activation and TNF-alpha production... - Oxidative-induced calcium mobilization is dependent on annexin VI release from lipid raftsJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, USA
Surgery 138:158-64. 2005.... - Changes in massive transfusion over time: an early shift in the right direction?Benjamin C Kautza
Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
J Trauma Acute Care Surg 72:106-11. 2012..We sought to characterize changes in resuscitation which have occurred over time in a cohort severely injured patients requiring MT... - Phosphatidylcholine-specific phospholipase C (PC-PLC) is required for LPS-mediated macrophage activation through CD14Joseph Cuschieri
Department of Surgery, University of Washington, Seattle, WA, USA
J Leukoc Biol 80:407-14. 2006..This study demonstrates that TLR4 assembly and activation following LPS exposure require the production of ceramide by PC-PLC, which appears to be CD14-dependent... - Pre-hospital intubation factors and pneumonia in trauma patientsHeather L Evans
Department of Surgery, Harborview Medical Center, University of Washington, Seattle, Washington 98104, USA
Surg Infect (Larchmt) 12:339-44. 2011..A subset of PH intubations with continuous quality assessment was re-examined to identify the intubation factors associated with VAP... - Clinical microfluidics for neutrophil genomics and proteomicsKenneth T Kotz
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Nat Med 16:1042-7. 2010.... - Oxidative stress, lipid rafts, and macrophage reprogrammingJoseph Cuschieri
University of Washington, Department of Surgery, Seattle, Washington 98104, USA
Antioxid Redox Signal 9:1485-97. 2007..Within this review, the complexity of oxidant-induced reprogramming within the tissue fixed macrophage as currently understood is explained... - Emergency department ventilation effects outcome in severe traumatic brain injuryKeir J Warner
Department of Surgery, University of Washington, Harborview Medical Center, Seattle, Washington, USA
J Trauma 64:341-7. 2008..We sought to determine whether achieving ventilation into a target range would translate into better outcomes in patients with TBI... - Increased neutrophil adenosine a3 receptor expression is associated with hemorrhagic shock and injury severity in trauma patientsEileen M Bulger
Department of Surgery, University of Washington, Harborview Medical Center, 325 9th Ave, Seattle, WA 98104, USA
Shock 36:435-9. 2011..Hypertonic saline increases A3 expression of PMNs from healthy volunteers and less severely injured patients... - Effect of chlorhexidine whole-body bathing on hospital-acquired infections among trauma patientsHeather L Evans
Department of Surgery, Harborview Medical Center, 325 9th Ave, Seattle, WA 98104, USA
Arch Surg 145:240-6. 2010..To demonstrate whether daily bathing with cloths impregnated with 2% chlorhexidine gluconate will decrease colonization of resistant bacteria and reduce the rates of health care-associated infections in critically injured patients... - Effect of immediate enteral feeding on trauma patients with an open abdomen: protection from nosocomial infectionsSharmila Dissanaike
Department of Surgery, University of Washington, Seattle, WA 98104, USA
J Am Coll Surg 207:690-7. 2008..Despite increased use, uncertainty exists about the safety and timing of enteral nutrition. The purpose of this study was to determine the safety and effect of immediate enteral nutrition... - Hypertonic resuscitation modulates the inflammatory response in patients with traumatic hemorrhagic shockEileen M Bulger
Department of Surgery, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
Ann Surg 245:635-41. 2007..To determine the effect of resuscitation with hypertonic saline/dextran (HSD) on the innate immune response after injury... - Single-dose etomidate for rapid sequence intubation may impact outcome after severe injuryKeir J Warner
Department of Surgery, University of Washington, Harborview Medical Center, Seattle, Washington 98104, USA
J Trauma 67:45-50. 2009..We hypothesized that etomidate may alter the occurrence of acute respiratory distress syndrome (ARDS) in injury victims... - Assessment of functional limitation after necrotizing soft tissue infectionTam N Pham
Department of Surgery, Harborview Medical Center, University of Washington, Seattle, Washington, USA
J Burn Care Res 30:301-6. 2009..Development of validated functional assessment tools and accurate longitudinal follow-up are necessary to measure the functional impact of NSTI... - Traditional resuscitative practices fail to resolve metabolic acidosis in morbidly obese patients after severe blunt traumaRobert D Winfield
Laboratory of Inflammation Biology and Surgical Science, Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, USA
J Trauma 68:317-30. 2010..Evaluation of differences in resuscitative practices altered by body mass index (BMI) might provide an explanation for the increased risk of MOF seen in these high-risk patients... - Sew it up! A Western Trauma Association multi-institutional study of enteric injury management in the postinjury open abdomenClay Cothren Burlew
Department of Surgery, Denver Health Medical Center, University of Colorado, Denver, Colorado 80204, USA
J Trauma 70:273-7. 2011..The purpose of this study was to determine outcomes on the basis of management of enteric injuries in patients relegated to the postinjury open abdomen... - Inflammation and the host response to injury, a large-scale collaborative project: patient-oriented research core-standard operating procedures for clinical care: VI. Blood glucose control in the critically ill trauma patientBrian G Harbrecht
Department of Surgery, University of Louisville, Louisville, Kentucky, USA
J Trauma 63:703-8. 2007 - Mitogen-activated protein kinase (MAPK)Joseph Cuschieri
Department of Surgery, University of Washington, Seattle, WA, USA
Crit Care Med 33:S417-9. 2005 - NADPH oxidase activation in fibronectin adherent human neutrophils: A potential role for beta1 integrin ligationKonstantin Umanskiy
Division of Trauma and Critical Care, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA
Surgery 134:378-83. 2003..This appears to be mediated by beta(1) integrins. We demonstrate formation of focal adhesions containing Pyk2/FAK, paxillin, and Lyn, and translocation of p47(phox)... - Negative appendectomy and imaging accuracy in the Washington State Surgical Care and Outcomes Assessment ProgramJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, WA, USA
Ann Surg 248:557-63. 2008..The Surgical Care and Outcomes Assessment Program (SCOAP) gathers chart-abstracted process of care data (such as CT/US accuracy) for general surgical procedures (including appendectomy) at most Washington State hospitals... - Surgical management of acute necrotizing lung infectionsBeth Ann Reimel
Department of Surgery, Harborview Medical Center, Seattle, USA
Can Respir J 13:369-73. 2006..Ventilated patients have a worse prognosis but can still be candidates for resection. Patients who are hemodynamically unstable appear to have better outcomes if they can be stabilized before resection... - The impact of prehospital ventilation on outcome after severe traumatic brain injuryKeir J Warner
Department of Surgery, University of Washington, Harborview Medical Center, Seattle, Washington 98104, USA
J Trauma 62:1330-6; discussion 1336-8. 2007..We sought to determine the incidence of hyperventilation among a cohort of trauma patients undergoing prehospital intubation and the impact of ventilation on outcome after severe TBI... - Necrotizing soft tissue infectionJoseph Cuschieri
Department of Surgery, University of Washington, Seattle, Washington, USA
Surg Infect (Larchmt) 9:559-62. 2008..Necrotizing soft tissue infections (NSTIs) continue to pose major problems in diagnosis and management... - Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activationJoseph Cuschieri
University of Washington, Harborview Medical Center, 325 9th Avenue, Box 359796, Seattle, WA 98104, USA
J Leukoc Biol 80:1289-97. 2006..Thus, endotoxin tolerance appears to be induced through attenuated TLR4 formation following LPS stimulation. This complex formation appears to be PKC-dependent, and restoration of PKC activity reverses tolerance...