J Cuppoletti

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. pmc Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    Cell Biochem Biophys 66:53-63. 2013
  2. doi request reprint Unoprostone isopropyl and metabolite M1 activate BK channels and prevent ET-1-induced [Ca²⁺]i increases in human trabecular meshwork and smooth muscle
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio 45267 0576, USA
    Invest Ophthalmol Vis Sci 53:5178-89. 2012
  3. pmc Contrasting effects of linaclotide and lubiprostone on restitution of epithelial cell barrier properties and cellular homeostasis after exposure to cell stressors
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    BMC Pharmacol 12:3. 2012
  4. doi request reprint Effects of lubiprostone on human uterine smooth muscle cells
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267 0576, USA
    Prostaglandins Other Lipid Mediat 86:56-60. 2008
  5. ncbi request reprint Cellular and molecular effects of unoprostone as a BK channel activator
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, PO Box 670576, Cincinnati, OH 45267 0576, USA
    Biochim Biophys Acta 1768:1083-92. 2007
  6. ncbi request reprint SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents
    John Cuppoletti
    Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Cell Physiol 287:C1173-83. 2004
  7. ncbi request reprint Sites of protein kinase A activation of the human ClC-2 Cl(-) channel
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0576, USA
    J Biol Chem 279:21849-56. 2004
  8. ncbi request reprint ClC-2 Cl- channels in human lung epithelia: activation by arachidonic acid, amidation, and acid-activated omeprazole
    J Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Cell Physiol 281:C46-54. 2001
  9. ncbi request reprint Activation of human CIC-2 Cl- channels: implications for cystic fibrosis
    J Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267 0576, USA
    Clin Exp Pharmacol Physiol 27:896-900. 2000
  10. ncbi request reprint Cloning, functional expression, and characterization of a PKA-activated gastric Cl- channel
    D H Malinowska
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267
    Am J Physiol 268:C191-200. 1995

Research Grants

  1. REGULATED CLC CL CHANNELS AND CL SECRETION AND CF
    John Cuppoletti; Fiscal Year: 2000
  2. Regulated CIC CI Channels in CI Secretion in CF
    John Cuppoletti; Fiscal Year: 2003
  3. CHLORIDE CHANNELS AND GASTRIC ACID SECRETION
    John Cuppoletti; Fiscal Year: 2003
  4. CHLORIDE CHANNELS AND GASTRIC ACID SECRETION
    John Cuppoletti; Fiscal Year: 1993
  5. Role of Regulated CIC CI Channels in CI Secretion in CF
    John Cuppoletti; Fiscal Year: 2006

