Research Topics
Genomes and Genes | M R CulbertsonSummaryAffiliation: University of Wisconsin Country: USA Publications
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Publications
mRNAs encoding telomerase components and regulators are controlled by UPF genes in Saccharomyces cerevisiaeJeffrey N Dahlseid
Department of Chemistry, St Olaf College, Northfield, Minnesota 55057, USA
Eukaryot Cell 2:134-42. 2003..Together, these results suggest that NMD maintains the balance of gene products that control telomere length and telomeric silencing primarily by maintaining appropriate levels of STN1, TEN1, and EST2 mRNA...
Looking at mRNA decay pathways through the window of molecular evolutionMichael R Culbertson
Robert M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, Wisconsin 53706, USA
Curr Opin Genet Dev 13:207-14. 2003..Depending on the organism, three to seven genes are required for NMD. Besides RNA surveillance, the genes required for NMD serve a second purpose by controlling the overall abundance of a substantial fraction of the transcriptome...- RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancerM R Culbertson
Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
Trends Genet 15:74-80. 1999..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD... - Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesisL C Robinson
Laboratory of Molecular Biology, Robert M Bock Laboratories, University of Wisconsin, Madison 53706
Mol Cell Biol 13:2870-81. 1993..Further, a genetic relationship between the yck2ts allele and deletion of CDC55 indicates that the function of Yck phosphorylation may be related to that of protein phosphatase 2A activity... - Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteinsD Ursic
Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
Mol Gen Genet 249:571-84. 1995..Possible mechanisms are discussed for several related nuclear functions of Sen1p, including tRNA splicing and the maintenance of a normal crescent-shaped nucleolus... - The yeast SEN1 gene is required for the processing of diverse RNA classesD Ursic
Laboratories of Molecular Biology and Genetics, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
Nucleic Acids Res 25:4778-85. 1997..We propose that Sen1 is an RNA helicase acting on a wide range of RNA classes. Its effects on the targeted RNAs in turn enable ribonuclease activity... - Yeast frameshift suppressor mutations in the genes coding for transcription factor Mbf1p and ribosomal protein S3: evidence for autoregulation of S3 synthesisJ L Hendrick
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 157:1141-58. 2001..A change in mRNA decay rate may be the primary mechanism for regulation... - The putative nucleic acid helicase Sen1p is required for formation and stability of termini and for maximal rates of synthesis and levels of accumulation of small nucleolar RNAs in Saccharomyces cerevisiaeT P Rasmussen
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Mol Cell Biol 18:6885-96. 1998.... - The essential yeast Tcp1 protein affects actin and microtubulesD Ursic
Laboratories of Molecular Biology and Genetics, University of Wisconsin, Madison 53706
Mol Biol Cell 5:1065-80. 1994..Based on our results, we propose that Tcp1p is required for normal development and function of actin and microtubules either through direct or indirect interaction with the major cytoskeletal components... - The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivoD J Demarini
Laboratory of Genetics and Molecular Biology, University of Wisconsin, Madison 53706, USA
Mol Cell Biol 15:6311-21. 1995.... - A factor required for nonsense-mediated mRNA decay in yeast is exported from the nucleus to the cytoplasm by a nuclear export signal sequenceR L Shirley
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, WI, USA
J Cell Sci 111:3129-43. 1998..These results suggest that NES-A is a functional nuclear export signal. Combined mutations in NES-A may cause multiple defects in protein function leading to an Nmd- phenotype even when export is restored... - Accumulation of mRNA coding for the ctf13p kinetochore subunit of Saccharomyces cerevisiae depends on the same factors that promote rapid decay of nonsense mRNAsJ N Dahlseid
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 150:1019-35. 1998..We propose that UPF-mediated decay modulates the mRNA level of one or more factors involved in CTF13 mRNA expression... - SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiaeD J Demarini
Laboratory of Genetics, University of Wisconsin, Madison 53706
Mol Cell Biol 12:2154-64. 1992..The mutation that creates the temperature-sensitive sen1-1 allele is located within this 500-amino-acid region, and it causes a substitution for an amino acid that is conserved in all three proteins... - Gene products that promote mRNA turnover in Saccharomyces cerevisiaeP Leeds
