M R Culbertson

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc mRNAs encoding telomerase components and regulators are controlled by UPF genes in Saccharomyces cerevisiae
    Jeffrey N Dahlseid
    Department of Chemistry, St Olaf College, Northfield, Minnesota 55057, USA
    Eukaryot Cell 2:134-42. 2003
  2. ncbi request reprint Looking at mRNA decay pathways through the window of molecular evolution
    Michael R Culbertson
    Robert M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, Wisconsin 53706, USA
    Curr Opin Genet Dev 13:207-14. 2003
  3. ncbi request reprint RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
  4. pmc Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis
    L C Robinson
    Laboratory of Molecular Biology, Robert M Bock Laboratories, University of Wisconsin, Madison 53706
    Mol Cell Biol 13:2870-81. 1993
  5. ncbi request reprint Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins
    D Ursic
    Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
    Mol Gen Genet 249:571-84. 1995
  6. pmc The yeast SEN1 gene is required for the processing of diverse RNA classes
    D Ursic
    Laboratories of Molecular Biology and Genetics, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Nucleic Acids Res 25:4778-85. 1997
  7. pmc Yeast frameshift suppressor mutations in the genes coding for transcription factor Mbf1p and ribosomal protein S3: evidence for autoregulation of S3 synthesis
    J L Hendrick
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 157:1141-58. 2001
  8. pmc The putative nucleic acid helicase Sen1p is required for formation and stability of termini and for maximal rates of synthesis and levels of accumulation of small nucleolar RNAs in Saccharomyces cerevisiae
    T P Rasmussen
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Biol 18:6885-96. 1998
  9. pmc The essential yeast Tcp1 protein affects actin and microtubules
    D Ursic
    Laboratories of Molecular Biology and Genetics, University of Wisconsin, Madison 53706
    Mol Biol Cell 5:1065-80. 1994
  10. pmc The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivo
    D J Demarini
    Laboratory of Genetics and Molecular Biology, University of Wisconsin, Madison 53706, USA
    Mol Cell Biol 15:6311-21. 1995

Collaborators

  • D J Demarini
  • L C Robinson
  • P G Wilson
  • C Kung
  • S Swaminathan
  • M Hochstrasser
  • Janet M Wood
  • ROY LONG
  • D Ursic
  • Jonathan S Finkel
  • Wei Zheng
  • Qiaoning Guan
  • Amanda S Ford
  • Jeffrey N Dahlseid
  • Renee L Shirley
  • R L Shirley
  • J N Dahlseid
  • J L Hendrick
  • Karen Chinchilla
  • Sharon M Landers
  • Eric Neeno-Eckwall
  • Kam Wah Tsui
  • Shijie Tang
  • Robert A Zinkel
  • Xiaosong Liu
  • Brian S Yandell
  • T P Rasmussen
  • Amanda Ford
  • Michelle Desruisseaux
  • Michael J Lelivelt
  • Shinichiro Enomoto
  • Judith Berman
  • Mark McClellan
  • Jodi Lew-Smith
  • Neal Lue
  • M Rachel Richards
  • Markus Albertini
  • M G Sandbaken
  • I I Edelman
  • J Puziss
  • P Hieter
  • L R Schenkman
  • A L Atkin
  • M J Lelivelt
  • D S Conklin
  • P Leeds
  • B S Lee
  • J A McMaster