Collaborators

Detail Information

Publications17

  1. pmc Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    Cell Biochem Biophys 66:53-63. 2013
    ....
  2. doi request reprint Unoprostone isopropyl and metabolite M1 activate BK channels and prevent ET-1-induced [Ca²⁺]i increases in human trabecular meshwork and smooth muscle
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio 45267 0576, USA
    Invest Ophthalmol Vis Sci 53:5178-89. 2012
    ..Effects on recombinant human prostaglandin (PG) receptors were determined...
  3. pmc Contrasting effects of linaclotide and lubiprostone on restitution of epithelial cell barrier properties and cellular homeostasis after exposure to cell stressors
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    BMC Pharmacol 12:3. 2012
    ..Comparison with effects of lubiprostone, known to promote repair of barrier function was carried out...
  4. doi request reprint Effects of lubiprostone on human uterine smooth muscle cells
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267 0576, USA
    Prostaglandins Other Lipid Mediat 86:56-60. 2008
    ..PGE(2) and PGE(1) both caused SC-51322-sensitive membrane depolarization and increased [cAMP](i). Lubiprostone has fundamentally different cellular effects from prostaglandins that are not mediated by EP receptors...
  5. ncbi request reprint Cellular and molecular effects of unoprostone as a BK channel activator
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, PO Box 670576, Cincinnati, OH 45267 0576, USA
    Biochim Biophys Acta 1768:1083-92. 2007
    ..These findings show that unoprostone has a distinctly different mechanism of action from latanoprost and PGF(2alpha). Whether unoprostone affects the BK channel directly or an unidentified signaling mechanism has not been determined...
  6. ncbi request reprint SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents
    John Cuppoletti
    Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Cell Physiol 287:C1173-83. 2004
    ..Together, these studies demonstrate that SPI-0211 is a potent activator of ClC-2 Cl- channels and suggest a physiologically relevant role for ClC-2 Cl- channels in intestinal Cl- transport after SPI-0211 administration...
  7. ncbi request reprint Sites of protein kinase A activation of the human ClC-2 Cl(-) channel
    John Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0576, USA
    J Biol Chem 279:21849-56. 2004
    ..Either RRAT655 or RGET691 was sufficient for activation at pH(o) 7.4. RGET, but not RRAT, was sufficient for activation at pH(o) 6.0. However, in the RGET691(D) mutant, there was PKA activation at pH(o) 6.0...
  8. ncbi request reprint ClC-2 Cl- channels in human lung epithelia: activation by arachidonic acid, amidation, and acid-activated omeprazole
    J Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Cell Physiol 281:C46-54. 2001
    ..Similar results were obtained with buccal cells from healthy individuals and cystic fibrosis patients. The ClC-2 Cl- channel is thus a potential target for therapy in cystic fibrosis...
  9. ncbi request reprint Activation of human CIC-2 Cl- channels: implications for cystic fibrosis
    J Cuppoletti
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267 0576, USA
    Clin Exp Pharmacol Physiol 27:896-900. 2000
    ..3. Activators of CIC-2 Cl- channels that have therapeutic potential include amidation and omeprazole and, perhaps, effectors of arachidonic acid metabolism...
  10. ncbi request reprint Cloning, functional expression, and characterization of a PKA-activated gastric Cl- channel
    D H Malinowska
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267
    Am J Physiol 268:C191-200. 1995
    ..The electrophysiological and regulatory properties of the cloned and the native channel were similar. The cloned protein may be the Cl- channel involved in gastric HCl secretion...
  11. ncbi request reprint Localization of ClC-2 Cl- channels in rabbit gastric mucosa
    A M Sherry
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH 43267-0576, USA
    Am J Physiol Cell Physiol 280:C1599-606. 2001
    ....
  12. ncbi request reprint Gastric parietal cell secretory membrane contains PKA- and acid-activated Kir2.1 K+ channels
    Danuta H Malinowska
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0576, USA
    Am J Physiol Cell Physiol 286:C495-506. 2004
    ..Cation selectivity was K+ = Rb+ >> Na+ = Cs+ = Li+ = NMDG+. These findings strongly suggest that the Kir2.1 K+ channel may be involved in regulated gastric acid secretion at the parietal cell secretory membrane...
  13. ncbi request reprint Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targets
    Daniel J Hassett
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0524, USA
    Adv Drug Deliv Rev 54:1425-43. 2002
    ..aeruginosa for more effective treatment of chronic CF lung infections...
  14. ncbi request reprint Porous membranes for reconstitution of ion channels
    M A Dhoke
    Department of Materials Sciences and Engineering, University of Cincinnati, Cincinnati, OH 45221 0012, USA
    Biochim Biophys Acta 1716:117-25. 2005
    ....
  15. ncbi request reprint An immunoreactive 8-azido ATP-labeled protein common to the lysosomal and chromaffin granule membrane
    J Cuppoletti
    Department of Physiology and Biophysics, University of Cincinnati College of Medicine, OH 45267 0576
    Biochim Biophys Acta 946:33-9. 1988
    ..This raises the possibility that the 70 kDa 8-azido-ATP-reactive, immunologically similar proteins may play a similar role in pump function such as ATP binding and/or hydrolysis in these organelles...
  16. ncbi request reprint Solid support membranes for ion channel arrays and sensors: application to rapid screening of pharmacological compounds
    Nobunaka Matsuno
    Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221 0172, USA
    Biochim Biophys Acta 1665:184-90. 2004
    ..5 in biological membranes. SSM-mounted ion channels were stable enough to be washed with buffer solution and reused many times, allowing solution exchange essential for pharmacological drug screening...
  17. ncbi request reprint Bryostatin-1 attenuates TNF-induced epithelial barrier dysfunction: role of novel PKC isozymes
    James Yoo
    Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Am J Physiol Gastrointest Liver Physiol 284:G703-12. 2003
    ..TNF-induced barrier dysfunction occurs independently of PKC, but selective modulation of novel PKC isozymes may regulate TNF-R1 signaling...

Research Grants21

  1. REGULATED CLC CL CHANNELS AND CL SECRETION AND CF
    John Cuppoletti; Fiscal Year: 2000
    ..Mild chemical procedures for channel activation using amidation catalysed by water soluble carbodiimides will be investigated as a novel approach to development of pharmaceuticals for treatment of cystic fibrosis patients. ..
  2. Regulated CIC CI Channels in CI Secretion in CF
    John Cuppoletti; Fiscal Year: 2003
    ..2 K channel in CF. This will also be studied in terms of CI transport, Na transport, CLCA2 function, inflammation and bacterial susceptibility. ..
  3. CHLORIDE CHANNELS AND GASTRIC ACID SECRETION
    John Cuppoletti; Fiscal Year: 2003
    ..These studies strive to address the fundamental questions regarding the molecules and mechanisms involved in regulated HCl secretion. ..
  4. CHLORIDE CHANNELS AND GASTRIC ACID SECRETION
    John Cuppoletti; Fiscal Year: 1993
    ..Preliminary results of inhibitor and pH sensitivity suggest that the recorded single Cl- channels may participate in HC1 accumulation...
  5. Role of Regulated CIC CI Channels in CI Secretion in CF
    John Cuppoletti; Fiscal Year: 2006
    ..2 K channel in CF. This will also be studied in terms of CI transport, Na transport, CLCA2 function, inflammation and bacterial susceptibility. ..