Laboratory of Genetics, University of Wisconsin, Madison 53706
Mol Cell Biol 12:2165-77. 1992..On the basis of these results, we suggest that the products of at least two genes, UPF1 and UPF3, are responsible for the accelerated rate of mRNA decay associated with premature translational termination... - The yeast homolog to mouse Tcp-1 affects microtubule-mediated processesD Ursic
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
Mol Cell Biol 11:2629-40. 1991..We also observed that strains carrying tcp1-1 were hypersensitive to antimitotic compounds. Taken together, these observations imply that the TCP1 protein affects microtubule-mediated processes... - Yeast casein kinase I homologues: an essential gene pairL C Robinson
University of Wisconsin, Madison 53706
Proc Natl Acad Sci U S A 89:28-32. 1992..Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues... - SUF12 suppressor protein of yeast. A fusion protein related to the EF-1 family of elongation factorsP G Wilson
Laboratory of Genetics, University of Wisconsin, Madison 53706
J Mol Biol 199:559-73. 1988..We propose a model of suf12-mediated frameshift and nonsense suppression that is based on a primary defect in the normal process of codon recognition... - Navigating without a road mapMichael R Culbertson
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 177:1-7. 2007 - COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiaeD S Conklin
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
Mol Cell Biol 12:3678-88. 1992..The increased tolerance of strains containing multiple copies of the COT1 gene is probably due to increased compartmentalization or sequestration of the ion within mitochondria... - Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiaeJonathan S Finkel
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 184:107-18. 2010..The results suggest that the Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression... - Nuclear import of Upf3p is mediated by importin-alpha/-beta and export to the cytoplasm is required for a functional nonsense-mediated mRNA decay pathway in yeastRenee L Shirley
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 161:1465-82. 2002..To explain these results, we suggest that the mutations in NES-A that impair nuclear export cause additional defects in the function of Upf3p that are not rectified by restoration of export alone... - Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processingDoris Ursic
Laboratories of Molecular Biology and Genetics, R M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
Nucleic Acids Res 32:2441-52. 2004..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways... - Ebs1p, a negative regulator of gene expression controlled by the Upf proteins in the yeast Saccharomyces cerevisiaeAmanda S Ford
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
Eukaryot Cell 5:301-12. 2006..Finally, EBS1 transcript levels are under the control of nonsense-mediated mRNA decay (NMD), providing the first example of an NMD-sensitive transcript whose protein product influences a step in gene expression required for NMD... - Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiaeWei Zheng
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Genetics 180:1391-405. 2008..Overall, our results suggest a model in which ash1 mRNAs are insensitive to NMD while translation is repressed during transport but become sensitive once repression is relieved... - Detecting phosphorylation-dependent interactions with the C-terminal domain of RNA polymerase II subunit Rpb1p using a yeast two-hybrid assayDoris Ursic
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
RNA Biol 5:1-4. 2008..Interactions between the CTD and three known CTD-binding proteins were analyzed. The results suggest that the modified two-hybrid system accurately assays CTD-binding and provides a new and convenient assay for CTD-binding proteins... - Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeastQiaoning Guan
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin, United States of America
PLoS Genet 2:e203. 2006..Furthermore, the methods for analyzing decay rates on a global scale offer a blueprint for new ways to study mRNA decay pathways in any organism where cultured cell lines are available... - Transcript selection and the recruitment of mRNA decay factors for NMD in Saccharomyces cerevisiaeMichael R Culbertson
RNA 11:1333-9. 2005..of NMD to simultaneously reduce or eliminate the synthesis of truncated proteins produced by nonsense transcripts while down-regulating but not completely eliminating functional proteins produced from error-free NMD-sensitive transcripts..