Detail Information

Publications27

  1. pmc mRNAs encoding telomerase components and regulators are controlled by UPF genes in Saccharomyces cerevisiae
    Jeffrey N Dahlseid
    Department of Chemistry, St Olaf College, Northfield, Minnesota 55057, USA
    Eukaryot Cell 2:134-42. 2003
    ..Together, these results suggest that NMD maintains the balance of gene products that control telomere length and telomeric silencing primarily by maintaining appropriate levels of STN1, TEN1, and EST2 mRNA...
  2. ncbi request reprint Looking at mRNA decay pathways through the window of molecular evolution
    Michael R Culbertson
    Robert M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, Wisconsin 53706, USA
    Curr Opin Genet Dev 13:207-14. 2003
    ..Depending on the organism, three to seven genes are required for NMD. Besides RNA surveillance, the genes required for NMD serve a second purpose by controlling the overall abundance of a substantial fraction of the transcriptome...
  3. ncbi request reprint RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  4. pmc Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis
    L C Robinson
    Laboratory of Molecular Biology, Robert M Bock Laboratories, University of Wisconsin, Madison 53706
    Mol Cell Biol 13:2870-81. 1993
    ..Further, a genetic relationship between the yck2ts allele and deletion of CDC55 indicates that the function of Yck phosphorylation may be related to that of protein phosphatase 2A activity...
  5. ncbi request reprint Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins
    D Ursic
    Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
    Mol Gen Genet 249:571-84. 1995
    ..Possible mechanisms are discussed for several related nuclear functions of Sen1p, including tRNA splicing and the maintenance of a normal crescent-shaped nucleolus...
  6. pmc The yeast SEN1 gene is required for the processing of diverse RNA classes
    D Ursic
    Laboratories of Molecular Biology and Genetics, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Nucleic Acids Res 25:4778-85. 1997
    ..We propose that Sen1 is an RNA helicase acting on a wide range of RNA classes. Its effects on the targeted RNAs in turn enable ribonuclease activity...
  7. pmc Yeast frameshift suppressor mutations in the genes coding for transcription factor Mbf1p and ribosomal protein S3: evidence for autoregulation of S3 synthesis
    J L Hendrick
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 157:1141-58. 2001
    ..A change in mRNA decay rate may be the primary mechanism for regulation...
  8. pmc The putative nucleic acid helicase Sen1p is required for formation and stability of termini and for maximal rates of synthesis and levels of accumulation of small nucleolar RNAs in Saccharomyces cerevisiae
    T P Rasmussen
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Biol 18:6885-96. 1998
    ....
  9. pmc The essential yeast Tcp1 protein affects actin and microtubules
    D Ursic
    Laboratories of Molecular Biology and Genetics, University of Wisconsin, Madison 53706
    Mol Biol Cell 5:1065-80. 1994
    ..Based on our results, we propose that Tcp1p is required for normal development and function of actin and microtubules either through direct or indirect interaction with the major cytoskeletal components...
  10. pmc The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivo
    D J Demarini
    Laboratory of Genetics and Molecular Biology, University of Wisconsin, Madison 53706, USA
    Mol Cell Biol 15:6311-21. 1995
    ....
  11. ncbi request reprint A factor required for nonsense-mediated mRNA decay in yeast is exported from the nucleus to the cytoplasm by a nuclear export signal sequence
    R L Shirley
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, WI, USA
    J Cell Sci 111:3129-43. 1998
    ..These results suggest that NES-A is a functional nuclear export signal. Combined mutations in NES-A may cause multiple defects in protein function leading to an Nmd- phenotype even when export is restored...
  12. pmc Accumulation of mRNA coding for the ctf13p kinetochore subunit of Saccharomyces cerevisiae depends on the same factors that promote rapid decay of nonsense mRNAs
    J N Dahlseid
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 150:1019-35. 1998
    ..We propose that UPF-mediated decay modulates the mRNA level of one or more factors involved in CTF13 mRNA expression...
  13. pmc SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiae
    D J Demarini
    Laboratory of Genetics, University of Wisconsin, Madison 53706
    Mol Cell Biol 12:2154-64. 1992
    ..The mutation that creates the temperature-sensitive sen1-1 allele is located within this 500-amino-acid region, and it causes a substitution for an amino acid that is conserved in all three proteins...
  14. pmc Gene products that promote mRNA turnover in Saccharomyces cerevisiae
    P Leeds
    Laboratory of Genetics, University of Wisconsin, Madison 53706
    Mol Cell Biol 12:2165-77. 1992
    ..On the basis of these results, we suggest that the products of at least two genes, UPF1 and UPF3, are responsible for the accelerated rate of mRNA decay associated with premature translational termination...
  15. pmc The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes
    D Ursic
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
    Mol Cell Biol 11:2629-40. 1991
    ..We also observed that strains carrying tcp1-1 were hypersensitive to antimitotic compounds. Taken together, these observations imply that the TCP1 protein affects microtubule-mediated processes...
  16. pmc Yeast casein kinase I homologues: an essential gene pair
    L C Robinson
    University of Wisconsin, Madison 53706
    Proc Natl Acad Sci U S A 89:28-32. 1992
    ..Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues...
  17. ncbi request reprint SUF12 suppressor protein of yeast. A fusion protein related to the EF-1 family of elongation factors
    P G Wilson
    Laboratory of Genetics, University of Wisconsin, Madison 53706
    J Mol Biol 199:559-73. 1988
    ..We propose a model of suf12-mediated frameshift and nonsense suppression that is based on a primary defect in the normal process of codon recognition...
  18. pmc Navigating without a road map
    Michael R Culbertson
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 177:1-7. 2007
  19. pmc COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae
    D S Conklin
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
    Mol Cell Biol 12:3678-88. 1992
    ..The increased tolerance of strains containing multiple copies of the COT1 gene is probably due to increased compartmentalization or sequestration of the ion within mitochondria...
  20. pmc Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiae
    Jonathan S Finkel
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 184:107-18. 2010
    ..The results suggest that the Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression...
  21. pmc Nuclear import of Upf3p is mediated by importin-alpha/-beta and export to the cytoplasm is required for a functional nonsense-mediated mRNA decay pathway in yeast
    Renee L Shirley
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 161:1465-82. 2002
    ..To explain these results, we suggest that the mutations in NES-A that impair nuclear export cause additional defects in the function of Upf3p that are not rectified by restoration of export alone...
  22. pmc Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing
    Doris Ursic
    Laboratories of Molecular Biology and Genetics, R M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Nucleic Acids Res 32:2441-52. 2004
    ..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways...
  23. pmc Ebs1p, a negative regulator of gene expression controlled by the Upf proteins in the yeast Saccharomyces cerevisiae
    Amanda S Ford
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Eukaryot Cell 5:301-12. 2006
    ..Finally, EBS1 transcript levels are under the control of nonsense-mediated mRNA decay (NMD), providing the first example of an NMD-sensitive transcript whose protein product influences a step in gene expression required for NMD...
  24. pmc Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae
    Wei Zheng
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 180:1391-405. 2008
    ..Overall, our results suggest a model in which ash1 mRNAs are insensitive to NMD while translation is repressed during transport but become sensitive once repression is relieved...
  25. ncbi request reprint Detecting phosphorylation-dependent interactions with the C-terminal domain of RNA polymerase II subunit Rpb1p using a yeast two-hybrid assay
    Doris Ursic
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    RNA Biol 5:1-4. 2008
    ..Interactions between the CTD and three known CTD-binding proteins were analyzed. The results suggest that the modified two-hybrid system accurately assays CTD-binding and provides a new and convenient assay for CTD-binding proteins...
  26. pmc Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast
    Qiaoning Guan
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Genet 2:e203. 2006
    ..Furthermore, the methods for analyzing decay rates on a global scale offer a blueprint for new ways to study mRNA decay pathways in any organism where cultured cell lines are available...
  27. pmc Transcript selection and the recruitment of mRNA decay factors for NMD in Saccharomyces cerevisiae
    Michael R Culbertson
    RNA 11:1333-9. 2005
    ..of NMD to simultaneously reduce or eliminate the synthesis of truncated proteins produced by nonsense transcripts while down-regulating but not completely eliminating functional proteins produced from error-free NMD-sensitive transcripts..